Research Topics
Genomes and GenesSpecies | Nobuo OkumuraSummaryAffiliation: Shinshu University Country: Japan Publications
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Publications
Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit--LD-B Matsumoto I and II (LD-B W323R)N Okumura
Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
Clin Chim Acta 287:163-71. 1999- B:b interactions are essential for polymerization of variant fibrinogens with impaired holes 'a'N Okumura
Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Japan
J Thromb Haemost 5:2352-9. 2007..The role of A:a interactions is well established, but the roles of knob:hole interactions A:b, B:b or B:a remain ambiguous... - A novel variant fibrinogen, deletion of Bbeta111Ser in coiled-coil region, affecting fibrin lateral aggregationNobuo Okumura
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Japan
Clin Chim Acta 365:160-7. 2006..Functional fibrinogen concentration of a male infant showed <0.50 g/l and we speculated this patient as a dysfibrinogenemia or hypofibrinogenemia... - Evidence that heterodimers exist in the fibrinogen Matsumoto II (gamma308N-->K) proband and participate in fibrin fiber formationNobuo Okumura
Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
Thromb Res 107:157-62. 2002.... 
Citrullinated fibrinogen shows defects in FPA and FPB release and fibrin polymerization catalyzed by thrombinNobuo Okumura
Department of Biomedical Laboratory Sciences, Laboratory of Clinical Chemistry, School of Health Sciences, Shinshu University, Japan
Clin Chim Acta 401:119-23. 2009....- Hypofibrinogenemia associated with a heterozygous C-->T nucleotide substitution at position -1138 BP of the 5'-flanking region of the fibrinogen A alpha-chain geneN Okumura
Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
Ann N Y Acad Sci 936:526-30. 2001..These results suggest that the region adjacent to the -1138C bp of the 5'-flanking region of the fibrinogen A alpha gene is one of the most crucial sites for the transcription of the fibrinogen A alpha gene... - Substitution of the gamma-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linkingNobuo Okumura
Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
Blood 103:4157-63. 2004..Together, our results show substitution of Asn308 with a hydrophobic residue altered neither polymer formation nor polymer structure at physiologic calcium concentrations, whereas substitution with lysine altered both... - Difference in electrophoretic mobility and plasmic digestion profile between four recombinant fibrinogens, gamma 308K, gamma 308I, gamma 308A, and wild type (gamma 308N)N Okumura
Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
Electrophoresis 21:2309-15. 2000..In conclusion, a substitution at residue gamma308Asn may cause a conformational change in the gammachain of fragment D affecting polymerization and plasmin cleavage... - A C-terminal amino acid substitution in the gamma-chain caused by a novel heterozygous frameshift mutation (Fibrinogen Matsumoto VII) results in hypofibrinogenaemiaNoriko Fujihara
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Thromb Haemost 104:213-23. 2010.... - In vitro expression demonstrates impaired secretion of the gammaAsn319, Asp320 deletion variant fibrinogenSatomi Kani
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, 390 8621, Japan
Thromb Haemost 94:53-9. 2005..These results suggest that the reduced level of gammaDelta319,320 fibrinogen in the plasma of the Otsu patient arises from modestly impaired secretion of this variant fibrinogen... - Recombinant variant fibrinogens substituted at residues gamma326Cys and gamma339Cys demonstrated markedly impaired secretion of assembled fibrinogenAyumi Haneishi
Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan
Thromb Res 124:368-72. 2009.... - In vitro transcription of compound heterozygous hypofibrinogenemia Matsumoto IX; first identification of FGB IVS6 deletion of 4 nucleotides and FGG IVS3-2A>G causing abnormal RNA splicingFumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, 390 8621, Japan
Clin Chim Acta 411:1325-9. 2010..To examine the transcription of mRNA from the M IX gene, we cloned the wild-type and mutant genes into expression vectors... - Analysis of fibrinogen variants at gamma387Ile shows that the side chain of gamma387 and the tertiary structure of the gammaC-terminal tail are important not only for assembly and secretion of fibrinogen but also for lateral aggregation of protofibrils anSatomi Kani
Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
