Kozo Hirokawa

Summary

Affiliation: Research and Development Division
Country: Japan

Publications

  1. ncbi Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Prefecture 278 0037, Japan
    Biochim Biophys Acta 1597:271-9. 2002
  2. ncbi Enhancement of thermostability of fungal deglycating enzymes by directed evolution
    Kozo Hirokawa
    Research and Development Division, Kikkoman, 399 Noda, Noda City, Chiba Prefecture 278 0037, Japan
    Appl Microbiol Biotechnol 78:775-81. 2008
  3. ncbi An enzymatic method for the determination of hemoglobinA(1C)
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, 278 0037, Noda City, Chiba Pref, Japan
    Biotechnol Lett 27:963-8. 2005
  4. ncbi Enzymes used for the determination of HbA1C
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba Pref 278 0037, Japan
    FEMS Microbiol Lett 235:157-62. 2004
  5. ncbi Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, Noda City, Chiba Pref 278 0037, Japan
    Biochem Biophys Res Commun 311:104-11. 2003
  6. ncbi Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Pref 278 0037, Noda City, Japan
    Arch Microbiol 180:227-31. 2003
  7. ncbi Recombinant agrobacterium AgaE-like protein with fructosyl amino acid oxidase activity
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biosci Biotechnol Biochem 66:2323-9. 2002
  8. ncbi Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis
    Keiko Gomi
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda shi, 278 0037, Chiba, Japan
    Gene 294:157-66. 2002
  9. ncbi Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases
    Atsushi Ichiyanagi
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda shi, Chiba 278 0037, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:130-3. 2013
  10. ncbi Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver
    Yoshitaka Kondo
    Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, 35 2 Sakae cho, Itabashi ku, Tokyo 173 0015, Japan
    FEBS Lett 570:57-62. 2004

Collaborators

Detail Information

Publications10

  1. ncbi Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Prefecture 278 0037, Japan
    Biochim Biophys Acta 1597:271-9. 2002
    ..cruciata luciferase. The pH-dependence of the bioluminescence emission spectra was also examined. In contrast to wild-type firefly luciferases characterized to date, the mutant did not show the pH-dependent red spectrum shift...
  2. ncbi Enhancement of thermostability of fungal deglycating enzymes by directed evolution
    Kozo Hirokawa
    Research and Development Division, Kikkoman, 399 Noda, Noda City, Chiba Prefecture 278 0037, Japan
    Appl Microbiol Biotechnol 78:775-81. 2008
    ..The half-life of inactivation at 50 degrees C was 3.61-fold longer than that of the parent enzyme. These engineered fructosyl peptide oxidases will be useful for industrial application to clinical diagnosis...
  3. ncbi An enzymatic method for the determination of hemoglobinA(1C)
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, 278 0037, Noda City, Chiba Pref, Japan
    Biotechnol Lett 27:963-8. 2005
    ..The linear relationship was observed between the concentration of HbA(1C) and the absorbancy of fructosyl peptide oxidase reaction, hence this new method is a practical means for measuring HbA(1C.)...
  4. ncbi Enzymes used for the determination of HbA1C
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba Pref 278 0037, Japan
    FEMS Microbiol Lett 235:157-62. 2004
    ....
  5. ncbi Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, Noda City, Chiba Pref 278 0037, Japan
    Biochem Biophys Res Commun 311:104-11. 2003
    ....
  6. ncbi Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Pref 278 0037, Noda City, Japan
    Arch Microbiol 180:227-31. 2003
    ..This enzyme could be useful for the measurement of hemoglobin A(1C), the N-terminal valine residue of the beta-subunit of which is glycated...
  7. ncbi Recombinant agrobacterium AgaE-like protein with fructosyl amino acid oxidase activity
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biosci Biotechnol Biochem 66:2323-9. 2002
    ..These characteristics were similar to a fructosyl amino acid oxidase from Corynebacterium sp. strain 2-4-1, suggesting that these prokaryotic enzymes comprise a new family of fructosyl amino acid oxidases...
  8. ncbi Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis
    Keiko Gomi
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda shi, 278 0037, Chiba, Japan
    Gene 294:157-66. 2002
    ..G-LRE and H-LRE were successfully expressed under the control of the lac promoter in Escherichia coli...
  9. ncbi Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases
    Atsushi Ichiyanagi
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda shi, Chiba 278 0037, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:130-3. 2013
    ..00, b = 230.05, c = 47.27 Å, β = 116.99°. The crystals of CFP-T7 and EFP-T5M diffracted to 1.8 and 1.6 Å resolution, respectively...
  10. ncbi Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver
    Yoshitaka Kondo
    Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, 35 2 Sakae cho, Itabashi ku, Tokyo 173 0015, Japan
    FEBS Lett 570:57-62. 2004
    ..Consequently, a reduction of SMP30 expression might account for the age-associated deterioration of cellular functions and enhanced susceptibility to harmful stimuli in aged tissue...