Kazuyuki Nakajima

Summary

Affiliation: Soka University
Country: Japan

Publications

  1. ncbi request reprint Ceramide-enhanced urokinase-type plasminogen activator (uPA) release is mediated by protein kinase C in cultured microglia
    K Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo, Japan
    Glia 32:226-33. 2000
  2. ncbi request reprint Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method
    K Nakajima
    Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo, Japan
    Biomaterials 22:1213-23. 2001
  3. doi request reprint Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro
    Kazuyuki Nakajima
    Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo 192 8577, Japan Electronic address
    Neurochem Int 102:1-12. 2016
  4. doi request reprint Up-regulation of glutamine synthesis in microglia activated with endotoxin
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo 192 8577, Japan Electronic address
    Neurosci Lett 591:99-104. 2015
  5. ncbi request reprint Microglia: neuroprotective and neurotrophic cells in the central nervous system
    Kazuyuki Nakajima
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Curr Drug Targets Cardiovasc Haematol Disord 4:65-84. 2004
  6. ncbi request reprint Protein kinase C alpha requirement in the activation of p38 mitogen-activated protein kinase, which is linked to the induction of tumor necrosis factor alpha in lipopolysaccharide-stimulated microglia
    Kazuyuki Nakajima
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurochem Int 44:205-14. 2004
  7. ncbi request reprint Suppression of lipopolysaccharide-dependent tumor necrosis factor alpha induction in rat microglia, in which protein kinase C alpha is down-regulated
    Kazuyuki Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 343:33-6. 2003
  8. ncbi request reprint Enhancement of urokinase-type plasminogen activator (uPA) secretion, but not that of substrate plasminogen (PGn), by rat microglia stimulated with neuronal conditioned medium
    Kazuyuki Nakajima
    Neurobiology Laboratory, Department of Bioinformatics, Faculty of Engineering, Soka University, 4 1 1 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 378:13-7. 2005
  9. ncbi request reprint Activation of microglia with lipopolysaccharide leads to the prolonged decrease of conventional protein kinase C activity
    Kazuyuki Nakajima
    Neurochemistry Division, Institute of Life Science, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Brain Res Mol Brain Res 110:92-9. 2003
  10. ncbi request reprint Differential suppression of endotoxin-inducible inflammatory cytokines by nuclear factor kappa B (NFkappaB) inhibitor in rat microglia
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 401:199-202. 2006

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Ceramide-enhanced urokinase-type plasminogen activator (uPA) release is mediated by protein kinase C in cultured microglia
    K Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo, Japan
    Glia 32:226-33. 2000
    ..The findings suggest that uPA release from microglia is regulated by a mechanism in which PKC-delta and/or -epsilon are activated and further signals are transduced subsequently...
  2. ncbi request reprint Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method
    K Nakajima
    Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo, Japan
    Biomaterials 22:1213-23. 2001
    ..This new technology exhibits utility for the preparation of cell sources required for cell transplantation as well as microglial function analysis...
  3. doi request reprint Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro
    Kazuyuki Nakajima
    Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo 192 8577, Japan Electronic address
    Neurochem Int 102:1-12. 2016
    ..Taken together, these results demonstrate that axoFN-microglia express functional GLT-1 and GS proteins, and are strongly suggested to serve as Glu scavengers in vivo...
  4. doi request reprint Up-regulation of glutamine synthesis in microglia activated with endotoxin
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo 192 8577, Japan Electronic address
    Neurosci Lett 591:99-104. 2015
    ..Microglia were thus, strongly suggested to metabolize Glu to Gln via GS activity when activated in the inflammatory/pathological state of the nervous system. ..
  5. ncbi request reprint Microglia: neuroprotective and neurotrophic cells in the central nervous system
    Kazuyuki Nakajima
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Curr Drug Targets Cardiovasc Haematol Disord 4:65-84. 2004
    ..In addition, the mechanism by which neurotrophic microglia become oriented to a neurotoxic state is discussed...
  6. ncbi request reprint Protein kinase C alpha requirement in the activation of p38 mitogen-activated protein kinase, which is linked to the induction of tumor necrosis factor alpha in lipopolysaccharide-stimulated microglia
    Kazuyuki Nakajima
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurochem Int 44:205-14. 2004
    ....
