Nobuo Okumura

Summary

Affiliation: Shinshu University
Country: Japan

Publications

  1. ncbi Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit--LD-B Matsumoto I and II (LD-B W323R)
    N Okumura
    Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
    Clin Chim Acta 287:163-71. 1999
  2. ncbi A novel variant fibrinogen, deletion of Bbeta111Ser in coiled-coil region, affecting fibrin lateral aggregation
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Japan
    Clin Chim Acta 365:160-7. 2006
  3. ncbi B:b interactions are essential for polymerization of variant fibrinogens with impaired holes 'a'
    N Okumura
    Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Japan
    J Thromb Haemost 5:2352-9. 2007
  4. doi Citrullinated fibrinogen shows defects in FPA and FPB release and fibrin polymerization catalyzed by thrombin
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, Laboratory of Clinical Chemistry, School of Health Sciences, Shinshu University, Japan
    Clin Chim Acta 401:119-23. 2009
  5. ncbi Substitution of the gamma-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking
    Nobuo Okumura
    Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Blood 103:4157-63. 2004
  6. ncbi Evidence that heterodimers exist in the fibrinogen Matsumoto II (gamma308N-->K) proband and participate in fibrin fiber formation
    Nobuo Okumura
    Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Thromb Res 107:157-62. 2002
  7. ncbi Hypofibrinogenemia associated with a heterozygous C-->T nucleotide substitution at position -1138 BP of the 5'-flanking region of the fibrinogen A alpha-chain gene
    N Okumura
    Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Ann N Y Acad Sci 936:526-30. 2001
  8. ncbi Difference in electrophoretic mobility and plasmic digestion profile between four recombinant fibrinogens, gamma 308K, gamma 308I, gamma 308A, and wild type (gamma 308N)
    N Okumura
    Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
    Electrophoresis 21:2309-15. 2000
  9. ncbi In vitro expression demonstrates impaired secretion of the gammaAsn319, Asp320 deletion variant fibrinogen
    Satomi Kani
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, 390 8621, Japan
    Thromb Haemost 94:53-9. 2005
  10. doi A C-terminal amino acid substitution in the gamma-chain caused by a novel heterozygous frameshift mutation (Fibrinogen Matsumoto VII) results in hypofibrinogenaemia
    Noriko Fujihara
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Thromb Haemost 104:213-23. 2010

Collaborators

Detail Information

Publications51

  1. ncbi Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit--LD-B Matsumoto I and II (LD-B W323R)
    N Okumura
    Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
    Clin Chim Acta 287:163-71. 1999
  2. ncbi A novel variant fibrinogen, deletion of Bbeta111Ser in coiled-coil region, affecting fibrin lateral aggregation
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Japan
    Clin Chim Acta 365:160-7. 2006
    ..Functional fibrinogen concentration of a male infant showed <0.50 g/l and we speculated this patient as a dysfibrinogenemia or hypofibrinogenemia...
  3. ncbi B:b interactions are essential for polymerization of variant fibrinogens with impaired holes 'a'
    N Okumura
    Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Japan
    J Thromb Haemost 5:2352-9. 2007
    ..The role of A:a interactions is well established, but the roles of knob:hole interactions A:b, B:b or B:a remain ambiguous...
  4. doi Citrullinated fibrinogen shows defects in FPA and FPB release and fibrin polymerization catalyzed by thrombin
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, Laboratory of Clinical Chemistry, School of Health Sciences, Shinshu University, Japan
    Clin Chim Acta 401:119-23. 2009
    ....
  5. ncbi Substitution of the gamma-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking
    Nobuo Okumura
    Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Blood 103:4157-63. 2004
    ..Together, our results show substitution of Asn308 with a hydrophobic residue altered neither polymer formation nor polymer structure at physiologic calcium concentrations, whereas substitution with lysine altered both...
  6. ncbi Evidence that heterodimers exist in the fibrinogen Matsumoto II (gamma308N-->K) proband and participate in fibrin fiber formation
    Nobuo Okumura
    Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Thromb Res 107:157-62. 2002
    ....
  7. ncbi Hypofibrinogenemia associated with a heterozygous C-->T nucleotide substitution at position -1138 BP of the 5'-flanking region of the fibrinogen A alpha-chain gene
    N Okumura
    Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Ann N Y Acad Sci 936:526-30. 2001
    ..These results suggest that the region adjacent to the -1138C bp of the 5'-flanking region of the fibrinogen A alpha gene is one of the most crucial sites for the transcription of the fibrinogen A alpha gene...
