Shinji Yamashita

Summary

Affiliation: Setsunan University
Country: Japan

Publications

  1. doi request reprint PET imaging of the gastrointestinal absorption of orally administered drugs in conscious and anesthetized rats
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    J Nucl Med 52:249-56. 2011
  2. doi request reprint Measurement of drug concentration in the stomach after intragastric administration of drug solution to healthy volunteers: analysis of intragastric fluid dynamics and drug absorption
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotobe cho, Hirakata, Osaka, 573 0101, Japan
    Pharm Res 30:951-8. 2013
  3. ncbi request reprint Optimized conditions for prediction of intestinal drug permeability using Caco-2 cells
    S Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, Nagaotoge cho 45 1, Hirakata, Osaka, Japan
    Eur J Pharm Sci 10:195-204. 2000
  4. ncbi request reprint New and better protocols for a short-term Caco-2 cell culture system
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Pharm Sci 91:669-79. 2002
  5. doi request reprint Analysis of risk factors in human bioequivalence study that incur bioinequivalence of oral drug products
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573 0101, Japan
    Mol Pharm 6:48-59. 2009
  6. doi request reprint Detection of early colorectal cancer imaged with peanut agglutinin-immobilized fluorescent nanospheres having surface poly(N-vinylacetamide) chains
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Eur J Pharm Biopharm 74:451-60. 2010
  7. doi request reprint Essence of affinity and specificity of peanut agglutinin-immobilized fluorescent nanospheres with surface poly(N-vinylacetamide) chains for colorectal cancer
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Eur J Pharm Biopharm 79:537-43. 2011
  8. pmc A potential of peanut agglutinin-immobilized fluorescent nanospheres as a safe candidate of diagnostic drugs for colonoscopy
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Eur J Pharm Sci 42:340-7. 2011
  9. ncbi request reprint Cell-penetrating peptide-linked polymers as carriers for mucosal vaccine delivery
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    Mol Pharm 9:2933-41. 2012
  10. doi request reprint Performance of cell-penetrating peptide-linked polymers physically mixed with poorly membrane-permeable molecules on cell membranes
    Shinji Sakuma
    Department of Pharmaceutics, Setsunan University, University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    Eur J Pharm Biopharm 81:64-73. 2012

