Mitsunori Fukuda


Affiliation: RIKEN Brain Science Institute
Country: Japan


  1. Fukuda M, Kuroda T. Missense mutations in the globular tail of myosin-Va in dilute mice partially impair binding of Slac2-a/melanophilin. J Cell Sci. 2004;117:583-91 pubmed
    ..On the basis of our findings, we propose that myosin-Va interacts with Slac2-a.Rab27A complex on the melanosome via two distinct domains, both of which are essential for melanosome transport in melanocytes. ..
  2. Fukuda M, Saegusa C, Kanno E, Mikoshiba K. The C2A domain of double C2 protein gamma contains a functional nuclear localization signal. J Biol Chem. 2001;276:24441-4 pubmed
    ..Our findings indicate that by changing the nature of the putative Ca2+ binding loops the C2 domain has more diversified function in cellular signaling than a simple Ca2+ binding motif. ..
  3. Fukuda M. Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J Biol Chem. 2003;278:15373-80 pubmed
    ..Based on these results, I propose that Rim, rabphilin, and Noc2 function differently in concert with functional group III and/or VIII Rab proteins, including Rab3 isoforms. ..
  4. Fukuda M, Yamamoto A. Effect of forskolin on synaptotagmin IV protein trafficking in PC12 cells. J Biochem. 2004;136:245-53 pubmed
  5. Fukuda M, Mikoshiba K. Expression of synaptotagmin I or II promotes neurite outgrowth in PC12 cells. Neurosci Lett. 2000;295:33-6 pubmed
    ..These results indicate the Ca(2+)/phospholipid binding site of Syt I or II to be essential for neurite outgrowth. ..
  6. Fukuda M, Kanno E, Satoh M, Saegusa C, Yamamoto A. Synaptotagmin VII is targeted to dense-core vesicles and regulates their Ca2+ -dependent exocytosis in PC12 cells. J Biol Chem. 2004;279:52677-84 pubmed
  7. Fukuda M. The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptotagmin I. Biochem J. 2002;366:681-7 pubmed
    ..These results indicate that the C2A domain of Slp3 is an atypical Ca2+-/phospholipid-binding machine, compared with other C-type tandem C2 proteins. ..
  8. Fukuda M, Kanno E, Ogata Y, Saegusa C, Kim T, Loh Y, et al. Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells. J Biol Chem. 2003;278:3220-6 pubmed
    ..Our results suggest that Syt IV is a stimulus (e.g. NGF)-dependent regulator for exocytosis of dense-core vesicles. ..
  9. Fukuda M, Mikoshiba K. The N-terminal cysteine cluster is essential for membrane targeting of B/K protein. Biochem J. 2001;360:441-8 pubmed
    ..Based on our findings, we propose that the N-terminal domain of B/K protein contains a novel cysteine-based protein motif that may allow B/K protein to localize in the trans-Golgi network. ..

More Information


  1. Fukuda M. Distinct developmental expression of synaptotagmin I and IX in the mouse brain. Neuroreport. 2006;17:179-82 pubmed
    ..These findings suggest that synaptotagmin IX regulates the transport of certain vesicles in the brain other than synaptic vesicles. ..
  2. Fukuda M, Mikoshiba K. Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins. Biochem Biophys Res Commun. 2001;281:1226-33 pubmed
    ..In addition, Slp family proteins are differentially distributed in different mouse tissues and at different developmental stages. ..
  3. Fukuda M, Itoh T. Slac2-a/melanophilin contains multiple PEST-like sequences that are highly sensitive to proteolysis. J Biol Chem. 2004;279:22314-21 pubmed
    ..Our findings suggest that protein degradation of Slac2-a is an essential process for proper melanosome distribution in melanocytes. ..
  4. Fukuda M, Mikoshiba K. A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms. J Biol Chem. 1999;274:31428-34 pubmed
    ..These results suggest that Syt VI and Syt VIDeltaTM may play distinct roles in vesicular trafficking. ..
  5. Fukuda M. Molecular cloning, expression, and characterization of a novel class of synaptotagmin (Syt XIV) conserved from Drosophila to humans. J Biochem. 2003;133:641-9 pubmed
  6. Fukuda M. Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A. J Biol Chem. 2002;277:40118-24 pubmed
    ..Based on these findings, I propose that SHD1 of the Slp and Slac2 families be referred to as RBD27 (Rab-binding domain specific for Rab27 isoforms). ..
  7. Fukuda M, Kanno E, Yamamoto A. Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. J Biol Chem. 2004;279:13065-75 pubmed
  8. Fukuda M. Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution?. Genes Cells. 2004;9:831-42 pubmed
    ..Based on these findings, I propose that the Rab3A-effector function of Rim during secretory vesicle exocytosis is limited to the short form of the mammalian Rim RBD alone. ..
  9. Fukuda M, Imai A, Nashida T, Shimomura H. Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J Biol Chem. 2005;280:39175-84 pubmed
    ..These results suggest that Slp4-a modulates amylase release from parotid acinar cells through interaction with syntaxin-2/3 on the apical plasma membrane. ..
  10. Fukuda M. Molecular cloning and characterization of human, rat, and mouse synaptotagmin XV. Biochem Biophys Res Commun. 2003;306:64-71 pubmed
    ..g., lung and testis) and Syt XV-a C2 domains lack Ca(2+)-dependent phospholipid binding activity, Syt XV-a is classified as a non-neuronal, Ca(2+)-independent Syt. ..
  11. Fukuda M, Kuroda T. Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va/VIIa, and actin. J Biol Chem. 2002;277:43096-103 pubmed
  12. Fukuda M, Kanno E, Saegusa C, Ogata Y, Kuroda T. Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J Biol Chem. 2002;277:39673-8 pubmed
    ..In contrast, expression of Slp3-a, but not of Slp3-b lacking an N-terminal Rab27A-binding domain, promotes NPY secretion. These findings suggest that the Slp family controls regulated dense-core vesicle exocytosis via binding to Rab27A. ..
  13. Fukuda M, Ogata Y, Saegusa C, Kanno E, Mikoshiba K. Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human. Biochem J. 2002;365:173-80 pubmed
  14. Fukuda M, Kowalchyk J, Zhang X, Martin T, Mikoshiba K. Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells. J Biol Chem. 2002;277:4601-4 pubmed
    ..Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis. ..
  15. Fukuda M, Kanno E, Ogata Y, Mikoshiba K. Mechanism of the SDS-resistant synaptotagmin clustering mediated by the cysteine cluster at the interface between the transmembrane and spacer domains. J Biol Chem. 2001;276:40319-25 pubmed
  16. Fukuda M, Saegusa C, Mikoshiba K. Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem Biophys Res Commun. 2001;283:513-9 pubmed
    ..In addition, we show that the Slp2-a/c/d mRNAs are differentially distributed in different mouse tissues and at different stages of development, suggesting that these transcripts may be regulated by different promoters. ..
  17. Fukuda M, Mikoshiba K. Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII). Biochem J. 2001;354:249-57 pubmed
    ..Since Syt XIII is expressed in various tissues outside the brain, Syt XIII may be involved in constitutive vesicle transport. ..
  18. Fukuda M, Mikoshiba K. Calcium-dependent and -independent hetero-oligomerization in the synaptotagmin family. J Biochem. 2000;128:637-45 pubmed
    ..Our data suggest that Ca(2+)-dependent and -independent hetero-oligomerization of synaptotagmins may create a variety of Ca(2+)-sensors. ..