Atsushi Miyawaki

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. pmc mKikGR, a monomeric photoswitchable fluorescent protein
    Satoshi Habuchi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 3:e3944. 2008
  2. ncbi request reprint Visualization of the spatial and temporal dynamics of intracellular signaling
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Dev Cell 4:295-305. 2003
  3. ncbi request reprint Mechanisms of protein fluorophore formation and engineering
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Curr Opin Chem Biol 7:557-62. 2003
  4. ncbi request reprint Green fluorescent protein-like proteins in reef Anthozoa animals
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, Saitama, Japan
    Cell Struct Funct 27:343-7. 2002
  5. ncbi request reprint Lighting up cells: labelling proteins with fluorophores
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Nat Cell Biol . 2003
  6. ncbi request reprint [Fluorescent proteins found in cnidarian animals]
    Atsushi Miyawaki
    Tanpakushitsu Kakusan Koso 48:1568-72. 2003
  7. ncbi request reprint A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling
    Satoshi Karasawa
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    J Biol Chem 278:34167-71. 2003
  8. ncbi request reprint Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Science 306:1370-3. 2004
  9. ncbi request reprint PKC signaling mediates global enhancement of excitatory synaptogenesis in neurons triggered by local contact with astrocytes
    Hiroshi Hama
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Neuron 41:405-15. 2004
  10. ncbi request reprint Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy
    Hiroshi Hashimoto
    RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Appl Opt 49:3323-9. 2010

Collaborators

Detail Information

Publications95

  1. pmc mKikGR, a monomeric photoswitchable fluorescent protein
    Satoshi Habuchi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 3:e3944. 2008
    ..Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging...
  2. ncbi request reprint Visualization of the spatial and temporal dynamics of intracellular signaling
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Dev Cell 4:295-305. 2003
    ....
  3. ncbi request reprint Mechanisms of protein fluorophore formation and engineering
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Curr Opin Chem Biol 7:557-62. 2003
    ..Further molecular characterization of the structure and maturation of these proteins is in progress, aimed at providing information for rational design of variants with desired fluorescence properties...
  4. ncbi request reprint Green fluorescent protein-like proteins in reef Anthozoa animals
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, Saitama, Japan
    Cell Struct Funct 27:343-7. 2002
    ..The ubiquity of these molecular tools makes it important to appreciate the interplay between sunlight and GFP-like proteins of Anthozoan animals, and to consider the optimal use of these unique proteins in biological studies...
  5. ncbi request reprint Lighting up cells: labelling proteins with fluorophores
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Nat Cell Biol . 2003
    ..In addition, new techniques have made it possible to label proteins with small organic fluorophores and semiconductor nanocrystals...
  6. ncbi request reprint [Fluorescent proteins found in cnidarian animals]
    Atsushi Miyawaki
    Tanpakushitsu Kakusan Koso 48:1568-72. 2003
  7. ncbi request reprint A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling
    Satoshi Karasawa
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    J Biol Chem 278:34167-71. 2003
    ..Thus, monomeric AG is a useful monomeric green-emitting fluorescent protein comparable to EGFP...
  8. ncbi request reprint Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Science 306:1370-3. 2004
    ..Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules...
  9. ncbi request reprint PKC signaling mediates global enhancement of excitatory synaptogenesis in neurons triggered by local contact with astrocytes
    Hiroshi Hama
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Neuron 41:405-15. 2004
    ..Thus, propagation of PKC signaling represents an underlying mechanism for global neuronal maturation following local astrocyte adhesion...
  10. ncbi request reprint Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy
    Hiroshi Hashimoto
    RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Appl Opt 49:3323-9. 2010
    ..By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution...
  11. ncbi request reprint Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer
    Satoshi Shimozono
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Biochemistry 45:6267-71. 2006
    ..5 were irradiated at 514.5 nm, a 10-fold increase in the 475 nm fluorescence intensity was observed. These features make Cy11.5 useful as an optical highlighter and a new-colored fluorescent protein for multicolor imaging...