Blood 108:1887-94. 2006..Interestingly, our results clearly indicate that the conformations critical for these 2 functions are different from each other... - Molecular analysis of afibrinogenemic mutations caused by a homozygous FGA1238 bp deletion, and a compound heterozygous FGA1238 bp deletion and novel FGA c.54+3A>C substitutionYuka Takezawa
Department of Health and Medical Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Japan
Int J Hematol 96:39-46. 2012..Molecular analyses of both genetic variants suggest that the lack of a mature Aα-chain, impaired assembly, and/or secretion of the fibrinogen molecule may lead to afibrinogenemia... - In vitro expression of beta-thalassaemia gene (IVS1-1G>C) reveals complete inactivation of the normal 5' splice site and alternative aberrant RNA splicingNoriko Fujihara
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Ann Clin Biochem 44:573-8. 2007..Further studies will be required to clarify the molecular mechanism that results in severe reduction in the mutant mRNA level in vivo... - Application of allele-specific quantitative PCR using genomic DNA to monitor minimal residual disease based on mutant gene levels following allogeneic hematopoietic stem cell transplantation in patients with hematological malignancies: comparison of mutanChiaki Taira
Department of Laboratory Medicine, Shinshu University Hospital, Asahi, Matsumoto, Japan
Clin Chim Acta 413:516-9. 2012.... - Characterization of factor XII Tenri, a rare CRM-negative factor XII deficiencyNoriko Fujihara
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Ann Clin Lab Sci 34:218-25. 2004.... - Functional analysis of recombinant Bbeta15C and Bbeta15A fibrinogens demonstrates that Bbeta15G residue plays important roles in FPB release and in lateral aggregation of protofibrilsM Hirota-Kawadobora
Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
J Thromb Haemost 3:983-90. 2005..Finally, our data indicate that the Bbeta15Gly residue plays important roles in FPB release and lateral aggregation of protofibrils... - [Assembly and secretion of mutant fibrinogens with variant gamma-chain C terminal region (gamma313-gamma345)]Fumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
Rinsho Byori 58:772-8. 2010..To study the assembly and secretion of the variant fibrinogens containing aberrant D regions, we established CHO cells producing these four fibrinogens... - [Functional analysis for dysfibrinogenemias, Toyama and Adachi, which have a mutation of Aalpha16Arg-->His (CGT-->CAT) with aberrant fibrinopeptide A release]Keisuke Soya
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
Rinsho Byori 60:499-505. 2012..Moreover, we also speculated that batroxobin-induced clottability was lower than thrombin-induced clottability, because batroxobin cannot induce the so-called "B-knob-b-hole" interaction, which enhances fibrin formation... - Heterozygous Bβ-chain C-terminal 12 amino acid elongation variant, BβX462W (Kyoto VI), showed dysfibrinogenemiaNobuo Okumura
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Japan
Blood Coagul Fibrinolysis 23:87-90. 2012.... - An immunoglobulin A1 that inhibits lactate dehydrogenase activity, with reversal of inhibition by addition of NADHKiyotaka Fujita
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Nagano, Japan
Ann Clin Lab Sci 36:461-8. 2006..Moreover, the possibility exists that part of the patient's IgA1 molecule fits into a pocket of LD in instead of NADH. This is the first report of NADH reversing such LD inhibition... - [Functional analysis of heterozygous plasma dysfibrinogens derived from two families of gammaArg275Cys and three families of gammaArg275His, and haplotype analysis for these families]Yuka Kamijyo
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Japan
Rinsho Byori 57:651-8. 2009..Furthermore haplotype analysis for three families with gammaArg275His suggested that founder of Iida family might be different from that of Otsu II or Shizuoka family... - Analysis of fibrinogen gamma-chain truncations shows the C-terminus, particularly gammaIle387, is essential for assembly and secretion of this multichain proteinNobuo Okumura
Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, The Second Department of Internal Medicine, Shinshu University School of Medicine, Shinshu University Hospital, Matsumoto, Japan
Blood 99:3654-60. 2002..We propose that the loss of residue gamma387 destabilized the structure of gamma chain, preventing assembly of alphagamma and betagamma dimers, essential intermediates in the assembly of normal fibrinogen... - [Analysis of antibody reactivity for FDP D-dimer fragments by Western blotting]Saki Wakabayashi
