Naoyuki Taniguchi

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint Frontiers in glycomics; bioinformatics and biomarkers in disease. September 11-13, 2006 Natcher Conference Center, NIH Campus, Bethesda, MD, USA
    Naoyuki Taniguchi
    Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Proteomics 7:1360-3. 2007
  2. doi request reprint Glycomic analyses of glycoproteins in bile and serum during rat hepatocarcinogenesis
    Tsutomu Nakagawa
    Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    J Proteome Res 9:4888-96. 2010
  3. ncbi request reprint Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8
    Hideyuki Ihara
    Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Taniguchi Research Group, 4th Floor, Center for Advanced Science and Innovation, Osaka University, 2 1, Yamadaoka Suita, Osaka 565 0871, Japan
    Glycobiology 17:455-66. 2007
  4. doi request reprint Branched N-glycans regulate the biological functions of integrins and cadherins
    Yanyang Zhao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    FEBS J 275:1939-48. 2008
  5. doi request reprint Functional roles of N-glycans in cell signaling and cell adhesion in cancer
    Yan Yang Zhao
    Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Cancer Sci 99:1304-10. 2008
  6. ncbi request reprint Involvement of oligosaccharide changes in alpha5beta1 integrin in a cisplatin-resistant human squamous cell carcinoma cell line
    Susumu Nakahara
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Mol Cancer Ther 2:1207-14. 2003
  7. ncbi request reprint From glycomics to functional glycomics of sugar chains: Identification of target proteins with functional changes using gene targeting mice and knock down cells of FUT8 as examples
    Akihiro Kondo
    Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Biochim Biophys Acta 1764:1881-9. 2006
  8. ncbi request reprint Beta1,4-N-Acetylglucosaminyltransferase III down-regulates neurite outgrowth induced by costimulation of epidermal growth factor and integrins through the Ras/ERK signaling pathway in PC12 cells
    Jianguo Gu
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka Suita, Osaka 565 0871, Japan
    Glycobiology 14:177-86. 2004
  9. ncbi request reprint Fucosylation of N-glycans regulates the secretion of hepatic glycoproteins into bile ducts
    Tsutomu Nakagawa
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    J Biol Chem 281:29797-806. 2006
  10. ncbi request reprint [Importance of sugar chain functions and their characterization]
    Naoyuki Taniguchi
    Tanpakushitsu Kakusan Koso 48:901-9. 2003

Detail Information

Publications86

  1. ncbi request reprint Frontiers in glycomics; bioinformatics and biomarkers in disease. September 11-13, 2006 Natcher Conference Center, NIH Campus, Bethesda, MD, USA
    Naoyuki Taniguchi
    Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Proteomics 7:1360-3. 2007
    ....
  2. doi request reprint Glycomic analyses of glycoproteins in bile and serum during rat hepatocarcinogenesis
    Tsutomu Nakagawa
    Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    J Proteome Res 9:4888-96. 2010
    ..These results suggested that the sorting machinery through fucosylation might be disrupted in the liver with HCC...
  3. ncbi request reprint Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8
    Hideyuki Ihara
    Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Taniguchi Research Group, 4th Floor, Center for Advanced Science and Innovation, Osaka University, 2 1, Yamadaoka Suita, Osaka 565 0871, Japan
    Glycobiology 17:455-66. 2007
    ....
  4. doi request reprint Branched N-glycans regulate the biological functions of integrins and cadherins
    Yanyang Zhao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    FEBS J 275:1939-48. 2008
    ..Collectively, N-glycans on adhesion molecules, especially those on E-cadherin and integrins, play key roles in cell-cell and cell-extracellular matrix interactions, thereby affecting cancer metastasis...
  5. doi request reprint Functional roles of N-glycans in cell signaling and cell adhesion in cancer
    Yan Yang Zhao
    Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Cancer Sci 99:1304-10. 2008
    ..We also suggest that the modulation of N-glycans of those receptors alters their important functions such as cell signaling and cell adhesion which are implicated in cancer invasion and metastasis...