  8. ncbi Difference in electrophoretic mobility and plasmic digestion profile between four recombinant fibrinogens, gamma 308K, gamma 308I, gamma 308A, and wild type (gamma 308N)
    N Okumura
    Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto, Japan
    Electrophoresis 21:2309-15. 2000
    ..In conclusion, a substitution at residue gamma308Asn may cause a conformational change in the gammachain of fragment D affecting polymerization and plasmin cleavage...
  9. ncbi In vitro expression demonstrates impaired secretion of the gammaAsn319, Asp320 deletion variant fibrinogen
    Satomi Kani
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, 390 8621, Japan
    Thromb Haemost 94:53-9. 2005
    ..These results suggest that the reduced level of gammaDelta319,320 fibrinogen in the plasma of the Otsu patient arises from modestly impaired secretion of this variant fibrinogen...
  10. doi A C-terminal amino acid substitution in the gamma-chain caused by a novel heterozygous frameshift mutation (Fibrinogen Matsumoto VII) results in hypofibrinogenaemia
    Noriko Fujihara
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Thromb Haemost 104:213-23. 2010
    ....
  11. doi In vitro transcription of compound heterozygous hypofibrinogenemia Matsumoto IX; first identification of FGB IVS6 deletion of 4 nucleotides and FGG IVS3-2A>G causing abnormal RNA splicing
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, 390 8621, Japan
    Clin Chim Acta 411:1325-9. 2010
    ..To examine the transcription of mRNA from the M IX gene, we cloned the wild-type and mutant genes into expression vectors...
  12. doi Recombinant variant fibrinogens substituted at residues gamma326Cys and gamma339Cys demonstrated markedly impaired secretion of assembled fibrinogen
    Ayumi Haneishi
    Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan
    Thromb Res 124:368-72. 2009
    ....
  13. doi Fibrinopeptide A release is necessary for effective B:b interactions in polymerisation of variant fibrinogens with impaired A:a interactions
    Keisuke Soya
    Department of Clinical Laboratory Investigation, Graduate School of Medicine, Shinshu University, Matsumoto, Japan
    Thromb Haemost 109:221-8. 2013
    ..These results demonstrate that FpA release per se is necessary for effective B:b interactions during polymerisation of variant fibrinogens with impaired A:a interactions...
  14. ncbi Analysis of fibrinogen variants at gamma387Ile shows that the side chain of gamma387 and the tertiary structure of the gammaC-terminal tail are important not only for assembly and secretion of fibrinogen but also for lateral aggregation of protofibrils an
    Satomi Kani
    Laboratory of Clinical Chemistry, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Blood 108:1887-94. 2006
    ..Interestingly, our results clearly indicate that the conformations critical for these 2 functions are different from each other...
  15. doi Molecular analysis of afibrinogenemic mutations caused by a homozygous FGA1238 bp deletion, and a compound heterozygous FGA1238 bp deletion and novel FGA c.54+3A>C substitution
    Yuka Takezawa
    Department of Health and Medical Sciences, Graduate School of Medicine, Shinshu University, Matsumoto, Japan
    Int J Hematol 96:39-46. 2012
    ..Molecular analyses of both genetic variants suggest that the lack of a mature Aα-chain, impaired assembly, and/or secretion of the fibrinogen molecule may lead to afibrinogenemia...
  16. ncbi In vitro expression of beta-thalassaemia gene (IVS1-1G>C) reveals complete inactivation of the normal 5' splice site and alternative aberrant RNA splicing
    Noriko Fujihara
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Ann Clin Biochem 44:573-8. 2007
    ..Further studies will be required to clarify the molecular mechanism that results in severe reduction in the mutant mRNA level in vivo...
  17. ncbi Nonsense-mediated mRNA decay was demonstrated in two hypofibrinogenemias caused by heterozygous nonsense mutations of FGG, Shizuoka III and Kanazawa II
    Keisuke Soya
    Department of Health and Medical Sciences, Graduate School of Medicine, Shinshu University, Japan
    Thromb Res 132:465-70. 2013
    ..Finally, these results demonstrated that mRNAs containing γ23X or γ376X are degraded by the NMD system and translation of the truncated γ-chain polypeptide decrease in patients' hepatocytes, resulting in hypofibrinogenemias. ..
