Tomohiko Ohta

Summary

Affiliation: St. Marianna University School of Medicine
Country: Japan

Publications

  1. ncbi request reprint [Breast and ovarian cancer susceptibility gene product BRCA1]
    Tomohiko Ohta
    Tanpakushitsu Kakusan Koso 51:1395-400. 2006
  2. doi request reprint HERC2 is an E3 ligase that targets BRCA1 for degradation
    WenWen Wu
    Division of Breast and Endocrine Surgery, Department of Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 70:6384-92. 2010
  3. doi request reprint The BRCA1 ubiquitin ligase and homologous recombination repair
    Tomohiko Ohta
    Department of Translational Oncology, St Marianna University Graduate School of Medicine, Kawasaki 216 8511, Japan
    FEBS Lett 585:2836-44. 2011
  4. pmc The ubiquitin E3 ligase activity of BRCA1 and its biological functions
    WenWen Wu
    Division of Breast and Endocrine Surgery, St, Marianna University School of Medicine, Kawasaki, 216 8511, Japan
    Cell Div 3:1. 2008
  5. ncbi request reprint Ubiquitin and breast cancer
    Tomohiko Ohta
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Oncogene 23:2079-88. 2004
  6. doi request reprint Contemplating chemosensitivity of basal-like breast cancer based on BRCA1 dysfunction
    Tomohiko Ohta
    Division of Breast and Endocrine Surgery, Department of Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Breast Cancer 16:268-74. 2009
  7. ncbi request reprint Down-regulation of BRCA1-BARD1 ubiquitin ligase by CDK2
    Ryosuke Hayami
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 65:6-10. 2005
  8. pmc Prediction of breast cancer sensitivity to neoadjuvant chemotherapy based on status of DNA damage repair proteins
    Hideki Asakawa
    Division of Breast and Endocrine Surgery, Department of Surgery, St, Marianna University School of Medicine, Kawasaki, Japan
    Breast Cancer Res 12:R17. 2010
  9. pmc Perturbation of DNA repair pathways by proteasome inhibitors corresponds to enhanced chemosensitivity of cells to DNA damage-inducing agents
    Takashi Takeshita
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Chemother Pharmacol 64:1039-46. 2009
  10. doi request reprint Recruitment of phosphorylated NPM1 to sites of DNA damage through RNF8-dependent ubiquitin conjugates
    Ayaka Koike
    Department of Translational Oncology, St Marianna University Graduate School of Medicine, Kawasaki, Japan
    Cancer Res 70:6746-56. 2010

