Akon Higuchi

Summary

Affiliation: Seikei University
Country: Japan

Publications

  1. ncbi request reprint Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes
    Seog Jin Seo
    School of Agricultural Biotechnology, Seoul National University, Seoul, Korea
    Tissue Eng 12:33-44. 2006
  2. ncbi request reprint Bioinert surface of pluronic-immobilized flask for preservation of hematopoietic stem cells
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 7:1083-9. 2006
  3. ncbi request reprint Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 1 Kichijoji kitamachi 3, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 78:491-9. 2006
  4. ncbi request reprint Temperature-induced cell detachment on immobilized pluronic surface
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 79:380-92. 2006
  5. pmc Visible light regulates neurite outgrowth of nerve cells
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, No 300, Jhongda Rd, Jhongli, Taoyuan, 32001, Taiwan
    Cytotechnology 54:181-8. 2007
  6. ncbi request reprint Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 85:853-61. 2008
  7. ncbi request reprint Cell separation between mesenchymal progenitor cells through porous polymeric membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, Musashino, Tokyo, Japan
    J Biomed Mater Res B Appl Biomater 74:511-9. 2005
  8. doi request reprint Preservation of hematopoietic stem and progenitor cells from umbilical cord blood stored in a surface derivatized with polymer nanosegments
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, 32001 Taiwan
    Biomacromolecules 9:634-9. 2008
  9. ncbi request reprint Albumin and urea production by hepatocytes cultured on extracellular matrix proteins-conjugated poly(vinyl alcohol) membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomater Sci Polym Ed 16:847-60. 2005
  10. ncbi request reprint Temperature-dependent cell detachment on Pluronic gels
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 6:691-6. 2005

