Yoshinao Wada

Summary

Affiliation: Osaka Medical Center for Cancer and Cardiovascular Diseases
Country: Japan

Publications

  1. ncbi request reprint Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Glycobiology 17:411-22. 2007
  2. pmc Calponin 3 regulates actin cytoskeleton rearrangement in trophoblastic cell fusion
    Yukinao Shibukawa
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Mol Biol Cell 21:3973-84. 2010
  3. doi request reprint Glycan profiling: label-free analysis of glycoproteins
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Methods Mol Biol 951:245-53. 2013
  4. doi request reprint Mass spectrometry of apolipoprotein C-III, a simple analytical method for mucin-type O-glycosylation and its application to an autosomal recessive cutis laxa type-2 (ARCL2) patient
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Glycobiology 22:1140-4. 2012
  5. ncbi request reprint Technical aspects of gel-based proteomics designed for elucidating an aryl hydrocarbon receptor complex
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka 594 1101, Japan
    Environ Sci 11:25-31. 2004
  6. pmc Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Mol Cell Proteomics 9:719-27. 2010
  7. ncbi request reprint Mass spectrometry for congenital disorders of glycosylation, CDG
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka 594 1101, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 838:3-8. 2006
  8. ncbi request reprint Ordered porous alumina geometries and surface metals for surface-assisted laser desorption/ionization of biomolecules: possible mechanistic implications of metal surface melting
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Anal Chem 79:9122-7. 2007
  9. doi request reprint Quantitation of saccharide compositions of O-glycans by mass spectrometry of glycopeptides and its application to rheumatoid arthritis
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    J Proteome Res 9:1367-73. 2010
  10. ncbi request reprint Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    Anal Chem 76:6560-5. 2004

