Hitoshi Niwa

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. doi request reprint Mouse ES cell culture system as a model of development
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, Kobe, Hyogo, Japan
    Dev Growth Differ 52:275-83. 2010
  2. pmc E-cadherin promotes incorporation of mouse epiblast stem cells into normal development
    Satoshi Ohtsuka
    Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology CDB, Kobe, Japan
    PLoS ONE 7:e45220. 2012
  3. pmc Esrrb is a pivotal target of the Gsk3/Tcf3 axis regulating embryonic stem cell self-renewal
    Graziano Martello
    Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge UK
    Cell Stem Cell 11:491-504. 2012
  4. pmc Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data
    Alexei A Sharov
    Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    BMC Genomics 9:269. 2008
  5. doi request reprint Platypus Pou5f1 reveals the first steps in the evolution of trophectoderm differentiation and pluripotency in mammals
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Evol Dev 10:671-82. 2008
  6. ncbi request reprint A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Nature 460:118-22. 2009
  7. ncbi request reprint Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
  8. ncbi request reprint [Rivalry between transcription factors to determine cell fates in mammalian pre implantation development]
    Hitoshi Niwa
    Tanpakushitsu Kakusan Koso 51:216-28. 2006
  9. ncbi request reprint [Definitions of key words in stem cell biology]
    Hitoshi Niwa
    Tanpakushitsu Kakusan Koso 51:1610-7. 2006
  10. ncbi request reprint Open conformation chromatin and pluripotency
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Genes Dev 21:2671-6. 2007

Detail Information

Publications63

  1. doi request reprint Mouse ES cell culture system as a model of development
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, Kobe, Hyogo, Japan
    Dev Growth Differ 52:275-83. 2010
    ..For these reasons, mES cells can be regarded as a useful tool for analyzing molecular mechanisms underlying early mouse development...
  2. pmc E-cadherin promotes incorporation of mouse epiblast stem cells into normal development
    Satoshi Ohtsuka
    Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology CDB, Kobe, Japan
    PLoS ONE 7:e45220. 2012
    ....
  3. pmc Esrrb is a pivotal target of the Gsk3/Tcf3 axis regulating embryonic stem cell self-renewal
    Graziano Martello
    Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge UK
    Cell Stem Cell 11:491-504. 2012
    ..These findings highlight a key role for Esrrb in regulating the naive pluripotent state and illustrate compensation among the core pluripotency factors...
  4. pmc Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data
    Alexei A Sharov
    Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    BMC Genomics 9:269. 2008
    ..However, many responding genes with binding sites may not be direct targets because response may be mediated by other genes and ChIP-binding site may not be functional in terms of transcription regulation...
  5. doi request reprint Platypus Pou5f1 reveals the first steps in the evolution of trophectoderm differentiation and pluripotency in mammals
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Evol Dev 10:671-82. 2008
    ....
  6. ncbi request reprint A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Nature 460:118-22. 2009
    ....
  7. ncbi request reprint Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
    ..This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development...
  8. ncbi request reprint [Rivalry between transcription factors to determine cell fates in mammalian pre implantation development]
    Hitoshi Niwa
    Tanpakushitsu Kakusan Koso 51:216-28. 2006
  9. ncbi request reprint [Definitions of key words in stem cell biology]
    Hitoshi Niwa
    Tanpakushitsu Kakusan Koso 51:1610-7. 2006
  10. ncbi request reprint Open conformation chromatin and pluripotency
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Genes Dev 21:2671-6. 2007
  11. ncbi request reprint How is pluripotency determined and maintained?
    Hitoshi Niwa
    RIKEN Center for Developmental Biology CDB Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7 5 1 Kusunokicho, Chuo Ku, Kobe, Hyogo 6500017, Japan
    Development 134:635-46. 2007
    ....
  12. ncbi request reprint Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells
    Shinji Masui
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chu o ku, Kobe, Hyogo 650 0047, Japan
    Nat Cell Biol 9:625-35. 2007
    ..These results indicate that the essential function of Sox2 is to stabilize ES cells in a pluripotent state by maintaining the requisite level of Oct3/4 expression...
  13. pmc Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells
    Shinji Masui
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    BMC Dev Biol 8:45. 2008
    ..However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported...
