Jisaka M, Kim R, Boeglin W, Brash A. Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. J Biol Chem. 2000;275:1287-93 pubmed
..Thus, this positional determinant of the 8-LOX and 15-LOX-2 may have significance for other lipoxygenases. ..
Jisaka M, Boeglin W, Kim R, Brash A. Site-directed mutagenesis studies on a putative fifth iron ligand of mouse 8S-lipoxygenase: retention of catalytic activity on mutation of serine-558 to asparagine, histidine, or alanine. Arch Biochem Biophys. 2001;386:136-42 pubmed
..We conclude that mouse 8-LOX is catalytically competent with only four amino acid iron ligands, and that Ser-558 of the wild-type enzyme does not play an essential role in catalysis. ..
Jisaka M, Iwanaga C, Takahashi N, Goto T, Kawada T, Yamamoto T, et al. Double dioxygenation by mouse 8S-lipoxygenase: specific formation of a potent peroxisome proliferator-activated receptor alpha agonist. Biochem Biophys Res Commun. 2005;338:136-43 pubmed
..The present results suggest that 8S,15S-diH(P)ETE as well as 8S-H(P)ETE would contribute to the physiological function of 8-LOX and also that 8-LOX can function as a potential 15-LOX. ..