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Rinsho Byori 56:449-54. 2008..Unfortunately, we did not directly demonstrate these phenomena by WB analysis with two anti-D-dimer antibodies used Nanopia or LPIA reagent. In the near future, we expect that standardization of FDP D-dimer assay will be accomplished... - The Aalpha-chain 6Ile/Val polymorphism is not associated with plasma fibrinogen levels in JapaneseFumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621, Japan
Thromb Res 112:257-9. 2003 - Sialic acid moiety of apolipoprotein E3 at Thr(194) affects its interaction with beta-amyloid(1-42) peptidesMitsutoshi Sugano
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Clin Chim Acta 388:123-9. 2008..ApoE in cerebrospinal fluid is extensively sialylated, and sialylation of certain proteins are known to modulate biological function. We investigated the effects of a sialic acid moiety of apoE on the apoE-Abeta interaction... - [Role of fibrinogen Bbeta-chain D-region 454-458 residues for assembly and secretion of intact fibrinogen]Fumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
Rinsho Byori 59:741-8. 2011.... - Novel beta-thalassemia trait (IVS I-1 G-->C) in a Japanese familyNoriko Fujihara
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Am J Hematol 72:64-6. 2003.... - Recombinant fibrinogen, gamma275Arg-->Cys, exhibits formation of disulfide bond with cysteine and severely impaired D:D interactionsS Ishikawa
Department of Aging Biology, Shinshu University School of Medicine, Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
J Thromb Haemost 2:468-75. 2004..However, the variant D:D interface can interact with that of the normal fibrinogen existing in a heterozygous patient with dysfibrinogenemia... - Quantitative monitoring of single nucleotide mutations by allele-specific quantitative PCR can be used for the assessment of minimal residual disease in patients with hematological malignancies throughout their clinical courseChiaki Taira
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto 390 8621, Japan
Clin Chim Acta 412:53-8. 2011..Single nucleotide mutations associated with leukemogenesis can be considered as applicable MRD markers... - In vitro fibrin clot formation and fibrinolysis using heterozygous plasma fibrinogen from gammaAsn319, Asp320 deletion dysfibrinogen, Otsu IFumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Japan
Thromb Res 118:651-61. 2006..To analyze the relationship between thrombosis and the heterozygous plasma variant fibrinogen, we used purified plasma fibrinogen from the Otsu patient and compared it with a normal control... - Fibrinogen Matsumoto V: a variant with Aalpha19 Arg-->Gly (AGG-->GGG). Comparison between fibrin polymerization stimulated by thrombin or reptilase and fibrin monomer polymerizationH Tanaka
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
Thromb Haemost 85:108-13. 2001..These results indicate 'B' and/or 'b' site of M-V fibrinogen play a more important role in thrombin-catalyzed fibrin polymerization than that of normal control fibrinogen... - Residue gamma153Cys is essential for the formation of the complexes Aalphagamma and Bbetagamma, assembly intermediates for the AalphaBbetagamma complex and intact fibrinogenFumiko Terasawa
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
Clin Chim Acta 353:157-64. 2005..Fibrinogen Matsumoto IV was found in a hypofibrinogenemia caused by a heterozygous missense mutation, i.e., the substitution of the fibrinogen gamma-chain residue Cys153 by Arg... - Immunohistochemical localization of sodium-potassium ATPase in human normal stomach and gastric adenocarcinomasChujiro Kobayashi
First Department of Surgery, School of Medicine, Shinshu University, Matsumoto, 390-8621 Nagano, Japan
Histochem Cell Biol 119:317-22. 2003..Acetic acid-ethanol and paraffin embedding is a useful method for the investigation of the immunohistochemical localization of Na, K-ATPase in normal and diseased tissues... - Co-localization of TFF2 with gland mucous cell mucin in gastric mucous cells and in extracellular mucous gel adherent to normal and damaged gastric mucosaH Ota
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, 3 1 1 Asahi, Matsumoto, Nagano 390 8621, Japan