  6. ncbi request reprint Involvement of oligosaccharide changes in alpha5beta1 integrin in a cisplatin-resistant human squamous cell carcinoma cell line
    Susumu Nakahara
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Mol Cancer Ther 2:1207-14. 2003
    ..These results suggest that modification of oligosaccharides of alpha5beta1 integrin represents one of the possible mechanisms of drug resistance in head and neck cancer cells...
  7. ncbi request reprint From glycomics to functional glycomics of sugar chains: Identification of target proteins with functional changes using gene targeting mice and knock down cells of FUT8 as examples
    Akihiro Kondo
    Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Biochim Biophys Acta 1764:1881-9. 2006
    ..This approach is based on glycan profiling by CE/MS and LC/MS followed by proteomic approaches, including 2-DE/1-DE and lectin blot techniques and identification of functional changes of sugar chains...
  8. ncbi request reprint Beta1,4-N-Acetylglucosaminyltransferase III down-regulates neurite outgrowth induced by costimulation of epidermal growth factor and integrins through the Ras/ERK signaling pathway in PC12 cells
    Jianguo Gu
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka Suita, Osaka 565 0871, Japan
    Glycobiology 14:177-86. 2004
    ....
  9. ncbi request reprint Fucosylation of N-glycans regulates the secretion of hepatic glycoproteins into bile ducts
    Tsutomu Nakagawa
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    J Biol Chem 281:29797-806. 2006
    ..A disruption in this system might involve an increase in fucosylated AFP in the serum of patients with HCC...
  10. ncbi request reprint [Importance of sugar chain functions and their characterization]
    Naoyuki Taniguchi
    Tanpakushitsu Kakusan Koso 48:901-9. 2003
  11. ncbi request reprint [Regulation of growth and differentiation by oligosaccharides]
    Eiji Miyoshi
    Tanpakushitsu Kakusan Koso 48:929-33. 2003
  12. pmc Man2C1, an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol
    Tadashi Suzuki
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, 565 0871, Japan
    Biochem J 400:33-41. 2006
    ....
  13. ncbi request reprint Site-specific labeling of cytoplasmic peptide:N-glycanase by N,N'-diacetylchitobiose-related compounds
    Tadashi Suzuki
    21st Center of Excellence Program, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    J Biol Chem 281:22152-60. 2006
    ..These results suggest that cytoplasmic PNGase has a separate binding site for chitobiose and other carbohydrates, and haloacetamide derivatives can irreversibly inhibit that catalytic Cys in a highly specific manner...
  14. ncbi request reprint [Functions of Gal 3-sulfotransferases]
    Kohichi Honke
    Tanpakushitsu Kakusan Koso 48:963-6. 2003
  15. ncbi request reprint Dual enzymatic properties of the cytoplasmic peptide: N-glycanase in C. elegans
    Tadashi Suzuki
    Department of Biochemistry, Osaka University Graduate School of Medicine, Japan
    Biochem Biophys Res Commun 358:837-41. 2007
    ..Occurrence of a C. elegans mutation affecting a catalytic residue in the PNGase domain strongly suggests the functional importance of this protein in higher eukaryotes...
  16. ncbi request reprint Sulfotransferases and sulfated oligosaccharides
    Koichi Honke
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Med Res Rev 22:637-54. 2002
    ..Investigations on the relationship of sulfated oligosaccharides to human diseases including hereditary deficiency, cancer, inflammation, and infection may provide hints for curing disastrous diseases...
  17. ncbi request reprint A common pathway for intracellular reactive oxygen species production by glycoxidative and nitroxidative stress in vascular endothelial cells and smooth muscle cells
    Naoyuki Taniguchi
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Ann N Y Acad Sci 1043:521-8. 2005
    ..The elucidation of the pathway for extracellular stress-induced reactive oxygen species (ROS) production would be important for our understanding of the role of ROS as signaling molecules...
  18. doi request reprint Cytoplasmic and serum galectin-3 in diagnosis of thyroid malignancies
    Hidenori Inohara
    Department of Otolaryngology, Osaka University School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 376:605-10. 2008
    ..Accordingly, relative galectin-3 value allows a definitive diagnosis of papillary carcinoma independent of cellular morphology, whereas serum galectin-3 does not serve as a marker for papillary carcinoma...
