Ohtake F, Tsuchiya H, Tanaka K, Saeki Y. Methods to measure ubiquitin chain length and linkage. Methods Enzymol. 2019;618:105-133 pubmed publisher
..These strategies will contribute to our knowledge of polymeric ubiquitin signals and permit investigation of new mechanisms concerning the ubiquitin code. ..
Tsuchiya H, Ohtake F, Arai N, Kaiho A, Yasuda S, Tanaka K, et al
. In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome. Mol Cell. 2017;66:488-502.e7 pubmed publisher
..Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome. ..
Tsuchiya H, Burana D, Ohtake F, Arai N, Kaiho A, Komada M, et al
. Ub-ProT reveals global length and composition of protein ubiquitylation in cells. Nat Commun. 2018;9:524 pubmed publisher
..Thus, the Ub-ProT method can contribute to our understanding of mechanisms regulating physiological ubiquitin chain lengths and composition. ..