  7. ncbi request reprint Suppression of lipopolysaccharide-dependent tumor necrosis factor alpha induction in rat microglia, in which protein kinase C alpha is down-regulated
    Kazuyuki Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 343:33-6. 2003
    ..Taken together, the present study demonstrated that PKC alpha is a requisite PKC isoform in the signaling cascade of TNF alpha induction in LPS-stimulated microglia...
  8. ncbi request reprint Enhancement of urokinase-type plasminogen activator (uPA) secretion, but not that of substrate plasminogen (PGn), by rat microglia stimulated with neuronal conditioned medium
    Kazuyuki Nakajima
    Neurobiology Laboratory, Department of Bioinformatics, Faculty of Engineering, Soka University, 4 1 1 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 378:13-7. 2005
    ..In summary, Neuron-derived soluble molecules appear to stimulate microglia to enhance the production/release of uPA, but not that of PGn, by a mechanism independent of the activation reaction by IFN-gamma or LPS...
  9. ncbi request reprint Activation of microglia with lipopolysaccharide leads to the prolonged decrease of conventional protein kinase C activity
    Kazuyuki Nakajima
    Neurochemistry Division, Institute of Life Science, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Brain Res Mol Brain Res 110:92-9. 2003
    ..Therefore, it is suggested that LPS-stimulated microglia require priming by PKC activation for the induction of harmful factors, while only a part (30%) of original PKC activity is sufficient for durable microglial activation...
  10. ncbi request reprint Differential suppression of endotoxin-inducible inflammatory cytokines by nuclear factor kappa B (NFkappaB) inhibitor in rat microglia
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 401:199-202. 2006
    ..Taken together, these results suggest that the induction of IL-1beta and TNFalpha in endotoxin-stimulated microglia is differentially regulated at the level of NFkappaB activation...
  11. ncbi request reprint In vitro proliferation of axotomized rat facial nucleus-derived activated microglia in an autocrine fashion
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan
    J Neurosci Res 84:348-59. 2006
    ..In summary, the microglia isolated from the axFN were found to be proliferative in an autocrine fashion and to have some cellular properties in common with those observed in vivo...
  12. ncbi request reprint Ceramide activates microglia to enhance the production/secretion of brain-derived neurotrophic factor (BDNF) without induction of deleterious factors in vitro
    Kazuyuki Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo, Japan
    J Neurochem 80:697-705. 2002
    ..These results indicate that ceramide-induced BDNF release in microglia is mediated by a signaling pathway associated with PKCdelta and/or epsilon, but not with activation of MAPKs, CREB and NFkappaB...
  13. ncbi request reprint Neurotrophin secretion from cultured microglia
    K Nakajima
    Institute of Life Science, Soka University, 1 236, Tangi machi, Hachioji, Tokyo 192 8577
    J Neurosci Res 65:322-31. 2001
    ..Taken together, these findings suggest that the secretion of BDNF from microglia is regulated through PKCalpha-associated signal transduction mechanism...
  14. ncbi request reprint Neuronal regulation by which microglia enhance the production of neurotrophic factors for GABAergic, catecholaminergic, and cholinergic neurons
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Neurochem Int 50:807-20. 2007
    ..Taken together, neuronal stimulus was found to activate microglia to produce more neurotrophic factors as above, thereby changing microglia into more neurotrophic cells...
  15. ncbi request reprint Ability of rat microglia to uptake extracellular glutamate
    K Nakajima
    Institute of Life Science, Soka University, 1 236 Tangi machi, Hachioji, 192 8577, Tokyo, Japan
    Neurosci Lett 307:171-4. 2001
    ..All together, these results indicate that microglia can act as glutamate scavengers in vivo by expressing the glutamate transporter GLT-1...
  16. doi request reprint Neuronal stimulation leading to upregulation of glutamate transporter-1 (GLT-1) in rat microglia in vitro
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 436:331-4. 2008
    ..Altogether, neuronal stimulus was found to promote the uptake of Glu in microglia, probably due to the increased levels of GLT-1...
  17. doi request reprint Close association of p38 and JNK with plasminogen-dependent upregulation of PAI-1 in rat astrocytes in vitro
    Kazuyuki Nakajima
    Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurosci Lett 471:66-9. 2010
    ..In conclusion, p38 and JNK were shown to be the major MAPKs involved in the signaling cascade leading to PAI-1 induction in astrocytes stimulated with PLGn...