  18. doi Application of allele-specific quantitative PCR using genomic DNA to monitor minimal residual disease based on mutant gene levels following allogeneic hematopoietic stem cell transplantation in patients with hematological malignancies: comparison of mutan
    Chiaki Taira
    Department of Laboratory Medicine, Shinshu University Hospital, Asahi, Matsumoto, Japan
    Clin Chim Acta 413:516-9. 2012
    ....
  19. ncbi Characterization of factor XII Tenri, a rare CRM-negative factor XII deficiency
    Noriko Fujihara
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Ann Clin Lab Sci 34:218-25. 2004
    ....
  20. ncbi Functional analysis of recombinant Bbeta15C and Bbeta15A fibrinogens demonstrates that Bbeta15G residue plays important roles in FPB release and in lateral aggregation of protofibrils
    M Hirota-Kawadobora
    Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
    J Thromb Haemost 3:983-90. 2005
    ..To eliminate the influence of plasma heterozygous molecules, we synthesized and analyzed recombinant-variant fibrinogens...
  21. ncbi siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the Aα-chain gene does not affect the plasma fibrinogen level
    Yuka Takezawa
    Department of Health and Medical Sciences, Graduate School of Medicine, Shinshu University, Japan
    Thromb Res 131:342-8. 2013
    ..Of interest, the patients' parents, who are heterozygous, had normal levels of plasma fibrinogen; thus, we hypothesized that liver FGA mRNA levels were higher than those of FGB and/or FGG mRNA...
  22. ncbi Quantitative RT-PCR analysis demonstrates that synthesis of the recombinant fibrinogen is dependent on the transcription and synthesis of gamma-chain
    Masako Hirota-Kawadobora
    Central Clinical Laboratory, Shinshu University Hospital, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Clin Chim Acta 319:67-73. 2002
    ..The purpose of this study was to examine the relationship between the production of secreted fibrinogen and the synthesis of gamma-chain mRNA...
  23. ncbi Novel beta-thalassemia trait (IVS I-1 G-->C) in a Japanese family
    Noriko Fujihara
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Am J Hematol 72:64-6. 2003
    ....
  24. ncbi Analysis of fibrinogen gamma-chain truncations shows the C-terminus, particularly gammaIle387, is essential for assembly and secretion of this multichain protein
    Nobuo Okumura
    Laboratory of Clinical Chemistry, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, The Second Department of Internal Medicine, Shinshu University School of Medicine, Shinshu University Hospital, Matsumoto, Japan
    Blood 99:3654-60. 2002
    ..We propose that the loss of residue gamma387 destabilized the structure of gamma chain, preventing assembly of alphagamma and betagamma dimers, essential intermediates in the assembly of normal fibrinogen...
  25. ncbi [Assembly and secretion of mutant fibrinogens with variant gamma-chain C terminal region (gamma313-gamma345)]
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
    Rinsho Byori 58:772-8. 2010
    ..To study the assembly and secretion of the variant fibrinogens containing aberrant D regions, we established CHO cells producing these four fibrinogens...
  26. ncbi [Functional analysis for dysfibrinogenemias, Toyama and Adachi, which have a mutation of Aalpha16Arg-->His (CGT-->CAT) with aberrant fibrinopeptide A release]
    Keisuke Soya
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
    Rinsho Byori 60:499-505. 2012
    ..Moreover, we also speculated that batroxobin-induced clottability was lower than thrombin-induced clottability, because batroxobin cannot induce the so-called "B-knob-b-hole" interaction, which enhances fibrin formation...
  27. doi Heterozygous Bβ-chain C-terminal 12 amino acid elongation variant, BβX462W (Kyoto VI), showed dysfibrinogenemia
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Japan
    Blood Coagul Fibrinolysis 23:87-90. 2012
    ....
  28. ncbi [Functional analysis of heterozygous plasma dysfibrinogens derived from two families of gammaArg275Cys and three families of gammaArg275His, and haplotype analysis for these families]
    Yuka Kamijyo
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Japan
    Rinsho Byori 57:651-8. 2009
    ..Furthermore haplotype analysis for three families with gammaArg275His suggested that founder of Iida family might be different from that of Otsu II or Shizuoka family...
  29. ncbi [Analysis of antibody reactivity for FDP D-dimer fragments by Western blotting]
    Saki Wakabayashi
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Rinsho Byori 56:449-54. 2008
    ..Unfortunately, we did not directly demonstrate these phenomena by WB analysis with two anti-D-dimer antibodies used Nanopia or LPIA reagent. In the near future, we expect that standardization of FDP D-dimer assay will be accomplished...