  12. doi request reprint Tracing the silhouette of individual cells in S/G2/M phases with fluorescence
    Asako Sakaue-Sawano
    Life Function and Dynamics, ERATO, JST, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Chem Biol 15:1243-8. 2008
    ..We also show that nuclear localization of the ubiquitin ligase for Geminin is essential for full performance of the markers...
  13. doi request reprint Software for precise tracking of cell proliferation
    Hiroshi Kurokawa
    Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Biochem Biophys Res Commun 417:1080-5. 2012
    ..By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time...
  14. ncbi request reprint Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse
    Keisuke Isobe
    Keisuke Isobe Laser Technology Laboratory, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Opt Express 17:11259-66. 2009
    ..Vibrational spectral imaging of chemical and biological samples is demonstrated using the two types of microscopy...
  15. pmc Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer
    Satoshi Karasawa
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Biochem J 381:307-12. 2004
    ....
  16. pmc A practical device for pinpoint delivery of molecules into multiple neurons in culture
    Chikako Hara
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Brain Cell Biol 35:229-37. 2006
    ..In addition, the nano-sized needle tip enables gentle molecular delivery, minimizing cell damage. This method permits DNA transfection into specific hippocampal neurons without disturbing neuronal circuitry established in culture...
  17. doi request reprint Fluorescence imaging using a fluorescent protein with a large Stokes shift
    Takako Kogure
    Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Methods 45:223-6. 2008
    ..Keima can be used in conjunction with existing fluorescent proteins in which the Stokes shift is much smaller, with the idea that while two fluorescent proteins are excited by a single laser each will fluoresce a different color...
  18. doi request reprint Molecular basis of photochromism of a fluorescent protein revealed by direct 13C detection under laser illumination
    Hideaki Mizuno
    Cell Function and Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    J Biomol NMR 48:237-46. 2010
    ....
  19. doi request reprint Transgenic zebrafish for ratiometric imaging of cytosolic and mitochondrial Ca2+ response in teleost embryo
    Hideaki Mizuno
    Cell Function and Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Cell Calcium 54:236-45. 2013
    ..This is in contrast with the transient rise in the cytosol Ca2+ that directly evokes the muscle contraction. These transgenic zebrafish lines are expected to serve as useful tools further Ca2+ imaging in vivo...
  20. doi request reprint The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins
    Hidekazu Tsutsui
    Brain Science Institute, RIKEN, Hirosawa, Wako City, Saitama, Japan
    Chem Biol 16:1140-7. 2009
    ..This structural rearrangement provides evidence that the beta-elimination reaction governing the green-to-red photoconversion of KikGR follows an E1 (elimination, unimolecular) mechanism...
  21. doi request reprint Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain
    Hiroshi Hama
    Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Nat Neurosci 14:1481-8. 2011
    ..Our findings suggest that the Scale method will be useful for light microscopy-based connectomics of cellular networks in brain and other tissues...
  22. ncbi request reprint Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses
    Keisuke Isobe
    Laser Technology Laboratory, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Opt Express 17:13737-46. 2009
    ..We also demonstrate yielded equal excitation rates in the simultaneous excitation. By the selective excitation of a donor fluorescent protein, fluorescence resonance energy transfer imaging is also achieved...
  23. pmc Diffusion of large molecules into assembling nuclei revealed using an optical highlighting technique
    Satoshi Shimozono
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, Institute of Physical and Chemical Research, Saitama, Japan
    Biophys J 97:1288-94. 2009
    ..Because KikGR contains no known nuclear-localization or chromosome-binding sequences, our results indicate the diffusion barrier is less restrictive during nuclear reassembly...
  24. ncbi request reprint A high-throughput method for development of FRET-based indicators for proteolysis
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 319:72-7. 2004
    ..This new high-throughput method will be applicable to development and improvement of FRET-based indicators for proteolysis...