  18. ncbi request reprint Axotomy-dependent urokinase induction in the rat facial nucleus: possible stimulation of microglia by neurons
    Kazuyuki Nakajima
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Neurochem Int 46:107-16. 2005
    ..Thus, uPA induction in the axotomized facial nucleus may be explained by a neuronal stimulus leading to uPA induction in microglia...
  19. ncbi request reprint Nonparticipation of nuclear factor kappa B (NFkappaB) in the signaling cascade of c-Jun N-terminal kinase (JNK)- and p38 mitogen-activated protein kinase (p38MAPK)-dependent tumor necrosis factor alpha (TNFalpha) induction in lipopolysaccharide (LPS)-stim
    Miyuki Uesugi
    Neurobiology Lab, Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192 8577, Japan
    Brain Res 1073:48-59. 2006
    ..These results suggest that both MKK3/6-p38MAPK and MKK4-JNK pathways are important signaling cascades leading to the induction of TNFalpha in LPS-stimulated microglia, but that NFkappaB itself is not required for this induction...
  20. doi request reprint Characteristic response of astrocytes to plasminogen/plasmin to upregulate transforming growth factor beta 3 (TGFbeta3) production/secretion through proteinase-activated receptor-1 (PAR-1) and the downstream phosphatidylinositol 3-kinase (PI3K)-Akt/PKB si
    Shyuichi Maeda
    Department of Bioinformatics, Faculty of Engineering, Soka University, 1 236 Tangi machi, Hachioji, Tokyo 192 8577, Japan
    Brain Res 1305:1-13. 2009
    ..We have therefore demonstrated that PLGn/plasmin, probably plasmin, facilitates the production/secretion of TGFbeta3 in astrocytes through both PAR-1 and the subsequent signaling cascade including PI3K and Akt...
  21. doi request reprint Activation of microglia by endotoxin suppresses the secretion of glial cell line-derived neurotrophic factor (GDNF) through the action of protein kinase C alpha (PKCalpha) and mitogen-activated protein kinases (MAPKS)
    Yuichi Matsushita
    Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan
    J Neurosci Res 86:1959-71. 2008
    ....
  22. doi request reprint Macrophage-colony stimulating factor as an inducer of microglial proliferation in axotomized rat facial nucleus
    Shinichi Yamamoto
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan
    J Neurochem 115:1057-67. 2010
    ..These results demonstrate that up-regulated M-CSF triggers the induction of cFms in microglia and causes the microglia to proliferate in the axotFN...
  23. doi request reprint Role of cell cycle-associated proteins in microglial proliferation in the axotomized rat facial nucleus
    Shinichi Yamamoto
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan
    Glia 60:570-81. 2012
    ....
  24. doi request reprint Superoxide anion contributes to the induction of tumor necrosis factor alpha (TNFα) through activation of the MKK3/6-p38 MAPK cascade in rat microglia
    Yukihisa Yoshino
    Department of Bioinformatics, Faculty of Engineering, Soka University, Japan
    Brain Res 1422:1-12. 2011
    ..Taken together, these results showed that O(2)(-) is an important signaling molecule for activating the MKK3/6-p38 cascade, which is requisite for inducing TNFα in microglia...
  25. ncbi request reprint Spatial and temporal aspects of Ca2+ signaling mediated by P2Y receptors in cultured rat hippocampal astrocytes
    Schuichi Koizumi
    Division of Pharmacology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya, 158, Tokyo, Japan
    Life Sci 72:431-42. 2002
    ..It should be noted that these Ca2+ signals and their modulation by Zn2+ could occur in the hippocampus in situ since both ATP and Zn2+ are rich in the hippocampus and could be released by excitatory stimulation...
  26. ncbi request reprint Cell transplantation to the brain with microglia labeled by neuropathogenic retroviral vector system
    Rihito Watanabe
    Institute of Life Science, Soka University, Hachioji, Tokyo, Japan
    Cell Transplant 11:471-3. 2002
    ..None of the rats showed pathological changes in the whole body investigated, including the central nervous system, 4 weeks after transplantation of the labeled microglial cells...
