K Sugasawa

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. ncbi request reprint Signal transducer and activator of transcription 3 is a key regulator of keratinocyte survival and proliferation following UV irradiation
    Shigetoshi Sano
    Department of Dermatology and Social, Osaka University Graduate School of Medicine, Japan
    Cancer Res 65:5720-9. 2005
  2. pmc Two human homologs of Rad23 are functionally interchangeable in complex formation and stimulation of XPC repair activity
    K Sugasawa
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Mol Cell Biol 17:6924-31. 1997
  3. ncbi request reprint Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair
    K Sugasawa
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Mol Cell 2:223-32. 1998
  4. pmc A multistep damage recognition mechanism for global genomic nucleotide excision repair
    K Sugasawa
    Cellular Physiology Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama 351 0198, Japan
    Genes Dev 15:507-21. 2001
  5. ncbi request reprint A molecular mechanism for DNA damage recognition by the xeroderma pigmentosum group C protein complex
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, 351 0198, Saitama, Japan
    DNA Repair (Amst) 1:95-107. 2002
  6. pmc XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes
    P J van der Spek
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Nucleic Acids Res 24:2551-9. 1996
  7. pmc Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23
    C Masutani
    Cellular Physiology Laboratory, Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    EMBO J 13:1831-43. 1994
  8. ncbi request reprint The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNA
    M Yokoi
    Cellular Physiology Laboratory, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 275:9870-5. 2000
  9. ncbi request reprint Novel functional interactions between nucleotide excision DNA repair proteins influencing the enzymatic activities of TFIIH, XPG, and ERCC1-XPF
    G S Winkler
    Department of Cell Biology and Genetics, Medical Genetics Center, Center of Biomedical Genetics, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
    Biochemistry 40:160-5. 2001
  10. ncbi request reprint Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23
    P J van der Spek
    Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands
    Genomics 23:651-8. 1994

Collaborators

  • Katsuhito Kino
  • Claire Wyman
  • J H Hoeijmakers
  • Ryotaro Nishi
  • S Iwai
  • Fumio Yatagai
  • Masamitsu Honma
  • Y Ohkuma
  • T Okamoto
  • T Enomoto
  • Fumio Hanaoka
  • K Tanaka
  • H B Beverloo
  • J Kros
  • Hisao Masai
  • G Lipps
  • Jessica M Y Ng
  • Yuichiro Shimizu
  • Toshio Mori
  • Steven Bergink
  • Wim Vermeulen
  • Gijsbertus T J van der Horst
  • Gentaro Yasuda
  • Shigetoshi Sano
  • Daichi Baba
  • Takeshi Yasuda
  • Masahiro Shirakawa
  • Jun Goo Jee
  • Nils Wijgers
  • Hidekazu Hiroaki
  • Hidehito Tochio
  • Kenichiro Fujiwara
  • C Masutani
  • Yuki Okuda
  • Masayuki Yokoi
  • Zhiying You
  • Ana Janićijević
  • Harry Vrieling
  • P J van der Spek
  • Akio Uchida
  • Thomas Hey
  • G S Winkler
  • M Yokoi
  • Eriko Watanabe
  • Miria Stefanini
  • Donata Orioli
  • Ben A Oostra
  • John van Swieten
  • Rob Willemsen
  • Humaira Yousaf
  • Lies Anne Severijnen
  • Satoshi Takagi
  • Nobuo Maita
  • Satoshi Itami
  • Junji Takeda
  • Yasuhiro Uchimura
  • Masahiro Kira
  • Ken Kataoka
  • Kaoru Kiguchi
  • Keith Syson Chan
  • Tadahiro Shiomi
  • Masahito Tarutani
  • Hisato Saitoh
  • John DiGiovanni
  • Chojiro Kojima
  • Takeshi Tenno
  • Izuru Ohki
  • Miodrag Djurica
  • Takeshi Mizuno
  • Yukio Ishimi
  • Chikahide Masutani
  • Naoshi Dohmae
  • Marito Araki
  • J Anton Grootegoed
  • Marja P Ooms
  • Jan T M Vreeburg
  • Theo G M F Gorgels
  • Pim Visser
  • Gerhard Krauss
  • Rudolph B Beems
  • A P Eker
  • W L de Laat
  • D Bootsma
  • T Maekawa
  • S Rademakers
  • A Eker
  • C Visser
  • E M Smit
  • T Sonoyama
  • A Hagemeijer

Detail Information

Publications36

  1. ncbi request reprint Signal transducer and activator of transcription 3 is a key regulator of keratinocyte survival and proliferation following UV irradiation
    Shigetoshi Sano
    Department of Dermatology and Social, Osaka University Graduate School of Medicine, Japan
    Cancer Res 65:5720-9. 2005
    ..These data suggest that Stat3 is required for survival and proliferation of keratinocytes following UVB exposure and that Stat3 is tightly regulated as part of a novel protective mechanism against UVB-induced skin cancer...
