S Omura

Summary

Affiliation: The Kitasato Institute
Country: Japan

Publications

  1. ncbi EM703 improves bleomycin-induced pulmonary fibrosis in mice by the inhibition of TGF-beta signaling in lung fibroblasts
    Ying Ji Li
    Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan
    Respir Res 7:16. 2006
  2. ncbi An anthelmintic compound, nafuredin, shows selective inhibition of complex I in helminth mitochondria
    S Omura
    Research Center for Biological Function, The Kitasato Institute, Shirokane, Minato ku, Tokyo 108 8642, Japan
    Proc Natl Acad Sci U S A 98:60-2. 2001
  3. ncbi Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites
    S Omura
    The Kitasato Institute for Life Sciences, Kitasato University, Tokyo 108 8642, Japan
    Proc Natl Acad Sci U S A 98:12215-20. 2001
  4. ncbi The life and times of ivermectin - a success story
    Satoshi Omura
    Kitasato Institute, 5 9 1 Shirokane, Minato ku, Tokyo 108 8642, Japan
    Nat Rev Microbiol 2:984-9. 2004
  5. ncbi Ivermectin: 25 years and still going strong
    S Omura
    The Kitasato Institute, Tokyo, Japan
    Int J Antimicrob Agents 31:91-8. 2008
  6. ncbi Nafuredin, a novel inhibitor of NADH-fumarate reductase, produced by Aspergillus niger FT-0554
    H Ui
    Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 54:234-8. 2001
  7. ncbi Total synthesis of nafuredin, a selective NADH-fumarate reductase inhibitor
    D Takano
    School of Pharmaceutical Science, Kitasato University, Kitasato Institute for Life Sciences, Kitasato University, and CREST, The Japan Science and Technology Corporation (JST, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Org Lett 3:2289-91. 2001
  8. ncbi Cloning of the gene encoding avermectin B 5-O-methyltransferase in avermectin-producing Streptomyces avermitilis
    H Ikeda
    School of Pharmaceutical Sciences, Kitasato University, Minato ku, Tokyo 108, Japan
    Gene 206:175-80. 1998
  9. ncbi Deacetylravidomycin M, a new inhibitor of IL-4 signal transduction, produced by Streptomyces sp. WK-6326. II. Structure elucidation
    M Arai
    The Kitasato Institute, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 54:562-6. 2001
  10. ncbi Organization of biosynthetic gene cluster for avermectin in Streptomyces avermitilis: analysis of enzymatic domains in four polyketide synthases
    H Ikeda
    School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    J Ind Microbiol Biotechnol 27:170-6. 2001

Collaborators

Detail Information

Publications58

  1. ncbi EM703 improves bleomycin-induced pulmonary fibrosis in mice by the inhibition of TGF-beta signaling in lung fibroblasts
    Ying Ji Li
    Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan
    Respir Res 7:16. 2006
    ..We investigated the anti-inflammatory and antifibrotic effects of EM703 in an experimental model of bleomycin-induced lung injury and subsequent fibrosis in mice...
  2. ncbi An anthelmintic compound, nafuredin, shows selective inhibition of complex I in helminth mitochondria
    S Omura
    Research Center for Biological Function, The Kitasato Institute, Shirokane, Minato ku, Tokyo 108 8642, Japan
    Proc Natl Acad Sci U S A 98:60-2. 2001
    ..Thus, our study indicates that mitochondrial complex I is a promising target for chemotherapy, and nafuredin is a potential lead compound as an anthelmintic isolated from microorganisms...
  3. ncbi Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites
    S Omura
    The Kitasato Institute for Life Sciences, Kitasato University, Tokyo 108 8642, Japan
    Proc Natl Acad Sci U S A 98:12215-20. 2001
    ..These secondary metabolite clusters are widely located in the genome but half of them are near both ends of the genome. The total length of these clusters occupies about 6.4% of the genome...
  4. ncbi The life and times of ivermectin - a success story
    Satoshi Omura
    Kitasato Institute, 5 9 1 Shirokane, Minato ku, Tokyo 108 8642, Japan
    Nat Rev Microbiol 2:984-9. 2004
    ..The drug is now being used, free of charge, in two global disease-elimination programmes that are benefiting millions of the world's poorest people...
  5. ncbi Ivermectin: 25 years and still going strong
    S Omura
    The Kitasato Institute, Tokyo, Japan
    Int J Antimicrob Agents 31:91-8. 2008
    ....
  6. ncbi Nafuredin, a novel inhibitor of NADH-fumarate reductase, produced by Aspergillus niger FT-0554
    H Ui
    Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 54:234-8. 2001
    ..It was obtained from culture broth of Aspergillus niger FT-0554 isolated from a marine sponge. The structure was elucidated as an epoxy-delta-lactone with an attached methylated olefinic side chain on the basis of spectral analysis...
  7. ncbi Total synthesis of nafuredin, a selective NADH-fumarate reductase inhibitor
    D Takano
    School of Pharmaceutical Science, Kitasato University, Kitasato Institute for Life Sciences, Kitasato University, and CREST, The Japan Science and Technology Corporation (JST, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Org Lett 3:2289-91. 2001
    ..The C1-C8 and C9-C18 segments were derived efficiently from D-glucose and (S)-(-)-2-methyl-1-butanol, respectively, coupled by stereoselective Julia olefination, and converted to nafuredin...
  8. ncbi Cloning of the gene encoding avermectin B 5-O-methyltransferase in avermectin-producing Streptomyces avermitilis
    H Ikeda
    School of Pharmaceutical Sciences, Kitasato University, Minato ku, Tokyo 108, Japan
    Gene 206:175-80. 1998
    ..Sequence analysis of the aveD region of the mutant strain revealed that a point mutation is within ORF, being Thr23-->Ile substitution. This mutation causes the inactivation of O-methyltransferase activity of AveD...
  9. ncbi Deacetylravidomycin M, a new inhibitor of IL-4 signal transduction, produced by Streptomyces sp. WK-6326. II. Structure elucidation
    M Arai
    The Kitasato Institute, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 54:562-6. 2001
    ....
  10. ncbi Organization of biosynthetic gene cluster for avermectin in Streptomyces avermitilis: analysis of enzymatic domains in four polyketide synthases
    H Ikeda
    School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    J Ind Microbiol Biotechnol 27:170-6. 2001
    ..AVES1 and 2 contain two and four modules, respectively, whereas AVES 3 and AVES 4 each contains three modules. The 12 modules correspond to the 12 cycles required for synthesis of the avermectin aglycon...
  11. ncbi Cladospolide D, a new 12-membered macrolide antibiotic produced by Cladosporium sp. FT-0012
    H Zhang
    The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 54:635-41. 2001
    ..The structure of cladospolide D was deduced to be (E)-2-dodecen-5-hydroxy-11-olide-4-one. Cladospolide D showed antifungal activity against Pyricularia oryzae and Mucor racemosus...
  12. ncbi Deacetylravidomycin M, a new inhibitor of IL-4 signal transduction, produced by Streptomyces sp. WK-6326. I. Taxonomy, fermentation, isolation and biological activities
    M Arai
    The Kitasato Institute, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 54:554-61. 2001
    ..Deacetylravidomycin M inhibited IL-4-induced CD23 expression in U937 cells without any cytotoxic effect, whereas deacetylravidomycin showed no inhibitory activity...
  13. ncbi A quinol peroxidase inhibitor prevents secretion of a leukotoxin from Aggregatibacter actinomycetemcomitans
    E Takashima
    Department of Microbiology, School of Life Dentistry at Tokyo, The Nippon Dental University, Chiyoda ku, Tokyo, Japan
    J Periodontal Res 45:123-8. 2010
    ..In the present study, we aimed to find a highly potent QPO inhibitor to attenuate the virulence of A. actinomycetemcomitans...
  14. ncbi Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis
    H Ikeda
    School of Pharmaceutical Sciences, Kitasato University, Tokyo 108 8641, Japan
    Proc Natl Acad Sci U S A 96:9509-14. 1999
    ....
  15. ncbi Potent antimalarial activities of polyether antibiotic, X-206
    K Otoguro
    Research Center for Tropical Diseases, Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 54:658-63. 2001
    ..These observations are the first report of antimalarial activity of X-206...
  16. ncbi Structure-specific inhibition of cholesteryl ester transfer protein by azaphilones
    H Tomoda
    Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 52:160-70. 1999
    ..4 and 10.3 microM, respectively). A model of the reaction suggested that sclerotiorin reacts with a primary amine of amino acids such as lysine in the protein to form a covalent bond...
  17. ncbi Description of two novel species of the genus Kitasatospora Omura et al. 1982, Kitasatospora cineracea sp. nov. and Kitasatospora niigatensis sp. nov
    K Tajima
    Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan
    Int J Syst Evol Microbiol 51:1765-71. 2001
    ..nov. and Kitasatospora niigatensis sp. nov., with the type strains K. cineracea SK-3255T (= IFO 16452T = JCM 10915T = NRRL B-23134T) and K. niigatensis SK-3406T (= IFO 16453T = JCM 10916T = NRRL B-24135T)...
  18. ncbi Satoshi Omura: in pursuit of nature's bounty
    Andy Crump
    Research Centre for Tropical Diseases, The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan
    Trends Parasitol 21:126-32. 2005
    ..It has also improved the health of pets and livestock around the globe, and encouraged development of a community-based delivery mechanism that could herald a revolution in public health care in Africa...
  19. ncbi Streptomyces avermectinius sp. nov., an avermectin-producing strain
    Yoko Takahashi
    Kitasato Institute for Life Sciences, Kitasato University, 5-9-1, Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Int J Syst Evol Microbiol 52:2163-8. 2002
    ..Analysis of DNA-DNA hybridization tests distinguished strain MA-4680(T) from these eight Streptomyces species. The type strain is strain MA-4680(T) (= ATCC 31267(T) = NRRL 8165(T))...
  20. ncbi Erythromycin suppresses nuclear factor-kappaB and activator protein-1 activation in human bronchial epithelial cells
    M Desaki
    Department of Respiratory Medicine, University of Tokyo Graduate School of Medicine, Tokyo, Japan
    Biochem Biophys Res Commun 267:124-8. 2000
    ..Our findings support the concept that the recruitment of neutrophils in airway diseases may be regulated by NF-kappaB and AP-1...
  21. ncbi Molecular cloning of actinohivin, a novel anti-HIV protein from an actinomycete, and its expression in Escherichia coli
    J Inokoshi
    School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-8641, Japan
    Biochem Biophys Res Commun 281:1261-5. 2001
    ..The actinohivin gene could be expressed in E. coli using a pET30Xa/LIC expression vector and the purified recombinant actinohivin was found to inhibit syncytium formation to a similar extent as actinohivin from its natural source...
  22. ncbi Chlorogentisylquinone, a new neutral sphingomyelinase inhibitor, produced by a marine fungus
    R Uchida
    Kitasato Institute for Life Sciences, Kitasato University and The Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 54:882-9. 2001
    ..Chlorogentisylquinone inhibited neutral sphingomyelinase activity of rat brain membranes with an IC50 value of 1.2 microM...
  23. ncbi Hygromycin A, an antitreponemal substance. II. Therapeutic effect for swine dysentery
    A Nakagawa
    Kitasato Institute, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 40:1627-35. 1987
    ..T. hyodysenteriae-caused swine dysentery was successfully controlled by feeding hygromycin A at 5 g/ton. Hygromycin A medicated pigs performed as well as or better than carbadox-medicated pigs...
  24. ncbi Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. I: Fermentation, isolation and biological properties
    Ryuji Uchida
    Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 63:151-5. 2010
    ..Nosokomycins inhibited the growth of MRSA with MIC values of 0.125 microg ml(-1) using the liquid microdilution method. Furthermore, MRSA-infected silkworms survived when nosokomycin A or B was injected at a dose of 50 microg per larva...
  25. ncbi Sinefungin VA and dehydrosinefungin V, new antitrypanosomal antibiotics produced by Streptomyces sp. K05-0178
    Megumi Niitsuma
    Research Center for Tropical Diseases, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 63:673-9. 2010
    ..0026 μg ml(-1) in vitro without cytotoxicity against MRC-5 cells. Dehydrosinefungin V showed moderate antitrypanosomal activity (IC(50)=0.15 μg ml(-1))...
  26. ncbi Decursin and decursinol angelate selectively inhibit NADH-fumarate reductase of Ascaris suum
    Kazuro Shiomi
    Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
    Planta Med 73:1478-81. 2007
    ..In contrast, decursinol angelate moderately inhibits bovine heart complexes II and III. Decursinol inhibits A. SUUM NFRD to a similar extent, but its target is complex II. It also inhibits bovine heart complexes II and III...
  27. ncbi Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Streptomyces sp. K04-0144. I. Taxonomy, fermentation, isolation and structural elucidation
    Atsushi Fukumoto
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo, Japan
    J Antibiot (Tokyo) 61:1-6. 2008
    ..The structure of cyslabdan was elucidated by spectroscopic analyses including NMR. The compound has a labdane-type diterpene skeleton connecting with an N-acetylcysteine via thioether linkage...
  28. ncbi Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Streptomyces sp. K04-0144. II. Biological activities
    Atsushi Fukumoto
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo, Japan
    J Antibiot (Tokyo) 61:7-10. 2008
    ..Furthermore, among beta-lactam antibiotics, the activity of carbapenems was most remarkably potentiated by cyslabdan...
  29. ncbi Guadinomines, Type III secretion system inhibitors, produced by Streptomyces sp. K01-0509. I: taxonomy, fermentation, isolation and biological properties
    Masato Iwatsuki
    The Kitasato Institute, Japan
    J Antibiot (Tokyo) 61:222-9. 2008
    ..Guadinomines A and B showed potent inhibition with IC50 values of 0.02 and 0.007 microg/ml, respectively, guadinomine D showed moderate activity (IC50: 8.5 microg/ml), while guadinomines C1 and C2 and guadinomic acid had no activity...
  30. ncbi Anti-atherosclerotic activity of triacsin C, an acyl-CoA synthetase inhibitor
    Daisuke Matsuda
    School of Pharmacy, Kitasato University, Shirokane, Tokyo, Japan
    J Antibiot (Tokyo) 61:318-21. 2008
    ..The results strongly suggested that triacsin C shows anti-atherogenic activity by inhibiting acyl-CoA synthetase activity...
  31. ncbi Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. II: Structure elucidation
    Ryuji Uchida
    Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    J Antibiot (Tokyo) 63:157-63. 2010
    ..All nosokomycins lack the cyclopentenone moiety in the oligosaccharide moiety of moenomycin A...
  32. ncbi Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism
    Mamoru Komatsu
    Laboratory of Microbial Engineering, Kitasato Institute for Life Sciences, Kitasato University, 1 15 1 Kitasato Sagamihara, Kanagawa 228 8555, Japan
    Proc Natl Acad Sci U S A 107:2646-51. 2010
    ..These findings highlight the strength and flexibility of engineered S. avermitilis as a model host for heterologous gene expression, resulting in the production of exogenous natural and unnatural metabolites...
  33. ncbi Phenatic acids A and B, new potentiators of antifungal miconazole activity produced by Streptomyces sp. K03-0132
    Takashi Fukuda
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    J Antibiot (Tokyo) 58:252-9. 2005
    ..These compounds potentiate miconazole activity against Candida albicans. Phenatic acid B also showed moderate antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Bacteroides fragilis and Acholeplasma laidlawii...
  34. ncbi A new antibiotic, antimycin Ag, produced by Streptomyces sp. K01-0031
    Kazuro Shiomi
    School of Pharmaceutical Sciences, Kitasato University, Minato ku, Tokyo 108 8641, Japan
    J Antibiot (Tokyo) 58:74-8. 2005
    ..It showed potent nematocidal and insecticidal activities against Caenorhabditis elegans and Artemia salina, respectively. It inhibited bovine heart NADH oxidase at nanomolar level like other known antimycins...
  35. ncbi A gamma-lactone form nafuredin, nafuredin-gamma, also inhibits helminth complex I
    Kazuro Shiomi
    School of Pharmaceutical Sciences, Kitasato University, Minato ku, Tokyo 108 8641, Japan
    J Antibiot (Tokyo) 58:50-5. 2005
    ..Nafuredin-gamma shows similar complex I inhibitory activity as nafuredin, and it also possesses anthelmintic activity in vivo...
  36. ncbi Total synthesis of (-)-borrelidin
    Tohru Nagamitsu
    School of Pharmaceutical Science, Kitasato University, Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Org Lett 6:1865-7. 2004
    ..Et(2)O-mediated chelation-controlled allylation. [reaction: see text]..
  37. ncbi Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis
    Haruo Ikeda
    Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan
    Nat Biotechnol 21:526-31. 2003
    ..In contrast, the terminal regions were not conserved and preferentially contained nonessential genes...
  38. ncbi Guadinomines, Type III secretion system inhibitors, produced by Streptomyces sp. K01-0509. II: physico-chemical properties and structure elucidation
    Masato Iwatsuki
    The Kitasato Institute, Japan
    J Antibiot (Tokyo) 61:230-6. 2008
    ....
  39. ncbi Streptomyces scabrisporus sp. nov
    Xu Ping
    Research Center for Biological Function, The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan
    Int J Syst Evol Microbiol 54:577-81. 2004
    ..On the basis of genomic and physiological properties, the novel species Streptomyces scabrisporus sp. nov. is proposed; the type strain is strain KM-4927(T) (=JCM 11712(T)=NRRL B-24202(T))...
  40. ncbi Synthesis and biological activities of novel 4"-alkylidene avermectin derivatives
    Kenichiro Nagai
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University and The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Bioorg Med Chem Lett 13:3943-6. 2003
    ..Further modifications led to derivatives bearing diverse functional groups. The new avermectin derivatives showed potent growth inhibitory activity against Artemia salina and Caenorhabditis elegans...
  41. ncbi Acylated pregnane glycosides from Caralluma tuberculata and their antiparasitic activity
    Essam Abdel-Sattar
    Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
    Phytochemistry 69:2180-6. 2008
    ..All the isolated compounds were tested for their antimalarial and antitrypanosomal activities as well as their cytotoxicity against human diploid embryonic cell line (MRC5)...
  42. ncbi Lanopylins A1, B1, A2 and B2, novel lanosterol synthase inhibitors from Streptomyces sp. K99-5041
    Yuichi Sakano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    J Antibiot (Tokyo) 56:817-26. 2003
    ..These compounds inhibited recombinant human lanosterol synthase with IC50 values of 15, 18, 33, and 41 microM, respectively...
  43. ncbi Cytochrome p450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2)
    David C Lamb
    Institute of Biological Sciences, University of Wales Aberystwyth, Aberystwyth, UK
    Biochem Biophys Res Commun 307:610-9. 2003
    ..Amongst this range of CYPs are forms associated with avermectin, filipin, geosmin, and pentalenolactone biosynthesis as well as unknown pathways of secondary metabolism...
  44. ncbi Takanawaenes, novel antifungal antibiotics produced by Streptomyces sp. K99-5278. I. Taxonomy, fermentation, isolation and biological properties
    Yong-Pil Kim
    Kitasato Institute for Life Sciences, Kitasato University and The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    J Antibiot (Tokyo) 56:448-53. 2003
    ..K99-5278 by solvent extraction, silica-gel column chromatography and HPLC. Takanawaenes showed antifungal activity against Aspergillus niger, Mucor racemosus, Candida albicans and Saccharomyces cerevisiae...
  45. ncbi [The genome sequence of soil bacterium Streptomyces]
    Haruo Ikeda
    Tanpakushitsu Kakusan Koso 47:1845-50. 2002
  46. ncbi Synthesis and biological evaluation of novel 4"-alkoxy avermectin derivatives
    Kenichiro Nagai
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University and The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
    Bioorg Med Chem Lett 14:4135-9. 2004
    ..Novel 4"-alkoxy avermectin derivatives were synthesized via rhodium carbenoid mediated O-H insertion reaction and tested for antiparasite activity against Artemia salina and Caenorhabditis elegans...
  47. ncbi In vitro and in vivo antimalarial activities of a carbohydrate antibiotic, prumycin, against drug-resistant strains of Plasmodia
    Kazuhiko Otoguro
    J Antibiot (Tokyo) 57:400-2. 2004
  48. ncbi Studies on terpenoids produced by actinomycetes: oxaloterpins A, B, C, D, and E, diterpenes from Streptomyces sp. KO-3988
    Keiichiro Motohashi
    Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo 156 8502, Japan
    J Nat Prod 70:1712-7. 2007
    ..The absolute configuration of oxaloterpin A was determined by the modified Mosher's method as 3 R, 5 S, 8 S, 10 R, 13 S...