Histochem Cell Biol 126:617-25. 2006..Co-localization of the TFF2 with gland mucous cell mucin suggests a physical interaction between the TFF2 and gland mucous cell mucin. The TFF2 trapped in the adherent mucins may be responsible for mucosal defense, healing, and repair... - Fibrinogens Kosai and Ogasa: Bbeta15Gly-->Cys (GGT-->TGT) substitution associated with impairment of fibrinopeptide B release and lateral aggregationM Hirota-Kawadobora
Central Clinical Laboratory, Shinshu University Hospital, Matsumoto, Nagano, Japan
J Thromb Haemost 1:275-83. 2003..The impaired functions may be due to the substitution of Cys for Bbetao15Gly plus the existence of some additional disulfide-bonded forms... - Fibrinopeptide A release is necessary for effective B:b interactions in polymerisation of variant fibrinogens with impaired A:a interactionsKeisuke Soya
Department of Clinical Laboratory Investigation, Graduate School of Medicine, Shinshu University, Matsumoto, Japan
Thromb Haemost 109:221-8. 2013..These results demonstrate that FpA release per se is necessary for effective B:b interactions during polymerisation of variant fibrinogens with impaired A:a interactions... - [Comparison of fibrinogen synthesis and secretion between novel variant fibrinogen, nagakute (gamma305Thr --> Ala), and other variants located in gamma305-308 residues]Tamaki Kobayashi
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
Rinsho Byori 60:831-8. 2012..Furthermore, gamma306S-gamma307H residues of the gammaC nodule play crucial roles for protein synthesis and fibrin polymerization... - Predominant apolipoprotein J exists as lipid-poor mixtures in cerebrospinal fluidTakefumi Suzuki
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
Ann Clin Lab Sci 32:369-76. 2002..We concluded that there are at least 9 forms or combinations (including free apolipoproteins) of apoJ, apoE, and apoAl in the CSF... - [College education of medical technologists serving in highly specialized hospitals]Nobuo Okumura
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto
Rinsho Byori 56:612-6. 2008..Finally, the Division of Medical Technology of the School of Health Sciences, Department of Laboratory Medicine of Shinshu University School of Medicine and Shinshu University Hospital cooperate in the education of MT and CLS... - Serotonin receptor antagonist inhibits monocrotaline-induced pulmonary hypertension and prolongs survival in ratsEiji Hironaka
Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan
Cardiovasc Res 60:692-9. 2003..These effects were accompanied by anti-inflammatory and anti-proliferative effects in the lung tissue and marked improvement of pulmonary vascular endothelial dysfunction and activation... - Sequence gamma 377-395(P2), but not gamma 190-202(P1), is the binding site for the alpha MI-domain of integrin alpha M beta 2 in the gamma C-domain of fibrinogenTatiana P Ugarova
Joseph J Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Lerner Research Institute, Cleveland, Ohio 44195, USA
Biochemistry 42:9365-73. 2003..Thus, these data demonstrate the essential role of the P2, but not P1, sequence for binding of gamma C by the alpha(M)I-domain and suggest that the adhesive function of P2 depends on the minimal recognition motif NRLTIG... - Polymerization-defective fibrinogen variant gammaD364A binds knob "A" peptide mimicSheryl R Bowley
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Biochemistry 47:8607-13. 2008.... - Quantitative RT-PCR analysis demonstrates that synthesis of the recombinant fibrinogen is dependent on the transcription and synthesis of gamma-chainMasako Hirota-Kawadobora
Central Clinical Laboratory, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan
Clin Chim Acta 319:67-73. 2002..This quantitative method to measure mRNA may prove very useful for further in vivo analysis of fibrinogen gene transcription... - [Analysis of hypofibrinogenemias found on routine coagulation screening tests and identification of heterozygous dysfibrinogenemia or fibrinogen deficiency]Masako Hirota Kawadobora
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto
Rinsho Byori 55:989-95. 2007..These data and our previous reports suggest that heterozygous patients with dysfibrinogenemia or fibrinogen deficiency do not demonstrate markedly prolonged PT and APTT values, differing from patients with afibrinogenemia... - Identification and properties of glycated monoclonal IgA that affect the fructosamine assayKiyotaka Fujita
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Clin Chem 49:805-8. 2003 - Fibrinogen Otsu I:A gammaAsn319,Asp320 deletion dysfibrinogen identified in an asymptomatic pregnant womanFumiko Terasawa
Thromb Haemost 90:757-8. 2003