  2. pmc Two human homologs of Rad23 are functionally interchangeable in complex formation and stimulation of XPC repair activity
    K Sugasawa
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Mol Cell Biol 17:6924-31. 1997
    ..Furthermore, both rhHR23 proteins function in a defined NER system reconstituted with purified proteins, indicating direct involvement of hHR23 proteins in the DNA repair reaction via interaction with XPC...
  3. ncbi request reprint Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair
    K Sugasawa
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Mol Cell 2:223-32. 1998
    ..After this subpathway-specific initial lesion detection, XPA may function as a common damage verifier and adaptor to the core of the NER apparatus...
  4. pmc A multistep damage recognition mechanism for global genomic nucleotide excision repair
    K Sugasawa
    Cellular Physiology Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama 351 0198, Japan
    Genes Dev 15:507-21. 2001
    ..A multistep mechanism of this sort may provide a molecular basis for ensuring the high level of damage discrimination that is required for global genomic NER...
  5. ncbi request reprint A molecular mechanism for DNA damage recognition by the xeroderma pigmentosum group C protein complex
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, 351 0198, Saitama, Japan
    DNA Repair (Amst) 1:95-107. 2002
    ..The specific binding of XPC-HR23B is undoubtedly an important molecular process, based on which NER machinery detects a wide variety of lesions that vary in terms of chemical structure during DNA repair...
  6. pmc XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes
    P J van der Spek
    Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands
    Nucleic Acids Res 24:2551-9. 1996
    ..Consistent with a function in repair or DNA/chromatin metabolism, immunofluorescence studies show all XPC, HHR23B and (the free) HHR23A to reside in the nucleus...
  7. pmc Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23
    C Masutani
    Cellular Physiology Laboratory, Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    EMBO J 13:1831-43. 1994
    ..All RAD23 derivatives share a ubiquitin-like N-terminus. The nature of the XP-C defect implies that the complex exerts a unique function in the genome-overall repair pathway which is important for prevention of skin cancer...
  8. ncbi request reprint The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNA
    M Yokoi
    Cellular Physiology Laboratory, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 275:9870-5. 2000
    ..We conclude that the XPC-HR23B protein complex plays a crucial role in the recruitment of TFIIH to damaged DNA in global genome repair...
  9. ncbi request reprint Novel functional interactions between nucleotide excision DNA repair proteins influencing the enzymatic activities of TFIIH, XPG, and ERCC1-XPF
    G S Winkler
    Department of Cell Biology and Genetics, Medical Genetics Center, Center of Biomedical Genetics, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
    Biochemistry 40:160-5. 2001
    ..These results point toward additional roles for TFIIH and ATP during NER distinct from a requirement for DNA unwinding in the regulation of the endonuclease activities of XPG and ERCC1-XPF...
  10. ncbi request reprint Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23
    P J van der Spek
    Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands
    Genomics 23:651-8. 1994
    ..1. Pulsed-field gel electrophoresis revealed that the HHR23B and XPC genes possibly share a MluI restriction fragment of about 625 kb. Potential involvement of the HHR23 genes in human genetic disorders is discussed...
  11. ncbi request reprint Mutagenic radioadaptation in a human lymphoblastoid cell line
    Fumio Yatagai
    Advanced Development and Support Center, The Institute of Physical and Chemical Research RIKEN, Saitama 351 0198, Japan
    Mutat Res 638:48-55. 2008
    ..1 x 10(-6)), reflecting an enhancement in HR repair of DNA double-strand breaks. Our findings suggest that the radioadaptive response in TK6 cells follows mainly from mutations at the base-sequence level, not the chromosome level...