Detail Information

Publications39

  1. doi request reprint PET imaging of the gastrointestinal absorption of orally administered drugs in conscious and anesthetized rats
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    J Nucl Med 52:249-56. 2011
    ..This study assessed the process of gastrointestinal drug absorption in vivo using PET...
  2. doi request reprint Measurement of drug concentration in the stomach after intragastric administration of drug solution to healthy volunteers: analysis of intragastric fluid dynamics and drug absorption
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotobe cho, Hirakata, Osaka, 573 0101, Japan
    Pharm Res 30:951-8. 2013
    ..To evaluate the time-profile of intragastric fluid volume in humans after intragastric administration of drug solution...
  3. ncbi request reprint Optimized conditions for prediction of intestinal drug permeability using Caco-2 cells
    S Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, Nagaotoge cho 45 1, Hirakata, Osaka, Japan
    Eur J Pharm Sci 10:195-204. 2000
    ....
  4. ncbi request reprint New and better protocols for a short-term Caco-2 cell culture system
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Pharm Sci 91:669-79. 2002
    ....
  5. doi request reprint Analysis of risk factors in human bioequivalence study that incur bioinequivalence of oral drug products
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573 0101, Japan
    Mol Pharm 6:48-59. 2009
    ..In the case of generic drugs, since the parameter of AUC/dose is available before initiating human BE study, this finding is expected to promote an efficient and cost-saving strategy for the development of oral drug products...
  6. doi request reprint Detection of early colorectal cancer imaged with peanut agglutinin-immobilized fluorescent nanospheres having surface poly(N-vinylacetamide) chains
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Eur J Pharm Biopharm 74:451-60. 2010
    ..This good correlation showed that PNA-immobilized fluorescent nanospheres recognized millimeter-sized tumors on the cecal mucosa with high affinity and specificity...
  7. doi request reprint Essence of affinity and specificity of peanut agglutinin-immobilized fluorescent nanospheres with surface poly(N-vinylacetamide) chains for colorectal cancer
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Eur J Pharm Biopharm 79:537-43. 2011
    ..In vivo data proved that PNA and PNVA were essential for biorecognition for tumor tissues and a reduction of nonspecific interactions with normal tissues, respectively...
  8. pmc A potential of peanut agglutinin-immobilized fluorescent nanospheres as a safe candidate of diagnostic drugs for colonoscopy
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Eur J Pharm Sci 42:340-7. 2011
    ..Therefore, we concluded that the imaging agent is a safe and stable probe which remains in the large intestine without systemic exposure...
  9. ncbi request reprint Cell-penetrating peptide-linked polymers as carriers for mucosal vaccine delivery
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    Mol Pharm 9:2933-41. 2012
    ..Our cell-penetrating peptide-linked polymer is a potential candidate for antigen carriers that induce humoral immunity on the mucosal surface and in systemic circulation when nasally coadministered with antigens...
  10. doi request reprint Performance of cell-penetrating peptide-linked polymers physically mixed with poorly membrane-permeable molecules on cell membranes
    Shinji Sakuma
    Department of Pharmaceutics, Setsunan University, University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    Eur J Pharm Biopharm 81:64-73. 2012
    ....
  11. doi request reprint Correlation between in vitro dissolution profiles from enteric-coated dosage forms and in vivo absorption in rats for high-solubility and high-permeability model drugs
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaokatoge cho, Hirakata, Osaka 573 0101, Japan
    J Pharm Sci 98:4141-52. 2009
    ..Thus, a good IVIVC was generally obtained; however, the exception suggests the importance of developing a dissolution test that fully reflects the in vivo situation...
  12. ncbi request reprint Lectin-immobilized fluorescent nanospheres for targeting to colorectal cancer from a physicochemical perspective
    Shinji Sakuma
    Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Curr Drug Discov Technol 8:367-78. 2011
    ..This review describes the design concept of this nanoprobe from a physicochemical perspective...
  13. doi request reprint Carboxyl group-terminated polyamidoamine dendrimers bearing glucosides inhibit intestinal hexose transporter-mediated D-glucose uptake
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Eur J Pharm Biopharm 75:366-74. 2010
    ..The dendritic conjugate bearing arbutin appears to be a good candidate as an oral anti-diabetic drug...
  14. ncbi request reprint Effect of P-glycoprotein expression levels on the concentration-dependent permeability of drugs to the cell membrane
    Yoshiyuki Shirasaka
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Pharm Sci 97:553-65. 2008
    ..This study clearly demonstrated the possibility to estimate the permeability of P-gp substrate drugs in human intestine from the expression level of P-gp, and thus the possibility to predict oral absorption of those drugs...