Detail Information

Publications44

  1. ncbi request reprint Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Glycobiology 17:411-22. 2007
    ....
  2. pmc Calponin 3 regulates actin cytoskeleton rearrangement in trophoblastic cell fusion
    Yukinao Shibukawa
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Mol Biol Cell 21:3973-84. 2010
    ..These results indicated that CNN3 regulates actin cytoskeleton rearrangement which is required for the plasma membranes of trophoblasts to become fusion competent...
  3. doi request reprint Glycan profiling: label-free analysis of glycoproteins
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Methods Mol Biol 951:245-53. 2013
    ..Glycopeptide is a suitable analyte for this label-free approach...
  4. doi request reprint Mass spectrometry of apolipoprotein C-III, a simple analytical method for mucin-type O-glycosylation and its application to an autosomal recessive cutis laxa type-2 (ARCL2) patient
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Glycobiology 22:1140-4. 2012
    ..It allows reproducible glycan profiling and detection of unglycosylated species. This method was applied to an autosomal recessive cutis laxa type-2 patient and demonstrated decreased site occupancy by O-glycosylation...
  5. ncbi request reprint Technical aspects of gel-based proteomics designed for elucidating an aryl hydrocarbon receptor complex
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka 594 1101, Japan
    Environ Sci 11:25-31. 2004
    ..There is also room for improvement in each step of sample preparation prior to mass spectrometry...
  6. pmc Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Mol Cell Proteomics 9:719-27. 2010
    ..In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful...
  7. ncbi request reprint Mass spectrometry for congenital disorders of glycosylation, CDG
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka 594 1101, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 838:3-8. 2006
    ..These techniques will facilitate studying CDG, a field now expanding to O-linked glycosylation and to acquired as well as inherited conditions that can affect protein glycosylation...
  8. ncbi request reprint Ordered porous alumina geometries and surface metals for surface-assisted laser desorption/ionization of biomolecules: possible mechanistic implications of metal surface melting
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Anal Chem 79:9122-7. 2007
    ..Activity is maintained for months in air...
  9. doi request reprint Quantitation of saccharide compositions of O-glycans by mass spectrometry of glycopeptides and its application to rheumatoid arthritis
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    J Proteome Res 9:1367-73. 2010
    ....
  10. ncbi request reprint Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    Anal Chem 76:6560-5. 2004
    ..The strategy, isolation of glycopeptides followed by MS(n) analysis, efficiently characterized the structures of beta2-glycoprotein I with four N-glycosylation sites and was applied to an analysis of total serum glycoproteins...
  11. ncbi request reprint Rock-dependent calponin 3 phosphorylation regulates myoblast fusion
    Yukinao Shibukawa
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Exp Cell Res 319:633-48. 2013
    ..CNN3 was also expressed and phosphorylated during myotube induction in isolated muscle satellite cells. Taken together, these results indicate that CNN3 is a downstream regulator of the ROCK signaling pathway for myogenesis...
  12. doi request reprint Dissociation profile of protonated fucosyl glycopeptides and quantitation of fucosylation levels of glycoproteins by mass spectrometry
    Michiko Tajiri
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka 594 1101, Japan
    J Proteome Res 8:688-93. 2009
    ....
  13. doi request reprint Site-specific analysis of N-glycans on haptoglobin in sera of patients with pancreatic cancer: a novel approach for the development of tumor markers
    Miyako Nakano
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka, Japan
    Int J Cancer 122:2301-9. 2008
    ..Thus, the present study provides evidence that site-specific analyses of N-glycans may be useful as novel tumor markers for PC...
  14. doi request reprint Calponin 3 regulates stress fiber formation in dermal fibroblasts during wound healing
    Etsuko Daimon
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Arch Dermatol Res 305:571-84. 2013
    ..These findings indicate that CNN3 participates in actin stress fiber remodeling, which is required for cell motility and contraction of dermal fibroblasts in the wound healing process. ..
  15. ncbi request reprint Oligosaccharide profiles of the prostate specific antigen in free and complexed forms from the prostate cancer patient serum and in seminal plasma: a glycopeptide approach
    Michiko Tajiri
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    Glycobiology 18:2-8. 2008
    ....
  16. doi request reprint Deficiency of N-acetylgalactosamine in O-linked oligosaccharides of IgA is a novel biologic marker for Crohn's disease
    Takahiro Inoue
    Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Inflamm Bowel Dis 18:1723-34. 2012
    ..We have reported that alteration of N-linked oligosaccharides of immunoglobulin (Ig) G is a novel diagnostic marker of IBD. Oligosaccharide alterations of IgA, however, have not been investigated in IBD patients...
  17. pmc Transglutaminase 2-dependent deamidation of glyceraldehyde-3-phosphate dehydrogenase promotes trophoblastic cell fusion