  12. ncbi request reprint Nucleosomal structure of undamaged DNA regions suppresses the non-specific DNA binding of the XPC complex
    Takeshi Yasuda
    Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Saitama 351 0198, Japan
    DNA Repair (Amst) 4:389-95. 2005
    ....
  13. ncbi request reprint Nucleotide excision repair of 5-formyluracil in vitro is enhanced by the presence of mismatched bases
    Katsuhito Kino
    Cellular Physiology Laboratory, RIKEN, Japan Science and Technology Corporation, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochemistry 43:2682-7. 2004
    ..Therefore, it is concluded that even fU, regarded as a shape mimic of thymine, can be recognized as a substrate of NER incision, and the efficiency depends on instability of the base pair...
  14. ncbi request reprint UV-induced ubiquitylation of XPC complex, the UV-DDB-ubiquitin ligase complex, and DNA repair
    Kaoru Sugasawa
    Genome Damage Response Research Unit, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Mol Histol 37:189-202. 2006
    ....
  15. ncbi request reprint The xeroderma pigmentosum group C protein complex and ultraviolet-damaged DNA-binding protein: functional assays for damage recognition factors involved in global genome repair
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Hirosawa, Wako, Saitama, Japan
    Methods Enzymol 408:171-88. 2006
    ..This ubiquitylation is mediated by ubiquitin ligase associated with UV-DDB and is important for the NER process of UV-induced lesions. Methods for detecting the UV-DDB-dependent ubiquitylation in vivo and in vitro are also described...
  16. pmc Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein
    Ryotaro Nishi
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Mol Cell Biol 25:5664-74. 2005
    ..These results reveal a novel vital function for centrin 2 in NER, the potentiation of damage recognition by XPC...
  17. ncbi request reprint Relative levels of the two mammalian Rad23 homologs determine composition and stability of the xeroderma pigmentosum group C protein complex
    Yuki Okuda
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    DNA Repair (Amst) 3:1285-95. 2004
    ....
  18. pmc Xeroderma pigmentosum group C protein interacts physically and functionally with thymine DNA glycosylase
    Yuichiro Shimizu
    Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Japan Science and Technology Corporation, Wako, Saitama
    EMBO J 22:164-73. 2003
    ....
  19. pmc A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein
    Jessica M Y Ng
    MGC Department of Cell Biology and Genetics, Centre for Biomedical Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
    Genes Dev 17:1630-45. 2003
    ..These findings resolve the primary function of RAD23 in repair and reveal a novel DNA-damage-dependent regulation mechanism of DNA repair in eukaryotes, which may be part of a more global damage-response circuitry...
  20. pmc Developmental defects and male sterility in mice lacking the ubiquitin-like DNA repair gene mHR23B
    Jessica M Y Ng
    MGC Department of Cell Biology and Genetics, Centre for Biomedical Genetics, Erasmus University Rotterdam, Rotterdam, The Netherlands
    Mol Cell Biol 22:1233-45. 2002
    ..This function may involve regulation of protein stability via the ubiquitin/proteasome pathway and is not or only in part compensated for by mHR23A...
  21. ncbi request reprint An approach to estimate radioadaptation from DSB repair efficiency
    Fumio Yatagai
    Metallomics Imaging Research Unit, Center for Molecular Imaging Science, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    J Radiat Res 50:407-13. 2009
    ..We suggest that the present evaluation of DSB repair using this I-SceI system, may contribute to our overall understanding of radioadaptation...
  22. ncbi request reprint The DNA repair-ubiquitin-associated HR23 proteins are constituents of neuronal inclusions in specific neurodegenerative disorders without hampering DNA repair
    Steven Bergink
    MGC CBG Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000DR, Rotterdam, The Netherlands
    Neurobiol Dis 23:708-16. 2006
    ..This illustrates that impairment of the ubiquitin-proteasome system (UPS) by expanded glutamine repeats, including the sequestration of HR23B, is not affecting NER...
  23. ncbi request reprint UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex
    Kaoru Sugasawa
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Japan Science and Technology Agency, Wako, Saitama 351 0198, Japan
    Cell 121:387-400. 2005
    ..Our results strongly suggest that ubiquitylation plays a critical role in the transfer of the UV-induced lesion from UV-DDB to XPC...