  14. pmc Maintenance of pluripotency in mouse ES cells without Trp53
    Masaki Shigeta
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan
    Sci Rep 3:2944. 2013
    ..These data indicated that the requirement of Trp53 for maintaining and executing the ES pluripotency is not absolute. ..
  15. pmc DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation
    Masaaki Oda
    Laboratory for Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN, Kobe, Japan
    PLoS Genet 9:e1003574. 2013
    ....
  16. doi request reprint Context-dependent wiring of Sox2 regulatory networks for self-renewal of embryonic and trophoblast stem cells
    Kenjiro Adachi
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 6500047, Japan Electronic address
    Mol Cell 52:380-92. 2013
    ..Our findings provide insights into the functional versatility of transcription factors during embryogenesis, during which they can be recursively utilized in a variable manner within discrete network structures...
  17. pmc Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells
    Yuhki Nakatake
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chu o ku, Kobe 650 0047, Japan
    Mol Cell Biol 26:7772-82. 2006
    ..DNA microarray analysis revealed that a subset of putative Oct3/4 target genes may be regulated in the same manner. Our findings shed light on a novel function of Oct3/4 in ES cells...
  18. ncbi request reprint Bidirectional developmental potential in reprogrammed cells with acquired pluripotency
    Haruko Obokata
    1 Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 2 Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 3 Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 505:676-80. 2014
    ..Taken together, the developmental potential of STAP cells, shown by chimaera formation and in vitro cell conversion, indicates that they represent a unique state of pluripotency. ..
  19. ncbi request reprint Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells
    Kazuya Ogawa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    J Cell Sci 120:55-65. 2007
    ..These findings suggest that endogenously activated autocrine loops of activin-Nodal signaling promote ES cell self-renewal...
  20. doi request reprint The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass
    Noriyuki Nishioka
    Laboratory for Embryonic Induction, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Dev Cell 16:398-410. 2009
    ..Thus, differential signaling between inside and outside cell populations leads to changes in cell fate specification during TE formation...
  21. pmc Kinetics of drug selection systems in mouse embryonic stem cells
    Yuhki Nakatake
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    BMC Biotechnol 13:64. 2013
    ..Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate a transfectant without considering their dose-dependent characters...
  22. pmc An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit
    Shinji Masui
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology Minatojima minamimachi 2 2 3, Kobe, Japan
    Nucleic Acids Res 33:e43. 2005
    ..We believe that use of this system will strongly accelerate molecular biological research using ES cells...
  23. pmc Extra-embryonic endoderm cells derived from ES cells induced by GATA factors acquire the character of XEN cells
    Daisuke Shimosato
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, Minatojima Minamimachi, Chuo Ku, Kobe, Hyogo, Japan
    BMC Dev Biol 7:80. 2007
    ....
  24. doi request reprint Identification and characterization of subpopulations in undifferentiated ES cell culture
    Yayoi Toyooka
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chu o ku, Kobe, Hyogo, Japan
    Development 135:909-18. 2008
    ..These results confirmed that undifferentiated ES cell culture contains subpopulations corresponding to ICM, epiblast and PrE...
  25. doi request reprint Choice of random rather than imprinted X inactivation in female embryonic stem cell-derived extra-embryonic cells
    Kazuhiro Murakami
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology CDB, Kobe, Hyogo 650 0047, Japan
    Development 138:197-202. 2011
    ..Moreover, cloned embryos generated by the transfer of nuclei from the female ES cells showed random Xi in TE, suggesting the complete erasure of all X imprints for imprinted Xi in ICM-derived ES cells...
  26. ncbi request reprint Stimulus-triggered fate conversion of somatic cells into pluripotency
    Haruko Obokata
    1 Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA 2 Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 3 Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nature 505:641-7. 2014
    ..Thus, our findings indicate that epigenetic fate determination of mammalian cells can be markedly converted in a context-dependent manner by strong environmental cues. ..
  27. ncbi request reprint A novel mechanism for regulating clonal propagation of mouse ES cells
    Kazuya Ogawa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chuo Ku, Kobe 650 0047, Japan
    Genes Cells 9:471-7. 2004
    ..Because ES cells themselves produce the same activity, the finding suggests a novel mechanism in which activation of AC restricts clonal propagation of pluripotent stem cells...
