Mitsunori Fukuda

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. ncbi Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution?
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 9:831-42. 2004
  2. ncbi Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:39673-8. 2002
  3. ncbi Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 365:173-80. 2002
  4. ncbi Molecular cloning and characterization of human, rat, and mouse synaptotagmin XV
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, 351 0198, Saitama, Japan
    Biochem Biophys Res Commun 306:64-71. 2003
  5. ncbi The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptotagmin I
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 366:681-7. 2002
  6. ncbi Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:30351-8. 2002
  7. ncbi The calcium-binding loops of the tandem C2 domains of synaptotagmin VII cooperatively mediate calcium-dependent oligomerization
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:29315-20. 2002
  8. ncbi Slac2-a/melanophilin contains multiple PEST-like sequences that are highly sensitive to proteolysis
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 279:22314-21. 2004
  9. ncbi Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:12432-6. 2002
  10. ncbi Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:39175-84. 2005

Detail Information

Publications89

  1. ncbi Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution?
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 9:831-42. 2004
    ..Based on these findings, I propose that the Rab3A-effector function of Rim during secretory vesicle exocytosis is limited to the short form of the mammalian Rim RBD alone...
  2. ncbi Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:39673-8. 2002
    ..In contrast, expression of Slp3-a, but not of Slp3-b lacking an N-terminal Rab27A-binding domain, promotes NPY secretion. These findings suggest that the Slp family controls regulated dense-core vesicle exocytosis via binding to Rab27A...
  3. ncbi Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 365:173-80. 2002
    ....
  4. ncbi Molecular cloning and characterization of human, rat, and mouse synaptotagmin XV
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, 351 0198, Saitama, Japan
    Biochem Biophys Res Commun 306:64-71. 2003
    ..g., lung and testis) and Syt XV-a C2 domains lack Ca(2+)-dependent phospholipid binding activity, Syt XV-a is classified as a non-neuronal, Ca(2+)-independent Syt...
  5. ncbi The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptotagmin I
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 366:681-7. 2002
    ..These results indicate that the C2A domain of Slp3 is an atypical Ca2+-/phospholipid-binding machine, compared with other C-type tandem C2 proteins...
  6. ncbi Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:30351-8. 2002
    ....
  7. ncbi The calcium-binding loops of the tandem C2 domains of synaptotagmin VII cooperatively mediate calcium-dependent oligomerization
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:29315-20. 2002
    ..Based on these results, we suggest that the linear Ca2+-dependent oligomer may be aligned at the fusion site between vesicles and plasma membrane and modulate Ca2+-regulated exocytosis by opening or dilating fusion pores...
  8. ncbi Slac2-a/melanophilin contains multiple PEST-like sequences that are highly sensitive to proteolysis
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 279:22314-21. 2004
    ..Our findings suggest that protein degradation of Slac2-a is an essential process for proper melanosome distribution in melanocytes...
  9. ncbi Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:12432-6. 2002
    ..The discovery that myosin Va indirectly recognizes its cargo through Slac2-a, a novel Rab27A/B effector, should shed light on molecular recognition of its specific cargo by class V myosin...
  10. ncbi Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:39175-84. 2005
    ..These results suggest that Slp4-a modulates amylase release from parotid acinar cells through interaction with syntaxin-2/3 on the apical plasma membrane...
  11. ncbi Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:4601-4. 2002
    ..Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis...
  12. ncbi Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:40118-24. 2002
    ..Based on these findings, I propose that SHD1 of the Slp and Slac2 families be referred to as RBD27 (Rab-binding domain specific for Rab27 isoforms)...
  13. ncbi Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:43096-103. 2002
    ....
  14. ncbi Missense mutations in the globular tail of myosin-Va in dilute mice partially impair binding of Slac2-a/melanophilin
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Cell Sci 117:583-91. 2004
    ..On the basis of our findings, we propose that myosin-Va interacts with Slac2-a.Rab27A complex on the melanosome via two distinct domains, both of which are essential for melanosome transport in melanocytes...
  15. ncbi Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 279:13065-75. 2004
    ....
  16. ncbi Synaptotagmin VII is targeted to dense-core vesicles and regulates their Ca2+ -dependent exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 279:52677-84. 2004
    ....
  17. ncbi Effect of forskolin on synaptotagmin IV protein trafficking in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama
    J Biochem 136:245-53. 2004
    ....