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes
    Seog Jin Seo
    School of Agricultural Biotechnology, Seoul National University, Seoul, Korea
    Tissue Eng 12:33-44. 2006
    ..The results suggest that hepatocytes as stable spheroids enhance liver-specific functions in AL/GC/heparin sponges, providing a new synthetic ECM to design BAL devices...
  2. ncbi request reprint Bioinert surface of pluronic-immobilized flask for preservation of hematopoietic stem cells
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 7:1083-9. 2006
    ..e., low cell binding cultureware). This is caused by the existence of hydrophilic segments of Pluronic F127 on the Pluronic-immobilized flask...
  3. ncbi request reprint Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 1 Kichijoji kitamachi 3, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 78:491-9. 2006
    ..Most purified CD34(+) cells (high recovery ratio of CD34(+) cells divided by recovery ratio of RBCs) were obtained in the recovery process using PU-COOH membranes (pore size, 5.2 microm) at a permeation speed of 0.3-1 mL/min...
  4. ncbi request reprint Temperature-induced cell detachment on immobilized pluronic surface
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 79:380-92. 2006
    ..The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic F127-immobilized flask was significantly higher than that of the cells in polystyrene tissue culture flask...
  5. pmc Visible light regulates neurite outgrowth of nerve cells
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, No 300, Jhongda Rd, Jhongli, Taoyuan, 32001, Taiwan
    Cytotechnology 54:181-8. 2007
    ..Not only suppressed neurite outgrowth but also long connected neurite outgrowths were observed when PC12 cells were cultured under several different wavelengths of light...
  6. ncbi request reprint Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes
    Akon Higuchi
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 85:853-61. 2008
    ..A high recovery ratio of CD34(+) cells was achieved at 4 degrees C in a process using 20% dextran solution through PU membranes having carboxylic acid groups...
  7. ncbi request reprint Cell separation between mesenchymal progenitor cells through porous polymeric membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, Musashino, Tokyo, Japan
    J Biomed Mater Res B Appl Biomater 74:511-9. 2005
    ..Finally, permeation of a solution of human serum albumin through the membrane following filtration of the cells did not result in a separation of cells in the recovery solution...
  8. doi request reprint Preservation of hematopoietic stem and progenitor cells from umbilical cord blood stored in a surface derivatized with polymer nanosegments
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, 32001 Taiwan
    Biomacromolecules 9:634-9. 2008
    ..The foregoing results were attributable to the high density of Pluronic nanosegments on the flask surface, limiting the movement of these flexible segments...
  9. ncbi request reprint Albumin and urea production by hepatocytes cultured on extracellular matrix proteins-conjugated poly(vinyl alcohol) membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomater Sci Polym Ed 16:847-60. 2005
    ..The ECM proteins immobilized on the PVA-ECM membranes contributed not only to the long-term stable production of albumin and urea by hepatocytes, but also the improved surviVal (viability) of hepatocytes on PVA-ECM membranes...
  10. ncbi request reprint Temperature-dependent cell detachment on Pluronic gels
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 6:691-6. 2005
    ..In summary, temperature-dependent detachment of cells from Pluronic gels allows the isolation of cells under mild conditions. This can be a powerful tool for surface marker analysis by flow cytometry...
  11. ncbi request reprint Regulation of neurite outgrowth by intermittent irradiation of visible light
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Phys Chem B 109:11033-6. 2005
    ..The neurite outgrowth ratio was thought to be dependent on the light intensity, the total time of light irradiation in the intermittent light irradiation, and the interval of light irradiation in the intermittent light irradiation...
  12. ncbi request reprint Serum protein adsorption and platelet adhesion on pluronic-adsorbed polysulfone membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 1 Kichijoji kitamachi 3, Musashino, Tokyo 180 8633, Japan
    Biomaterials 24:3235-45. 2003
    ....
  13. doi request reprint Peroxidase activity of DNA aptamer-pt complexes prepared with cisplatin
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan
    J Biomater Sci Polym Ed 21:67-82. 2010
    ..9-times higher sensitivity than DLAA using DNA-Pt complex without fraction. The DNA-Pt complex having specific size was effective for the sensitive detection of thrombin in DLAA...
  14. ncbi request reprint Serum protein adsorption and platelet adhesion on aspartic-acid-immobilized polysulfone membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 1 Kichijoji kitamachi 3, Musashino, Tokyo 180 8633, Japan
    J Biomater Sci Polym Ed 15:1051-63. 2004
    ..In summary, aspartic acid immobilized on the ASP-PSf membranes, which have zwitterions with a net zero charge, effectively contributes to the hydrophilic and hemocompatible sites on the surface of the hydrophobic PSf membranes...
  15. doi request reprint The isolation and differentiation of human adipose-derived stem cells using membrane filtration
    Cheng Han Wu
    Department of Chemical and Materials Engineering, National Central University, No 300, Jhongda Rd, Jhongli, Taoyuan 32001, Taiwan
    Biomaterials 33:8228-39. 2012
    ..These results suggested that the hADSCs with the capability for osteogenic differentiation adhered to the PU membranes...
  16. ncbi request reprint Peripheral blood cell separation through surface-modified polyurethane membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 1 Kichijoji kitamachi 3, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 68:34-42. 2004
    ..The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes...
  17. doi request reprint Effect of the surface density of nanosegments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood
    Li ying Chen
    Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan 32001, Taiwan
    Acta Biomater 8:1749-58. 2012
    ..These data suggest that the specific interaction between HSCs and CS1 helps to maintain the pluripotency of HSCs during the ex vivo expansion of HSCs...
  18. doi request reprint Measurements of movement and diffusion coefficients of single cells on polymeric surface from image analysis
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan
    J Biomater Sci Polym Ed 21:1545-58. 2010
    ....
