Shawichi Iwamuro

Summary

Affiliation: Toho University
Country: Japan

Publications

  1. ncbi request reprint Teratogenic and anti-metamorphic effects of bisphenol A on embryonic and larval Xenopus laevis
    Shawichi Iwamuro
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Gen Comp Endocrinol 133:189-98. 2003
  2. ncbi request reprint Effects of bisphenol A on thyroid hormone-dependent up-regulation of thyroid hormone receptor alpha and beta and down-regulation of retinoid X receptor gamma in Xenopus tail culture
    Shawichi Iwamuro
    Department of Biology, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Life Sci 79:2165-71. 2006
  3. ncbi request reprint Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii
    Hiroe Suzuki
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama Funabashi, Chiba 274 8510, Japan
    Peptides 28:505-14. 2007
  4. doi request reprint Differential expression of genes encoding preprobrevinin-2, prepropalustrin-2, and preproranatuerin-2 in developing larvae and adult tissues of the mountain brown frog Rana ornativentris
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba, Japan
    Comp Biochem Physiol C Toxicol Pharmacol 151:122-30. 2010
  5. doi request reprint Cloning and expression of genes enocoding antimicrobial peptides and bradykinin from the skin and brain of Oki Tago's brown frog, Rana tagoi okiensis
    Shoro Tazato
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 31:1480-7. 2010
  6. ncbi request reprint Evidence that the genes encoding the melittin-related peptides in the skins of the Japanese frogs Rana sakuraii and Rana tagoi are not orthologous to bee venom melittin genes: developmental- and tissue-dependent gene expression
    Hiroe Suzuki
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 28:2061-8. 2007
  7. ncbi request reprint Antimicrobial peptides from the skin of the Japanese mountain brown frog Rana ornativentris: evidence for polymorphism among preprotemporin mRNAs
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 28:524-32. 2007
  8. ncbi request reprint Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus
    Shuu Morita
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 48:75-82. 2013
  9. ncbi request reprint Molecular cloning and sequence analyses of preprotemporin mRNAs containing premature stop codons from extradermal tissues of Rana tagoi
    Shawichi Iwamuro
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 27:2124-8. 2006
  10. doi request reprint Developmental and thyroid hormone-induced expression of preprotemporin genes in the skin of Japanese mountain brown frog Rana ornativentris
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan
    Ann N Y Acad Sci 1163:494-6. 2009

