Research Topics
| Naoki ShinodaSummaryCountry: Japan Publications
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Detail Information
Publications
Developing PCR primers using a new computer program for detection of multiple animal-derived materials in feedNaoki Shinoda
Food and Agricultural Materials Inspection Center, 2 1 Shintoshin, Chuo Ku, Saitama shi, Saitama 330 9731, Japan
J Food Prot 71:2257-62. 2008..05 to 0.01% bovine meat and bone meal and 0.1 pg of bovine DNA. We also successfully applied the primer set to 17 commercial feed samples that were known to be free from ruminant-derived materials. No false-positive results were found...
Development of primers for detection of heat-treated cetacean materials in porcine meat and bone mealNaoki Shinoda
Food and Agricultural Materials Inspection Center, 2 1 Shintoshin, Chuo Ku, Saitama shi, Saitama 330 9731, Japan
J Food Prot 72:1496-9. 2009..The primer sets were capable of detecting whale meat meal that had been autoclaved at 133 degrees C for up to 20 min. The detection limit of whale material in porcine meat and bone meal was 0.1%...
High GC contents of primer 5'-end increases reaction efficiency in polymerase chain reactionNaoki Shinoda
Food and Agricultural Materials Inspection Center, Saitama, Japan
Nucleosides Nucleotides Nucleic Acids 28:324-30. 2009..As a result, there were significant correlations between the amplification signals and the GC contents in the first 1 approximately 3 bps of primer 5'-end...
PCR-RFLP identification of prohibited animal derived DNA in animal feedNaoki Shinoda
Food and Agricultural Materials Inspection Center, Chuo Ku, Saitama shi, Saitama, Japan
Shokuhin Eiseigaku Zasshi 52:24-7. 2011..This PCR-RFLP method, which ensures identification of prohibited animal species in feed, is useful for enhancing the reliability of feed inspection for BSE prevention. This method will be added to the Official Methods of Feed Analysis...
Development of PCR primers for the detection of porcine DNA in feed using mtATP6 as the target sequenceTomotaro Yoshida
Food and Agricultural Materials Inspection Center, Chuo Ku, Saitama 3309731, Japan
Shokuhin Eiseigaku Zasshi 50:89-92. 2009..PPA6 was superior to PPA8 in terms of detection of DNA damaged/fragmented during rendering procedures. The PCR method using these primer sets is registered as the official analytical method for feed in Japan...
Risk of introduction of BSE into Japan by the historical importation of live cattle from the United KingdomKatsuaki Sugiura
Food and Agricultural Materials Inspection Center, Saitama, Saitama, Japan
J Vet Med Sci 71:133-8. 2009..The amount of infectivity that entered the feed chain in 1989, 1991 and 1995 was smaller but still substantial, suggesting that the BSE agent might have entered the Japanese feed chain in any of these years...
Impact of potential changes to the current bovine spongiform encephalopathy surveillance programs for slaughter cattle and fallen stock in JapanKatsuaki Sugiura
Food and Agricultural Materials Inspection Center, 2 1 Shintoshin, Chuo Ku, Saitama, Saitama 330 9731, Japan
J Food Prot 72:1463-7. 2009..The probability of infected Wagyu-Holstein crosses and Holstein steers being detected at slaughter or as fallen stock would be very low under all surveillance strategies...
