Tamotsu Yoshimori

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. doi request reprint Toward unraveling membrane biogenesis in mammalian autophagy
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 20:401-7. 2008
  2. pmc An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7
    Hitomi Yamaguchi
    Structural Biology Center, National Institute of Genetics SOKENDAI, Mishima Shizuoka, Japan
    PLoS Pathog 5:e1000670. 2009
  3. doi request reprint Group a Streptococcus: a loser in the battle with autophagy
    Tamotsu Yoshimori
    Department of Cell Regulation, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565 0871, Japan
    Curr Top Microbiol Immunol 335:217-26. 2009
  4. ncbi request reprint How autophagy saves mice: A cell-autonomous defense system against Sindbis virus infection
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Cell Host Microbe 7:83-4. 2010
  5. pmc Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L
    Kohichi Matsunaga
    Department of Genetics, Graduate School of Medicine, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Cell Biol 190:511-21. 2010
  6. pmc Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response
    Tatsuya Saitoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 106:20842-6. 2009
  7. pmc Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury
    Ikuko Maejima
    Department of Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan
    EMBO J 32:2336-47. 2013
  8. doi request reprint Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages
    Kohichi Matsunaga
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Nat Cell Biol 11:385-96. 2009
  9. pmc Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes
    Nobumichi Furuta
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka 565 0871, Japan
    Mol Biol Cell 21:1001-10. 2010
  10. pmc Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin
    Naonobu Fujita
    Department of Genetics, Graduate School of Medicine, 2 Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, 3 Department of Molecular Virology, Research Institute for Microbial Diseases, 4 Department of Host Defense, WPI Immunology Frontier Research Center, 5 Department of Host Defense, Research Institute for Microbial Disease, and 6 Core Instrumentation Facility, Research Institute for Microbial Disease, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Cell Biol 203:115-28. 2013

Detail Information

Publications85

  1. doi request reprint Toward unraveling membrane biogenesis in mammalian autophagy
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 20:401-7. 2008
    ....
  2. pmc An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7
    Hitomi Yamaguchi
    Structural Biology Center, National Institute of Genetics SOKENDAI, Mishima Shizuoka, Japan
    PLoS Pathog 5:e1000670. 2009
    ..Our results demonstrate that mechanisms of GcAV formation includes not only the common machinery of autophagy, but also Rab7 as an additional component, which is dispensable in canonical autophagosome formation...
  3. doi request reprint Group a Streptococcus: a loser in the battle with autophagy
    Tamotsu Yoshimori
    Department of Cell Regulation, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565 0871, Japan
    Curr Top Microbiol Immunol 335:217-26. 2009
    ..The role of autophagy in Staphylococcus aureus infection is also discussed...
  4. ncbi request reprint How autophagy saves mice: A cell-autonomous defense system against Sindbis virus infection
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Cell Host Microbe 7:83-4. 2010
    ....
  5. pmc Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L
    Kohichi Matsunaga
    Department of Genetics, Graduate School of Medicine, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Cell Biol 190:511-21. 2010
    ..The Atg14L-dependent appearance of PI3P in the ER makes this organelle the platform for autophagosome formation...
  6. pmc Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response
    Tatsuya Saitoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 106:20842-6. 2009
    ..These results demonstrate that dynamic membrane traffic mediates the sequential translocation and assembly of STING, both of which are essential processes required for maximal activation of the innate immune response triggered by dsDNA...
  7. pmc Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury
    Ikuko Maejima
    Department of Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan
    EMBO J 32:2336-47. 2013
    ..Thus, we propose that sequestration of damaged lysosomes by autophagy is indispensable for cellular and tissue homeostasis. ..
  8. doi request reprint Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages
    Kohichi Matsunaga
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Nat Cell Biol 11:385-96. 2009
    ..These data suggest that the Beclin 1-hVps34 complex functions in two different steps of autophagy by altering the subunit composition...
