Shinobu Kitazume

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. pmc Soluble amyloid precursor protein 770 is released from inflamed endothelial cells and activated platelets: a novel biomarker for acute coronary syndrome
    Shinobu Kitazume
    Disease Glycomics Team, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 287:40817-25. 2012
  2. doi request reprint Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo
    Shinobu Kitazume
    Glyco chain Functions Laboratory, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycobiology 19:479-87. 2009
  3. ncbi request reprint Involvement of proteases in glycosyltransferase secretion: Alzheimer's beta-secretase-dependent cleavage and a following processing by an aminopeptidase
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Frontier Research System, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycoconj J 21:25-9. 2004
  4. ncbi request reprint Beta-galactoside alpha2,6-sialyltransferase I cleavage by BACE1 enhances the sialylation of soluble glycoproteins. A novel regulatory mechanism for alpha2,6-sialylation
    Ichiro Sugimoto
    Glyco chain Functions Laboratory, Institute of Physical and Chemical Research, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 282:34896-903. 2007
  5. ncbi request reprint In vivo cleavage of alpha2,6-sialyltransferase by Alzheimer beta-secretase
    Shinobu Kitazume
    Glyco chain Functions Laboratory, The Institute of Physical and Chemical Research, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 280:8589-95. 2005
  6. ncbi request reprint Characterization of alpha 2,6-sialyltransferase cleavage by Alzheimer's beta -secretase (BACE1)
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Supra biomolecular System Group, Frontier Research System, Brain Science Institute, Institute of Physical and Chemical Research, RIKEN, Saitama 51 0198, Japan
    J Biol Chem 278:14865-71. 2003
  7. pmc Alpha2,6-sialic acid on platelet endothelial cell adhesion molecule (PECAM) regulates its homophilic interactions and downstream antiapoptotic signaling
    Shinobu Kitazume
    Disease Glycomics Team, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 285:6515-21. 2010
  8. ncbi request reprint Interleukin-1 beta up-regulates TACE to enhance alpha-cleavage of APP in neurons: resulting decrease in Abeta production
    Yuriko Tachida
    Glyco chain Functions Laboratory, Supra biomolecular System Group, Frontier Research System, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    J Neurochem 104:1387-93. 2008
  9. ncbi request reprint Sialylation enhances the secretion of neurotoxic amyloid-beta peptides
    Kazuhiro Nakagawa
    Glycochain Functions Laboratory, Suprabiomolecular System Group, Frontier Research System, RIKEN, Wako Shi, Saitama, Japan
    J Neurochem 96:924-33. 2006
  10. pmc Brain endothelial cells produce amyloid {beta} from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form
    Shinobu Kitazume
    Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 285:40097-103. 2010

