Affiliation: Osaka Medical Center for Cancer and Cardiovascular Diseases
Wada Y. Glycan profiling: label-free analysis of glycoproteins. Methods Mol Biol. 2013;951:245-53 pubmed publisher
..Glycopeptide is a suitable analyte for this label-free approach. ..
Tanaka K, Takenaka S, Tsuyama S, Wada Y. Determination of unique amino acid substitutions in protein variants by peptide mass mapping with FT-ICR MS. J Am Soc Mass Spectrom. 2006;17:508-513 pubmed publisher
..Therefore, FTICR MS is capable of identifying all 53 types of substitutions, each of which is associated with a unique mass difference, except for the Leu and Ile isomers. ..
Wada Y, Dell A, Haslam S, Tissot B, Canis K, Azadi P, et al
. Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1. Mol Cell Proteomics. 2010;9:719-27 pubmed publisher
..In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful. ..
Wada Y, Tajiri M, Ohshima S. Quantitation of saccharide compositions of O-glycans by mass spectrometry of glycopeptides and its application to rheumatoid arthritis. J Proteome Res. 2010;9:1367-73 pubmed publisher
Fujihara A, Inoue H, Sogi M, Tajiri M, Wada Y. Chiral and Molecular Recognition through Protonation between Aromatic Amino Acids and Tripeptides Probed by Collision-Activated Dissociation in the Gas Phase. Molecules. 2018;23: pubmed publisher
..The amino-acid sequences of the tripeptides required for the recognition of aromatic amino acids were determined by analyses of the CAD spectra. ..
Wada Y. Mass spectrometry of transferrin and apolipoprotein C-III for diagnosis and screening of congenital disorder of glycosylation. Glycoconj J. 2016;33:297-307 pubmed publisher
..In this technological review, the principle and pitfalls of MS for CDG are discussed and mass spectra of various types of CDG are presented. ..