  19. doi request reprint Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, Taiwan
    Tissue Eng Part A 17:2593-602. 2011
    ....
  20. doi request reprint Microcalorimetrics studies of the thermodynamics and binding mechanism between L-tyrosinamide and aptamer
    Po Hsun Lin
    Institute of Systems Biology and Bioinformatics, National Central University, Jhong Li, Taiwan
    J Phys Chem B 112:6665-73. 2008
    ..In addition, it should be noted that Mg(2+) not only improves binding affinity but also helps change the structure of the DNA aptamer...
  21. ncbi request reprint Synthesis and performance of amphiphilic copolymers for blood cell separation
    Shizue Hayashi Natori
    Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res B Appl Biomater 78:318-26. 2006
    ..The coated filters effectively improved platelet permeation through the filters, with a slight increase in the permeation ratio of leukocytes...
  22. doi request reprint The combined influence of substrate elasticity and surface-grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells
    Suresh S Kumar
    Department of Chemical and Materials Engineering, National Central University, No 300, Jhongli, Taoyuan 32001, Taiwan, ROC Department of Medical Microbiology and Parasitology, Universities Putra Malaysia, Serdang 43400, Slangor, Malaysia
    Biomaterials 34:7632-44. 2013
    ..2-30.4 kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the ex vivo expansion of HSPCs. ..
  23. ncbi request reprint Photon-modulated changes of cell attachments on poly(spiropyran-co-methyl methacrylate) membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 5:1770-4. 2004
    ..Light-induced detachment of fibrinogen adsorbed on poly(NSP-co-MMA) coated glass plates was also observed in this investigation...
  24. ncbi request reprint Production of interferon-beta by fibroblast cells on membranes prepared with RGD-containing peptides
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, Musashino 180 8633, Japan
    J Biomed Mater Res A 65:369-78. 2003
    ..We suggest that the cells must bind to the RGD sequence having the appropriate conformation through their integrin beta(1) for enhanced production of interferon-beta...
  25. ncbi request reprint Visible light is able to regulate neurite outgrowth
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomater Sci Polym Ed 14:1377-88. 2003
    ..These results hold promise for the creation of patterned neuronal networks corresponding to patterned irradiation of visible light on nerve cells...
  26. ncbi request reprint Novel enzymatic properties of DNA-Pt complexes
    Yuki Matsuoka
    Department of Materials and Life Science, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    Biomacromolecules 8:2684-8. 2007
    ..e., [A]20, [G]20, [C]20, [T]20, and [AG]10) exhibited peroxidase enzymatic activity. These complexes showed good thermal stability as compared to native horseradish peroxidase...
  27. doi request reprint Preparation of a DNA aptamer-Pt complex and its use in the colorimetric sensing of thrombin and anti-thrombin antibodies
    Akon Higuchi
    Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, 32001 Taiwan
    Anal Chem 80:6580-6. 2008
    ..DLAAs having high thermal stability were developed using these complexes, which were free of animal and plant matter because neither antibodies nor horseradish peroxidase were used in their synthesis...
  28. ncbi request reprint Cell separation of hepatocytes and fibroblasts through surface-modified polyurethane membranes
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, 3 3 1 Kichijoji Kitamachi, Musashino, Tokyo 180 8633, Japan
    J Biomed Mater Res A 71:470-9. 2004
    ..The separation of hepatocytes isolated from mice at different ages was also accomplished by permeating the cell mixture through unmodified and surface-modified PU membranes...
  29. ncbi request reprint Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins
    Mariko Hara
    Department of Applied Chemistry, Seikei University, Musashino 180 8633, Japan
    J Biomater Sci Polym Ed 14:139-55. 2003
    ..These findings indicate that cells tend to attach to the surface by secreting ECM proteins such as FN when they are grown on substrates that provide weak cell attachment...
  30. doi request reprint Investigation of the mechanism of beta-amyloid fibril formation by kinetic and thermodynamic analyses
    Ming Shen Lin
    Department of Chemical and Materials Engineering, National Central University, Jhong Li, Taiwan 320
    Langmuir 24:5802-8. 2008
    ..Our study provides an insight into the discriminative mechanisms of beta-amyloid aggregation via kinetics and thermodynamics, especially the first reported thermodynamics information obtained by ITC...
  31. pmc Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells
    Hsin Chung Lee
    Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taoyuan
    Drug Des Devel Ther 7:491-502. 2013
    ..The aim of this study was to evaluate whether the drug-resistant cells isolated in this study were cancer-initiating cells, known also as cancer stem cells (CSCs)...
  32. ncbi request reprint Chemically modified polysulfone hollow fibers with vinylpyrrolidone having improved blood compatibility
    Akon Higuchi
    Department of Applied Chemistry, Seikei University, Musashino, Tokyo, Japan
    Biomaterials 23:2659-66. 2002
    ....
  33. doi request reprint A highly stable nonbiofouling surface with well-packed grafted zwitterionic polysulfobetaine for plasma protein repulsion
    Yung Chang
    R and D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong Li, Taoyuan 320, Taiwan
    Langmuir 24:5453-8. 2008
    ..This work further concludes that a surface with good hemocompatibility can be achieved by the well-packed surface-grafted polySBMA brushes...
  34. ncbi request reprint Difference in the attachment of hepatocytes between a poly(gamma-benzyl L-glutamate) (PBLG)/poly(N-isopropylacrylamide) (PNIPAAm) diblock copolymer cast surface and a PBlg/PNIPAAm Langmuir-Blodgett one
    Shojiro Shimojo
    Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan
    J Biomater Sci Polym Ed 13:829-41. 2002
    ..Also, the spheroid formation of the hepatocytes increased with an increase of the PNIPAAm content in the block copolymer cast films...
  35. ncbi request reprint Dehydration of an ethanol/water azeotrope by novel organic-inorganic hybrid membranes based on quaternized chitosan and tetraethoxysilane
    Tadashi Uragami
    Faculty of Engineering and High Technology Research Center, Kansai University, Suita, Osaka 564 8680, Japan
    Biomacromolecules 5:1567-74. 2004
    ..The relationship between the structure of q-Chito/TEOS hybrid membranes and their permeation and separation characteristics during pervaporation of an ethanol/water azeotrope is discussed in detail...