  15. doi request reprint Estimation of P-glycoprotein-mediated efflux in the oral absorption of P-gp substrate drugs from simultaneous analysis of drug dissolution and permeation
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, Japan
    Eur J Pharm Sci 44:544-51. 2011
    ..These estimations corresponded well to in vivo human observations. Our in vitro system is useful in assessment of the effect of P-gp-mediated efflux on in vivo oral absorption of P-gp substrates...
  16. doi request reprint Oligoarginine-linked polymers as a new class of penetration enhancers
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Control Release 148:187-96. 2010
    ..Results demonstrated that our oligoarginine-linked polymer has a potential to provide a new class of penetration enhancers...
  17. ncbi request reprint Effect of food intake on the oral absorption of poorly water-soluble drugs: in vitro assessment of drug dissolution and permeation assay system
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573 0101, Japan
    J Pharm Sci 95:2051-61. 2006
    ..In conclusion, the D/P system was proved to be a useful assay system not only for the oral absorption of drugs, but also for the food effect on the absorption...
  18. doi request reprint Scaling of in vitro membrane permeability to predict P-glycoprotein-mediated drug absorption in vivo
    Yoshiyuki Shirasaka
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Drug Metab Dispos 36:916-22. 2008
    ..This study clearly demonstrated the potential to estimate the permeability of P-gp substrate drugs in the human intestine from its P-gp expression level and thus the possibility to predict the oral absorption of those drugs...
  19. doi request reprint Application of dissolution/permeation system for evaluation of formulation effect on oral absorption of poorly water-soluble drugs in drug development
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka, 573 0101, Japan
    Pharm Res 29:1485-94. 2012
    ..The aim of the present study is to evaluate the formulation effect on the oral absorption of poorly water-soluble drugs using a dissolution/permeation system (D/P system)...
  20. doi request reprint Scale-up of in vitro permeation assay data to human intestinal permeability using pore theory
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Int J Pharm 414:69-76. 2011
    ..A linear correlation whose slope and intercept were nearly 1 and 0, respectively, was observed between predicted and reported human intestinal permeabilities. We successfully predicted human intestinal permeability from in vitro data...
  21. ncbi request reprint In vitro system to evaluate oral absorption of poorly water-soluble drugs: simultaneous analysis on dissolution and permeation of drugs
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, Nagaotoge cho 45 1, Hirakata, Osaka 573 0101, Japan
    Pharm Res 20:1674-80. 2003
    ..The aim of the present work was to develop a new in vitro system to evaluate oral absorption of poorly water-soluble drugs by utilizing Caco-2 monolayers...
  22. doi request reprint Involvement of functional groups on the surface of carboxyl group-terminated polyamidoamine dendrimers bearing arbutin in inhibition of Na⁺/glucose cotransporter 1 (SGLT1)-mediated D-glucose uptake
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Mol Pharm 9:922-9. 2012
    ....
  23. ncbi request reprint In vitro dissolution/permeation system to predict the oral absorption of poorly water-soluble drugs: effect of food and dose strength on it
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573 0101, Japan
    Biol Pharm Bull 34:401-7. 2011
    ..Consequently, the D/P system was demonstrated to be a useful in vitro system for prediction of the oral absorption of poorly water-soluble drugs...
  24. doi request reprint Expression levels of human P-glycoprotein in in vitro cell lines: correlation between mRNA and protein levels for P-glycoprotein expressed in cells
    Yoshiyuki Shirasaka
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan
    Biopharm Drug Dispos 30:149-52. 2009
    ..From these findings, it was suggested that P-gp activity in MDCKII/MDR1 cells could not be estimated from its mRNA levels...
  25. ncbi request reprint Site of drug absorption after oral administration: assessment of membrane permeability and luminal concentration of drugs in each segment of gastrointestinal tract
    Yoshie Masaoka
    College of Pharmacy, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Eur J Pharm Sci 29:240-50. 2006
    ..These informations on site-dependent absorption of drugs are considered to be important for effective oral delivery systems...
  26. doi request reprint Stabilization of enzyme-susceptible glucoside bonds of phloridzin through conjugation with poly(gamma-glutamic acid)
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan
    J Control Release 133:125-31. 2009
    ..We also concluded that the toxic phloretin was not released from the conjugates. These results suggest that gamma-PGA-phloridzin conjugates have potential as oral anti-diabetic drugs with high safety...
  27. ncbi request reprint Conjugation of poorly absorptive drugs with mucoadhesive polymers for the improvement of oral absorption of drugs
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Control Release 123:195-202. 2007