  30. ncbi Sialic acid moiety of apolipoprotein E3 at Thr(194) affects its interaction with beta-amyloid(1-42) peptides
    Mitsutoshi Sugano
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Clin Chim Acta 388:123-9. 2008
    ..ApoE in cerebrospinal fluid is extensively sialylated, and sialylation of certain proteins are known to modulate biological function. We investigated the effects of a sialic acid moiety of apoE on the apoE-Abeta interaction...
  31. ncbi The Aalpha-chain 6Ile/Val polymorphism is not associated with plasma fibrinogen levels in Japanese
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Thromb Res 112:257-9. 2003
  32. ncbi An immunoglobulin A1 that inhibits lactate dehydrogenase activity, with reversal of inhibition by addition of NADH
    Kiyotaka Fujita
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Nagano, Japan
    Ann Clin Lab Sci 36:461-8. 2006
    ..Moreover, the possibility exists that part of the patient's IgA1 molecule fits into a pocket of LD in instead of NADH. This is the first report of NADH reversing such LD inhibition...
  33. ncbi [Role of fibrinogen Bbeta-chain D-region 454-458 residues for assembly and secretion of intact fibrinogen]
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
    Rinsho Byori 59:741-8. 2011
    ....
  34. ncbi Recombinant fibrinogen, gamma275Arg-->Cys, exhibits formation of disulfide bond with cysteine and severely impaired D:D interactions
    S Ishikawa
    Department of Aging Biology, Shinshu University School of Medicine, Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    J Thromb Haemost 2:468-75. 2004
    ..Since this substitution occurred in a heterozygous individual, interpretation of the functional analysis was complicated. We tried to resolve this complication by synthesizing a recombinant variant fibrinogen...
  35. ncbi [Comparison of fibrinogen synthesis and secretion between novel variant fibrinogen, nagakute (gamma305Thr --> Ala), and other variants located in gamma305-308 residues]
    Tamaki Kobayashi
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto 390 8621, Japan
    Rinsho Byori 60:831-8. 2012
    ..Furthermore, gamma306S-gamma307H residues of the gammaC nodule play crucial roles for protein synthesis and fibrin polymerization...
  36. doi Quantitative monitoring of single nucleotide mutations by allele-specific quantitative PCR can be used for the assessment of minimal residual disease in patients with hematological malignancies throughout their clinical course
    Chiaki Taira
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto 390 8621, Japan
    Clin Chim Acta 412:53-8. 2011
    ..Single nucleotide mutations associated with leukemogenesis can be considered as applicable MRD markers...
  37. ncbi In vitro fibrin clot formation and fibrinolysis using heterozygous plasma fibrinogen from gammaAsn319, Asp320 deletion dysfibrinogen, Otsu I
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto, Japan
    Thromb Res 118:651-61. 2006
    ..To analyze the relationship between thrombosis and the heterozygous plasma variant fibrinogen, we used purified plasma fibrinogen from the Otsu patient and compared it with a normal control...
  38. ncbi Fibrinogen Matsumoto V: a variant with Aalpha19 Arg-->Gly (AGG-->GGG). Comparison between fibrin polymerization stimulated by thrombin or reptilase and fibrin monomer polymerization
    H Tanaka
    Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
    Thromb Haemost 85:108-13. 2001
    ..These results indicate 'B' and/or 'b' site of M-V fibrinogen play a more important role in thrombin-catalyzed fibrin polymerization than that of normal control fibrinogen...
  39. ncbi Residue gamma153Cys is essential for the formation of the complexes Aalphagamma and Bbetagamma, assembly intermediates for the AalphaBbetagamma complex and intact fibrinogen
    Fumiko Terasawa
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3 1 1 Asahi, Matsumoto 390 8621, Japan
    Clin Chim Acta 353:157-64. 2005
    ..Fibrinogen Matsumoto IV was found in a hypofibrinogenemia caused by a heterozygous missense mutation, i.e., the substitution of the fibrinogen gamma-chain residue Cys153 by Arg...
  40. ncbi Immunohistochemical localization of sodium-potassium ATPase in human normal stomach and gastric adenocarcinomas
    Chujiro Kobayashi
    First Department of Surgery, School of Medicine, Shinshu University, Matsumoto, 390 8621 Nagano, Japan
    Histochem Cell Biol 119:317-22. 2003
    ..Acetic acid-ethanol and paraffin embedding is a useful method for the investigation of the immunohistochemical localization of Na, K-ATPase in normal and diseased tissues...