  24. ncbi request reprint DNA bending by the human damage recognition complex XPC-HR23B
    Ana Janićijević
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR, Rotterdam, The Netherlands
    DNA Repair (Amst) 2:325-36. 2003
    ..We discuss the importance of the XPC-HR23B-induced distortion as an architectural feature that can be exploited for subsequent assembly of an active NER complex...
  25. pmc In vivo destabilization and functional defects of the xeroderma pigmentosum C protein caused by a pathogenic missense mutation
    Gentaro Yasuda
    Biosignal Research Center, Kobe University, 1 1 Rokkodai, Kobe, Hyogo, Japan
    Mol Cell Biol 27:6606-14. 2007
    ..As well as highlighting the importance of UV-DDB in recruiting XPC to UV-damaged sites, these findings demonstrate the role of DNA binding by XPC in the assembly of subsequent NER intermediate complexes...
  26. doi request reprint Xeroderma pigmentosum genes: functions inside and outside DNA repair
    Kaoru Sugasawa
    Biosignal Research Center, Organization of Advanced Science and Technology, Kobe University and SORST, Japan Science and Technology Agency, 1 1 Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Carcinogenesis 29:455-65. 2008
    ..Such differential functions not only explain clinical heterogeneity among different genetic complementation groups but also have implications for the promotion of carcinogenic processes in XP patients...
  27. ncbi request reprint [Roles for ubiquitylation in DNA repair]
    Kaoru Sugasawa
    Tanpakushitsu Kakusan Koso 52:760-7. 2007
  28. ncbi request reprint [Repair mechanism of UV-induced DNA lesions]
    Ryotaro Nishi
    Tanpakushitsu Kakusan Koso 51:2126-33. 2006
  29. ncbi request reprint Sensing of DNA damage by XPC/Rad4: one protein for many lesions
    Kaoru Sugasawa
    Nat Struct Mol Biol 14:887-8. 2007
  30. ncbi request reprint [DNA repair pathways involving Cul4A ubiquitin ligases]
    Kaoru Sugasawa
    Tanpakushitsu Kakusan Koso 51:1339-44. 2006
  31. ncbi request reprint The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assay
    Thomas Hey
    Lehrstuhl für Biochemie, Universitat Bayreuth, Universitatsstrasse 30, 95447 Bayreuth, Germany
    Biochemistry 41:6583-7. 2002
    ..Upon addition of XPA to the XPC binding reaction mixtures, it was not possible to detect cooperative ternary complex formation on the platinated 36 bp probe...
  32. ncbi request reprint [Repair mechanism of DNA base lesions induced by ultraviolet light irradiation]
    Kaoru Sugasawa
    Genome Damage Response Research Unit, RIKEN Discovery Research Institute, Japan
    Seikagaku 78:516-21. 2006
  33. ncbi request reprint The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH
    Akio Uchida
    Institute for Molecular and Cellular Biology, Osaka University, 1 3 Yamada oka, Suita, 565 0871, Osaka, Japan
    DNA Repair (Amst) 1:449-61. 2002
    ..These results suggest that following initial damage recognition, the carboxy terminus of XPC may be essential for the recruitment of TFIIH, and that most truncation mutations identified in XP-C patients result in non-functional proteins...
  34. ncbi request reprint Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B
    Kenichiro Fujiwara
    Graduate School of Integrated Science, Yokohama City University, 1 7 29 Suehiro, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    J Biol Chem 279:4760-7. 2004
    ..The pH-dependent protonation of this residue interferes with the access of ubiquitin to the UIM and the ubiquitin-associated domain (UBA), and its mutation to a smaller residue increases the affinity of ubiquitin for UIM...
  35. ncbi request reprint Crystal structure of thymine DNA glycosylase conjugated to SUMO-1
    Daichi Baba
    Graduate School of Integrated Science, Yokohama City University, Yokohama 230 0045, Japan
    Nature 435:979-82. 2005
    ..This helix is formed by covalent and non-covalent contacts between TDG and SUMO-1. The non-covalent contacts are also essential for release from the product DNA, as verified by mutagenesis...
  36. pmc Thymine-rich single-stranded DNA activates Mcm4/6/7 helicase on Y-fork and bubble-like substrates
    Zhiying You
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
    EMBO J 22:6148-60. 2003
    ..These findings lead us to propose that selective activation by stretches of thymine sequences of a fraction of Mcm helicases loaded onto chromatin may be the determinant for selection of initiation sites on mammalian genomes...