Haruhiko Siomi

Summary

Affiliation: The University of Tokushima
Country: Japan

Publications

  1. ncbi Interactions between transposable elements and Argonautes have (probably) been shaping the Drosophila genome throughout evolution
    Haruhiko Siomi
    Institute for Genome Research, The University of Tokushima, 3 18 15 Kuramoto, Tokushima 770 8503, Japan
    Curr Opin Genet Dev 18:181-7. 2008
  2. ncbi [RNAi, Fire (see text) Mello]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 51:2352-5. 2006
  3. ncbi Expanding RNA physiology: microRNAs in a unicellular organism
    Haruhiko Siomi
    Institute for Genome Research, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8503 Japan
    Genes Dev 21:1153-6. 2007
  4. ncbi [Role of non-coding RNAs in genome network]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 49:2970-3. 2004
  5. ncbi RNA interference: a new mechanism by which FMRP acts in the normal brain? What can Drosophila teach us?
    Haruhiko Siomi
    Institute for Genome Research, University of Tokushima, Kuramoto, Tokushima, Japan
    Ment Retard Dev Disabil Res Rev 10:68-74. 2004
  6. ncbi [Non-coding RNAs as spatiotemporal regulators of genome network]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 49:2503-9. 2004
  7. ncbi Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells
    Yoshinori Kawamura
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Nature 453:793-7. 2008
  8. ncbi A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila
    Lalith S Gunawardane
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Science 315:1587-90. 2007
  9. ncbi Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad
    Kazumichi M Nishida
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    RNA 13:1911-22. 2007
  10. ncbi A potential link between transgene silencing and poly(A) tails
    Mikiko C Siomi
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    RNA 11:1004-11. 2005