  9. pmc Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes
    Nobumichi Furuta
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka 565 0871, Japan
    Mol Biol Cell 21:1001-10. 2010
    ..These findings strongly suggest that the combinational SNARE proteins VAMP8 and Vti1b mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation...
  10. pmc Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin
    Naonobu Fujita
    Department of Genetics, Graduate School of Medicine, 2 Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, 3 Department of Molecular Virology, Research Institute for Microbial Diseases, 4 Department of Host Defense, WPI Immunology Frontier Research Center, 5 Department of Host Defense, Research Institute for Microbial Disease, and 6 Core Instrumentation Facility, Research Institute for Microbial Disease, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Cell Biol 203:115-28. 2013
    ..Thus, we reveal that ubiquitin is a pivotal molecule that connects bacteria-containing endosomes with the autophagic machinery upstream of LC3. ..
  11. doi request reprint Autophagosomes form at ER-mitochondria contact sites
    Maho Hamasaki
    Department of Genetics, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nature 495:389-93. 2013
    ..These results provide new insight into organelle biogenesis by demonstrating that the ER-mitochondria contact site is important in autophagosome formation...
  12. pmc Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus
    Shuhei Taguwa
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3 1, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    J Virol 85:13185-94. 2011
    ..These findings reveal a unique survival strategy of HCV and provide new insights into the genotype-specific pathogenicity of HCV...
  13. pmc An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 19:4651-9. 2008
    ..These results show that the lipidation of LC3 paralogues is involved in the completion of autophagosome formation in mammalian cells. This study also provides a powerful tool for a wide variety of studies of autophagy in the future...
  14. pmc The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella
    Shun Kageyama
    Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 22:2290-300. 2011
    ..We propose that the LC3 system is recruited through a mechanism that is independent of isolation membrane generation...
  15. pmc Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain
    Keisuke Tabata
    Department of Genetics, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 21:4162-72. 2010
    ..Furthermore, we show that Rubicon but not PLEKHM1 uniquely regulates membrane trafficking via simultaneously binding both Rab7 and PI3-kinase...
  16. ncbi request reprint TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy
    Kanae Shirahama-Noda
    Center for Frontier Oral Science, Graduate School of Dentistry, Osaka University, Osaka University, 565 0871 Osaka, Japan
    J Cell Sci 126:4963-73. 2013
    ..This clarification of the role of TRAPPIII elucidates how general membrane traffic contributes to autophagy...
  17. ncbi request reprint Atg14L recruits PtdIns 3-kinase to the ER for autophagosome formation
    Takeshi Noda
    Department of Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka Japan
    Autophagy 7:438-9. 2011
    ..In other words, this situation provides the ER with the opportunity to recruit whatever proteins are in demand. Recently, we have uncovered how PtdIns(3)P is generated on the ER for the autophagic process...
  18. pmc Differential involvement of Atg16L1 in Crohn disease and canonical autophagy: analysis of the organization of the Atg16L1 complex in fibroblasts
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 284:32602-9. 2009
    ..Therefore, we propose that Atg16L1 T300A is differentially involved in Crohn disease and canonical autophagy...
  19. ncbi request reprint Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation
    Mitsuko Hayashi-Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
    Autophagy 6:301-3. 2010
    ..Electron tomography revealed the ER-IM complex as a subdomain of the ER forming a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected...
  20. doi request reprint A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
    Mitsuko Hayashi-Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nat Cell Biol 11:1433-7. 2009
    ..Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected...
  21. doi request reprint Modulation of local PtdIns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy
    Naoko Taguchi-Atarashi
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Traffic 11:468-78. 2010
    ..Local levels of PtdIns3P at the site of autophagosome formation determine autophagy initiation and the size of the autophagosome membrane structure...