  19. ncbi request reprint Significance of the association between heparin-binding epidermal growth factor-like growth factor and CD9 in human gastric cancer
    Yoko Murayama
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka, Japan
    Int J Cancer 98:505-13. 2002
    ..These results indicate that the coexpression of proHB-EGF and CD9 may be involved in the tumorigenesis and/or proliferation of gastric cancers in a juxtacrine manner...
  20. pmc Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Mol Cell Proteomics 9:719-27. 2010
    ..In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful...
  21. ncbi request reprint Regulation of integrin functions by N-glycans
    Jianguo Gu
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Glycoconj J 21:9-15. 2004
    ..This review summarizes effects of the posttranslational modification of N-glycans of alpha3beta1 and alpha5beta1 integrins on their association, activation and biological functions, by using biochemical and genetic approaches...
  22. ncbi request reprint Acrolein induces Hsp72 via both PKCdelta/JNK and calcium signaling pathways in human umbilical vein endothelial cells
    Yoshiko Misonou
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Free Radic Res 39:507-12. 2005
    ..The results suggest that HUVEC have a unique defense system against cell damage by acrolein in which Hsp72 is induced via activation of both the PKCd/JNK and the calcium pathway...
  23. ncbi request reprint Relationship between elevated FX expression and increased production of GDP-L-fucose, a common donor substrate for fucosylation in human hepatocellular carcinoma and hepatoma cell lines
    Katsuhisa Noda
    Department of Biochemistry, Division of Molecular Therapy Science, Osaka University, Graduate School of Medicine, 1 7 Yamada oka, Suita, Osaka 565 0871, Japan
    Cancer Res 63:6282-9. 2003
    ..Thus, an elevation in GDP-L-fucose levels and the up-regulation of FX expression represent potential markers for HCC...
  24. ncbi request reprint Decoding sugar functions by identifying target glycoproteins
    Naoyuki Taniguchi
    Department of Disease Glycomics, Institute for Microbial Diseases, Osaka University, 2 1 Suita, Osaka 565 0871, Japan
    Curr Opin Struct Biol 16:561-6. 2006
    ..Functional glycomics and the identification of target proteins will provide a new way to elucidate the nature of disease in the post-genomic era...
  25. pmc Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice
    Shinji Takamatsu
    Department of Disease Glycomics, The Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, 567 0041, Japan
    Glycobiology 20:485-97. 2010
    ..This is the first report that mammalian organs have highly organized glycomic compensation systems to preserve N-glycan branch complexity...
  26. doi request reprint Simultaneous determination of nucleotide sugars with ion-pair reversed-phase HPLC
    Kazuki Nakajima
    Disease Glycomics, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
    Glycobiology 20:865-71. 2010
    ....
  27. ncbi request reprint Fucosylated haptoglobin is a novel marker for pancreatic cancer: a detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation
    Noriko Okuyama
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Int J Cancer 118:2803-8. 2006
    ..One is that pancreatic cancer cells, themselves, produce fucosylated haptoglobin; the other is that pancreatic cancer produces a factor, which induces the production of fucosylated haptoglobin in the liver...
  28. ncbi request reprint Down-regulation of trypsinogen expression is associated with growth retardation in alpha1,6-fucosyltransferase-deficient mice: attenuation of proteinase-activated receptor 2 activity
    Wenzhe Li
    Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka, Japan
    Glycobiology 16:1007-19. 2006
    ....
  29. ncbi request reprint A catalytically inactive beta 1,4-N-acetylglucosaminyltransferase III (GnT-III) behaves as a dominant negative GnT-III inhibitor
    Hideyuki Ihara
    Department of Biochemistry, Osaka University Medical School, Suita, Osaka, Japan
    Eur J Biochem 269:193-201. 2002
    ..This type of 'dominant negative glycosyltransferase', identified has potential value as a powerful tool for defining the precise biological roles of the bisecting GlcNAc structure...