  28. ncbi request reprint Synergistic action of Wnt and LIF in maintaining pluripotency of mouse ES cells
    Kazuya Ogawa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Biochem Biophys Res Commun 343:159-66. 2006
    ..These observations indicate that the Wnt signal mediated by the canonical pathway is not sufficient but enhances the effect of LIF to maintain self-renewal of mouse ES cells...
  29. ncbi request reprint Lunatic fringe potentiates Notch signaling in the developing brain
    Tomoaki M Kato
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Mol Cell Neurosci 45:12-25. 2010
    ..In vivo overexpression experiments with Notch ligands suggest that Lfng strongly augments Notch signaling mediated by Delta-like 1 but not Jagged 1...
  30. doi request reprint Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells
    Masatoshi Ohgushi
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 7:225-39. 2010
    ..Thus, the Abr-dependent "Rho-high/Rac-low" state plays a decisive role in initiating the dissociation-induced actomyosin hyperactivation and apoptosis in hESCs...
  31. doi request reprint DNA methylation is dispensable for the growth and survival of the extraembryonic lineages
    Morito Sakaue
    Laboratory for Mammalian Epigenetic Studies, Center for Developmental Biology, RIKEN, 2 2 3, Minatojima Minamimachi, Kobe, Hyogo, 650 0047, Japan
    Curr Biol 20:1452-7. 2010
    ..Our findings indicated that extraembryonic-lineage cells can survive and proliferate in the absence of DNA methyltransferases and that a cell's response to the stress of epigenomic damage is cell type dependent...
  32. ncbi request reprint Clonal expansion of human pluripotent stem cells on gelatin-coated surface
    Hiroyuki Kitajima
    Division of Human Stem Cell Technology, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Biochem Biophys Res Commun 396:933-8. 2010
    ..Furthermore, no chromosomal abnormalities are found even after sequential passage. Therefore this system will dramatically simplify genetic engineering of these human pluripotent stem cells or defining process of their signal pathway...
  33. ncbi request reprint The C-terminal region of Xpc is dispensable for the transcriptional activity of Oct3/4 in mouse embryonic stem cells
    Shunsuke Ito
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7 5 1 Kusunokicho, Chuo Ku, Kobe 650 0017, Japan
    FEBS Lett 588:1128-35. 2014
    ..These data indicated that the C-terminal region of Xpc is dispensable for the transcriptional activity of Oct3/4 in mouse ES cells. ..
  34. ncbi request reprint Transcription factor network in embryonic stem cells: heterogeneity under the stringency
    Yoko Nakai-Futatsugi
    Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Biol Pharm Bull 36:166-70. 2013
    ..Here we focus on recent studies on the heterogeneity of ES cells and discuss their inherent metastability...
  35. pmc The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells
    Satoru Miyagi
    Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, Saitama 350 1241, Japan
    Mol Cell Biol 24:4207-20. 2004
    ..Thus, SRR2 is the first example of an enhancer in which a single regulatory core sequence is involved in multipotent-state-specific expression in two different stem cells, i.e., ES and neural stem cells...
  36. ncbi request reprint Nonylphenol induces the death of neural stem cells due to activation of the caspase cascade and regulation of the cell cycle
    Chiho Kudo
    Department of Pharmacology, Graduate School of Dentistry, Osaka University, Japan
    J Neurochem 88:1416-23. 2004
    ....
  37. ncbi request reprint Diclofenac inhibits proliferation and differentiation of neural stem cells
    Chiho Kudo
    Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Pharmacol 66:289-95. 2003
    ..These results suggest that diclofenac may affect the development of the central nervous system...
  38. ncbi request reprint Genomic organization and promoter analysis of the Dnmt3b gene
    Chisaki Ishida
    Division of Gene Therapy Science, Osaka University School of Medicine, 2 2 Yamada oka, Suita, 565 0870, Osaka, Japan
    Gene 310:151-9. 2003
    ..9 kb upstream of Dnmt3b downregulated this gene specifically in somatic cells but not in ES cells. These findings provide a basis for future detailed studies of the mechanisms controlling Dnmt3b expression...