Collaborators

Detail Information

Publications35

  1. ncbi request reprint [Breast and ovarian cancer susceptibility gene product BRCA1]
    Tomohiko Ohta
    Tanpakushitsu Kakusan Koso 51:1395-400. 2006
  2. doi request reprint HERC2 is an E3 ligase that targets BRCA1 for degradation
    WenWen Wu
    Division of Breast and Endocrine Surgery, Department of Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 70:6384-92. 2010
    ..The HERC2 expression in breast epithelial cells and breast carcinomas suggests that this mechanism may play a role in breast carcinogenesis...
  3. doi request reprint The BRCA1 ubiquitin ligase and homologous recombination repair
    Tomohiko Ohta
    Department of Translational Oncology, St Marianna University Graduate School of Medicine, Kawasaki 216 8511, Japan
    FEBS Lett 585:2836-44. 2011
    ..Here, we review recent advances in our understanding of BRCA1 function in HR and discuss possible roles of the activity...
  4. pmc The ubiquitin E3 ligase activity of BRCA1 and its biological functions
    WenWen Wu
    Division of Breast and Endocrine Surgery, St, Marianna University School of Medicine, Kawasaki, 216 8511, Japan
    Cell Div 3:1. 2008
    ..Elucidation of this mechanism in relation to cell survival is now much anticipated because it could be a key to predict chemosensitivity of basal-like breast cancer...
  5. ncbi request reprint Ubiquitin and breast cancer
    Tomohiko Ohta
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Oncogene 23:2079-88. 2004
    ..Understanding these pathways may provide some hints toward developing diagnostic tools and treatments for breast cancer patients...
  6. doi request reprint Contemplating chemosensitivity of basal-like breast cancer based on BRCA1 dysfunction
    Tomohiko Ohta
    Division of Breast and Endocrine Surgery, Department of Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Breast Cancer 16:268-74. 2009
    ..In individualized treatment of breast cancer, evaluation of the DNA repair capacity by the BRCA1 pathway may be an important issue when determining proper treatment of basal-like breast cancer...
  7. ncbi request reprint Down-regulation of BRCA1-BARD1 ubiquitin ligase by CDK2
    Ryosuke Hayami
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 65:6-10. 2005
    ..Recognizing the importance of cyclin E1 overexpression in breast cancer development, these results suggest a CDK2-BRCA1-NPM pathway that coordinately functions in cell growth and tumor progression pathways...
  8. pmc Prediction of breast cancer sensitivity to neoadjuvant chemotherapy based on status of DNA damage repair proteins
    Hideki Asakawa
    Division of Breast and Endocrine Surgery, Department of Surgery, St, Marianna University School of Medicine, Kawasaki, Japan
    Breast Cancer Res 12:R17. 2010
    ..Proteins required for this pathway can be detected as nuclear foci at sites of DNA damage when the pathway is intact. Here we investigate whether focus formation of repair proteins can predict chemosensitivity of breast cancer...
  9. pmc Perturbation of DNA repair pathways by proteasome inhibitors corresponds to enhanced chemosensitivity of cells to DNA damage-inducing agents
    Takashi Takeshita
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Chemother Pharmacol 64:1039-46. 2009
    ..We investigated the effect of proteasome inhibitors on the pathway after exposure to chemotherapeutic agents and examined its correlation with cytotoxicity...
  10. doi request reprint Recruitment of phosphorylated NPM1 to sites of DNA damage through RNF8-dependent ubiquitin conjugates
    Ayaka Koike
    Department of Translational Oncology, St Marianna University Graduate School of Medicine, Kawasaki, Japan
    Cancer Res 70:6746-56. 2010
    ..Collectively, our findings suggest that phosphorylated NPM1 is a novel component in DSB repair that is recruited by ubiquitin conjugates downstream of RNF8 and RNF168...
  11. ncbi request reprint BRCA1 ubiquitinates RPB8 in response to DNA damage
    WenWen Wu
    Division of Breast and Endocrine Surgery, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Cancer Res 67:951-8. 2007
    ..Our findings suggest that ubiquitination of a common subunit of RNA polymerases is a mechanism underlying BRCA1-dependent cell survival after DNA damage...
  12. ncbi request reprint Nucleophosmin/B23 is a candidate substrate for the BRCA1-BARD1 ubiquitin ligase
    Ko Sato
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    J Biol Chem 279:30919-22. 2004
    ..Given the many overlapped functions between NPM and BRCA1, we propose that NPM is a strong candidate as a substrate of the BRCA1-BARD1 ubiquitin ligase...