  30. doi request reprint The implication of N-acetylglucosaminyltransferase V expression in gastric cancer
    Hai Tian
    Department of Molecular Pathology, Graduate School of Medicine and Health Science, Osaka University, Suita, Osaka, Japan
    Pathobiology 75:288-94. 2008
    ..GnT-V expression was studied immunohistochemically in gastric cancer to compare clinicopathological parameters and evaluate the role of GnT-V in the prognosis of gastric cancer...
  31. ncbi request reprint Bisecting GlcNAc mediates the binding of annexin V to Hsp47
    Cong Xiao Gao
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Glycobiology 15:1067-75. 2005
    ..Collectively, these results suggest that the binding of annexin V to Hsp47 is mediated by a bisecting GlcNAc oligosaccharide structure and that Hsp47 is an intracellular ligand glycoprotein for annexin V...
  32. ncbi request reprint Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change
    Rina Takamiya
    Department of Biochemistry, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Am J Physiol Cell Physiol 288:C253-9. 2005
    ..These results suggest that mutated Cu,Zn-SOD in FALS causes cytoskeletal changes by associating with actin, which subsequently causes G2/M arrest and growth retardation...
  33. ncbi request reprint Liver regeneration in heparin-binding EGF-like growth factor transgenic mice after partial hepatectomy
    Shinichi Kiso
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Gastroenterology 124:701-7. 2003
    ..This study describes the effects of HB-EGF on liver regeneration after 70% partial hepatectomy in proHB-EGF transgenic mice with liver-specific expression. Methods &..
  34. ncbi request reprint A glycomic approach to hepatic tumors in N-acetylglucosaminyltransferase III (GnT-III) transgenic mice induced by diethylnitrosamine (DEN): identification of haptoglobin as a target molecule of GnT-III
    Atsuko Ekuni
    Department of Biochemistry, Osaka University, Graduate School of Medicine, Osaka, Japan
    Free Radic Res 36:827-33. 2002
    ..This paper is the first report that shows a relationship between the sugar chains of glycoproteins with radical scavenger activity and hepatocarcinogenesis...
  35. ncbi request reprint Phenotype changes of Fut8 knockout mouse: core fucosylation is crucial for the function of growth factor receptor(s)
    Xiangchun Wang
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Methods Enzymol 417:11-22. 2006
    ..Collectively, these results suggest that core fucosylation is crucial for growth factor receptors such as TGF-beta1 and EGF receptor-mediated biological functions...
  36. ncbi request reprint A high expression of GDP-fucose transporter in hepatocellular carcinoma is a key factor for increases in fucosylation
    Kenta Moriwaki
    Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Glycobiology 17:1311-20. 2007
    ..These findings suggest that the upregulation of GDP-Fuc Tr plays a pivotal role in increased fucosylation in HCC and represents an attractive target for new treatments and diagnosis for HCC...
  37. doi request reprint Site-specific analysis of N-glycans on haptoglobin in sera of patients with pancreatic cancer: a novel approach for the development of tumor markers
    Miyako Nakano
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka, Japan
    Int J Cancer 122:2301-9. 2008
    ..Thus, the present study provides evidence that site-specific analyses of N-glycans may be useful as novel tumor markers for PC...
  38. ncbi request reprint Human disease glycomics/proteome initiative (HGPI)
    Naoyuki Taniguchi
    Department of Disease Glycomics, Institute for Microbial Diseases, Osaka University, Suita, Osaka 567 0871, Japan
    Mol Cell Proteomics 7:626-7. 2008
  39. doi request reprint Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells
    Daisuke Osumi
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka, Japan
    Cancer Sci 100:888-95. 2009
    ..Thus, it was suggested that core fucosylation regulates the processing of oligosaccharides and turnover of E-cadherin. These results suggest a possible role of core fucosylation in the regulation of cell-cell adhesion in cancer...
  40. ncbi request reprint Oxidative stress caused by inactivation of glutathione peroxidase and adaptive responses
    Yasuhide Miyamoto
    Department of Biochemistry, Osaka University Medical School, Osaka, 565 0871, Japan
    Biol Chem 384:567-74. 2003
    ....