  39. ncbi request reprint Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing cultures
    Koji Shimozaki
    Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, 860 0811, Japan
    Development 130:2505-12. 2003
    ..In contrast, sustained upregulated Oct3/4 expression enhanced SDIA-mediated neurogenesis of ES cells. Therefore, Oct3/4 appears to promote neuroectoderm formation and subsequent neuronal differentiation from ES cells...
  40. pmc Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development
    Yoshimi Tokuzawa
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara 630 0192, Japan
    Mol Cell Biol 23:2699-708. 2003
    ..Fbx15-null ES cells were normal in morphology, proliferation, and differentiation. These data demonstrate that Fbx15 is a novel target of Oct3/4 but is dispensable for ES cell self-renewal, development, and fertility...
  41. pmc Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells
    Satoshi Tateishi
    Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862 0976, USA
    Mol Cell Biol 23:474-81. 2003
    ..These results indicate that dysfunction of Rad18 greatly increases both the frequency of homologous as well as illegitimate recombination, and that RAD18 contributes to maintenance of genomic stability through postreplication repair...
  42. ncbi request reprint HEX acts as a negative regulator of angiogenesis by modulating the expression of angiogenesis-related gene in endothelial cells in vitro
    Tomowaki Nakagawa
    Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    Arterioscler Thromb Vasc Biol 23:231-7. 2003
    ..The hematopoietically expressed homeobox (HEX) is transiently expressed in endothelial cells (ECs) during vascular formation in embryo. Here, we investigated whether HEX played any role in angiogenesis-related properties of ECs in vitro...
  43. pmc Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity
    Tetsuya S Tanaka
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, 21224 6820, USA
    Genome Res 12:1921-8. 2002
    ..We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage...
  44. pmc Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex
    Mizuho Tomioka
    Division of Developmental Biology, Saitama Medical School Research Center for Genomic Medicine, 1397 1 Yamane Hidaka City, Saitama 350 1241, Japan
    Nucleic Acids Res 30:3202-13. 2002
    ..Co-transfection analyses confirm that both complexes are able to stimulate transcription through the SRR2 element...
  45. pmc Differentiation of embryonic stem cells is induced by GATA factors
    Junji Fujikura
    Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Genes Dev 16:784-9. 2002
    ..We believe that this is the first report of a physiological differentiation event induced by the ectopic expression of a transcription factor in ES cells...
  46. pmc Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells
    Hitoshi Niwa
    Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita C, Osaka 565 0871, Japan
    Mol Cell Biol 22:1526-36. 2002
    ..Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation...
  47. pmc Prox1 induces lymphatic endothelial differentiation via integrin alpha9 and other signaling cascades
    Koichi Mishima
    Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Mol Biol Cell 18:1421-9. 2007
    ....
  48. ncbi request reprint Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells
    Sayaka Okumura-Nakanishi
    Laboratory of Molecular and Cellular Assembly, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatuda, Midori ku, Yokohama 226 8501, Japan
    J Biol Chem 280:5307-17. 2005
    ..This autoregulatory circuit of the Sox2.Oct-3/4 complex may contribute to maintaining robustly the precise expression level of Oct-3/4 in primitive cells...
  49. ncbi request reprint Regulation of mesodermal differentiation of mouse embryonic stem cells by basement membranes
    Hironobu Fujiwara
    Institute for Protein Research, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 282:29701-11. 2007
    ..Taken together, these results indicate that the BM prevents the EMT and precocious differentiation of primitive ectoderm toward mesoderm in EBs, implying that BMs are important for the control of mammalian gastrulation...
  50. ncbi request reprint Inhibition of DNA binding of Sox2 by the SUMO conjugation
    Shu Tsuruzoe
    Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Biochem Biophys Res Commun 351:920-6. 2006
    ..Further, SUMO-1-conjugated Sox2 at the lysine 247 or at the carboxyl terminus reduced the binding to the Fgf4 enhancer. These indicate that Sox2 sumoylation negatively regulates its transcriptional role through impairing the DNA binding...