Collaborators

Detail Information

Publications27

  1. ncbi Interactions between transposable elements and Argonautes have (probably) been shaping the Drosophila genome throughout evolution
    Haruhiko Siomi
    Institute for Genome Research, The University of Tokushima, 3 18 15 Kuramoto, Tokushima 770 8503, Japan
    Curr Opin Genet Dev 18:181-7. 2008
    ..Complex interactions between TEs and RNA silencing machineries have been co-opted to regulate cellular genes...
  2. ncbi [RNAi, Fire (see text) Mello]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 51:2352-5. 2006
  3. ncbi Expanding RNA physiology: microRNAs in a unicellular organism
    Haruhiko Siomi
    Institute for Genome Research, University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8503 Japan
    Genes Dev 21:1153-6. 2007
  4. ncbi [Role of non-coding RNAs in genome network]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 49:2970-3. 2004
  5. ncbi RNA interference: a new mechanism by which FMRP acts in the normal brain? What can Drosophila teach us?
    Haruhiko Siomi
    Institute for Genome Research, University of Tokushima, Kuramoto, Tokushima, Japan
    Ment Retard Dev Disabil Res Rev 10:68-74. 2004
    ....
  6. ncbi [Non-coding RNAs as spatiotemporal regulators of genome network]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 49:2503-9. 2004
  7. ncbi Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells
    Yoshinori Kawamura
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Nature 453:793-7. 2008
    ..Mutations in Dicer-2 caused an increase in retrotransposon transcripts. Together, our findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila...
  8. ncbi A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila
    Lalith S Gunawardane
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Science 315:1587-90. 2007
    ..These data support a model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI...
  9. ncbi Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad
    Kazumichi M Nishida
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    RNA 13:1911-22. 2007
    ..These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes...
  10. ncbi A potential link between transgene silencing and poly(A) tails
    Mikiko C Siomi
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    RNA 11:1004-11. 2005
    ..The possibility that AGO2 and Dicer2 involve, at least in part, poly(A) length maintenance of transgene mRNA suggests a potentially important link between transgene silencing and poly(A) tails...
  11. ncbi Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends
    Kuniaki Saito
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    Genes Dev 21:1603-8. 2007
    ..Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2'-O-methylation in Drosophila...
  12. ncbi Slicer function of Drosophila Argonautes and its involvement in RISC formation
    Keita Miyoshi
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Genes Dev 19:2837-48. 2005
    ..These findings give rise to a new model in which AGO2 is directly involved in RISC formation as "Slicer" of the passenger strand of the siRNA duplex...
  13. ncbi In vitro RNA cleavage assay for Argonaute-family proteins
    Keita Miyoshi
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    Methods Mol Biol 442:29-43. 2008
    ..We describe (in vitro) Slicer assays using endogenous Argonaute protein immunopurified from animal cells and recombinant Argonaute protein produced in and purified from Escherichia coli...
  14. ncbi Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome
    Kuniaki Saito
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Genes Dev 20:2214-22. 2006
    ..In vitro, recombinant Piwi exhibits target RNA cleavage activity. These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets...
  15. ncbi Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells
    Kuniaki Saito
    Institute for Genome Research, University of Tokushima, Kuramoto, Tokushima, Japan
    PLoS Biol 3:e235. 2005
    ..These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs...
  16. ncbi Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways
    Katsutomo Okamura
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Genes Dev 18:1655-66. 2004
    ..Our findings show that distinct Argonaute proteins act at different steps of the small RNA silencing mechanism and suggest that there are inherent differences between siRNA-initiated RISCs and miRNA-initiated RISCs in Drosophila...
  17. ncbi Casein kinase II phosphorylates the fragile X mental retardation protein and modulates its biological properties
    Mikiko C Siomi
    Institute for Genome Research, University of Tokushima, Kuramoto, Tokushima 770 8503, Japan
    Mol Cell Biol 22:8438-47. 2002
    ..These studies support the model that the biological functions of FMR1, such as regulation of gene expression, are likely regulated by its phosphorylation...
  18. ncbi A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins
    Akira Ishizuka
    Institute for Genome Research, Graduate School of Nutrition, University of Tokushima, Tokushima 770 8503, Japan
    Genes Dev 16:2497-508. 2002
    ..Our findings suggest a model in which the RNAi and dFMR1-mediated translational control pathways intersect in Drosophila. Our findings also raise the possibility that defects in an RNAi-related machinery may cause human disease...
  19. ncbi In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates
    Akira Ishizuka
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    Methods Mol Biol 342:277-86. 2006
    ..In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials...
  20. ncbi Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing
    Asuka Azuma-Mukai
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Proc Natl Acad Sci U S A 105:7964-9. 2008
    ..The hAgo antibodies we raised are valuable tools for ascertaining the functional behavior of endogenous Argonautes and miRNAs in RNA silencing...
  21. ncbi Identification of components of RNAi pathways using the tandem affinity purification method
    Mikiko C Siomi
    Institute for Genome Research, University of Tokushima, Japan
    Methods Mol Biol 309:1-9. 2005
  22. ncbi [Fragile X syndrome, a disease caused by defects in an RNA-binding protein]
    Mikiko C Siomi
    Tanpakushitsu Kakusan Koso 48:480-6. 2003
  23. ncbi Overexpression of HMGA2 relates to reduction of the let-7 and its relationship to clinicopathological features in pituitary adenomas
    Zhi Rong Qian
    Department of Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School of Medicine, Tokushima, Japan
    Mod Pathol 22:431-41. 2009
    ..These findings support a causal link between let-7 and high-mobility group A2 whereby loss of let-7 expression induces high-mobility group A2 upregulation that represents an important mechanism in pituitary tumorigenesis and progression...
  24. ncbi A role for the Drosophila fragile X-related gene in circadian output
    Shunsuke Inoue
    Institute for Genome Research, University of Tokushima, Kuramoto 3 18 15, 770 8503, Tokushima, Japan
    Curr Biol 12:1331-5. 2002
    ..In addition, the clock-controlled eclosion rhythm is normal in DFMR1-deficient flies. These results suggest that DFMR1 plays a critical role in the circadian output pathway regulating locomotor activity in Drosophila...
  25. ncbi [Functions and dynamics of nuclear non-coding RNAs]
    Kuniaki Saito
    Tanpakushitsu Kakusan Koso 51:2220-5. 2006
  26. ncbi How selfish retrotransposons are silenced in Drosophila germline and somatic cells
    Mikiko C Siomi
    Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    FEBS Lett 582:2473-8. 2008
    ..In this review, we address RNA silencing mechanisms that silence retrotransposons, a subset of TEs, and discuss how germline and somatic cells are equipped with different retrotransposon silencing mechanisms...
  27. ncbi The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans
    Hiroaki Tabara
    Program in Molecular Medicine, University of Massachusetts Meidcal School, Worcester, MA 1605, USA
    Cell 109:861-71. 2002
    ..Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing...