Affiliation: The Nippon Dental University
Imai A, Yoshie S, Haga Tsujimura M, Nashida T, Shimomura H. Exocyst subunits are involved in isoproterenol-induced amylase release from rat parotid acinar cells. Eur J Oral Sci. 2012;120:123-31 pubmed publisher
..These results suggest that an exocyst complex of eight subunits is required for amylase release from parotid acinar cells. ..
Imai A, Tsujimura M, Yoshie S, Fukuda M. The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells. Biochem Biophys Res Commun. 2015;461:469-74 pubmed publisher
..Our results indicated that Rab33A is a novel component of IPR-stimulated amylase secretion from parotid acinar cells. ..
Imai A, Yoshie S, Nashida T, Fukuda M, Shimomura H. Redistribution of small GTP-binding protein, Rab27B, in rat parotid acinar cells after stimulation with isoproterenol. Eur J Oral Sci. 2009;117:224-30 pubmed publisher
Imai A, Nashida T, Shimomura H. mRNA expression of membrane-fusion-related proteins in rat parotid gland. Arch Oral Biol. 2001;46:955-62 pubmed
..mRNA expressions of many SNAREs and of the membrane-fusion-proteins suggest novel interactions for the regulation of salivary exocytosis. ..
Imai A, Nashida T, Yoshie S, Shimomura H. Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane. Arch Oral Biol. 2003;48:597-604 pubmed
..Many SNARE complexes were detected under non-stimulated/basic conditions in the parotid APM. Some of these complexes may have a role in exocytosis from parotid acinar cells. ..
Imai A, Nashida T, Shimomura H. Roles of Munc18-3 in amylase release from rat parotid acinar cells. Arch Biochem Biophys. 2004;422:175-82 pubmed
..The results indicate that Munc18-3 regulates exocytosis in the acinar cells for IPR-induced amylase release and that phosphorylation of Munc18-3 by PKA is not involved in the mechanism. ..
Imai A, Yoshie S, Nashida T, Shimomura H, Fukuda M. Functional involvement of Noc2, a Rab27 effector, in rat parotid acinar cells. Arch Biochem Biophys. 2006;455:127-35 pubmed
..Our results suggest that the Noc2/Rab27 complex is an important constituent of the early stages of IPR-stimulated amylase release. ..
Imai A, Yoshie S, Ishibashi K, Haga Tsujimura M, Nashida T, Shimomura H, et al
. EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells. J Biol Chem. 2011;286:33854-62 pubmed publisher
..Our findings indicated that EPI64 acted as a physiological Rab27-GAP that enhanced GTPase activity of Rab27 in response to IPR stimulation, and that this activity is required for IPR-induced amylase release. ..
Imai A, Ishida M, Fukuda M, Nashida T, Shimomura H. MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch Biochem Biophys. 2013;536:31-7 pubmed publisher
..Our findings indicated that MADD functions as a GEF for Rab27 in parotid acinar cells and that its GEF activity for Rab27, i.e., GDP/GTP cycling, is required for IPR-induced amylase release. ..