  25. doi request reprint Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging
    Takashi Fukano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Brain Cell Biol 36:43-52. 2008
    ..This system increases the rate at which ratio measurements are made to 1 kHz, and provides ratio images at 10-100 Hz depending on the signal intensity...
  26. ncbi request reprint GFP-like proteins stably accumulate in lysosomes
    Hiroyuki Katayama
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN
    Cell Struct Funct 33:1-12. 2008
    ....
  27. pmc Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects
    Kiwamu Takemoto
    Laboratory for Cell Recovery Mechanisms, Advanced Technology Development Center, RIKEN Brain Science Institute, Wako, Saitama 351 0198, Japan
    J Cell Biol 160:235-43. 2003
    ....
  28. pmc An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 99:12651-6. 2002
    ..Illumination of a focused UV pulse onto the soma of a Kaede-expressing neuron resulted in filling of all processes with red fluorescence, allowing visualization of contact sites between the red and green neurons of interest...
  29. pmc Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication
    Asako Sakaue-Sawano
    Life Function and Dynamics, ERATO, JST, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    BMC Cell Biol 12:2. 2011
    ..Cytometry analysis only quantifies dye-incorporation to examine DNA content and does not reflect the biological complexity of the cell cycle in drug discovery screens...
  30. pmc Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system
    Michio Tomura
    Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN, Yokohama City, Kanagawa, Japan Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako City, Saitama, Japan Center for Innovation in Immunoregulative Technology and Therapeutics, Kyoto University Graduate School of Medicine, Kyoto City, Japan
    PLoS ONE 8:e73801. 2013
    ..We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses. ..
  31. pmc Analysis of aquaporin-mediated diffusional water permeability by coherent anti-stokes Raman scattering microscopy
    Keiji Ibata
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    Biophys J 101:2277-83. 2011
    ..These results suggest the possibility of using CARS imaging to investigate the hydrodynamics of single mammalian cells as well as the regulation of AQPs...
  32. ncbi request reprint Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein
    Satoshi Shimozono
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, Wako City, Saitama, Japan
    Sci STKE 2002:pl4. 2002
    ..We demonstrate that this new confocal imaging system expands the range of potential applications of ratiometric-pericam and other dual-excitation ratiometric indicators...
  33. pmc Light-dependent regulation of structural flexibility in a photochromic fluorescent protein
    Hideaki Mizuno
    Cell Function and Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 105:9227-32. 2008
    ..These interactions are disrupted by strong illumination with blue light, and the chromophore, together with a part of the beta-barrel, becomes flexible, leading to a nonradiative decay process...
  34. doi request reprint Higher resolution in localization microscopy by slower switching of a photochromic protein
    Hideaki Mizuno
    Cell Function and Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Photochem Photobiol Sci 9:239-48. 2010
    ..Similarly we find that higher-resolution PALM images can be acquired with slower-switching proteins due to their higher number of emitted photons per switching cycle...
  35. ncbi request reprint Engineering FRET constructs using CFP and YFP
    Satoshi Shimozono
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Methods Cell Biol 85:381-93. 2008
    ..This approach has become even more powerful due to the construction of a new pair of fluorescent proteins for FRET: CyPet and YPet...
  36. ncbi request reprint Lateral propagation of EGF signaling after local stimulation is dependent on receptor density
    Asako Sawano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Dev Cell 3:245-57. 2002
    ..We thus present evidence that ligand-independent propagation of EGF signaling occurs only when the receptor density on the plasma membrane is high, such as in carcinoma cells...
  37. ncbi request reprint Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights
    Takashi Fukano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako Shi, Saitama ken 351 0198, Japan
    Biochem Biophys Res Commun 317:77-83. 2004
    ..This methodology allows us to perform simultaneous measurements of any dual-excitation ratiometric dye, and we demonstrate its validity by monitoring [Ca2+] changes in rat cardiac muscle cells loaded with Fura Red...
