Affiliation: The Chemo-Sero-Therapeutic Research Institute
- Development of Vero cell-derived inactivated Japanese encephalitis vaccineKeishin Sugawara
Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kumamoto 869 1298, Japan
Biologicals 30:303-14. 2002..Our system is very simple and could be easily scaled-up to allow vaccine production at a several thousand litre scale...
- Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119SSatoshi Koyanagi
Biologics Development and Production Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
J Biosci Bioeng 110:597-601. 2010..In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity...
- Non-clinical and phase I clinical trials of a Vero cell-derived inactivated Japanese encephalitis vaccineSyoji Kuzuhara
The Chemo Sero Therapeutic Research Institute, Kikuchi Research Center, Kawabe Kyokushi, Kikuchi, Kumamoto 869 1298, Japan
Vaccine 21:4519-26. 2003....
- Purification and cDNA cloning of a novel protease inhibitor secreted into culture supernatant by MDCK cellsKiyoto Nishiyama
Applied Research Department, Kikuchi Research Center, The Chemo Sero Therapeutic Research Institute, Kyokushi Kawabe 1314 1, Kikuchi, Kumamoto 869 1298, Japan
Biologicals 36:122-33. 2008..These results suggest that the inhibitory factors are secreted from MDCK cells, which are involved in prevention of virus replication, and applicable to the protection of host cells from virus infection...
- Large-scale production of major house dust mite allergen der f 2 mutant (C8/119S) in Escherichia coliSatoshi Koyanagi
Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, 1314 1 Kyokushikawabe, Kikuchi, Kumamoto 869 1298, Japan
J Biosci Bioeng 106:387-92. 2008..pWU11-C8/119S/HB101 can be used as an easy, low cost expression system on a large scale. It is also advantageous for industrial production in that the addition of an inducer is not required to achieve expression of the mite allergen...
- Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarinHiroshi Yonemura
Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, Kawabe, Kyokushi, Kikuchi, Kumamoto 869 1298
J Biochem 135:577-82. 2004..Our system is suitable for the large-scale production of recombinant alpha-thrombin, which can be used in place of clinically available alpha-thrombin derived from human or bovine plasma...
- Zernike phase contrast electron microscopy of ice-embedded influenza A virusMasashi Yamaguchi
Medical Mycology Research Center, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
J Struct Biol 162:271-6. 2008....