Keishin Sugawara

Summary

Affiliation: The Chemo-Sero-Therapeutic Research Institute
Country: Japan

Publications

  1. ncbi request reprint Development of Vero cell-derived inactivated Japanese encephalitis vaccine
    Keishin Sugawara
    Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kumamoto 869 1298, Japan
    Biologicals 30:303-14. 2002
  2. doi request reprint Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S
    Satoshi Koyanagi
    Biologics Development and Production Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
    J Biosci Bioeng 110:597-601. 2010
  3. ncbi request reprint Non-clinical and phase I clinical trials of a Vero cell-derived inactivated Japanese encephalitis vaccine
    Syoji Kuzuhara
    The Chemo Sero Therapeutic Research Institute, Kikuchi Research Center, Kawabe Kyokushi, Kikuchi, Kumamoto 869 1298, Japan
    Vaccine 21:4519-26. 2003
  4. ncbi request reprint Purification and cDNA cloning of a novel protease inhibitor secreted into culture supernatant by MDCK cells
    Kiyoto Nishiyama
    Applied Research Department, Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kyokushi Kawabe 1314 1, Kikuchi, Kumamoto 869 1298, Japan
    Biologicals 36:122-33. 2008
  5. doi request reprint Large-scale production of major house dust mite allergen der f 2 mutant (C8/119S) in Escherichia coli
    Satoshi Koyanagi
    Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
    J Biosci Bioeng 106:387-92. 2008
  6. ncbi request reprint Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin
    Hiroshi Yonemura
    Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, Kawabe, Kyokushi, Kikuchi, Kumamoto 869 1298
    J Biochem 135:577-82. 2004
  7. doi request reprint Zernike phase contrast electron microscopy of ice-embedded influenza A virus
    Masashi Yamaguchi
    Medical Mycology Research Center, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
    J Struct Biol 162:271-6. 2008

Collaborators

  • Kiyoto Nishiyama
  • Motoharu Abe
  • Satoshi Koyanagi
  • K Soejima
  • Masashi Yamaguchi
  • Hiroshi Yonemura
  • Syoji Kuzuhara
  • Hiroshi Nakatake
  • Yo Nakahara
  • Wataru Morikawa
  • Tomohiro Nakagaki
  • Chikateru Nozaki
  • Yasuharu Kamachi
  • Yoshitaka Ushio
  • Takayuki Imamura
  • Yoichiro Kino
  • Kengo Sonoda
  • Hiroshi Mizokami
  • Kengo Takeda
  • Junko Obata
  • Tomitaka Honda
  • Kohji Ueda
  • Takamichi Shigaki
  • Kyosuke Mizuno
  • Hajime Matsui
  • Hideki Nakamura
  • Masahiko Tanaka
  • Kenshi Hayashida

Detail Information

Publications7

  1. ncbi request reprint Development of Vero cell-derived inactivated Japanese encephalitis vaccine
    Keishin Sugawara
    Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kumamoto 869 1298, Japan
    Biologicals 30:303-14. 2002
    ..Our system is very simple and could be easily scaled-up to allow vaccine production at a several thousand litre scale...
  2. doi request reprint Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S
    Satoshi Koyanagi
    Biologics Development and Production Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
    J Biosci Bioeng 110:597-601. 2010
    ..In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity...
  3. ncbi request reprint Non-clinical and phase I clinical trials of a Vero cell-derived inactivated Japanese encephalitis vaccine
    Syoji Kuzuhara
    The Chemo Sero Therapeutic Research Institute, Kikuchi Research Center, Kawabe Kyokushi, Kikuchi, Kumamoto 869 1298, Japan
    Vaccine 21:4519-26. 2003
    ....
  4. ncbi request reprint Purification and cDNA cloning of a novel protease inhibitor secreted into culture supernatant by MDCK cells
    Kiyoto Nishiyama
    Applied Research Department, Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kyokushi Kawabe 1314 1, Kikuchi, Kumamoto 869 1298, Japan
    Biologicals 36:122-33. 2008
    ..These results suggest that the inhibitory factors are secreted from MDCK cells, which are involved in prevention of virus replication, and applicable to the protection of host cells from virus infection...
  5. doi request reprint Large-scale production of major house dust mite allergen der f 2 mutant (C8/119S) in Escherichia coli
    Satoshi Koyanagi
    Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
    J Biosci Bioeng 106:387-92. 2008
    ..pWU11-C8/119S/HB101 can be used as an easy, low cost expression system on a large scale. It is also advantageous for industrial production in that the addition of an inducer is not required to achieve expression of the mite allergen...
  6. ncbi request reprint Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin
    Hiroshi Yonemura
    Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, Kawabe, Kyokushi, Kikuchi, Kumamoto 869 1298
    J Biochem 135:577-82. 2004
    ..Our system is suitable for the large-scale production of recombinant alpha-thrombin, which can be used in place of clinically available alpha-thrombin derived from human or bovine plasma...
  7. doi request reprint Zernike phase contrast electron microscopy of ice-embedded influenza A virus
    Masashi Yamaguchi
    Medical Mycology Research Center, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
    J Struct Biol 162:271-6. 2008
    ....