  38. ncbi request reprint Competition between energy and proton transfer in ultrafast excited-state dynamics of an oligomeric fluorescent protein red Kaede
    Haruko Hosoi
    Molecular Spectroscopy Laboratory, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama 351 0198, Japan
    J Phys Chem B 110:22853-60. 2006
    ..The ESPT rate in red Kaede was significantly slower than the rate in Aequorea GFP, which highly likely arises from the different hydrogen bond network around the chromophore...
  39. doi request reprint Visualization of an endogenous retinoic acid gradient across embryonic development
    Satoshi Shimozono
    Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Nature 496:363-6. 2013
    ....
  40. pmc Illuminating cell-cycle progression in the developing zebrafish embryo
    Mayu Sugiyama
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 106:20812-7. 2009
    ..Our study demonstrates the effectiveness of using the Cul4(Ddb1)-mediated Cdt1 degradation pathway common to all metazoans for the development of a G(1) marker that works in the nonmammalian animal model...
  41. pmc Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells
    Toru Matsu-Ura
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, Saitama 351 0198, Japan
    J Cell Biol 173:755-65. 2006
    ....
  42. ncbi request reprint Similar diffusibility of membrane proteins across the axon-soma and dendrite-soma boundaries revealed by a novel FRAP technique
    Takashi Fukano
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    J Struct Biol 147:12-8. 2004
    ..These results indicate that the secondary diffusion barrier to exogenously overexpressed membrane proteins is not specific to the axon-soma boundary...
  43. pmc Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:10554-9. 2004
    ..Our study provides an important guide for the development and improvement of indicators using GFP-based FRET...
  44. ncbi request reprint Development of genetically encoded fluorescent indicators for calcium
    Atsushi Miyawaki
    Laboratory for Cell Function and Dynamics, Brain Science Institute, Institute of Physical and Chemical Research, Wako City, Saitama, Japan
    Methods Enzymol 360:202-25. 2003
  45. ncbi request reprint A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy
    Takako Kogure
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 24:577-81. 2006
    ..In addition, Keima and a spectral variant that emits maximally at 570 nm might facilitate simultaneous multicolor imaging with single-wavelength excitation...
  46. ncbi request reprint Engineering fluorescent proteins
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198 Japan
    Adv Biochem Eng Biotechnol 95:1-15. 2005
    ..Further molecular characterization of the structure and maturation of these proteins is in progress, aimed at providing information for rational design of variants with desired fluorescence properties...
  47. doi request reprint Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
    Asako Sakaue-Sawano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Cell 132:487-98. 2008
    ..These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events...
  48. ncbi request reprint Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells
    Tetsuya Kitaguchi
    Life Function and Dynamics, ERATO, JST Japan Science and Technology Agency, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 450:365-73. 2013
    ..Taken together, the findings of the present study demonstrate that ADCY1 plays an important role in the control of pancreatic β-cell cAMP homoeostasis and insulin secretion...
  49. pmc Semi-rational engineering of a coral fluorescent protein into an efficient highlighter
    Hidekazu Tsutsui
    Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    EMBO Rep 6:233-8. 2005
    ..In addition, KikGR was successfully photoconverted using two-photon excitation microscopy at 760 nm, ensuring optical cell labelling with better spatial discrimination in thick and highly scattering tissues...
  50. doi request reprint Hidden electronic excited state of enhanced green fluorescent protein
    Haruko Hosoi
    Molecular Spectroscopy Laboratory, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Phys Chem B 112:2761-3. 2008
    ....
  51. doi request reprint Improving membrane voltage measurements using FRET with new fluorescent proteins
    Hidekazu Tsutsui
    Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Saitama 351 0198, Japan
    Nat Methods 5:683-5. 2008
    ..Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials...
  52. doi request reprint A bilirubin-inducible fluorescent protein from eel muscle
    Akiko Kumagai
    Cell Function Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Cell 153:1602-11. 2013
    ..UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering. ..