    Kaori Iwai
    From the Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan and
    J Biol Chem 289:4989-99. 2014
    ..Considering that GAPDH binds F-actin via an electrostatic interaction and that the cytoskeleton is rearranged in trophoblastic cell fusion, TG2-dependent GAPDH deamidation was suggested to participate in actin cytoskeletal remodeling. ..
  18. ncbi request reprint Bisecting GlcNAc mediates the binding of annexin V to Hsp47
    Cong Xiao Gao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Glycobiology 15:1067-75. 2005
    ..Collectively, these results suggest that the binding of annexin V to Hsp47 is mediated by a bisecting GlcNAc oligosaccharide structure and that Hsp47 is an intracellular ligand glycoprotein for annexin V...
  19. ncbi request reprint Mass spectrometry in the detection and diagnosis of congenital disorders of glycosylation
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Department of Molecular Medicine, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Eur J Mass Spectrom (Chichester, Eng) 13:101-3. 2007
    ..This program is anticipated to reveal the prevalence of CDG and to demonstrate the essential role of MS in the emerging field of medicine, disease glycomics and glycoproteomics...
  20. ncbi request reprint Transfer of maternal cholesterol to embryo and fetus in pregnant mice
    Shumi Yoshida
    Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    J Lipid Res 46:2168-74. 2005
    ..Intrauterine transfer of maternal cholesterol to the embryo as well as the fetus was evident in mice, and both the placenta and the yolk sac appear to be sites of intermediate passage in murine pregnancy...
  21. ncbi request reprint Advanced analytical methods for hemoglobin variants
    Yoshinao Wada
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 781:291-301. 2002
    ....
  22. doi request reprint High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides
    Shigeo Hayakawa
    Department of Chemistry, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka, Japan
    J Am Soc Mass Spectrom 21:1482-9. 2010
    ....
  23. ncbi request reprint Immunohistochemical distribution of Toll-like receptor 4 in term and preterm human placentas from normal and complicated pregnancy including chorioamnionitis
    Kaori Kumazaki
    Department of Molecual Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Hum Pathol 35:47-54. 2004
    ..Elucidation of biological functions of placental TLR4 under physiological conditions requires further investigation...
  24. ncbi request reprint Determination of unique amino acid substitutions in protein variants by peptide mass mapping with FT-ICR MS
    Katsuhiro Tanaka
    Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan
    J Am Soc Mass Spectrom 17:508-13. 2006
    ..Therefore, FTICR MS is capable of identifying all 53 types of substitutions, each of which is associated with a unique mass difference, except for the Leu and Ile isomers...
  25. doi request reprint Decreased sialylation of IgA1 O-glycans associated with pneumococcal hemolytic uremic syndrome
    Hisaaki Aoki
    Pediatric Department, Sakai Municipal Hospital, Osaka, Japan
    Pediatr Int 55:e143-5. 2013
    ..pneumonia-produced sialidases hydrolyzing both α2,3- and α2,6-linked sialic acids. The method will complement the T-antigen activation test and help to understand the molecular pathology related to P-HUS...
  26. ncbi request reprint Influence of metal-peptide complexation on fragmentation and inter-fragment hydrogen migration in electron transfer dissociation
    Daiki Asakawa
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    J Am Soc Mass Spectrom 25:1029-39. 2014
    ....
  27. ncbi request reprint Role of neutrophils in matrix metalloproteinase activity in the preimplantation mouse uterus
    Etsuko Daimon
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Japan
    Biol Reprod 73:163-71. 2005
    ....
  28. ncbi request reprint The N-terminal region of NTAK/neuregulin-2 isoforms has an inhibitory activity on angiogenesis
    Norihiko Nakano
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    J Biol Chem 279:11465-70. 2004
    ..These results suggest that the active site of NTAK is localized outside of the EGF-like domain but within the N-terminal region, including the Ig-like domain, of NTAK...
  29. ncbi request reprint Requirements for laser-induced desorption/ionization on submicrometer structures
    Shoji Okuno
    Wada Project Laboratory, Japan Science and Technology Agency, Innovation Plaza Osaka, 3 1 10 Technostage, Izumi, Osaka, Japan
    Anal Chem 77:5364-9. 2005
    ..These results indicate that laser-induced desorption/ionization of organic compounds can be implemented on submicrometer structures with an Au- or Pt-coated surface irrespective of the basal materials...
  30. ncbi request reprint Brd2 is required for cell cycle exit and neuronal differentiation through the E2F1 pathway in mouse neuroepithelial cells
    Mami Tsume
    Department of Molecular Embryology, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka Prefectural Hospital Organization, Izumi, Osaka 594 1101, Japan
    Biochem Biophys Res Commun 425:762-8. 2012
    ..These findings suggest that Brd2 is required for cell cycle exit and neuronal differentiation of neuroepithelial cells through the E2F1 pathway during mouse CNS development...
  31. doi request reprint Distinct features of matrix-assisted 6 microm infrared laser desorption/ionization mass spectrometry in biomolecular analysis
