Research Topics
Genomes and GenesSpecies | Kuniya AbeSummaryAffiliation: RIKEN Brain Science Institute Country: Japan Publications
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Detail Information
Publications
Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence-SNP analysisKuniya Abe
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305 0074, Japan
Genome Res 14:2439-47. 2004..2% of the genome showed an extremely low SNP rate (percentage identity = 0.9997); these sequences are likely derived from the molossinus genome...
Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probesRintaro Saito
Institute for Advanced Biosciences, Keio University, Tsuruoka 997 0017, Japan
BMC Med Genomics 4:42. 2011..However, the number of known sense-antisense pairs remains limited because currently available cDNA sequences represent only a fraction of the total number of transcripts comprising the transcriptome of each cell type...
Genome-wide analysis of expression modes and DNA methylation status at sense-antisense transcript loci in mouseYutaka Watanabe
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center BRC, RIKEN Tsukuba Institute, Ibaraki 305 0074, Japan
Genomics 96:333-41. 2010..This cross-strand relationship is not a privilege of imprinted genes but seems to occur widely in SATs...
RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryosShogo Matoba
RIKEN BioResource Center, Tsukuba, Ibaraki 305 0074, Japan
Proc Natl Acad Sci U S A 108:20621-6. 2011..Furthermore, our strategy provides a promising breakthrough for mammalian SCNT cloning, because RNAi treatment of oocytes is readily applicable to most mammal species...
Genetic differences among C57BL/6 substrainsKazuyuki Mekada
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
Exp Anim 58:141-9. 2009..These data will be useful for accurate genetic monitoring of genetically engineered mice with the C57BL/6 background...
A high-speed congenic strategy using first-wave male germ cellsNarumi Ogonuki
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
PLoS ONE 4:e4943. 2009..Even with marker-assisted selection, the so-called 'speed congenic strategy', it takes more than a year at N4 or N5...
Large-scale production of growing oocytes in vitro from neonatal mouse ovariesArata Honda
BioResource Center, RIKEN, Tsukuba, Ibaraki 305 0074, Japan
Int J Dev Biol 53:605-13. 2009..This culture system may provide a significant impetus to the development of new techniques for the efficient production of oocytes from fetal or neonatal ovaries, for research, clinical, and zoological purposes...
Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transferKimiko Inoue
BioResource Center, RIKEN, 305 0024 Tsukuba, Japan
Science 330:496-9. 2010..Thus, we have identified nonrandom reprogramming errors in mouse cloning that can be altered to improve the efficiency of SCNT methods...
Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profilingNathan Mise
Mammalian Cellular Dynamics, RIKEN BioResource Center, Tsukuba, Japan
Genes Cells 13:863-77. 2008..The relationships between PGCs and stem cells derived from embryos or germ cells are discussed in light of these findings...
Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injectionAkie Shinmen
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
Mol Reprod Dev 74:1081-8. 2007..Thus, ICSI using cryopreserved spermatozoa allows the efficient and immediate production of a number of F1 hybrid mice and ES cell lines, which can be used for polymorphic analysis of mouse genetics...
The mouse resources at the RIKEN BioResource centerAtsushi Yoshiki
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
Exp Anim 58:85-96. 2009..RIKEN BRC collaborates with FIMRe members to support Japanese scientists in the use of valuable mouse resources from around the world...
Highly parallel SNP genotyping reveals high-resolution landscape of mono-allelic Ube3a expression associated with locus-wide antisense transcriptionKoji Numata
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center, RIKEN Tsukuba Institute, Ibaraki, 305 0074, Japan
Nucleic Acids Res 39:2649-57. 2011..The present study highlights the importance of locus-wide competition between sense and antisense transcripts...
X chromosome reactivation initiates in nascent primordial germ cells in miceMichihiko Sugimoto
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, Japan
PLoS Genet 3:e116. 2007..5 PGCs, indicating that X reactivation in developing PGCs occurs over a prolonged period. These results set the reactivation timing much earlier than previously thought and suggest that X reactivation may involve slow passive steps...
Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cellsSatoru Kobayakawa
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Ten noudai, Tsukuba, Ibaraki 305 8572, Japan
Genes Cells 12:447-60. 2007..Thus, this experimental system should facilitate studies focusing on relationships between nuclear organization, epigenetic status and cell differentiation...
Molecular Identification of t(w5): Vps52 Promotes Pluripotential Cell Differentiation through Cell-Cell InteractionsMichihiko Sugimoto
Technology and Development Team for Mammalian Cellular Dynamics, RIKEN BioResource Center, Tsukuba, Ibaraki 305 00074, Japan
Cell Rep 2:1363-74. 2012..It is also required in the formation of embryonic structures at a later stage of development, revealing hitherto unknown functions of Vps52 in the development of a multicellular organism...
High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage deviceLiqin Cao
Technology and Development Team for Mammalian Cellular Dynamics, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
PLoS ONE 7:e33876. 2012..The microstage approach adds an exciting new technique to the in vivo imaging toolbox...
Identification of novel endogenous antisense transcripts by DNA microarray analysis targeting complementary strand of annotated genesKoji Numata
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center BRC, RIKEN Tsukuba Institute, Ibaraki 305 0074, Japan
BMC Genomics 10:392. 2009....
Normal embryonic and germ cell development in mice lacking alpha 1,3-fucosyltransferase IX (Fut9) which show disappearance of stage-specific embryonic antigen 1Takashi Kudo
Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory, Tsukuba, Ibaraki 305 8568, Japan
Mol Cell Biol 24:4221-8. 2004..Therefore, we conclude that expression of the SSEA-1 epitope in the developing mouse embryo is not essential for embryogenesis in vivo...
Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5Kuniya Abe
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Mamm Genome 15:265-76. 2004..Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for the t(w5) embryonic lesion...
Disclosing hidden transcripts: mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localizedHidenori Kiyosawa
Technology and Development Team for Mammalian Cellular Dynamics, BioResource Center (BRC, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, Japan 305-0074
Genome Res 15:463-74. 2005..The tendency that the SATs are often not polyadenylated is conserved, even in the randomly chosen SAT genes in the plant Arabidopsis thaliana. Such common characteristics imply general roles of the SATs in regulation of gene expression...
Birth of mice produced by germ cell nuclear transferHiromi Miki
RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
Genesis 41:81-6. 2005....