  18. ncbi [Role of synaptotagmin and its related molecules in regulated secretion]
    Mitsunori Fukuda
    Tanpakushitsu Kakusan Koso 49:2186-97. 2004
  19. ncbi Versatile role of Rab27 in membrane trafficking: focus on the Rab27 effector families
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biochem 137:9-16. 2005
    ....
  20. ncbi Molecular cloning, expression, and characterization of a novel class of synaptotagmin (Syt XIV) conserved from Drosophila to humans
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biochem 133:641-9. 2003
    ....
  21. ncbi Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 278:15390-6. 2003
    ..The results strongly indicate that interaction of Slp4-a with the GDP-bound form of Rab27A, not with syntaxin IA or Munc18-1, is the primary reason that Slp4-a expression inhibits dense core vesicle exocytosis in PC12 cells...
  22. ncbi Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 278:15373-80. 2003
    ..Based on these results, I propose that Rim, rabphilin, and Noc2 function differently in concert with functional group III and/or VIII Rab proteins, including Rab3 isoforms...
  23. ncbi Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 278:3220-6. 2003
    ..Our results suggest that Syt IV is a stimulus (e.g. NGF)-dependent regulator for exocytosis of dense-core vesicles...
  24. ncbi The N-terminal cysteine cluster is essential for membrane targeting of B/K protein
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 360:441-8. 2001
    ..Based on our findings, we propose that the N-terminal domain of B/K protein contains a novel cysteine-based protein motif that may allow B/K protein to localize in the trans-Golgi network...
  25. ncbi Expression of synaptotagmin I or II promotes neurite outgrowth in PC12 cells
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, 351 0198, Saitama, Japan
    Neurosci Lett 295:33-6. 2000
    ..These results indicate the Ca(2+)/phospholipid binding site of Syt I or II to be essential for neurite outgrowth...
  26. ncbi Calcium-dependent and -independent hetero-oligomerization in the synaptotagmin family
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, Hirosawa, Wako, Saitama 351 0198, Japan
    J Biochem 128:637-45. 2000
    ..Our data suggest that Ca(2+)-dependent and -independent hetero-oligomerization of synaptotagmins may create a variety of Ca(2+)-sensors...
  27. ncbi Distinct self-oligomerization activities of synaptotagmin family. Unique calcium-dependent oligomerization properties of synaptotagmin VII
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 275:28180-5. 2000
    ..g. Syt II) occurs only when the two molecules are tethered at the NH(2)-terminal domain...
  28. ncbi Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII)
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem J 354:249-57. 2001
    ..Since Syt XIII is expressed in various tissues outside the brain, Syt XIII may be involved in constitutive vesicle transport...
  29. ncbi A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms
    M Fukuda
    Developmental Neurobiology Laboratory, Brain Science Institute, Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 274:31428-34. 1999
    ..These results suggest that Syt VI and Syt VIDeltaTM may play distinct roles in vesicular trafficking...
  30. ncbi Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biochem Biophys Res Commun 281:1226-33. 2001
    ..In addition, Slp family proteins are differentially distributed in different mouse tissues and at different developmental stages...
  31. ncbi Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biochem Biophys Res Commun 283:513-9. 2001
    ..In addition, we show that the Slp2-a/c/d mRNAs are differentially distributed in different mouse tissues and at different stages of development, suggesting that these transcripts may be regulated by different promoters...
  32. ncbi A unique spacer domain of synaptotagmin IV is essential for Golgi localization
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, Hirosawa, Wako, Saitama, Japan
    J Neurochem 77:730-40. 2001
    ..e. induce crystalloid ER-like structures). On the basis of these results, we propose that the divergent spacer domain among synaptotagmin isoforms may contain certain signals that determine the final destination of each isoform...
  33. ncbi The C2A domain of double C2 protein gamma contains a functional nuclear localization signal
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 276:24441-4. 2001
    ..Our findings indicate that by changing the nature of the putative Ca2+ binding loops the C2 domain has more diversified function in cellular signaling than a simple Ca2+ binding motif...
  34. ncbi Distinct developmental expression of synaptotagmin I and IX in the mouse brain
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama, Japan
    Neuroreport 17:179-82. 2006
    ..These findings suggest that synaptotagmin IX regulates the transport of certain vesicles in the brain other than synaptic vesicles...