  27. ncbi request reprint Alpha1,3-fucosyltransferase IX (Fut9) determines Lewis X expression in brain
    Shoko Nishihara
    Division of Cell Biology, Institute of Life Science, Soka University, Tangi cho, Hachioji, Tokyo 192 8577, Japan
    Glycobiology 13:445-55. 2003
    ..Immunohistochemistry with anti-Fut9 monoclonal antibody revealed the distribution of the Lex structure. These results showed that Fut9 is the most responsible enzyme for the synthesis of Lex in brain...
  28. ncbi request reprint Early axonal and glial pathology in fetal sheep brains with leukomalacia induced by repeated umbilical cord occlusion
    J Ohyu
    Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan
    Brain Dev 21:248-52. 1999
    ..These findings suggest the glial reaction of cytokines and free radicals induced by fetal hypoxia may contribute to the occurrence of brain injury...
  29. doi request reprint Involvement of nitric oxide in the induction of interleukin-1 beta in microglia
    Kenji Sudo
    Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Tokyo 192 8577, Japan
    Brain Res 1625:121-34. 2015
    ..Taken together, these results indicate that NO is an important signaling molecule for the ERK/JNK and NFκB activations, which are requisite to the induction of IL-1β in microglia...
  30. doi request reprint Accumulation of glycogen in axotomized adult rat facial motoneurons
    Yosuke Takezawa
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan
    J Neurosci Res 93:913-21. 2015
    ....
  31. doi request reprint Transient down-regulation and restoration of glycogen synthase levels in axotomized rat facial motoneurons
    Yosuke Takezawa
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo 192 8577, Japan
    Brain Res 1586:34-45. 2014
    ..Taken together, these results demonstrated that the injured facial motoneurons transiently reduced glycogen synthase levels at around 1-2 weeks post-insult, but restored the levels at 4-5 weeks post-insult. ..
  32. doi request reprint Functional down-regulation of axotomized rat facial motoneurons
    Toshihumi Ichimiya
    Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo 192 8577, Japan
    Brain Res 1507:35-44. 2013
    ....
  33. ncbi request reprint Microglia: activation and their significance in the central nervous system
    K Nakajima
    Institute of Life Science, Soka University, Hachioji, Tokyo
    J Biochem 130:169-75. 2001
    ....
  34. ncbi request reprint Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex
    H Tabata
    Department of Molecular Neurobiology, Institute of DNA Medicine, Jikei University School of Medicine, Minato-ku, 105-8461, Tokyo, Japan
    Neuroscience 103:865-72. 2001
    ..Thus, this system is much easier and more efficient than virus-mediated gene transfer, and is useful for gain-of-function analysis of neural cell fate determination, migration, positioning and axon path-finding in mouse embryos...
  35. ncbi request reprint Replication patterns of repetitive DNA sequences on the W chromosome are altered during development of the chick embryo
    S Tanaka
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo, Japan
    Exp Cell Res 223:233-41. 1996
    ..The data suggest that a link might be operative between replication patterns of genes and the developmental program...
  36. doi request reprint Microglia and astroglia prevent oxidative stress-induced neuronal cell death: implications for aceruloplasminemia
    Satoru Oshiro
    Division of Molecular and Cellular Biochemistry, Department of Health Science, Faculty of Sports and Health Sciences, Daito Bunka University, 560 Iwadono, Higashi Matsuyama, Saitama 355 8501, Japan
    Biochim Biophys Acta 1782:109-17. 2008
    ..Therefore, the different mechanisms of neuronal cell protection by MG and AS may explain the pathophysiological observations in the brains of patient with aceruloplasminemia...
  37. ncbi request reprint Expression of the mRNA and protein of serine racemase in primary cultures of rat neurons
    Masanobu Yoshikawa
    Department of Clinical Pharmacology, Tokai University School of Medicine, Isehara, Kanagawa 259 1143, Japan
    Eur J Pharmacol 548:74-6. 2006
    ..These data are the first to demonstrate that a substantial amount of serine racemase exists in the cultured neurons...
  38. ncbi request reprint Cloning and characterization of a new human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-GalNAc-T13, that is specifically expressed in neurons and synthesizes GalNAc alpha-serine/threonine antigen
    Yan Zhang
    Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, Open Space Laboratory, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    J Biol Chem 278:573-84. 2003
    ..pp-GalNAc-T13 would be a major enzyme responsible for the synthesis of O-glycan and specifically the Tn antigen epitope in neurons...