  22. ncbi request reprint Autophagy defends cells against invading group A Streptococcus
    Ichiro Nakagawa
    Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, 1 8 Yamadaoka, Suita Osaka 565 0871, Japan
    Science 306:1037-40. 2004
    ..In autophagy-deficient Atg5-/- cells, GAS survived, multiplied, and were released from the cells. Thus, the autophagic machinery can act as an innate defense system against invading pathogens...
  23. ncbi request reprint Between canonical and antibacterial autophagy: Rab7 is required for GAS-containing autophagosome-like vacuole formation
    Takeshi Noda
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 6:419-20. 2010
    ..We also showed that homotypic fusion of the initial GcAV leads to the formation of large GcAV, which is also unobserved in canonical autophagy...
  24. ncbi request reprint Mediatory molecules that fuse autophagosomes and lysosomes
    Nobumichi Furuta
    Department of Oral Frontier Biology, Graduate School of Dentistry, Osaka University, Yamadaoka, Suita Osaka, Japan
    Autophagy 6:417-8. 2010
    ..In addition, knockdown of these SNAREs inhibits the fusion of canonical autophagosomes with lysosomes. Together, these findings indicate that VAMP8 and Vti1b mediate fusion with lysosomes in both antimicrobial and canonical autophagy...
  25. doi request reprint Molecular basis of canonical and bactericidal autophagy
    Takeshi Noda
    Department of Cellular Regulation, Division of Cellular and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Int Immunol 21:1199-204. 2009
    ..PI3P is a key determinant of the autophagic membrane. Further, their relation to bactericidal autophagy (i.e. xenophagy) will be introduced...
  26. doi request reprint Monitoring autophagy in mammalian cultured cells through the dynamics of LC3
    Shunsuke Kimura
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Methods Enzymol 452:1-12. 2009
    ..These methods will provide a relatively easy assessment of autophagy, and the details of the procedure will be described along with possible pitfalls...
  27. ncbi request reprint Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  28. doi request reprint Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production
    Tatsuya Saitoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Nature 456:264-8. 2008
    ..These results demonstrate that Atg16L1 is an essential component of the autophagic machinery responsible for control of the endotoxin-induced inflammatory immune response...
  29. pmc The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 19:2092-100. 2008
    ..We propose that the Atg16L complex is a new type of E3-like enzyme that functions as a scaffold for LC3 lipidation by dynamically localizing to the putative source membranes for autophagosome formation...
  30. doi request reprint Inhibition of autophagy potentiates the antitumor effect of the multikinase inhibitor sorafenib in hepatocellular carcinoma
    Satoshi Shimizu
    Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Int J Cancer 131:548-57. 2012
    ..Concomitant inhibition of autophagy may be an attractive strategy for unlocking the antitumor potential of sorafenib in HCC...
  31. doi request reprint Regulation of membrane biogenesis in autophagy via PI3P dynamics
    Takeshi Noda
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka, Japan
    Semin Cell Dev Biol 21:671-6. 2010
    ..Recent developments in this field suggest that the local PI3P concentration is dynamically regulated during autophagy, and that this molecule is critical to the proper control of autophagy...
  32. ncbi request reprint The Ubi brothers reunited
    Takeshi Noda
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Autophagy 4:540-1. 2008
    ..This finding will provide further important clues as to the origin of the autophagosome membrane, and how the process is regulated by starvation and PtdIns3P signals...
  33. ncbi request reprint Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Struct Funct 33:109-22. 2008
    ..Collectively, our data demonstrate the functional significance of autophagosome movement that enables effective delivery from the cytosol to lysosomes...
  34. pmc Porphyromonas gingivalis outer membrane vesicles enter human epithelial cells via an endocytic pathway and are sorted to lysosomal compartments
    Nobumichi Furuta
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, 1 8 Yamadaoka, Suita Osaka 565 0871, Japan
    Infect Immun 77:4187-96. 2009
    ..gingivalis MVs efficiently enter host cells via an endocytic pathway and survive within the endocyte organelles for an extended period, which provides better understanding of the role of MVs in the etiology of periodontitis...