  53. ncbi request reprint Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein
    Hideaki Mizuno
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, The Institute of Physical and Chemical Science RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Mol Cell 12:1051-8. 2003
    ..The present study not only reveals diversity in the chemical structure of fluorescent proteins but also adds a new dimension to posttranslational modification mechanisms...
  54. ncbi request reprint Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass
    Yasutaka Hanada
    RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biomed Microdevices 10:403-10. 2008
    ..Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells...
  55. doi request reprint 3D microfluidic chips with integrated functional microelements fabricated by a femtosecond laser for studying the gliding mechanism of cyanobacteria
    Yasutaka Hanada
    RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama, Japan
    Lab Chip 11:2109-15. 2011
    ..Using this apparatus, we found that CO(2) secreted from the seedling root attracts Phormidium in the presence of light, and determined the light intensity and specific wavelength necessary for gliding...
  56. ncbi request reprint Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses
    Hiroyuki Kawano
    Laser Technology Laboratory, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 311:592-6. 2003
    ..Our method will provide a powerful solution to various problems confronting researchers, such as the photobleaching of dyes in two-photon excitation microscopy...
  57. doi request reprint Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions
    Michio Tomura
    Research Center for Allergy and Immunology, RIKEN, Yokohama Institute, Tsurumi, Yokohama City, Kanagawa, Japan
    Int Immunol 21:1145-50. 2009
    ..Taken together, time-lapse observation of cellular interaction with functional probe, SCAT3.1 provides new kinetic information and demonstrates that co-stimulatory molecules regulate the kinetics of CTL-target cell interaction...
  58. ncbi request reprint Differential Ras activation between caveolae/raft and non-raft microdomains
    Takashi Fukano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN
    Cell Struct Funct 32:9-15. 2007
    ..We have used this system to demonstrate that, upon stimulation with growth factors, the two indicators are activated in spatially and temporally unique patterns...
  59. ncbi request reprint Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters
    Takashi Fukano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biochem Biophys Res Commun 340:250-5. 2006
    ..We demonstrate the effectiveness of this system by monitoring changes in [Ca2+] in cardiac muscle cells loaded with Fura-2...
  60. pmc A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis
    Mitsuhiro Abe
    Lipid Biology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan
    Mol Cell Biol 32:1396-407. 2012
    ..These results suggest that the formation of SM-rich domains is required for the accumulation of PIP(2) to the cleavage furrow, which is a prerequisite for the proper translocation of RhoA and the progression of cytokinesis...
  61. doi request reprint Proteins on the move: insights gained from fluorescent protein technologies
    Atsushi Miyawaki
    Life Function and Dynamics, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Saitama, Japan
    Nat Rev Mol Cell Biol 12:656-68. 2011
    ..These technologies have greatly advanced our understanding of protein dynamics, including protein movement and protein interactions...
  62. doi request reprint Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer
    Atsushi Miyawaki
    Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, Wako City, Saitama 351 0198, Japan
    Annu Rev Biochem 80:357-73. 2011
    ..The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed...
  63. pmc Multicolor imaging of Ca(2+) and protein kinase C signals using novel epifluorescence microscopy
    Asako Sawano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Biophys J 82:1076-85. 2002
    ..By observing fluorescence resonance energy transfer, we also obtained direct evidence that Ca(2+)-CaM binds MARCKS to drag it away from the membrane in circumstances when Ca(2+)-mobilization predominates over PKC activation...
  64. ncbi request reprint Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone
    Takuro Tojima
    Laboratory for Neuronal Growth Mechanisms, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nat Neurosci 10:58-66. 2007
    ..These results strongly suggest that growth cone attraction and repulsion are driven by distinct mechanisms, rather than using the same molecular machinery with opposing polarities...
  65. pmc Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants
    Ryoko Ando
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, The Institute of Physical and Chemical Research, Hirosawa, Wako City, Saitama 351 0198, Japan
    Biophys J 92:L97-9. 2007
    ..Simultaneous 488- and 405-nm irradiation, however, results in a rapid oscillation between the two states, thereby keeping the emissive state population large enough to produce sufficiently bright fluorescence signals...