Collaborators

Detail Information

Publications25

  1. pmc Soluble amyloid precursor protein 770 is released from inflamed endothelial cells and activated platelets: a novel biomarker for acute coronary syndrome
    Shinobu Kitazume
    Disease Glycomics Team, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 287:40817-25. 2012
    ..Separate monitoring of the cleavage products of different amyloid β precursor protein (APP) variants may provide useful information...
  2. doi request reprint Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo
    Shinobu Kitazume
    Glyco chain Functions Laboratory, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycobiology 19:479-87. 2009
    ..We also found that the serum level of ST6Gal I in hepatitis C patients was correlated with the activity of hepatic inflammation...
  3. ncbi request reprint Involvement of proteases in glycosyltransferase secretion: Alzheimer's beta-secretase-dependent cleavage and a following processing by an aminopeptidase
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Frontier Research System, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycoconj J 21:25-9. 2004
    ..Taken together, we concluded that BACE1 initially cleaved ST6Gal I between Leu(37) and Gln(38), and the NH(2)-terminal three amino acids of the yielded product was further trimmed by the aminopeptidase...
  4. ncbi request reprint Beta-galactoside alpha2,6-sialyltransferase I cleavage by BACE1 enhances the sialylation of soluble glycoproteins. A novel regulatory mechanism for alpha2,6-sialylation
    Ichiro Sugimoto
    Glyco chain Functions Laboratory, Institute of Physical and Chemical Research, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 282:34896-903. 2007
    ..We propose a novel regulatory mechanism in which cleavage and secretion of ST6Gal I enhance the sialylation of soluble glycoprotein substrates...
  5. ncbi request reprint In vivo cleavage of alpha2,6-sialyltransferase by Alzheimer beta-secretase
    Shinobu Kitazume
    Glyco chain Functions Laboratory, The Institute of Physical and Chemical Research, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 280:8589-95. 2005
    ....
  6. ncbi request reprint Characterization of alpha 2,6-sialyltransferase cleavage by Alzheimer's beta -secretase (BACE1)
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Supra biomolecular System Group, Frontier Research System, Brain Science Institute, Institute of Physical and Chemical Research, RIKEN, Saitama 51 0198, Japan
    J Biol Chem 278:14865-71. 2003
    ..These results suggest that ST6Gal I is cleaved initially between Leu(37) and Gln(38) by BACE1, and then the three-amino acid sequence at the NH(2) terminus is removed by exopeptidase(s) before secretion from the cells...
  7. pmc Alpha2,6-sialic acid on platelet endothelial cell adhesion molecule (PECAM) regulates its homophilic interactions and downstream antiapoptotic signaling
    Shinobu Kitazume
    Disease Glycomics Team, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 285:6515-21. 2010
    ..The present findings open up a new possibility that modulation of glycosylation could be one of the promising strategies for regulating angiogenesis...
  8. ncbi request reprint Interleukin-1 beta up-regulates TACE to enhance alpha-cleavage of APP in neurons: resulting decrease in Abeta production
    Yuriko Tachida
    Glyco chain Functions Laboratory, Supra biomolecular System Group, Frontier Research System, The Institute of Physical and Chemical Research RIKEN, Saitama, Japan
    J Neurochem 104:1387-93. 2008
    ..Taken together we conclude that IL-1beta is an anti-amyloidogenic factor, and that enhancement of its signaling or inhibition of IL-1Ra activity could represent potential therapeutic strategies against Alzheimer's disease...
  9. ncbi request reprint Sialylation enhances the secretion of neurotoxic amyloid-beta peptides
    Kazuhiro Nakagawa
    Glycochain Functions Laboratory, Suprabiomolecular System Group, Frontier Research System, RIKEN, Wako Shi, Saitama, Japan
    J Neurochem 96:924-33. 2006
    ..In the mouse brain, the amount of alpha2,6-sialylated APP appeared to be correlated with the sAPPbeta level. These results suggest that sialylation of APP promotes its metabolic turnover and could affect the pathology of AD...
  10. pmc Brain endothelial cells produce amyloid {beta} from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form
    Shinobu Kitazume
    Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 285:40097-103. 2010
    ..Because we were able to detect O-glycosylated sAPP770β in human cerebrospinal fluid, this unique soluble APP770β has the potential to serve as a marker for cortical dementias such as AD and vascular dementia...
  11. ncbi request reprint Screening a series of sialyltransferases for possible BACE1 substrates
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Supra biomolecular System Group, Frontier Research System, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycoconj J 23:437-41. 2006
    ..These results suggest that BACE1 expression affects glycosylation not only by directly cleaving glycosyltransferases but also by modifying the secretion of glycosyltransferases via some other mechanisms...
  12. ncbi request reprint Alzheimer's beta-secretase cleaves a glycosyltransferase as a physiological substrate
