Shiseido Research Center

..Our results indicate that TRPM8 or a closely related protein in epidermal keratinocytes plays a role in epidermal permeability barrier homoeostasis and epidermal proliferation after barrier insult...

..This method is thought to be widely and conveniently applicable for determining the level of ubiquinones because of its high selectivity for ubiquinone detection...

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..The ATP concentration also increased immediately after keratinocytes were exposed to air. We hypothesize that ATP is secreted from keratinocytes on exposure to air, and induces an increase of intracellular calcium concentration...

..These results suggest that NO plays an important signaling role in cutaneous barrier homeostasis and in epidermal hyperplasia induced by barrier disruption...

..8%. From this, it is concluded that this method could be used to predict the acute systemic toxicity potential of chemicals in rodents...

..In this study, we applied Near infrared spectroscopy (NIR)-based prediction of water content of the cut nail plate to in vivo measurement to obtain information about the seasonal change of water in the fingernail...

..Carbonylation of CEs did not depend on their maturity, as evaluated by loss of involucrin antigenicity during maturation process, suggesting that CEs are carbonylated regardless of their maturation stage...

..Although matrix metalloproteinases (MMPs) are believed to be involved in the BM damage, there is no good in vitro model for examining BM damage by MMPs or for exploring methods to protect the BM...

..Finally, mathematical analysis was done to evaluate the effect of BO-2, and it was shown that BO-2 improved the LID solubility in stratum corneum lipids to efficiently enhance the LID permeation through skin...

..These analyses will contribute to elucidating the mechanisms of hair cycle regulation and should lead to the identification of novel molecular targets for hair growth and/or depilation agents...

..We found that xylitol (X) and farnesol (F) synergistically inhibited biofilm formation by SA and dissolved biofilm formed in vivo (Part 1)...

..Staphylococcus epidermidis (SE) is a major constituent of skin microflora on healthy human skin, and provides protection against the growth of pathogenic bacteria...

..These in vivo studies confirmed that vascular change is an important factor for the development of the immediate pigment darkening and delayed tanning...

..The values obtained showed an excellent correlation with in vivo SPF values, even for high-SPF sunscreens exceeding SPF 50. This method should be suitable as an in vitro SPF testing method for regulatory purposes...

..As a hallmark of oxidatively damaged protein, protein carbonyl, including acrolein-protein adduct, has been observed in the skin. However, the influence of protein carbonylation on the stratum corneum (SC) has not yet been clarified...

..The results show this method can be used to measure cis- and trans-UCA levels in vivo...

..These results suggest that differentiated keratinocytes are sensitive to external pressure and that TRP might be involved in the mechanism of their response...

..Our results demonstrate that grapefruit oil efficiently inhibits adipogenesis in cultured subcutaneous preadipocytes and adipocytes...

..There was a significant correlation between work function of each metal and barrier recovery rate. These results suggest that electron donation from outside accelerated the skin barrier recovery...

..These results are consistent with the idea that NO generated by epidermal nNOS has a significant role in the cutaneous circulatory response to mechanical stimulation...

..The aim of this study was to investigate the mechanism of facial sagging by examining the relationship of sagging severity to changes of skin elasticity, fat mass and facial muscle function at the cheek...

..The results of the present study clearly demonstrate that some ceramide orally administered is distributed gradually in the dermis after gastrointestinal absorption, followed by transfer from the dermis to the epidermis...

..It is crucial to establish an accurate method for measuring skin pigmentation in cosmetic science and clinical dermatology. Here, we report a non-invasive precise method for measuring skin melanin content...

..We conclude that, until puberty, most functional characteristics of the skin of children remain distinct from those of adults...

..Thus, PEPTME (38/10) was considered to be most suited for preparing highly concentrated microemulsions...

..Unsaturated fatty acids from sebum affect calcium dynamics in epidermal keratinocytes, disrupt the barrier function and induce abnormal keratinization. However, the mechanisms of these effects have not been clarified...

..The phase diagram analysis suggested that EPDME could form emulsified particles having lamellar structure on the surface of the particle with hydrophilic sterol surfactant in polar oils...

..acnes existing as normal resident flora plays an important role in the exacerbation of acne vulgaris as well as in possible induction of corticosteroid-induced acne or in that of rosacea-like dermatitis...

