Y Ohtsubo

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. ncbi request reprint BphS, a key transcriptional regulator of bph genes involved in polychlorinated biphenyl/biphenyl degradation in Pseudomonas sp. KKS102
    Y Ohtsubo
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 276:36146-54. 2001
  2. pmc Novel approach to the improvement of biphenyl and polychlorinated biphenyl degradation activity: promoter implantation by homologous recombination
    Yoshiyuki Ohtsubo
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 69:146-53. 2003
  3. ncbi request reprint The dual functions of biphenyl-degrading ability of Pseudomonas sp. KKS102: energy acquisition and substrate detoxification
    Mina Delawary
    Department of Biotechnology, University of Tokyo, Japan
    Biosci Biotechnol Biochem 67:1970-5. 2003
  4. pmc Degradation of beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26
    Yuji Nagata
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 71:2183-5. 2005
  5. ncbi request reprint Identification of a response regulator gene for catabolite control from a PCB-degrading beta-proteobacteria, Acidovorax sp. KKS102
    Yoshiyuki Ohtsubo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, 2 1 1 Katahira, Sendai 980 8577, Japan
    Mol Microbiol 60:1563-75. 2006
  6. doi request reprint Insertion sequence-based cassette PCR: cultivation-independent isolation of gamma-hexachlorocyclohexane-degrading genes from soil DNA
    Genki Fuchu
    Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Appl Microbiol Biotechnol 79:627-32. 2008
  7. ncbi request reprint Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements
    Hirokazu Yano
    Graduate School of Life Sciences, Tohoku University, Katahira, Sendai 980 8577, Japan
    J Mol Biol 369:11-26. 2007
  8. pmc Complete nucleotide sequence of an exogenously isolated plasmid, pLB1, involved in gamma-hexachlorocyclohexane degradation
    Ryo Miyazaki
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 72:6923-33. 2006
  9. ncbi request reprint Distribution of gamma-hexachlorocyclohexane-degrading genes on three replicons in Sphingobium japonicum UT26
    Yuji Nagata
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    FEMS Microbiol Lett 256:112-8. 2006
  10. ncbi request reprint Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616
    Yuji Nagata
    Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    J Biosci Bioeng 99:603-10. 2005

Collaborators

  • Y Nagata
  • Keisuke Miyauchi
  • Masahiro Sota
  • Hiroyucki Sawada
  • Masao Fukuda
  • Masataka Tsuda
  • Ryo Miyazaki
  • Yukari Sato
  • Satoshi Yuhara
  • Genki Fuchu
  • Michihiro Ito
  • Hirokazu Yano
  • Ryo Endo
  • Mina Delawary
  • Hiroyuki Goto
  • Harunobu Komatsu
  • Akira Ono
  • Christine E Garruto
  • Peter A Williams
  • Gerben J Zylstra
  • Marta Monincov√°
  • Jiri Damborsky
  • Radka Chaloupkova
  • Mayuko Kamakura
  • Zbynek Prokop
  • Kiwamu Minamisawa
  • Akinori Ohta