  13. doi request reprint BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity
    Hiroyuki Nishikawa
    Division of Breast and Endocrine Surgery, Institute of Advanced Medical Science, and Department of Radiology, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 69:111-9. 2009
    ..Together, these results suggest that BAP1 and BRCA1/BARD1 coordinately regulate ubiquitination during the DNA damage response and the cell cycle...
  14. pmc Cytoplasmic destruction of p53 by the endoplasmic reticulum-resident ubiquitin ligase 'Synoviolin'
    Satoshi Yamasaki
    Department of Genome Science, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan
    EMBO J 26:113-22. 2007
    ..Our results provide novel insights into p53 signaling mediated by Synoviolin...
  15. doi request reprint HERC2 Interacts with Claspin and regulates DNA origin firing and replication fork progression
    Naoki Izawa
    Department of Translational Oncology, Gastroenterology and Hepatology, Clinical Oncology, and Breast and Endocrine Surgery, Institute of Advanced Medical Science, St Marianna University Graduate School of Medicine, Kawasaki, Japan
    Cancer Res 71:5621-5. 2011
    ..These findings establish HERC2 as a critical function in DNA repair, checkpoint activation, and DNA replication...
  16. ncbi request reprint A truncated splice variant of human BARD1 that lacks the RING finger and ankyrin repeats
    Makiko Tsuzuki
    Division of Breast and Endocrine Surgery, Department of Surgery, St Marianna University School of Medicine, 2 16 1 Sugao, Miyamae ku, Kawasaki 216 8511, Japan
    Cancer Lett 233:108-16. 2006
    ..DeltaRIN does not interact with BRCA1, whereas it interacts with and colocalizes with CstF-50 to cytoplasmic dots. Hence, a deletion variant of BARD1 occurs in cells and may play a distinct role with CstF-50...
  17. ncbi request reprint Inhibition of caspase-9 activity and Apaf-1 expression in cisplatin-resistant head and neck squamous cell carcinoma cells
    Daisuke Kuwahara
    Department of Otolaryngology, St Marianna University School of Medicine, 2 16 1 Sugao, Miyamae ku, 216 8511, Kawasaki, Japan
    Auris Nasus Larynx 30:S85-8. 2003
    ..These results suggest that in a certain type of HNSCCs, the inhibition of Casp9 activity and Apaf-1 expression may represent a mechanism of acquired cisplatin resistance...
  18. ncbi request reprint Mass spectrometric and mutational analyses reveal Lys-6-linked polyubiquitin chains catalyzed by BRCA1-BARD1 ubiquitin ligase
    Hiroyuki Nishikawa
    Division of Breast and Endocrine Surgery, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    J Biol Chem 279:3916-24. 2004
    ..Hence, the results indicate that BRCA1-BARD1 mediates novel polyubiquitin chains that may be distinctly edited by 26 S proteasome from conventional Lys-48-linked polyubiquitin chains...
  19. doi request reprint Clinical efficacy and value of redistributed subclavian arterial infusion chemotherapy for locally advanced breast cancer
    Hiroshi Shimamoto
    Department of Radiology, St Marianna University School of Medicine, 2 16 1 Sugao, Miyamae ku, Kawasaki 216 8511, Japan
    Jpn J Radiol 29:236-43. 2011
    ..We have focused on the local response, quality of life (QOL), and complications. We have also investigated factors that influence the local response of RESAIC...
  20. ncbi request reprint [Predictive markers in breast cancer treatment--focusing on a recent topic "basal-like breast cancer"]
    Ayaka Koike
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine
    Nihon Rinsho 65:285-90. 2007
  21. pmc The UPS: a promising target for breast cancer treatment
    Ko Sato
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, 216 8511, Japan
    BMC Biochem 9:S2. 2008
    ..PUBLICATION HISTORY : Republished from Current BioData's Targeted Proteins database (TPdb; http://www.targetedproteinsdb.com)...
  22. ncbi request reprint Putative tumor suppressor EDD interacts with and up-regulates APC
    Ryuichi Ohshima
    Division of Gastroenterological Surgery, Department of Surgery, St Marianna University School of Medicine, Kawasaki 216 8511, Japan
    Genes Cells 12:1339-45. 2007
    ..Thus, through protein-protein interaction, EDD stabilizes APC and up-regulates APC's function to inhibit beta-catenin, suggesting that EDD could act as a colorectal tumor suppressor...
  23. ncbi request reprint [Breast-conserving surgery: quadrantectomy]
    Hisanori Kawamoto
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Nihon Geka Gakkai Zasshi 103:811-5. 2002
    ..It is important to determine the surgical technique that compromises between local curability and cosmetic results for each patient with breast cancer...