  35. ncbi request reprint Binding Rubicon to cross the Rubicon
    Kohichi Matsunaga
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 5:876-7. 2009
    ..Thus, the Beclin 1-Vps34-Vps15 complex functions in autophagy and the endocytic pathway, but its function in a given context depends on the identity of its interacting subunits...
  36. pmc Cochaperone activity of human butyrate-induced transcript 1 facilitates hepatitis C virus replication through an Hsp90-dependent pathway
    Shuhei Taguwa
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Suita Shi, Osaka, Japan
    J Virol 83:10427-36. 2009
    ..These results suggest that an Hsp90-dependent chaperone pathway incorporating hB-ind1 is involved in protein folding in the membranous web for the circumvention of the UPR and that it facilitates HCV replication...
  37. pmc Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation
    Kyohei Umebayashi
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 19:3454-62. 2008
    ..We propose that sustained tyrosine phosphorylation of EGFR facilitates its polyubiquitination in endosomes and counteracts rapid deubiquitination, thereby ensuring Hrs-dependent lysosomal sorting...
  38. doi request reprint Ubiquitination-mediated autophagy against invading bacteria
    Naonobu Fujita
    Department of Genetics, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 23:492-7. 2011
    ..Thus, ubiquitination seems to function as a general tag for selective autophagy in mammalian cells. This review discusses the present model of how autophagy sequesters invading bacteria through ubiquitination...
  39. doi request reprint Where do they come from? Insights into autophagosome formation
    Maho Hamasaki
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    FEBS Lett 584:1296-301. 2010
    ..Here, we present a comprehensive overview of the putative membrane sources identified to date...
  40. ncbi request reprint Autophagy in innate immunity against intracellular bacteria
    Atsuo Amano
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka
    J Biochem 140:161-6. 2006
    ..aureus were resistant to autophagic degradation. The present review focuses on the protective function of autophagy against bacterial invasion of cells...
  41. doi request reprint Up-to-date membrane biogenesis in the autophagosome formation
    Maho Hamasaki
    Department of Genetics, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 25:455-60. 2013
    ....
  42. pmc Critical roles for lipomannan and lipoarabinomannan in cell wall integrity of mycobacteria and pathogenesis of tuberculosis
    Takeshi Fukuda
    Research Institute for Microbial Diseases, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    MBio 4:e00472-12. 2013
    ..These findings will now allow us to seek the possibility that the LM/LAM biosynthetic pathway is a chemotherapeutic target...
  43. doi request reprint Autophagy guards against cisplatin-induced acute kidney injury
    Atsushi Takahashi
    Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Am J Pathol 180:517-25. 2012
    ..Enhancing autophagy may provide a novel therapeutic option to minimize AKI...
  44. pmc Three-Axis Model for Atg Recruitment in Autophagy against Salmonella
    Takeshi Noda
    Department of Genetics, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Int J Cell Biol 2012:389562. 2012
    ..We propose a novel model in which the mechanism of LC3 recruitment is separate from the generation of the isolation membrane. There exist at least three axes in Atg recruitment: ULK1 complex, Atg9L1, and Atg16L complex...
  45. ncbi request reprint Regulation of dsDNA-induced innate immune responses by membrane trafficking
    Tatsuya Saitoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan
    Autophagy 6:430-2. 2010
    ..These findings indicate that dynamic membrane trafficking is triggered by dsDNA stimulation and plays a pivotal role in the signal transduction required for optimal activation of the innate immune response...
  46. ncbi request reprint Atg4B(C74A) hampers autophagosome closure: a useful protein for inhibiting autophagy
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Autophagy 5:88-9. 2009
    ....
  47. ncbi request reprint Human RME-8 is involved in membrane trafficking through early endosomes
    Akemi Fujibayashi
    Division of Protein Chemistry, Institute for Protein Research, Osaka University, Suita, Osaka, Japan
    Cell Struct Funct 33:35-50. 2008
    ..Taken together, these results indicate that hRME is primarily involved in membrane trafficking through early endosomes, but not through degradative organelles, such as multivesicular bodies and late endosomes...