Detail Information

Publications15

  1. ncbi request reprint BphS, a key transcriptional regulator of bph genes involved in polychlorinated biphenyl/biphenyl degradation in Pseudomonas sp. KKS102
    Y Ohtsubo
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 276:36146-54. 2001
    ..We concluded that the negative regulator BphS plays a central role in the regulation of bph gene expression through its action at the pE promoter...
  2. pmc Novel approach to the improvement of biphenyl and polychlorinated biphenyl degradation activity: promoter implantation by homologous recombination
    Yoshiyuki Ohtsubo
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 69:146-53. 2003
    ..This strategy removes the rate-limiting factor associated with transcription and has the potential to improve the degradation activity of a wide variety of microorganisms involved in biodegradation...
  3. ncbi request reprint The dual functions of biphenyl-degrading ability of Pseudomonas sp. KKS102: energy acquisition and substrate detoxification
    Mina Delawary
    Department of Biotechnology, University of Tokyo, Japan
    Biosci Biotechnol Biochem 67:1970-5. 2003
    ....
  4. pmc Degradation of beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26
    Yuji Nagata
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 71:2183-5. 2005
    ..We demonstrate here that the haloalkane dehalogenase LinB, reported earlier to mediate the second step in the degradation of gamma-HCH in Sphingomonas paucimobilis UT26, metabolizes beta-HCH to produce 2,3,4,5,6-pentachlorocyclohexanol...
  5. ncbi request reprint Identification of a response regulator gene for catabolite control from a PCB-degrading beta-proteobacteria, Acidovorax sp. KKS102
    Yoshiyuki Ohtsubo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, 2 1 1 Katahira, Sendai 980 8577, Japan
    Mol Microbiol 60:1563-75. 2006
    ..The genes highly similar to bphQ were found from several beta-proteobacteria, such as Burkholderia cenocepacia J2315, B. multivorans ATCC17616, B. xenovorans LB400 and Ralstonia solanacearum RS1085...
  6. doi request reprint Insertion sequence-based cassette PCR: cultivation-independent isolation of gamma-hexachlorocyclohexane-degrading genes from soil DNA
    Genki Fuchu
    Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Appl Microbiol Biotechnol 79:627-32. 2008
    ....
  7. ncbi request reprint Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements
    Hirokazu Yano
    Graduate School of Life Sciences, Tohoku University, Katahira, Sendai 980 8577, Japan
    J Mol Biol 369:11-26. 2007
    ....
  8. pmc Complete nucleotide sequence of an exogenously isolated plasmid, pLB1, involved in gamma-hexachlorocyclohexane degradation
    Ryo Miyazaki
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 72:6923-33. 2006
    ..To the best of our knowledge, this is the first detailed report of a plasmid involved in gamma-HCH degradation...
  9. ncbi request reprint Distribution of gamma-hexachlorocyclohexane-degrading genes on three replicons in Sphingobium japonicum UT26
    Yuji Nagata
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    FEMS Microbiol Lett 256:112-8. 2006
    ..The linF gene encoding maleylacetate reductase was located on Chr II. These results indicated that the genes for the complete gamma-hexachlorocyclohexane degradation are dispersed on the three large replicons of UT26...
  10. ncbi request reprint Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616
    Yuji Nagata
    Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    J Biosci Bioeng 99:603-10. 2005
    ..Pulsed-field gel electrophoresis and Southern blot analyses indicated that both the dnaA and dnaK gene regions exist as single copies on the 3.4 Mb chromosome...
  11. pmc Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, encode haloalkane dehalogenases with novel structures and substrate specificities
    Yukari Sato
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 71:4372-9. 2005
    ..The dehalogenase activity of resting cells and Northern blot analyses both revealed that the dmlA and dbjA genes were expressed under normal culture conditions in MAFF303099 and USDA110 strain cells, respectively...
  12. pmc High-temperature-induced transposition of insertion elements in burkholderia multivorans ATCC 17616
    Yoshiyuki Ohtsubo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Appl Environ Microbiol 71:1822-8. 2005
    ..The efficient experimental protocol developed in this study will be useful in quantitatively and simultaneously investigating various IS elements, as well as in capturing novel functional mobile elements from a wide variety of bacteria...
  13. pmc Identification and characterization of genes involved in the downstream degradation pathway of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis UT26
    Ryo Endo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai, 980 8577, Japan
    J Bacteriol 187:847-53. 2005
    ..Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for gamma-HCH utilization...
  14. ncbi request reprint Strategies for bioremediation of polychlorinated biphenyls
    Yoshiyuki Ohtsubo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai, 980 8577, Japan
    Appl Microbiol Biotechnol 65:250-8. 2004
    ..In this mini-review, we briefly summarize these strategies and provide potentially useful knowledge for the further improvement of the bacterial breakdown of PCBs...
  15. doi request reprint Pleiotropic roles of iron-responsive transcriptional regulator Fur in Burkholderia multivorans
    Satoshi Yuhara
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2 1 1 Katahira, Sendai 980 8577, Japan
    Microbiology 154:1763-74. 2008
    ..Nine of the 13 regions were presumed to be involved in the acquisition and utilization of iron...