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Frontier Research System and Laboratory for Proteolytic Neuroscience, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Glycoconj J 20:59-62. 2004
    ..Thus BACE1 is the first identified protease that is responsible for the cleavage and secretion of glycosyltransferases...
  13. pmc Loss of Siglec-14 reduces the risk of chronic obstructive pulmonary disease exacerbation
    Takashi Angata
    Systems Glycobiology Research Group, and RIKEN Max Planck Joint Research Center, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Cell Mol Life Sci 70:3199-210. 2013
    ..Taken together, Siglec-14 and its downstream signaling pathway facilitate the "infection-inflammation-exacerbation" axis of COPD disease progression, and may represent promising targets for therapeutic intervention. ..
  14. doi request reprint Loss of branched O-mannosyl glycans in astrocytes accelerates remyelination
    Kenji Kanekiyo
    Disease Glycomics Team, Systems Glycobiology Research Group, RIKEN MAX Planck Joint Research Center for Systems Chemical Biology, Global Research Cluster, RIKEN, Saitama 351 0198, Japan
    J Neurosci 33:10037-47. 2013
    ....
  15. pmc Mass isotopomer analysis of metabolically labeled nucleotide sugars and N- and O-glycans for tracing nucleotide sugar metabolisms
    Kazuki Nakajima
    Disease Glycomics Team, Systems Glycobiology Research Group, Global Research Cluster, RIKEN Max Plank Joint Research Center, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Mol Cell Proteomics 12:2468-80. 2013
    ..This indicates that metabolic flows are responsible for the low sialylation in the insulinoma cells. Our strategy should be useful for systematically tracing each stage of cellular GlcNAc metabolism. ..
  16. ncbi request reprint [Alzheimer's disease and glycosyltransferase]
    Shinobu Kitazume
    Glyco chain Functions Lab Frontier Research System, RIKEN Institute, 2 1, Hirosawa, Wako City, Saitama 351 0198, Japan
    Seikagaku 78:383-91. 2006
  17. ncbi request reprint [Identification of a novel substrate of Alzheimer's beta-secretase: beta-secretase dependent cleavage of alpha 2,6 sialyltransferase]
    Shinobu Kitazume
    Glyco chain Functions Laboratory, Frontier Research System, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198
    Seikagaku 74:1180-3. 2002
  18. ncbi request reprint Characterization of Drosophila aspartic proteases that induce the secretion of a Golgi-resident transferase, heparan sulfate 6-O-sulfotransferase
    Norihiro Kotani
    Glyco chain Functions Laboratory, Frontier Research System, The Institute of Physical and Chemical Research, RIKEN, Wako, Saitama 351 0198
    J Biochem 137:315-22. 2005
    ....
  19. ncbi request reprint Polyamine modification by acrolein exclusively produces 1,5-diazacyclooctanes: a previously unrecognized mechanism for acrolein-mediated oxidative stress
    Ayumi Tsutsui
    Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Org Biomol Chem 12:5151-7. 2014
    ..This study suggests that diazacyclooctane formation is involved in the mechanism underlying acrolein-mediated oxidative stress. ..
  20. doi request reprint Integrated approach toward the discovery of glyco-biomarkers of inflammation-related diseases
    Takashi Angata
    Systems Glycobiology Research Group, Chemical Biology Department, RIKEN Advanced Science Institute, Wako, Saitama, Japan
    Ann N Y Acad Sci 1253:159-69. 2012
    ..Future integration of large-scale "omics"-type data (e.g., genomics, epigenomics, transcriptomics, proteomics, and glycomics) with computational model building, or a systems glycobiology approach, will facilitate such efforts...
  21. doi request reprint A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells
    Katsunori Tanaka
    Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Org Biomol Chem 12:1412-8. 2014
    ..The probe was linked to a cyclic RGDyK peptide to enable the selective visualization of integrin αVβ3 on the surfaces of live cells. ..
  22. pmc Brain-specific expression of N-acetylglucosaminyltransferase IX (GnT-IX) is regulated by epigenetic histone modifications
    Yasuhiko Kizuka
    Disease Glycomics Team, Systems Glycobiology Research Group, Advanced Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 286:31875-84. 2011
    ..This is the first report demonstrating a molecular mechanism at the chromatin level underlying tissue-specific glycan expression...
  23. ncbi request reprint [Glycosyltransferases as substrates for Alzheimer's beta-secretase]
    Shinobu Kitazume
    Tanpakushitsu Kakusan Koso 49:2468-72. 2004
  24. ncbi request reprint Regulation of heparan sulfate 6-O-sulfation by beta-secretase activity
    Naoko Nagai
    Institute for Molecular Science of Medicine, Aichi Medical University, Yazako, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 282:14942-51. 2007
    ..Thus, the HS6ST3 enzyme in the Golgi apparatus and therefore the 6-O sulfation of heparan sulfates in the cell are at least partly regulated by beta-secretase via an indirect mechanism...
  25. ncbi request reprint KMI-358 and KMI-370, highly potent and small-sized BACE1 inhibitors containing phenylnorstatine
    Tooru Kimura
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, Kyoto Pharmaceutical University, Yamashina ku, Kyoto 607 8412, Japan
    Bioorg Med Chem Lett 14:1527-31. 2004
    ..Using KMI-008 as a lead compound, a small-sized and highly potent BACE1 inhibitor KMI-370 (IC(50)=3.4 nM) was designed and synthesized...