  48. ncbi request reprint Autophagy: paying Charon's toll
    Tamotsu Yoshimori
    Department of Cell Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita Osaka 565 0871, Japan
    Cell 128:833-6. 2007
    ..2007) analyze the role of autophagy in PCD during development. Unexpectedly they find that autophagy induces apoptotic cells to present a signal to ensure their clearance, which is crucial for mouse embryonic morphogenesis...
  49. ncbi request reprint Autophagy vs. Group A Streptococcus
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 2:154-5. 2006
    ..However, the autophagosomes induced by these bacteria have features distinct from those of the canonical pathway...
  50. pmc A current perspective of autophagosome biogenesis
    Shusaku T Shibutani
    1 Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan 2 Department of Genetics, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Cell Res 24:58-68. 2014
    ....
  51. ncbi request reprint Membrane recruitment of autophagy proteins in selective autophagy
    Eiji Morita
    Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Hepatol Res 42:435-41. 2012
    ..In this review, we summarize the current understanding of how ATG proteins behave during autophagosome formation in both non-selective and selective autophagy...
  52. ncbi request reprint [Cellular defense by autophagy]
    Tamotsu Yoshimori
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Gan To Kagaku Ryoho 35:21-4. 2008
    ..Therefore, autophagy is crucial for cellular defense against harmful materials inside cells...
  53. ncbi request reprint Autophagy: a regulated bulk degradation process inside cells
    Tamotsu Yoshimori
    Department of Cell Genetics, National Institute of Genetics, Mishima, Shizuoka ken 411 8540, Japan
    Biochem Biophys Res Commun 313:453-8. 2004
    ..Recent studies have revealed insights into the molecular basis of membrane dynamics and the regulation of autophagy, which had remained cryptic for a long time...
  54. pmc Inhibition of macroautophagy triggers apoptosis
    Patricia Boya
    CNRS UMR 8125, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Mol Cell Biol 25:1025-40. 2005
    ..All together, these data indicate that autophagy may be cytoprotective, at least under conditions of nutrient depletion, and point to an important cross talk between type 1 and type 2 cell death pathways...
  55. ncbi request reprint LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
    Yukiko Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji 38, Okazaki 444 8585, Japan
    J Cell Sci 117:2805-12. 2004
    ..Generation of the form II correlates with autophagosome association of GABARAP and GATE16. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II...
  56. ncbi request reprint Role of Hrs in maturation of autophagosomes in mammalian cells
    Keiichi Tamai
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Biochem Biophys Res Commun 360:721-7. 2007
    ..Based on these results, we propose a novel function of Hrs, as a crucial player in the maturation of autophagosomes...
  57. ncbi request reprint SKD1 AAA ATPase-dependent endosomal transport is involved in autolysosome formation
    Atsuki Nara
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji, Okazaki, Japan
    Cell Struct Funct 27:29-37. 2002
    ..These results indicate that SKD1-dependent endosomal membrane trafficking is required for formation of autolysosomes...
  58. ncbi request reprint Localization of mammalian NAD(P)H steroid dehydrogenase-like protein on lipid droplets
    Masato Ohashi
    Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444 8585, Japan
    J Biol Chem 278:36819-29. 2003
    ..These results add to the growing notion that the lipid droplet is an organelle endowed with more complex roles in various biological phenomena...
  59. ncbi request reprint Role of the Apg12 conjugation system in mammalian autophagy
    Noboru Mizushima
    PRESTO, Japan Science and Technology Corporation, 332 0012, Kawaguchi, Japan
    Int J Biochem Cell Biol 35:553-61. 2003
    ..Discovery of the Apg12 system has facilitated our understanding of the molecular mechanism of autophagosome formation...