  41. ncbi Co-localization of TFF2 with gland mucous cell mucin in gastric mucous cells and in extracellular mucous gel adherent to normal and damaged gastric mucosa
    H Ota
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, 3 1 1 Asahi, Matsumoto, Nagano 390 8621, Japan
    Histochem Cell Biol 126:617-25. 2006
    ..Co-localization of the TFF2 with gland mucous cell mucin suggests a physical interaction between the TFF2 and gland mucous cell mucin. The TFF2 trapped in the adherent mucins may be responsible for mucosal defense, healing, and repair...
  42. doi γ375W fibrinogen-synthesizing CHO cells indicate the accumulation of variant fibrinogen within endoplasmic reticulum
    Tamaki Kobayashi
    Department of Clinical Laboratory Investigation, Shinshu University, Matsumoto, Japan
    Thromb Res 133:101-7. 2014
    ..Of interest, substitution of γR375W induced hypofibrinogenemia and HERSD, whereas γR375G induced dysfibrinogenemia...
  43. ncbi Predominant apolipoprotein J exists as lipid-poor mixtures in cerebrospinal fluid
    Takefumi Suzuki
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan
    Ann Clin Lab Sci 32:369-76. 2002
    ..We concluded that there are at least 9 forms or combinations (including free apolipoproteins) of apoJ, apoE, and apoAl in the CSF...
  44. ncbi Fibrinogens Kosai and Ogasa: Bbeta15Gly-->Cys (GGT-->TGT) substitution associated with impairment of fibrinopeptide B release and lateral aggregation
    M Hirota-Kawadobora
    Central Clinical Laboratory, Shinshu University Hospital, Matsumoto, Nagano, Japan
    J Thromb Haemost 1:275-83. 2003
    ..The impaired functions may be due to the substitution of Cys for Bbetao15Gly plus the existence of some additional disulfide-bonded forms...
  45. ncbi [College education of medical technologists serving in highly specialized hospitals]
    Nobuo Okumura
    Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto
    Rinsho Byori 56:612-6. 2008
    ..Finally, the Division of Medical Technology of the School of Health Sciences, Department of Laboratory Medicine of Shinshu University School of Medicine and Shinshu University Hospital cooperate in the education of MT and CLS...
  46. ncbi Serotonin receptor antagonist inhibits monocrotaline-induced pulmonary hypertension and prolongs survival in rats
    Eiji Hironaka
    Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan
    Cardiovasc Res 60:692-9. 2003
    ..The purpose of the present study was to examine the role of a 5-HT2A receptor antagonist, sarpogrelate hydrochroride, in preventing or reversing monocrotaline (MCT)-induced PAH in rats...
  47. doi Polymerization-defective fibrinogen variant gammaD364A binds knob "A" peptide mimic
    Sheryl R Bowley
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:8607-13. 2008
    ....
  48. ncbi [Analysis of hypofibrinogenemias found on routine coagulation screening tests and identification of heterozygous dysfibrinogenemia or fibrinogen deficiency]
    Masako Hirota-Kawadobora
    Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto
    Rinsho Byori 55:989-95. 2007
    ..These data and our previous reports suggest that heterozygous patients with dysfibrinogenemia or fibrinogen deficiency do not demonstrate markedly prolonged PT and APTT values, differing from patients with afibrinogenemia...
  49. ncbi Sequence gamma 377-395(P2), but not gamma 190-202(P1), is the binding site for the alpha MI-domain of integrin alpha M beta 2 in the gamma C-domain of fibrinogen
    Tatiana P Ugarova
    Joseph J Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, Lerner Research Institute, Cleveland, Ohio 44195, USA
    Biochemistry 42:9365-73. 2003
    ..Thus, these data demonstrate the essential role of the P2, but not P1, sequence for binding of gamma C by the alpha(M)I-domain and suggest that the adhesive function of P2 depends on the minimal recognition motif NRLTIG...
  50. ncbi Identification and properties of glycated monoclonal IgA that affect the fructosamine assay
    Kiyotaka Fujita
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Clin Chem 49:805-8. 2003
  51. ncbi Fibrinogen Otsu I:A gammaAsn319,Asp320 deletion dysfibrinogen identified in an asymptomatic pregnant woman
    Fumiko Terasawa
    Thromb Haemost 90:757-8. 2003