  66. ncbi request reprint Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples
    Takashi Fukano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Institute of Brain Science, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Appl Opt 42:4119-24. 2003
    ..We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications...
  67. ncbi request reprint Fluorescence imaging of physiological activity in complex systems using GFP-based probes
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Curr Opin Neurobiol 13:591-6. 2003
    ..Optical imaging using genetically encoded probes has enabled us to decipher spatio-temporal information coded in complex tissues...
  68. pmc Slow Ca2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping
    Satoshi Shimozono
    Laboratory for Cell Function Dynamics, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    EMBO Rep 5:521-6. 2004
    ..The excitation-calcium coupling may be uniquely modulated in this region at the level of calcium channels on the plasma membrane...
  69. pmc Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics
    An a Kazuno
    Laboratory for Molecular Dynamics of Mental Disorders, Brain Science Institute, RIKEN, Saitama, Japan
    PLoS Genet 2:e128. 2006
    ..Our findings suggest that these mtDNA polymorphisms may play a role in the pathophysiology of these complex diseases by affecting mitochondrial matrix pH and intracellular calcium dynamics...
  70. doi request reprint A poly(dimethylsiloxane)-based device enabling time-lapse imaging with high spatial resolution
    Masahiko Hirano
    Japan Science and Technology Agency, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 392:307-10. 2010
    ..A time-lapse imaging experiment using HeLa cells stably expressing fluorescent cell-cycle indicators showed that the cells in the chamber proliferated with normal cell-cycle period over 2 days...
  71. pmc Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice
    Michio Tomura
    Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama City, Kanagawa 230 0045, Japan
    Proc Natl Acad Sci U S A 105:10871-6. 2008
    ..Thus, the Kaede transgenic mouse system would be an ideal tool to monitor precise cellular movement in vivo at different stages of immune response to pathogens as well as in autoimmune diseases...
  72. ncbi request reprint A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  73. ncbi request reprint Memorizing spatiotemporal patterns
    Atsushi Miyawaki
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Nat Chem Biol 3:598-601. 2007
  74. ncbi request reprint Electrical stimulation modulates fate determination of differentiating embryonic stem cells
    Masahisa Yamada
    Laboratory for Cell Culture Development, Yamada Research Unit, Molecular Neuropathology Group, Institute of Physical and Chemical Research, Saitama 351 0198, Japan
    Stem Cells 25:562-70. 2007
    ..This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts...
  75. ncbi request reprint Innovations in the imaging of brain functions using fluorescent proteins
    Atsushi Miyawaki
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Neuron 48:189-99. 2005
    ..In this primer, I will describe how this approach has met neuroscientists' demands and desires...
  76. ncbi request reprint Nanorheology measurement on single circularly permuted green fluorescent protein molecule
    Tong Wang
    Local Spatio Temporal Functions Laboratory, Frontier Research System, RIKEN, Wako, Saitama 351 0198, Japan
    Colloids Surf B Biointerfaces 40:183-7. 2005
    ..In this measurement, several cycles of sinusoidal movement were applied to the sample during the stretching process. We found the protein molecule showed in-phase response to the sinusoidal input in most case of measurements...
  77. ncbi request reprint Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon
    Yusuke Tsuruwaka
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Zebrafish 4:253-60. 2007
    ..12 was used. These results suggested that YC2.12 is useful for noninvasive and real-time detection of dynamic Ca2+ change throughout gastrulation...
  78. doi request reprint Two-photon dual-color imaging using fluorescent proteins
    Hiroyuki Kawano
    Nat Methods 5:373-4. 2008
  79. ncbi request reprint Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons
    Masayuki Ikeda
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, 565 0874, Osaka, Japan
    Neuron 38:253-63. 2003
    ..Tetrodotoxin blocked MUA, but not Ca(2+) rhythms, while ryanodine damped both Ca(2+) and MUA rhythms. These results demonstrate cytosolic Ca(2+) rhythms regulated by the release of Ca(2+) from ryanodine-sensitive stores in SCN neurons...