  60. ncbi request reprint Autophagosome formation in mammalian cells
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki 444 8585, Japan
    Cell Struct Funct 27:421-9. 2002
    ..These findings are now being applied to studies on the physiological roles of autophagy in mammals...
  61. ncbi request reprint Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate
    Noboru Mizushima
    PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Sci 116:1679-88. 2003
    ..Because WD-repeat proteins provide a platform for protein-protein interactions, the approximately 800 kDa complex is expected to function in autophagosome formation, further interacting with other proteins in mammalian cells...
  62. ncbi request reprint The first molecular evidence that autophagy relates rimmed vacuole formation in chloroquine myopathy
    Takashi Suzuki
    Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro ku, Tokyo 153 8902
    J Biochem 131:647-51. 2002
    ..Therefore, we concluded that autophagy plays an important role in rimmed vacuole formation in certain muscular atrophies...
  63. ncbi request reprint Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method
    Noboru Mizushima
    PRESTO, Japan Science and Technology Corporation, 332 0012, Kawaguchi, Japan
    FEBS Lett 532:450-4. 2002
    ..This novel two-hybrid method, which we have named 'conjugation-mediated yeast two-hybrid', proves to be a simple and useful technique with which to analyze protein-protein conjugation...
  64. ncbi request reprint Toward and beyond lysosomes
    Tamotsu Yoshimori
    Department of Cell Genetics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka ken 411 8540, Japan
    Cell Struct Funct 27:401-2. 2002
  65. ncbi request reprint A dominant negative form of the AAA ATPase SKD1/VPS4 impairs membrane trafficking out of endosomal/lysosomal compartments: class E vps phenotype in mammalian cells
    Hideaki Fujita
    Graduate School of Pharmaceutical Sciences, Kyushu University 3 1 1, Maidashi, Higashi ku, Fukuoka, 812 8582, Japan
    J Cell Sci 116:401-14. 2003
    ..We conclude that SKD1 regulates multiple steps of membrane transport out of early endosomes and the reformation of lysosomes from a hybrid organelle...
  66. ncbi request reprint Functional analysis of alpha5beta1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivalis using fluorescent beads coated with bacterial membrane vesicles
    Kayoko Tsuda
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka, Japan
    Cell Struct Funct 33:123-32. 2008
    ..gingivalis adhesion to epithelial cells, while the subsequent uptake process requires lipid raft components for actin organization, with Rho GTPase Rac1...
  67. ncbi request reprint The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting
    Keiichi Katoh
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 278:39104-13. 2003
    ..These results suggest that CHMP4b and Alix participate in formation of multivesicular bodies by cooperating with SKD1...
  68. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
    ..Our results suggest that the regulation of autophagy is organ dependent and the role of autophagy is not restricted to the starvation response. This transgenic mouse model is a useful tool to study mammalian autophagy...
  69. ncbi request reprint GFP-like proteins stably accumulate in lysosomes
    Hiroyuki Katayama
    Laboratory for Cell Function Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN
    Cell Struct Funct 33:1-12. 2008
    ....
  70. ncbi request reprint How to interpret LC3 immunoblotting
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    Autophagy 3:542-5. 2007
    ..Accordingly, simple comparison of LC3-I and LC3-II, or summation of LC3-I and LC3-II for ratio determinations, may not be appropriate, and rather, the amount of LC3-II can be compared between samples...
  71. ncbi request reprint Nordihydroguaiaretic acid affects multiple dynein-dynactin functions in interphase and mitotic cells
    Kohei Arasaki
    School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Pharmacol 71:454-60. 2007
    ..To our knowledge, NDGA is the first case of a reagent that can modulate dynein-dynactin-related processes...
  72. ncbi request reprint Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype
    Takahiro Kato
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka, Japan
    Cell Microbiol 9:753-65. 2007
    ..In a mouse abscess model, the type II clones significantly induced serum IL-1beta and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence...