  51. ncbi request reprint [Transcriptional network maintaining pluripotency of embryonic stem cells]
    Shinji Masui
    Tanpakushitsu Kakusan Koso 53:164-8. 2008
  52. doi request reprint Requirement of Oct3/4 function for germ cell specification
    Daiji Okamura
    Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Sendai 980 8575, Japan
    Dev Biol 317:576-84. 2008
    ..In the presence of Dox, Oct3/4 protein was absent in the nuclei of the ES-derived cells, which failed to form PGCs. In contrast, the ES-derived cells could be specified to PGCs after activation of Oct3/4 function in the presence of Dex...
  53. ncbi request reprint Nuclear and chromatin reorganization in the MHC-Oct3/4 locus at developmental phases of embryonic stem cell differentiation
    Takahiro Aoto
    Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, The 21st Century COE, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Dev Biol 298:354-67. 2006
    ..These findings provide insights into the molecular basis of global nuclear reorganization and euchromatic gene silencing in differentiation through the spatiotemporal order of epigenetic controls...
  54. doi request reprint Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells
    Chuanhai Sun
    Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, Ishikawa 920 8640, Japan
    Biochem Biophys Res Commun 372:91-6. 2008
    ..Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells...
  55. pmc Oct-3/4 maintains the proliferative embryonic stem cell state via specific binding to a variant octamer sequence in the regulatory region of the UTF1 locus
    Masazumi Nishimoto
    Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, 1397 1 Yamane, Hidaka, Saitama 350 1241, Japan
    Mol Cell Biol 25:5084-94. 2005
    ..Moreover, UTF1 was also observed to have an effect on teratoma formation. These results suggest a molecular pathway by which Oct-3/4 induces rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene...
  56. doi request reprint Consequence of the loss of Sox2 in the developing brain of the mouse
    Satoru Miyagi
    Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397 1 Yamane, Hidaka, Saitama 350 1241, Japan
    FEBS Lett 582:2811-5. 2008
    ..We found that expression level of Sox3 is elevated in Sox2 null developing brain, probably mitigating the effects of loss of Sox2...
  57. ncbi request reprint Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells
    Tadayuki Akagi
    Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 8640, Japan
    Biochem Biophys Res Commun 331:23-30. 2005
    ..These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal...
  58. ncbi request reprint Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality
    Masato Sasaki
    Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryomachi, Aoba ku, Sendai 980 8575, Japan
    Mech Dev 124:489-99. 2007
    ..The possible mechanisms for the early pre-implantation lethality of PP2Cbeta(Delta/Delta) mice are discussed...
  59. ncbi request reprint Nitration of PPARgamma inhibits ligand-dependent translocation into the nucleus in a macrophage-like cell line, RAW 264
    Atsuhito Shibuya
    Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1 8 Yamadaoka, Suita, 565 0871, Osaka, Japan
    FEBS Lett 525:43-7. 2002
    ....
  60. ncbi request reprint Essential role for ERK2 mitogen-activated protein kinase in placental development
    Naoya Hatano
    Department of Pathology and Pathophysiology, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Japan
    Genes Cells 8:847-56. 2003
    ..Extracellular signal-regulated kinase 2 (ERK2) has been implicated in cell proliferation, differentiation, and survival. However, its role in vivo remains to be determined...
  61. pmc Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis
    Seiji Takashima
    Department of Internal Medicine and Therapeutics, Department of Nutrition and Physiological Chemistry, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 99:3657-62. 2002
    ..Their abnormal vascular phenotype resembled those of VEGF and VEGFR-2 knockouts. These results suggest that NRPs are early genes in embryonic vessel development and that both NP1 and NP2 are required...
  62. ncbi request reprint Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells
    Megumi Hirose
    Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565 0871, Japan
    Regul Pept 126:109-13. 2005
    ..These results suggest that this in vitro neuronal culture system will be a useful model for future studies on the functional role of the PACAPergic system during different stages of neuronal development...
  63. ncbi request reprint Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-3
    Hiroki Umehara
    Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamada oka, Suita, Osaka, Japan
    Stem Cells 25:2705-11. 2007
    ..In contrast, Akt signaling activation enhanced the growth of ICM but did not increase the efficiency of ES cell derivation. Our study establishes an efficient means for ES cell derivation by pharmacological inhibition of GSK-3...