Affiliation: Osaka Prefecture University
Ueda M, Asano T, Nakazawa M, Miyatake K, Inouye K. Purification and characterization of novel raw-starch-digesting and cold-adapted alpha-amylases from Eisenia foetida. Comp Biochem Physiol B Biochem Mol Biol. 2008;150:125-30 pubmed publisher
..0-9.0 and 50-60 degrees C. Both Amy I and II exhibited activities at 10 degrees C. The enzymes were inhibited by metal ions Cu(2+), Fe(2+), and Hg(2+), and hydrolyzed raw starch into glucose, maltose and maltotriose as end products. ..
Ueda M, Hirano Y, Fukuhara H, Naka Y, Nakazawa M, Sakamoto T, et al
. Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida. Enzyme Microb Technol. 2018;117:15-22 pubmed publisher
..X-ray crystallographic analysis supports the hydrolysis and transglycosylation reaction mechanism determined by HPLC and HPAEC analyses. ..
Ueda M, Maruyama T, Kawasaki K, Nakazawa M, Sakaguchi M. Purification, Characterization, and Gene Cloning of a Cold-Adapted Endo-1,4-Î²-glucanase from Bellamya chinensis laeta. Mol Biotechnol. 2016;58:241-50 pubmed publisher
Matsumoto S, Yamada H, Kunishige Y, Takenaka S, Nakazawa M, Ueda M, et al
. Identification of a novel Penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene. Enzyme Microb Technol. 2017;98:76-85 pubmed publisher
..To our knowledge, this is the first report of a gene encoding a RG rhamnohydrolase. ..
Ueda M, Shioyama T, Nakadoi K, Nakazawa M, Sakamoto T, Iwamoto T, et al
. Cloning and expression of a chitinase gene from Eisenia fetida. Int J Biol Macromol. 2017;104:1648-1655 pubmed publisher
..The optimal catalytic temperature of rEF-Chi was identified as 60Â°C, and the hydrolytic product from colloidal chitin was N-acetyl-chitobiose, suggesting that EF-Chi is an exo-type enzyme. ..
Ueda M, Kubo T, Miyatake K, Nakamura T. Purification and characterization of fibrinolytic alkaline protease from Fusarium sp. BLB. Appl Microbiol Biotechnol. 2007;74:331-8 pubmed
..The N-terminal amino acid sequence of the enzyme showed a similarity to those of proteases from Fusarium sp., Streptomyces griseus, Bos taurus bovine, Katsuwo pelamis digestive tract, and Lumbricus rubellus. ..
Ueda M, Ohata K, Konishi T, Sutrisno A, Okada H, Nakazawa M, et al
. A novel goose-type lysozyme gene with chitinolytic activity from the moderately thermophilic bacterium Ralstonia sp. A-471: cloning, sequencing, and expression. Appl Microbiol Biotechnol. 2009;81:1077-85 pubmed publisher
..Thus, Ra-ChiC was found to be a novel enzyme in its structural and functional properties...
Ueda M, Goto T, Nakazawa M, Miyatake K, Sakaguchi M, Inouye K. A novel cold-adapted cellulase complex from Eisenia foetida: characterization of a multienzyme complex with carboxymethylcellulase, beta-glucosidase, beta-1,3 glucanase, and beta-xylosidase. Comp Biochem Physiol B Biochem Mol Biol. 2010;157:26-32 pubmed publisher
..The CMCase activity in the purified enzyme complex at 15 degrees C was 44% of that obtained at the optimal temperature. The optimum pH of the EF-CMCase25 in the purified enzyme complex was pH 5.0 and stable at pH 7.0-9.0. ..
Maruta A, Yamane M, Matsubara M, Suzuki S, Nakazawa M, Ueda M, et al
. A novel Î±-galactosidase from Fusarium oxysporum and its application in determining the structure of the gum arabic side chain. Enzyme Microb Technol. 2017;103:25-33 pubmed publisher
..The novel FoGP1 might be used to modify the physical properties of gum arabic, which is an industrially important polysaccharide used as an emulsion stabilizer and coating agent. ..