  73. ncbi request reprint [Host defense mechanism by autophagy]
    Ichiro Nakagawa
    Tanpakushitsu Kakusan Koso 51:1507-14. 2006
  74. ncbi request reprint ALIS are stress-induced protein storage compartments for substrates of the proteasome and autophagy
    Jason Szeto
    Infection, Immunity, Injury and Repair Program, The Hospital for Sick Children, Toronto, Ontario, Canada
    Autophagy 2:189-99. 2006
    ..Together, these findings demonstrate that ALIS act as generalized stress-induced protein storage compartments for substrates of the proteasome and autophagy...
  75. ncbi request reprint Survival of parvovirus B19-infected cells by cellular autophagy
    Akitoshi Nakashima
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Virology 349:254-63. 2006
    ..Moreover, inhibition of autophagy by 3-MA significantly facilitated B19-infection-mediated cell death. These results suggest a novel mechanism by which B19-infected cells survive by cellular autophagy...
  76. ncbi request reprint Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole
    Cheryl L Birmingham
    Infection, Immunity, Injury and Repair Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 281:11374-83. 2006
    ..Our findings support a role for autophagy in innate immunity and demonstrate that Salmonella infection is a powerful model to study the autophagy process...
  77. ncbi request reprint Molecular dissection of internalization of Porphyromonas gingivalis by cells using fluorescent beads coated with bacterial membrane vesicle
    Kayoko Tsuda
    Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920 1192, Japan
    Cell Struct Funct 30:81-91. 2005
    ..These results suggest that P. gingivalis accomplishes its internalization utilizing membrane lipid raft and cytoskeletal functions of the target cells...
  78. ncbi request reprint Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast
    Takahiro Yamada
    Program in Genetics and Genomic Biology, Research Institute, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:18283-90. 2005
    ..Moreover, APG9L2 is a vertebrate-specific gene that may have gained critical roles in mammalian-specific developmental events, such as placentation, through rapid evolution...
  79. ncbi request reprint Escape of intracellular Shigella from autophagy
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Science 307:727-31. 2005
    ..Rather, Shigella VirG, a protein required for intracellular actin-based motility, induced autophagy by binding to the autophagy protein, Atg5. In nonmutant Shigella, this binding is competitively inhibited by IcsB binding to VirG...
  80. ncbi request reprint The role of autophagy during the early neonatal starvation period
    Akiko Kuma
    Time s Arrow and Biosignaling, PRESTO, Japan Science and Technology Agency, Kawaguchi 332 0012, Japan
    Nature 432:1032-6. 2004
    ..These results suggest that the production of amino acids by autophagic degradation of 'self' proteins, which allows for the maintenance of energy homeostasis, is important for survival during neonatal starvation...
  81. ncbi request reprint Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes
    Anne Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo 0310, Norway
    J Cell Sci 117:4239-51. 2004
    ..By electron microscopy, similar structures can be found within autophagosomes. We propose that Alfy might target cytosolic protein aggregates for autophagic degradation...
  82. ncbi request reprint Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B
    Hideaki Fujita
    Division of Pharmaceutical Cell Biology, Kyushu University Graduate School of Pharmaceutical Sciences, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    J Cell Sci 117:2997-3009. 2004
    ..Intriguingly, the N-terminal coiled-coil region of mVps2 is required for the formation of the E235Q compartment but not for binding to SKD1. We propose that both SBP1 and mVps2 regulate SKD1 function in mammalian cells...
  83. ncbi request reprint [Autophagy as a bulk protein degradation system: it plays various roles]
    Tamotsu Yoshimori
    Tanpakushitsu Kakusan Koso 49:1029-32. 2004
  84. pmc CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis
    Jae Hyuck Shim
    Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 172:1045-56. 2006
    ..Therefore, CHMP5 regulates late endosome function downstream of MVB formation, and the loss of CHMP5 enhances signal transduction by inhibiting lysosomal degradation of activated receptors...
  85. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...