  49. ncbi A gene cluster for biosynthesis of the sesquiterpenoid antibiotic pentalenolactone in Streptomyces avermitilis
    Charles N Tetzlaff
    Department of Chemistry, Brown University, Box H, Providence, Rhode Island 02912-9108, USA
    Biochemistry 45:6179-86. 2006
    ..Furthermore, a second GAPDH isozyme (gap2, SAV6296) has been expressed in E. coli and shown to be inactivated by pentalenolactone...
  50. ncbi Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis
    Zheng You
    Department of Chemistry, Brown University, Box H, Providence, Rhode Island 02912 9108, USA
    J Am Chem Soc 128:6566-7. 2006
    ..The steady-state kinetic parameters were kcat = 4.2 +/- 0.6 s-1 and Km (5) = 0.57 +/- 0.19 mM. 8 is a new intermediate in the conversion of the sesquiterpene pentalenene (3) to pentalenolactone (1)...
  51. ncbi Isolation and identification of three new 5-alkenyl-3,3(2H)-furanones from two streptomyces species using a genomic screening approach
    Arjun H Banskota
    Ecopia BioSciences Inc, 7290 Frederick Banting, Montreal, Quebec, H4S 2A1, Canada
    J Antibiot (Tokyo) 59:168-76. 2006
    ..All three new compounds were tested for their electron transport inhibitory activities. They had IC50 values of 1-4 microg/ml against Ascaris suum NADH-fumarate reductase and 1-12 microg/ml against bovine heart NADH oxidase...
  52. ncbi Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis
    Richard Quaderer
    Department of Chemistry, Brown University, Box H, Providence, Rhode Island 02912-9108, USA
    J Am Chem Soc 128:13036-7. 2006
    ..The steady-state kinetic parameters for the oxidation of pentalenene (3) to pentalen-13-ol (6) were kcat = 0.503 +/- 0.006 min-1 and Km = 3.33+/-0.62 muM for 3...
  53. ncbi Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase
    David E Cane
    Department of Chemistry, Brown University, Box H, Providence, Rhode Island 02912 9108, USA
    J Antibiot (Tokyo) 59:471-9. 2006
    ....
  54. ncbi Asymmetric total synthesis of (+)-K01-0509 B: determination of absolute configuration
    Satoshi Tsuchiya
    Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Minato-ku, Tokyo 108-8641, Japan
    Org Lett 8:5577-80. 2006
    ..These reactions allowed the cyclic guanidine and the adjacent hydroxy group to be assembled, facilitating the asymmetric total synthesis and determination of the absolute stereochemistry of K01-0509 B. [reaction: see text]...
  55. ncbi Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate
    Zheng You
    Department of Chemistry, Brown University, Box H, Providence, RI 02912 9108, USA
    Arch Biochem Biophys 459:233-40. 2007
    ..0 in the physiological pH range, while a significant activity enhancement was observed from pH 9.0 to 11.3. At pH 8.0, PtlF had a k(cat) of 0.65+/-0.03 s(-1), with a K(m) for 9 of 6.5+/-1.5 microM and K(m) for NAD(+) of 25+/-3 microM...
  56. ncbi Advances in drug discovery and biochemical studies
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Trends Parasitol 23:223-9. 2007
    ..Intensive studies of parasite energy metabolism, such as NADH-fumarate reductase systems and the synthetic pathways of nucleic acids and amino acids, also contribute to the identification of novel and unique drug targets...
  57. ncbi Hyper-inducible expression system for streptomycetes
    Sachio Herai
    Institute of Applied Biochemistry and Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan
    Proc Natl Acad Sci U S A 101:14031-5. 2004
    ..Furthermore, the system functioned in important streptomycete strains. Thus, the P(nitA)-NitR system should be a powerful tool for improving the productivity of various useful products in streptomycetes...
  58. ncbi Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis
    Zheng You
    Department of Chemistry, Brown University, Providence, Rhode Island 02912 9108, USA
    J Biol Chem 282:36552-60. 2007
    ....