    Michiko Tajiri
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho Izumi, Osaka 594 1101, Japan
    Anal Chem 81:6750-5. 2009
    ..In conjunction with a potent new matrix, oxamide, requiring smaller laser fluence, distinct features of the 6 microm IR wavelength range are anticipated to remove one of the limitations of MALDI MS for biomolecular analysis...
  32. ncbi request reprint Expression of vascular endothelial growth factor, placental growth factor, and their receptors Flt-1 and KDR in human placenta under pathologic conditions
    Kaori Kumazaki
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, Japan
    Hum Pathol 33:1069-77. 2002
    ..Thus, various functions of VEGF family members participate in the development of pathologic changes in the placenta...
  33. ncbi request reprint Detection of cytomegalovirus DNA in human placenta
    Kaori Kumazaki
    Department of Pathology and Laboratory Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    J Med Virol 68:363-9. 2002
    ..These results suggest that the CMV genome is common in placentas at later gestational ages and in those of earlier gestational ages with certain maternal complications...
  34. ncbi request reprint Decomposition of protein nitrosothiolsin matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry
    Rina Kaneko
    Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka 594 1101, Japan
    J Mass Spectrom 38:526-30. 2003
    ..With MALDI, the S-NO bond cleavage occurred during the ionization process, and the subsequent reduction generated [M + H - 29](+) ions...
  35. ncbi request reprint Placental features in preterm infants with periventricular leukomalacia
    Kaori Kumazaki
    Department of Pathology, Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Pediatrics 109:650-5. 2002
    ..We investigated the relationship between placental findings and periventricular leukomalacia (PVL) to obtain a better understanding of its cause...
  36. ncbi request reprint Derivatization for stabilizing sialic acids in MALDI-MS
    Sadanori Sekiya
    Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, Nakagyo ku, Kyoto 604 8511, Japan
    Anal Chem 77:4962-8. 2005
    ..In addition, amidation with (15)N-labeled ammonium chloride decreases the mass shift from the acid to amide form to just 0.013, reducing the complexity of mass spectral interpretation and database searching...
  37. ncbi request reprint Differential analysis of site-specific glycans on plasma and cellular fibronectins: application of a hydrophilic affinity method for glycopeptide enrichment
    Michiko Tajiri
    CREST, Japan Science and Technology Agency, 4 1 8 Honcho Kawaguchi, Saitama 332 0012, Japan
    Glycobiology 15:1332-40. 2005
    ..The results also demonstrated that the enriched glycopeptide provides resources for site-specific analysis of oligosaccharides in glycoproteomics...
  38. ncbi request reprint Tyrosine phosphorylation of clathrin heavy chain under oxidative stress
    Yoshito Ihara
    Department of Biochemistry and Molecular Biology in Disease, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1 12 4 Sakamoto, Japan
    Biochem Biophys Res Commun 297:353-60. 2002
    ..The present study demonstrates that oxidative stress affects intracellular vesicular trafficking via the alteration of clathrin-dependent vesicular trafficking...
  39. ncbi request reprint Characterization of the ligand-binding specificities of integrin alpha3beta1 and alpha6beta1 using a panel of purified laminin isoforms containing distinct alpha chains
    Ryoko Nishiuchi
    Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871
    J Biochem 134:497-504. 2003
    ....
  40. ncbi request reprint Differential expression and localization of decorin in human choriodecidual membrane during preterm and term pregnancy
    Kazuhide Ogita
    Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Suita, Japan
    Am J Reprod Immunol 51:204-10. 2004
    ..This study was aimed to isolate cDNAs for genes whose expression levels are dynamically regulated at the feto-maternal interface around parturition...
  41. ncbi request reprint N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function
    Tomoya Isaji
    Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Miyagi 981 8558, Japan
    J Biol Chem 281:33258-67. 2006
    ....
  42. pmc Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151
    Ryoko Nishiuchi
    Institute for Protein Research and Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 102:1939-44. 2005
    ....
  43. pmc Myosin motor Myo1c and its receptor NEMO/IKK-gamma promote TNF-alpha-induced serine307 phosphorylation of IRS-1
    Yoshitaka Nakamori
    Division of Molecular Analysis of Human Disorders, Department of Bio Signal Analysis, Yamaguchi University Graduate School of Medicine, Ube 755 8505, Japan
    J Cell Biol 173:665-71. 2006
    ..Thus, motor protein Myo1c and its receptor protein NEMO act cooperatively to form the IKK-IRS-1 complex and function in TNF-alpha-induced insulin resistance...
  44. ncbi request reprint Effect of reversed heme orientation on circular dichroism and cooperative oxygen binding of human adult hemoglobin
    Masako Nagai
    Research Center for Micro Nano Technology, Hosei University, Tokyo 184 0003, Japan masako nagai gv k hosei ac jp
    Biochemistry 47:517-25. 2008
    ..These results indicate that changes in the heme-globin contact exert a discernible influence on CD spectra and cooperative oxygen binding...