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Teratogenic and anti-metamorphic effects of bisphenol A on embryonic and larval Xenopus laevis
    Shawichi Iwamuro
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Gen Comp Endocrinol 133:189-98. 2003
    ..Furthermore, BPA suppressed thyroid hormone receptor (TR) beta gene expression both in vivo and in vitro. Thus, we concluded that BPA at the concentrations examined affects both embryonic development and larval metamorphosis...
  2. ncbi request reprint Effects of bisphenol A on thyroid hormone-dependent up-regulation of thyroid hormone receptor alpha and beta and down-regulation of retinoid X receptor gamma in Xenopus tail culture
    Shawichi Iwamuro
    Department of Biology, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Life Sci 79:2165-71. 2006
    ....
  3. ncbi request reprint Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii
    Hiroe Suzuki
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama Funabashi, Chiba 274 8510, Japan
    Peptides 28:505-14. 2007
    ..sakuraii dermal peptides with those of Tago's brown frog Rana tagoi provides evidence for a close phylogenetic relationship between these species...
  4. doi request reprint Differential expression of genes encoding preprobrevinin-2, prepropalustrin-2, and preproranatuerin-2 in developing larvae and adult tissues of the mountain brown frog Rana ornativentris
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba, Japan
    Comp Biochem Physiol C Toxicol Pharmacol 151:122-30. 2010
    ..These results indicate that the expression of amphibian AMP genes is correlated with metamorphosis but is subjected to differential regulation...
  5. doi request reprint Cloning and expression of genes enocoding antimicrobial peptides and bradykinin from the skin and brain of Oki Tago's brown frog, Rana tagoi okiensis
    Shoro Tazato
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 31:1480-7. 2010
    ..NH(2)) displayed antimicrobial or hemolytic activities...
  6. ncbi request reprint Evidence that the genes encoding the melittin-related peptides in the skins of the Japanese frogs Rana sakuraii and Rana tagoi are not orthologous to bee venom melittin genes: developmental- and tissue-dependent gene expression
    Hiroe Suzuki
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 28:2061-8. 2007
    ..In R. tagoi, preproMRP mRNA was not detected in skin prior to the onset of metamorphosis, but its level increased markedly during metamorphosis reaching a maximum at the stages of metamorphic climax...
  7. ncbi request reprint Antimicrobial peptides from the skin of the Japanese mountain brown frog Rana ornativentris: evidence for polymorphism among preprotemporin mRNAs
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 28:524-32. 2007
    ....
  8. ncbi request reprint Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus
    Shuu Morita
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 48:75-82. 2013
    ....
  9. ncbi request reprint Molecular cloning and sequence analyses of preprotemporin mRNAs containing premature stop codons from extradermal tissues of Rana tagoi
    Shawichi Iwamuro
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 27:2124-8. 2006
    ..5 microM) and producing hemolysis of human erythrocytes (LD50 = 50 microM)...
  10. doi request reprint Developmental and thyroid hormone-induced expression of preprotemporin genes in the skin of Japanese mountain brown frog Rana ornativentris
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan
    Ann N Y Acad Sci 1163:494-6. 2009
    ..Treatment of adult animals with 2 x 10(-9) mol/L T(3) for 48 h raised the preprotemporin mRNA levels in skin by 1.5-fold compared with untreated controls...
  11. ncbi request reprint Expression of genes encoding antimicrobial peptides in the Harderian gland of the bullfrog Lithobates catesbeianus
    Itaru Hasunuma
    Department of Biology, Faculty of Education and Integrated Arts and Sciences, Center for Advanced Biomedical Sciences, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Comp Biochem Physiol C Toxicol Pharmacol 152:301-5. 2010
    ....
  12. doi request reprint Antimicrobial action of histone H2B in Escherichia coli: evidence for membrane translocation and DNA-binding of a histone H2B fragment after proteolytic cleavage by outer membrane proteinase T
    Hiroaki Kawasaki
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan
    Biochimie 90:1693-702. 2008
    ....
  13. doi request reprint Molecular cloning and multifunctional characterization of host defense peptides from the bullfrog Harderian gland with special reference to catesbeianalectin
    Yuki Konishi
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Zoolog Sci 30:185-91. 2013
    ..Our findings strongly suggest that these multifunctional host defense peptides in the amphibian HG are involved in innate immunodefense of the eye of the host against pathogenic environmental microorganisms...
  14. ncbi request reprint Molecular cloning of a cDNA encoding atypical antimicrobial and cytotoxic Brevinin-1Ja from the skin of the Japanese brown frog, Rana japonica
    Takumi Koyama
    Department of Biology, Faculty of Science, Toho University, Chiba, Japan
    Zoolog Sci 25:487-91. 2008
    ....
  15. ncbi request reprint Characterization of a hemolytic protein, identified as histone H4, from the skin of the Japanese tree frog Hyla japonica (Hylidae)
    Hiroaki Kawasaki
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274 8510, Japan
    Comp Biochem Physiol B Biochem Mol Biol 149:120-5. 2008
    ..japonica histone H4 gene, which acts as a transcription termination signal, contained a nucleotide sequence (5'-GGCTCTCCTCAGAGCC-3') with unusual structural features not seen in other histone genes...