  35. ncbi Mechanism of the SDS-resistant synaptotagmin clustering mediated by the cysteine cluster at the interface between the transmembrane and spacer domains
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 276:40319-25. 2001
    ....
  36. ncbi Formation of crystalloid endoplasmic reticulum induced by expression of synaptotagmin lacking the conserved WHXL motif in the C terminus. Structural importance of the WHXL motif in the C2B domain
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 276:41112-9. 2001
    ..Our findings indicate that the conserved WHXL motif is important not only for protein interaction site but for proper folding of the C2B domain...
  37. ncbi Distinct Rab27A binding affinities of Slp2-a and Slac2-a/melanophilin: Hierarchy of Rab27A effectors
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 343:666-74. 2006
    ..4, 19.2, and 112 nM, respectively. Our data suggest that distinct Rab27A binding activities of Slac2-a and Slp2-a ensure the order (or hierarchy) of Rab27A effectors that sequentially function in melanosome transport in melanocytes...
  38. ncbi Functional analysis of Slac2-c/MyRIP as a linker protein between melanosomes and myosin VIIa
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:28015-22. 2005
    ..Based on these findings, we propose that Slac2-c acts as a functional myosin VIIa receptor and that the Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates the transport of retinal melanosomes in pigment epithelium cells...
  39. ncbi The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Mol Cell Biol 23:5245-55. 2003
    ..Our findings indicate that Slac2-a organizes actin-based melanosome transport in cooperation with Rab27A, myosin Va, and actin...
  40. ncbi Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 293:899-906. 2002
    ..The results suggest that Slp5 might be involved in Rab27A-dependent membrane trafficking in specific tissues...
  41. ncbi Synaptotagmin V is targeted to dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells
    Chika Saegusa
    Fukuda Initiative Research Unit and the Laboratory for Developmental Neurobiology, Brain Science Institute, Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:24499-505. 2002
    ..Based on these results, we propose that Syt V is a dense-core vesicle-specific Syt isoform that controls a specific type of Ca2+-regulated secretion...
  42. ncbi The polybasic sequence in the C2B domain of rabphilin is required for the vesicle docking step in PC12 cells
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama, Japan
    J Neurochem 100:770-9. 2007
    ..These results indicate that the polybasic sequence in the C2B domain functions as an effector domain for SNAP-25 and controls the number of 'releasable' vesicles docked to the plasma membrane...
  43. ncbi Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes
    Taruho S Kuroda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Nat Cell Biol 6:1195-203. 2004
    ..These results reveal the differential and sequential roles of Rab27A-binding proteins in melanosome transport in melanocytes...
  44. ncbi Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X
    M Fukuda
    Developmental Neurobiology Laboratory, Brain Science Institute, Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 274:31421-7. 1999
    ....
  45. ncbi Non-polarized distribution of synaptotagmin IV in neurons: evidence that synaptotagmin IV is not a synaptic vesicle protein
    Keiji Ibata
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN (The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    Neurosci Res 43:401-6. 2002
    ..These results suggest that Syt IV regulates non-polarized membrane trafficking in neurons, which may be involved in synaptic plasticity or neuronal development...
  46. ncbi The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:39253-9. 2005
    ..These findings suggest that rabphilin is involved in the docking step of regulated exocytosis in PC12 cells, possibly through interaction between the C2B domain and SNAP-25...
  47. ncbi Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion
    Chika Saegusa
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Arch Biochem Biophys 475:87-92. 2008
    ..Morphological analysis of fasted Slp1 knockout mice showed an increased number of zymogen granules in the pancreatic acinar cells, indicating that Slp1 is part of the machinery of amylase secretion by the exocrine pancreas...
  48. ncbi Identification of EPI64 as a GTPase-activating protein specific for Rab27A
    Takashi Itoh
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 281:31823-31. 2006
    ..This is the first report of screening for Rab27A-GAP based on functional interactions, and our screening methods can be applied for other uncharacterized TBC proteins...
  49. ncbi The secretory granule-associated protein CAPS2 regulates neurotrophin release and cell survival
    Tetsushi Sadakata
    Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama 351 0198, Japan
    J Neurosci 24:43-52. 2004
    ..Thus, we suggest that CAPS2 mediates the depolarization-dependent release of NT-3 and BDNF from granule cells, leading to regulation in cell differentiation and survival during cerebellar development...