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..In conclusion, RCM successfully evidenced the difference in melanin distribution between the pigmented animals and humans, and the UV-induced pigmentation process in vivo as well...

..The objective here is to measure skin surface friction using these devices and examine the correlation with other physiological parameters in order to evaluate the potential of physical measurement of tactile sensation...

..This chemotactic signal between dermal papillae and melanocytes may control the localization and migration of hair melanocytes in vivo...

..997. Recoveries from spiked serum and urine samples, estimated by the external standard method, were between 94.8 and 117.6% with a relative standard deviation (RSD) ranging from 2.1 to 18%...

..RT-PCR assay of cultured human keratinocyte also showed expression of VR1 mRNA. These results suggest the existence of VR1-like protein in epidermal keratinocytes of human skin...

..However, 4GBE43 at 10(-4)-10(-5) M completely inhibited peroxidation, showing it to be more effective than alpha-tocopherol. These results suggest that 4GBE43 and 2BBE43 act as antioxidants by quenching the lipid peroxyl radical...

..These results suggest that the ion flux in the nucleated layer of the epidermis induce the skin surface potential and it is influenced by environmental and neuroendocrinological factors...

..The results indicate that 2-nonenal is generated by the oxidative degradation of omega7 unsaturated fatty acids, and suggest that 2-nonenal may be involved in the age-related change of body odor...

..In conclusion, both the histamine H1 and H2 receptors in the epidermis are involved in skin barrier function and the cutaneous condition of epidermal hyperplasia...

..Regulation of protease activity or ionic balance in the epidermis can accelerate barrier repair after injury. Improvement of the stratum corneum homeostasis can ameliorate skin damage induced by barrier disruption in a dry environment...

..These results suggest that the effects of these metal ions are different depending on the counter ion and/or the method of application...

..In conclusion, the MC1R promoter shares the characteristics of many other GPCR promoters. These characteristics include GC-rich sequence, lack of a TATA box, and binding of SP-1...

..These animal models could be useful for objectively quantifying the influence of stress on the cutaneous function...

..Together with a recent report that anti-OPN antibodies disrupt kidney morphogenesis, our results suggest that interactions between OPN and integrin alpha8 beta1 may help regulate kidney development and other morphogenetic processes...

..We have examined the effect of low humidity on healthy human skin by using noninvasive measurement devices...

..The inducing effects of chemicals on the two phenotypical alterations were the same. These results suggest that these test systems can be used to predict contact-sensitizing ability of chemicals as an in vitro sensitization assay...

..These results suggest that the gamma-aminobutyric acid (A)-like receptor is associated with skin barrier homeostasis and regulation of the receptor clinically effective for barrier dysfunctional or epidermal hyperproliferative diseases...

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..Application of a chloride-exchange resin delayed barrier recovery. Topical application of ionic polymers influenced skin barrier homeostasis...

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..3-20%. The method was successfully applied for the endogenous saliva MLT monitoring of two healthy subjects...

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..These results obviously suggest that the maturation of CEs and disappearance of nuclei are differentially regulated in the epidermis...

..A variety of skin diseases, such as atopic dermatitis, senile xerosis or psoriasis tends to worsen during the dry winter season. These skin diseases are also characterized by itch sensation...

..These results suggest that, like the nervous system, influx of calcium and chloride ions into epidermal keratinocytes through ionotropic receptors plays a crucial role in cutaneous barrier homeostasis...

..Stress exacerbates some skin diseases. We have reported that inhalation of certain odorants modulates immune reactions in the skin...

..The results suggest that the newly-formed cartilaginous nodules were due to interstitial proliferation of chondrocytes...

..These results suggest that adrenocorticotropic hormone is at least partly responsible for the sensitivity of the pigmentary response after ultraviolet irradiation under stressful conditions...

..These results suggest that the beta2-adrenergic receptor is specifically associated with skin barrier homeostasis...

..An external negative electric field accelerated the skin barrier recovery. These new methods to improve skin barrier homeostasis could be useful strategies to solve skin problems...

..The SCD is a key enzyme in fatty acid biosynthesis and its expression is controlled by temperature, nutritional conditions and hormones. This is the first report that the mRNA expression of stearyl CoA is regulated by androgens...