  80. pmc Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
    Mazahir T Hasan
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 2:e163. 2004
    ....
  81. ncbi request reprint Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity
    Agata Rekas
    Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 277:50573-8. 2002
    ..F64L induced large conformational changes in the molecule, leading to the removal of halide sensitivity by preventing ion access to the binding site...
  82. ncbi request reprint Fluorescent proteins in a new light
    Atsushi Miyawaki
    Nat Biotechnol 22:1374-6. 2004
  83. ncbi request reprint Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins
    Xuena Lin
    Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 280:12820-32. 2005
    ....
  84. ncbi request reprint Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons
    Keizo Takao
    RIKEN Massachusetts Institute of Technology MIT Neuroscience Research Center, The Picower Center for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, Massachusetts 02139, USA
    J Neurosci 25:3107-12. 2005
    ..In combination with two-photon microscopy, we demonstrate that Camuialpha can be used to observe temporal and spatial regulation of CaMKII activity in living neurons...
  85. ncbi request reprint Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede
    Ikuko Hayashi
    Division of Signaling Biology, Ontario Cancer Institute and Department of Medical Biophysics, Toronto, Ontario, Canada
    J Mol Biol 372:918-26. 2007
    ..We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule...
  86. doi request reprint Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange
    Akihiro Kikuchi
    Biometal Science Laboratory, RIKEN SPring 8 Center, 1 1 1, Kouto, Sayo, Hyogo 679 5148, Japan
    Biochemistry 47:11573-80. 2008
    ..These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO...
  87. ncbi request reprint Calcium indicators based on calmodulin-fluorescent protein fusions
    Kevin Truong
    Institute of Biomaterials and Biomedical Engineering, Department of Electrical and Computer Engineering, University of Toronto, Ontario, Canada
    Methods Mol Biol 352:71-82. 2007
    ..This chapter describes the methods involved in cloning chameleons, characterizing their biochemical and biophysical properties, and imaging them in single cells using a digital fluorescence microscope...
  88. ncbi request reprint Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus
    Mitchell C Y Chan
    Division of Signaling Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 281:37813-9. 2006
    ..We conclude that the dynamics and structure of the chromophore are both essential for the optical appearance of these color proteins...
  89. pmc Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Proc Natl Acad Sci U S A 102:9511-6. 2005
    ..The fast response of Dronpa to light holds great promise for following fast diffusion or transport of signaling molecules in live cells...
  90. doi request reprint Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein
    Tomoki Matsuda
    Laboratory for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University, Kita 12 Nishi 6 Kita ku, Sapporo, Hokkaido, 060 0812, Japan
    Nat Methods 5:339-45. 2008
    ..1 microm2/s up to approximately 100 microm2/s, and found significant changes in free protein movement during cell-cycle progression...
  91. ncbi request reprint The C. elegans thermosensory neuron AFD responds to warming
    Koutarou D Kimura
    Group of Molecular Neurobiology, Department of Molecular Biology, Graduate School of Science, Nagoya University, Japan
    Curr Biol 14:1291-5. 2004
    ..We suggest that C. elegans provides an ideal model to genetically and physiologically reveal the molecular mechanism for sensation and memory of temperature information...
  92. ncbi request reprint [Chemistry in studies of fluorescent proteins]
    Atsushi Miyawaki
    Tanpakushitsu Kakusan Koso 52:1558-62. 2007
  93. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
    ....
  94. ncbi request reprint Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
    ..We experimentally demonstrate the possibility of obtaining increased resolution by making use of the efficient and thermally stable Dronpa photoswitching, using equipment that is commonly available...
  95. ncbi request reprint Ultrafast excited-state dynamics of the photoswitchable protein Dronpa
    Eduard Fron
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:4870-1. 2007