Y Takaki

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. ncbi request reprint Biochemical identification of the neutral endopeptidase family member responsible for the catabolism of amyloid beta peptide in the brain
    Y Takaki
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, Wako, Saitama 351 0198, Japan
    J Biochem 128:897-902. 2000
  2. ncbi request reprint Metabolic regulation of brain Abeta by neprilysin
    N Iwata
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, Wako shi 351 0198 Japan
    Science 292:1550-2. 2001
  3. ncbi request reprint Neprilysin degrades both amyloid beta peptides 1-40 and 1-42 most rapidly and efficiently among thiorphan- and phosphoramidon-sensitive endopeptidases
    K Shirotani
    Laboratory for Proteolytic Neuroscience, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 276:21895-901. 2001
  4. ncbi request reprint Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus
    H J Lee
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako Shi, 351 0198, Saitama, Japan
    Biochim Biophys Acta 1493:273-8. 2000
  5. ncbi request reprint Cloning and expression of the gene encoding RNA polymerase alpha subunit from alkaliphilic Bacillus sp. strain C-125
    K Nakasone
    Deep Star Group, Japan Marine Science and Technology Center, Kanagawa, Japan
    FEMS Microbiol Lett 168:269-76. 1998
  6. ncbi request reprint Direct interaction of the beta-domain of VHL tumor suppressor protein with the regulatory domain of atypical PKC isotypes
    H Okuda
    Department of Urology, Kochi Medical School, Kochi, 783 8505, Japan
    Biochem Biophys Res Commun 263:491-7. 1999
  7. ncbi request reprint The von Hippel-Lindau tumor suppressor protein mediates ubiquitination of activated atypical protein kinase C
    H Okuda
    Department of Urology, Kochi Medical School, Kochi 783 8505, Japan
    J Biol Chem 276:43611-7. 2001

Collaborators

Detail Information

Publications7

  1. ncbi request reprint Biochemical identification of the neutral endopeptidase family member responsible for the catabolism of amyloid beta peptide in the brain
    Y Takaki
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, Wako, Saitama 351 0198, Japan
    J Biochem 128:897-902. 2000
    ..Furthermore, kinetic analysis, giving a K(m) value of 2.8 +/- 0.76 microM, indicated that Abeta(1-42) is a relevant substrate for neprilysin...
  2. ncbi request reprint Metabolic regulation of brain Abeta by neprilysin
    N Iwata
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, Wako shi 351 0198 Japan
    Science 292:1550-2. 2001
    ..Our observations suggest that even partial down-regulation of neprilysin activity, which could be caused by aging, can contribute to AD development by promoting Abeta accumulation...
  3. ncbi request reprint Neprilysin degrades both amyloid beta peptides 1-40 and 1-42 most rapidly and efficiently among thiorphan- and phosphoramidon-sensitive endopeptidases
    K Shirotani
    Laboratory for Proteolytic Neuroscience, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 276:21895-901. 2001
    ..Therefore, manipulating the activity of NEP would be a useful approach in regulating Abeta levels in the brain...
  4. ncbi request reprint Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus
    H J Lee
    Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako Shi, 351 0198, Saitama, Japan
    Biochim Biophys Acta 1493:273-8. 2000
    ..Rat aminopeptidase A mRNAs were detected in the kidney, liver, heart and brain by Northern blot analysis. When overexpressed in COS-1 cells, APAL shows apparent aminopeptidase A activity, whereas APAS does not...
  5. ncbi request reprint Cloning and expression of the gene encoding RNA polymerase alpha subunit from alkaliphilic Bacillus sp. strain C-125
    K Nakasone
    Deep Star Group, Japan Marine Science and Technology Center, Kanagawa, Japan
    FEMS Microbiol Lett 168:269-76. 1998
    ..coli cells and purified to near homogeneity...
  6. ncbi request reprint Direct interaction of the beta-domain of VHL tumor suppressor protein with the regulatory domain of atypical PKC isotypes
    H Okuda
    Department of Urology, Kochi Medical School, Kochi, 783 8505, Japan
    Biochem Biophys Res Commun 263:491-7. 1999
    ..Since aPKC isotypes have been implicated in the regulation of cell growth and apoptosis, these results suggest that aPKC isotypes are potential direct target of the VHL beta-domain...
  7. ncbi request reprint The von Hippel-Lindau tumor suppressor protein mediates ubiquitination of activated atypical protein kinase C
    H Okuda
    Department of Urology, Kochi Medical School, Kochi 783 8505, Japan
    J Biol Chem 276:43611-7. 2001
    ..Given the known function of aPKC in the regulation of cell polarity and cell growth, PKClambda may be a target of pVHL in its function as a tumor suppressor...