    ..5-fold increase in the oral absorption with statistical significance. This novel approach has a potential to improve oral absorption of drugs with poorly absorptive properties caused by low membrane permeability...
  28. ncbi request reprint Effect of administration site in the gastrointestinal tract on bioavailability of poorly absorbed drugs taken after a meal
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Control Release 118:59-64. 2007
    ..Site-specific drug delivery to the middle small intestine could be a useful approach for reducing the negative food effect on drug absorption with maximized bioavailability...
  29. doi request reprint A perspective for biowaivers of human bioequivalence studies on the basis of the combination of the ratio of AUC to the dose and the biopharmaceutics classification system
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1, Nagaokatoge cho, Hirakata, Osaka 573 0101, Japan
    Mol Pharm 8:1113-9. 2011
    ..Our analysis indicates the appropriateness of biowaiver of bioequivalence studies for immediate-release solid oral dosage forms containing not only BCS class 1 drugs but also class 3 drugs...
  30. ncbi request reprint Induction of human P-glycoprotein in Caco-2 cells: development of a highly sensitive assay system for P-glycoprotein-mediated drug transport
    Yoshiyuki Shirasaka
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan
    Drug Metab Pharmacokinet 21:414-23. 2006
    ..In conclusion, this study provided a stable and highly sensitive in vitro assay system that can identify compounds that are subject to P-gp-mediated efflux...
  31. ncbi request reprint Transnasal delivery of 5-fluorouracil to the brain in the rat
    T Sakane
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan
    J Drug Target 7:233-40. 1999
    ..94 microl/min/g tissue) was not. Consequently, significant amount of 5FU is transported from the nasal cavity to the brain through the CSF and thus, the delivery of the hydrophilic drug to the brain is augmented by nasal drug application...
  32. ncbi request reprint Establishment of MDCKII cell monolayer with metabolic activity by CYP3A4 transduced with recombinant adenovirus
    Makoto Kataoka
    Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Japan
    Drug Metab Pharmacokinet 28:125-31. 2013
    ..This study indicates that monolayers of MDCKII cells transduced with AdCYP3A4 have the potential to enable estimation of the first-pass metabolism by CYP3A4 in the intestinal absorption process...
  33. ncbi request reprint Evaluation of human P-glycoprotein (MDR1/ABCB1) ATPase activity assay method by comparing with in vitro transport measurements: Michaelis-Menten kinetic analysis to estimate the affinity of P-glycoprotein to drugs
    Yoshiyuki Shirasaka
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Biol Pharm Bull 29:2465-71. 2006
    ..The combining of the ATPase activity assay and in vitro transport technologies provides us the insight into mechanisms of the membrane transport of drugs by P-gp...
  34. doi request reprint In vitro/in vivo biorecognition of lectin-immobilized fluorescent nanospheres for human colorectal cancer cells
    Shinji Sakuma
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    J Control Release 134:2-10. 2009
    ..Data indicated that imaging agents bound to colorectal cancer cells and the cancer cell-derived tumors with high affinity and specificity...
  35. ncbi request reprint Effect of experimental acute renal and hepatic failure on absorption of tacrolimus in rat small intestine
    Shigeki Tamura
    Fujisawa Pharmaceutical Co, Ltd, Osaka, Japan
    Drug Metab Pharmacokinet 19:190-7. 2004
    ..This type of kinetic study in rats should be valuable to identify the precise mechanisms of drug absorption and the effects of various diseases on it, such as acute renal or hepatic failure...
  36. ncbi request reprint Direct drug transport from the rat nasal cavity to the cerebrospinal fluid: the relation to the dissociation of the drug
    T Sakane
    Faculty of Pharmaceutical Sciences, Setsunan University, Japan
    J Pharm Pharmacol 46:378-9. 1994
    ..These results show that both the nasal absorption and the drug transport conform to the pH partition theory...
  37. doi request reprint Transnasal delivery of methotrexate to brain tumors in rats: a new strategy for brain tumor chemotherapy
    Tomotaka Shingaki
    ADME Research Inc, 1 12 8 Senba higashi, Minoh, Osaka 562 0035, Japan
    Mol Pharm 7:1561-8. 2010
    ..The strategy to utilize the nose-brain direct transport can be applicable to a new therapeutic system not only for brain tumors but also for other central nervous system disorders such as neurodegenerative diseases...
  38. ncbi request reprint Absorption and metabolism of antioxidative polyphenolic compounds in red wine
    Shinji Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotouge cho, Hirakata City Osaka 573 0101, Japan
    Ann N Y Acad Sci 957:325-8. 2002
    ....
  39. ncbi request reprint Analysis of drug permeation across Caco-2 monolayer: implication for predicting in vivo drug absorption
    S Yamashita
    Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan
    Pharm Res 14:486-91. 1997
    ..The aim of the present work is to characterize in vitro drug permeation processes across Caco-2 monolayer and to identify the advantages of this cultured cell system in predicting in vivo drug absorption after oral administration...