  16. doi request reprint An efficient protocol for DNA amplification of multiple amphibian skin antimicrobial peptide cDNAs
    Shawichi Iwamuro
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan
    Methods Mol Biol 615:159-76. 2010
    ..With this strategy in mind we have established an efficient protocol suitable for amplification of multiple cDNAs encoding amphibian AMP precursor proteins...
  17. doi request reprint Molecular cloning and characterization of cDNAs encoding biosynthetic precursors for the antimicrobial peptides japonicin-1Ja, japonicin-2Ja, and temporin-1Ja in the Japanese brown frog, Rana japonica
    Takumi Koyama
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Zoolog Sci 28:339-47. 2011
    ..0, respectively. The presence of preprojaponicin-2Ja mRNAs in the cytoplasm of glandular cells in R. japonica dorsal skin glands was demonstrated by means of in situ hybridization using digoxigenin-labeled cRNA probes for the precursor...
  18. ncbi request reprint Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales
    Kyoichi Azuma
    Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, Tokyo 169 8050, Japan
    Biochem Biophys Res Commun 362:594-600. 2007
    ..Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale...
  19. ncbi request reprint Developmental and triiodothyronine-induced expression of genes encoding preprotemporins in the skin of Tago's brown frog Rana tagoi
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Gen Comp Endocrinol 146:242-50. 2006
    ....
  20. doi request reprint Antimicrobial properties of arginine- and lysine-rich histones and involvement of bacterial outer membrane protease T in their differential mode of actions
    Chihiro Tagai
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba, Japan
    Peptides 32:2003-9. 2011
    ..Consequently, it is suggested that OmpT is involved in the antimicrobial properties of Arg- and Lys-rich histones and that the modes of antimicrobial action of these histones are different...
  21. doi request reprint Demonstration of the proopiomelanocortin signaling system in the primary immune organ of the quail
    Tetsuya Kobayashi
    Department of Regulation Biology, Faculty of Science, Saitama University, Saitama, Japan
    Ann N Y Acad Sci 1163:441-3. 2009
    ..The observation of MCR expression within the BF suggests that the alpha-MSH substances may exert paracrine actions within the BF...
  22. ncbi request reprint A protein with antimicrobial activity in the skin of Schlegel's green tree frog Rhacophorus schlegelii (Rhacophoridae) identified as histone H2B
    Hiroaki Kawasaki
    Department of Biology, Faculty of Science, Toho University, Funabashi, 274 8510, Chiba, Japan
    Biochem Biophys Res Commun 312:1082-6. 2003
    ..The data provide further evidence that histones play a role in the defense against microorganisms...
  23. ncbi request reprint Antimicrobial peptides from the skin of the Tsushima brown frog Rana tsushimensis
    J Michael Conlon
    Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates
    Comp Biochem Physiol C Toxicol Pharmacol 143:42-9. 2006
    ..The peptide therefore represents a candidate for drug development...
  24. ncbi request reprint A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties
    J Michael Conlon
    Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain 17666, United Arab Emirates
    Biochem Biophys Res Commun 306:496-500. 2003
    ..However, at non-cytotoxic concentrations (<or=8 microM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection...
  25. ncbi request reprint Expression of the estrogen receptor alpha gene in the anal fin of Japanese medaka, Oryzias latipes, by environmental concentrations of bisphenol A
    Hitomi Hayashi
    J Toxicol Sci 32:91-6. 2007
    ..3- and 3.3-fold with 1 day of exposure, respectively. The present results showed that medaka anal fins may be a sensitive bio-indicator for screening of environmental estrogenic chemicals...
  26. ncbi request reprint A family of acyclic brevinin-1 peptides from the skin of the Ryukyu brown frog Rana okinavana
    J Michael Conlon
    Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates
    Peptides 26:185-90. 2005
    ....
  27. ncbi request reprint A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica
    J Michael Conlon
    Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates
    Regul Pept 118:135-41. 2004
    ..NH(2)) and temporin-1PRb (ILPILGNLLNSLL.NH(2)) that atypically lacked basic amino acid residues and showed only very weak antimicrobial and hemolytic activity...
  28. ncbi request reprint Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii
    J Michael Conlon
    Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates
    Toxicon 50:746-56. 2007
    ..okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes...
  29. ncbi request reprint Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica
    Todd Isaacson
    Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178, USA
    Peptides 23:419-25. 2002
    ..coli were japonicin-1, 30 microM; japonicin-2, 12 microM; and temporin-1Ja > 100 microM. The MICs against S. aureus were japonicin-1, > 100 microM; japonicin-2, 20 microM; and temporin-1Ja, > 100 microM...
  30. doi request reprint A protocol for immunoaffinity separation of the accumulated ubiquitin-protein conjugates solubilized with sodium dodecyl sulfate
    Yohta Shimada
    Department of Biochemistry, Jikei University School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
    Anal Biochem 377:77-82. 2008
    ..These results indicate that this simple protocol has potential applications for excellent immunoaffinity separation of the less-soluble proteins in diverse cells and tissues...