  50. ncbi Functional diversity of C2 domains of synaptotagmin family. Mutational analysis of inositol high polyphosphate binding domain
    M Fukuda
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    J Biol Chem 270:26523-7. 1995
    ..These functional diversities of C2B domains suggest that not all synaptotagmins function as inositol high polyphosphate sensors at the synaptic vesicle...
  51. ncbi [Role of synaptotagmin and its related molecules in membrane trafficking]
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    Seikagaku 77:603-18. 2005
  52. ncbi Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity
    Takashi Itoh
    Fukuda Initiative Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    Genes Cells 11:1023-37. 2006
    ....
  53. ncbi Synaptotagmin VII modulates the kinetics of dense-core vesicle exocytosis in PC12 cells
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 12:511-9. 2007
    ..Our data indicate that Syt VII modulates the kinetics of Ca(2+)-dependent dense-core vesicle exocytosis in neuroendocrine PC12 cells, possibly by modulating fusion pore opening...
  54. ncbi The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN (The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
    Mol Biol Cell 17:2101-12. 2006
    ..Our findings reveal a novel docking machinery for dense-core vesicle exocytosis: Slp4-a simultaneously interacts with Rab27A and Munc18-1 on the dense-core vesicle and with syntaxin-1a in the plasma membrane...
  55. ncbi [Functional diversity of the synaptotagmin family]
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198
    Seikagaku 74:1472-5. 2002
  56. ncbi Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN (The Institute of Physical and Chemical Research, Wako, Saitama, Japan
    J Cell Sci 119:2196-203. 2006
    ..Our findings indicate that Rab3A and Rab27A cooperatively regulate docking step(s) of dense-core vesicles to the plasma membrane...
  57. ncbi Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases
    Mutsuko Kukimoto-Niino
    Systems and Structural Biology Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Structure 16:1478-90. 2008
    ..These findings provide the structural basis for the exclusive association of Slac2-a with the Rab27 subfamily, whereas rabphilin binds several subfamilies, including Rab3 and Rab27...
  58. ncbi The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain
    Taruho S Kuroda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, Saitama, Japan
    J Biol Chem 277:9212-8. 2002
    ..These results strongly indicated that the SHD of Slp1--3 and Slac2 functions as an in vivo Rab27A binding domain...
  59. ncbi Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells
    Chika Saegusa
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 11:623-31. 2006
    ..Based on these results, we propose that Slp2-a is part of the mucin secretory machinery in surface mucous cells of mouse stomach...
  60. ncbi SYNCRIP, a cytoplasmic counterpart of heterogeneous nuclear ribonucleoprotein R, interacts with ubiquitous synaptotagmin isoforms
    A Mizutani
    Laboratory for, the Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 275:9823-31. 2000
    ..These findings suggested that SYNCRIP was a target of ubiquitous type of Syts and implied the involvement of ubiquitous Syts in the regulation of dynamics of the cytoplasmic mRNA...
  61. ncbi RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    Biochem J 380:875-9. 2004
    ..The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells...
  62. ncbi Cholesterol controls lipid endocytosis through Rab11
    Miwa Takahashi
    Frontier Research System, RIKEN, Wako, Saitama, Japan
    Mol Biol Cell 18:2667-77. 2007
    ..These results thus suggest that cholesterol controls endocytic routes of a subset of membrane lipids through rab11...
  63. ncbi Regulation of AP-2-synaptotagmin interaction by inositol high polyphosphates
    A Mizutani
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    Biochem Biophys Res Commun 240:128-31. 1997
    ....
  64. ncbi Doc2gamma, a third isoform of double C2 protein, lacking calcium-dependent phospholipid binding activity
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biochem Biophys Res Commun 276:626-32. 2000
    ..These findings indicate that Doc2gamma may also function as an effector for Munc13-1 and that it may be involved in the regulation of vesicular trafficking...
  65. ncbi Mechanism of the calcium-dependent multimerization of synaptotagmin VII mediated by its first and second C2 domains
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 276:27670-6. 2001
    ..Our results suggest that Syt VII is a specific isoform that can cluster different Syt isoforms with two hands in response to Ca(2+)...
