Affiliation: RIKEN Brain Science Institute
- An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unitShinji Masui
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology Minatojima minamimachi 2 2 3, Kobe, Japan
Nucleic Acids Res 33:e43. 2005..We believe that use of this system will strongly accelerate molecular biological research using ES cells...
- Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cellsShinji Masui
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chu o ku, Kobe, Hyogo 650 0047, Japan
Nat Cell Biol 9:625-35. 2007..These results indicate that the essential function of Sox2 is to stabilize ES cells in a pluripotent state by maintaining the requisite level of Oct3/4 expression...
- Rex1/Zfp42 is dispensable for pluripotency in mouse ES cellsShinji Masui
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
BMC Dev Biol 8:45. 2008..However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported...
- Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cellsYuhki Nakatake
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chu o ku, Kobe 650 0047, Japan
Mol Cell Biol 26:7772-82. 2006..DNA microarray analysis revealed that a subset of putative Oct3/4 target genes may be regulated in the same manner. Our findings shed light on a novel function of Oct3/4 in ES cells...
- Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cellsHitoshi Niwa
Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita C, Osaka 565 0871, Japan
Mol Cell Biol 22:1526-36. 2002..Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation...
- The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cellsSatoru Miyagi
Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, Saitama 350 1241, Japan
Mol Cell Biol 24:4207-20. 2004..Thus, SRR2 is the first example of an enhancer in which a single regulatory core sequence is involved in multipotent-state-specific expression in two different stem cells, i.e., ES and neural stem cells...
- [Transcriptional network maintaining pluripotency of embryonic stem cells]Shinji Masui
Tanpakushitsu Kakusan Koso 53:164-8. 2008
- Differentiation of embryonic stem cells is induced by GATA factorsJunji Fujikura
Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
Genes Dev 16:784-9. 2002..We believe that this is the first report of a physiological differentiation event induced by the ectopic expression of a transcription factor in ES cells...
- Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation dataAlexei A Sharov
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA
BMC Genomics 9:269. 2008..However, many responding genes with binding sites may not be direct targets because response may be mediated by other genes and ChIP-binding site may not be functional in terms of transcription regulation...
- Consequence of the loss of Sox2 in the developing brain of the mouseSatoru Miyagi
Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397 1 Yamane, Hidaka, Saitama 350 1241, Japan
FEBS Lett 582:2811-5. 2008..We found that expression level of Sox3 is elevated in Sox2 null developing brain, probably mitigating the effects of loss of Sox2...