  24. doi request reprint Radiologic-pathological correlation of punctate hyperechoic foci by ultrasound in stereotactic vacuum-assisted breast biopsy samples
    Hiroko Okazaki
    Department of Radiology, St Marianna University School of Medicine, Miyamae ku, Kawasaki, Japan
    Jpn J Radiol 27:438-43. 2009
    ..To correlate punctate hyperechoic foci (PHF) on ultrasound (US) with microcalcifications detected by mammography (MMG) and at histopathology...
  25. ncbi request reprint Sensitization of head and neck squamous cell carcinoma cells to Fas-mediated apoptosis by the inhibition of Bcl-X(L) expression
    Yoshihiro Otani
    Department of Otolaryngology, St Marianna University School of Medicine, 2 16 1 Sugao, Miyamae ku, 216 8511, Kawasaki, Japan
    Auris Nasus Larynx 30:S79-84. 2003
    ..In Fas-sensitive cells, Fas-mediated apoptosis is observed when anti-Fas antibody is bound to Fas. Bcl-2 and Bcl-X(L) are representative anti-apoptosis proteins reported to be capable of suppressing Fas-mediated apoptosis...
  26. ncbi request reprint [Breast cancer]
    Mamoru Fukuda
    Division of Breast and Endocrine Surgery, St Marianna University School of Medicine
    Gan To Kagaku Ryoho 29:1900-6. 2002
    ..These controversies show the importance of assessment of screening mammography effectiveness in Japan...
  27. ncbi request reprint Essential role of synoviolin in embryogenesis
    Naoko Yagishita
    Department of Genome Science, Institute of Medical Science, St Marianna University School of Medicine, 2 16 1 Sugao Miyamae ku, Kawasaki, Kanagawa 216 8512, Japan
    J Biol Chem 280:7909-16. 2005
    ..Our findings emphasized the indispensable role of the Synoviolin in embryogenesis...
  28. ncbi request reprint Targeted substrate degradation by an engineered double RING ubiquitin ligase
    Daisuke Oyake
    Department of Surgery, St Marianna University School of Medicine, 2 16 1 Sugao, Miyamae ku, Kawaski 216 8511, Japan
    Biochem Biophys Res Commun 295:370-5. 2002
    ..By redesigning the substrate recognition site, expression of engineered double RING ubiquitin ligases may provide a useful tool for removing many different gene products at the protein level...
  29. pmc Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex
    Peter S Brzovic
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:5646-51. 2003
    ..Thus, binding alone is not sufficient for BRCA1-dependent Ub-ligase activity...
  30. ncbi request reprint A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair
    Nobuko Matsushita
    Department of Immunology and Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama 701 0192, Japan
    Mol Cell 19:841-7. 2005
    ..These results define functional consequences of FancD2 monoubiquitination and reveal previously hidden functions for the FA protein core complex...
  31. ncbi request reprint Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage
    Jian Hu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Nat Cell Biol 6:1003-9. 2004
    ..We suggest that DDB1 targets CDT1 for ubiquitination by a CUL4A-dependent ubiquitin ligase, CDL4A(DDB1), in response to UV irradiation...
  32. ncbi request reprint Involvement of kinesin family member 2C/mitotic centromere-associated kinesin overexpression in mammary carcinogenesis
    Arata Shimo
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cancer Sci 99:62-70. 2008
    ..These findings suggest that overexpression of KIF2C/MCAK might be involved in breast carcinogenesis and is a promising therapeutic target for breast cancers...
  33. ncbi request reprint [Genetic abnormalities in ovarian tumors]
    Kazushige Kiguchi
    Division of Obstetrics and Gynecology, St Marianna University School of Medicine
    Nihon Rinsho 62:449-53. 2004
  34. ncbi request reprint Elevated expression of protein regulator of cytokinesis 1, involved in the growth of breast cancer cells
    Arata Shimo
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cancer Sci 98:174-81. 2007
    ..These findings suggest the involvement of a PRC1-KIF2C/MCAK complex in breast tumorigenesis, and this complex should be a promising target for the development of novel treatments for breast cancer...
  35. ncbi request reprint Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins
    Manabu Furukawa
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 277:15758-65. 2002
    ..We suggest that RING-E2, rather than cullin-RING, constitutes the catalytic core of the ubiquitin ligase and that one major function of the cullin subunit is to assemble the RING-E2 catalytic core and substrates together...