  66. ncbi Drosophila AD3 mutation of synaptotagmin impairs calcium-dependent self-oligomerization activity
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, Saitama, Japan
    FEBS Lett 482:269-72. 2000
    ..All of these findings strongly support the idea that self-oligomerization of Syt I or II is essential for neurotransmitter release in vivo...
  67. ncbi The function of inositol high polyphosphate binding proteins
    M Fukuda
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Ibaraki, Japan
    Bioessays 19:593-603. 1997
    ....
  68. ncbi Mutation of the pleckstrin homology domain of Bruton's tyrosine kinase in immunodeficiency impaired inositol 1,3,4,5-tetrakisphosphate binding capacity
    M Fukuda
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, 3 1 1 Koyadai, Tsukuba, Ibaraki 305, Japan
    J Biol Chem 271:30303-6. 1996
    ..Our data suggest that B cell differentiation is closely correlated with the IP4 binding capacity of the PH domain of Btk...
  69. ncbi Expression cloning and characterization of a cDNA encoding a novel membrane protein required for the formation of O-acetylated ganglioside: a putative acetyl-CoA transporter
    A Kanamori
    Laboratory for Cellular Glycobiology, The Institute of Physical and Chemical Research, Wako, Saitama, Japan
    Proc Natl Acad Sci U S A 94:2897-902. 1997
    ..Based on these results, the protein encoded by AT-1 is suggested to be an Ac-CoA transporter that is involved in the process of O-acetylation...
  70. ncbi Distinct phosphoinositide binding specificity of the GAP1 family proteins: characterization of the pleckstrin homology domains of MRASAL and KIAA0538
    T Minagawa
    International Joint Project Calcium Oscillation, Japan Science and Technology Corporation, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 288:87-90. 2001
    ..Our data indicate that the distinct phosphoinositide binding specificity of the PH domain is attributable to the distinct subcellular localization of the GAP1 family...
  71. ncbi Rab27 and its effectors in secretory granule exocytosis: a novel docking machinery composed of a Rab27.effector complex
    M Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Soc Trans 34:691-5. 2006
    ....
  72. ncbi A novel zinc finger protein, zic, is involved in neurogenesis, especially in the cell lineage of cerebellar granule cells
    J Aruga
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    J Neurochem 63:1880-90. 1994
    ..These findings suggest that zic is one of a number of nuclear factors involved in both differentiation in early development and maintenance of properties of the cerebellar granule cells...
  73. ncbi Genomic structures of synaptotagmin II protein: comparison of exon-intron organization of the synaptotagmin gene family
    M Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama, 351 0198, Japan
    Biochem Biophys Res Commun 270:528-32. 2000
    ..The only conserved feature among the synaptotagmin gene family seems to have a single exon that encodes the whole transmembrane domain...
  74. ncbi Slp1 and Slp2-a localize to the plasma membrane of CTL and contribute to secretion from the immunological synapse
    Oliver Holt
    Sir William Dunn School of Pathology, University of Oxford, South Parks Rd, Oxford, OX1 3RE, UK
    Traffic 9:446-57. 2008
    ..These results suggest that both Slp1 and Slp2-a may form part of a docking complex, capturing secretory lysosomes at the immunological synapse...
  75. ncbi Dvl regulates endo- and exocytotic processes through binding to synaptotagmin
    Shosei Kishida
    Department of Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, 1 2 3, Kasumi, Minami Ku, Hiroshima 734 8551, Japan
    Genes Cells 12:49-61. 2007
    ..Taken together, these results suggest that Dvl is involved in endo- and exocytotic processes through the binding to Syt I...
  76. ncbi Increased plasma membrane localization of O-glycosylation-deficient mutant of synaptotagmin I in PC12 cells
    Eiko Kanno
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi, Japan
    J Neurosci Res 86:1036-43. 2008
    ..The results indicate that O-glycosylation, not N-glycosylation, is partially involved in efficient targeting of Syt I protein to dense-core vesicles in PC12 cells...
  77. ncbi Analysis of the role of Rab27 effector Slp4-a/Granuphilin-a in dense-core vesicle exocytosis
    Mitsunori Fukuda
    Methods Enzymol 403:445-57. 2005
    ....
  78. ncbi Synaptotagmin V and IX isoforms control Ca2+ -dependent insulin exocytosis
    Mariella Iezzi
    Division of Clinical Biochemistry, Department of Internal Medicine, University Medical Center, 1211 Geneva 4, Switzerland
    J Cell Sci 117:3119-27. 2004
    ..Remarkably, hormone release in response to glucose was selectively and strongly reduced, indicating that Syt V and Syt IX are directly involved in the Ca2+ -dependent stimulation of exocytosis...
  79. ncbi Synaptotagmin IV regulates glial glutamate release
    Qi Zhang
    Department of Neuroscience, Room 215, Stemmler Hall, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 101:9441-6. 2004
    ....
  80. ncbi Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosis
    Xiaodong Zhang
    Department of Biochemistry, University of Wisconsin, Madison 53706, USA
    Neuron 34:599-611. 2002
    ..These results indicate that the Ca2+-dependent interaction of synaptotagmin with SNAP-25 is essential for the Ca2+-dependent triggering of membrane fusion...
  81. ncbi Synaptotagmin interaction with the syntaxin/SNAP-25 dimer is mediated by an evolutionarily conserved motif and is sensitive to inositol hexakisphosphate
    Colin Rickman
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 279:12574-9. 2004
    ..Our results shed new light on the functional importance of the conserved polybasic synaptotagmin motif, suggesting that synaptotagmin interacts with the t-SNARE dimer to up-regulate the probability of SNARE-mediated membrane fusion...
  82. ncbi Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets
    Ryutaro Shirakawa
    Department of Geriatric Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 279:10730-7. 2004
    ..Thus, Rab27 regulates the dense core granule secretion in platelets by employing its binding protein, Munc13-4...
  83. ncbi Plasmalemmal sealing of transected mammalian neurites is a gradual process mediated by Ca(2+)-regulated proteins
    Soonmoon Yoo
    Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555-0641, USA
    J Neurosci Res 74:541-51. 2003
    ....
  84. ncbi Involvement of the Rab27 binding protein Slac2c/MyRIP in insulin exocytosis
    Laurent Waselle
    Institut de Biologie Cellulaire et de Morphologie, University of Lausanne, 1005 Lausanne, Switzerland
    Mol Biol Cell 14:4103-13. 2003
    ....
  85. ncbi Vesicular reuptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse
    Rodolfo R Llinas
    Department of Physiology and Neuroscience, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 101:17855-60. 2004
    ..This set of results addresses the issue of the geometrical arrangement of the Ca(2+) sensor, allowing the C2B domain antibody to restrict Ca(2+)-dependent C2B self-oligomerization without modifying the Ca(2+)-dependent release process...
  86. ncbi Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules
    Yael Haberman
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Cell Sci 116:4307-18. 2003
    ..These results therefore indicate that Syt IX is involved in regulating transport from the ERC to the cell surface, and suggest that it may play a role in linking vesicles that exit the ERC with the microtubules network...
  87. ncbi Characterization of the molecular defects in Rab27a, caused by RAB27A missense mutations found in patients with Griscelli syndrome
    Philippe Bahadoran
    INSERM U385, Biologie et Physiopathologie de la Peau, Faculte de Medecine, Avenue de Valombrose, 06107, Nice Cedex 2, France
    J Biol Chem 278:11386-92. 2003
    ..Our results pointed to an unexpected complexity of Rab27a function and open the way to the search for new Rab27a effectors or regulators that control the transport of Rab27a-dependent vesicles...
  88. ncbi Identification and biochemical analysis of Slac2-c/MyRIP as a Rab27A-, myosin Va/VIIa-, and actin-binding protein
    Taruho S Kuroda
    Methods Enzymol 403:431-44. 2005
    ..e., PC12 cells and mouse melanocytes). In this chapter we describe the methods that have been used to analyze the protein-protein interactions of Slac2-c, specifically with Rab27A, myosin Va/VIIa, and actin...
  89. ncbi The small GTPase Rab27B regulates amylase release from rat parotid acinar cells
    Akane Imai
    Department of Biochemistry, The Nippon Dental University, School of Dentistry at Niigata, 1 8, Hamaura cho, Niigata 951 8580, Japan
    J Cell Sci 117:1945-53. 2004
    ..Our results indicate that the Rab27B-Slac2-c complex is an important constituent of secretory granule exocytosis in parotid acinar cells...