  41. ncbi request reprint Apolipoprotein E activates Akt pathway in neuro-2a in an isoform-specific manner
    Isabelle Laffont
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Biochem Biophys Res Commun 292:83-7. 2002
    ..Our results suggest that apoE might affect cell metabolism and survival in neurons in an isoform-specific manner by inducing novel signaling pathways...
  42. doi request reprint Deficiency of GMDS leads to escape from NK cell-mediated tumor surveillance through modulation of TRAIL signaling
    Kenta Moriwaki
    Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine, Osaka, Japan
    Gastroenterology 137:188-98, 198.e1-2. 2009
    ..The mutations resulted in resistance to TRAIL-induced apoptosis followed by escape from immune surveillance...
  43. ncbi request reprint From glycobiology to systems glycobiology: international network with Japanese scientists through consortia
    Naoyuki Taniguchi
    Department of Biochemistry, Osaka University, Graduate School of Medicine, Osaka, Japan
    IUBMB Life 58:269-72. 2006
  44. doi request reprint Requirement of Fut8 for the expression of vascular endothelial growth factor receptor-2: a new mechanism for the emphysema-like changes observed in Fut8-deficient mice
    Xiangchun Wang
    Department of Disease Glycomics, Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Biochem 145:643-51. 2009
    ..It is well known that, in emphysema, ceramide expression can be greatly enhanced by blockade of the VEGFR-2. Thus, suppression of VEGFR-2 expression may provide a novel explanation for the emphysema-like changes in Fut8(-/-) mice...
  45. ncbi request reprint NF-kappaB activation in non-parenchymal liver cells after partial hepatectomy in rats: possible involvement in expression of heparin-binding epidermal growth factor-like growth factor
    Shigeru Sakuda
    Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, 565 0871 Osaka, Japan
    J Hepatol 36:527-33. 2002
    ..The aims of this study were to identify NF-B-activated cells and to clarify their involvement in HB-EGF expression after PH...
  46. ncbi request reprint Acrolein induces cyclooxygenase-2 and prostaglandin production in human umbilical vein endothelial cells: roles of p38 MAP kinase
    Yong Seek Park
    Department of Biochemistry, Research Institute for Microbial Diseases, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Arterioscler Thromb Vasc Biol 27:1319-25. 2007
    ..Acrolein, a known toxin in tobacco smoke, might be involved in atherogenesis. This study examined the effect of acrolein on expression of cyclooxygenase-2 (COX-2) and prostaglandin (PG) production in endothelial cells...
  47. ncbi request reprint Differential expression of glutathione reductase and cytosolic glutathione peroxidase, GPX1, in developing rat lungs and kidneys
    Tsuneko Fujii
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Free Radic Res 36:1041-9. 2002
    ..Since many gene products show GSH-dependent preoxidase activity, other peroxidase(s) may be induced to compensate for the low GPX1 levels at stages with high GR expression...
  48. ncbi request reprint Introduction of bisecting GlcNAc in N-glycans of adenylyl cyclase III enhances its activity
    Wei Li
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Glycobiology 17:655-62. 2007
    ..Thus, the structure of N-glycans of ACIII regulates its enzymatic activity and downstream signaling...
  49. ncbi request reprint Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Glycobiology 17:411-22. 2007
    ....
  50. ncbi request reprint Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching
    Shinji Ihara
    Department of Biochemistry, Osaka University Medical School graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:16960-7. 2002
    ..These results indicate that this process is a key element in malignant transformation, as the direct result of oligosaccharide modification...
  51. ncbi request reprint A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity
    Takashi Saito
    Department of Biochemistry, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    J Biol Chem 277:17002-8. 2002
    ..These findings provide some novel information on the relationship between GnT-V and tumor metastasis. The inhibition of GnT-V secretion or its expression represents a novel potential strategy for the inhibition of tumor angiogenesis...
  52. ncbi request reprint The action of N-acetylglucosaminyltransferase-V is prevented by the bisecting GlcNAc residue at the catalytic step
    Ken Sasai
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Japan
    FEBS Lett 522:151-5. 2002
    ..It was also found that the presence of the 2-N-acetyl group in the bisecting monosaccharide moiety plays a critical role in determining the catalytic efficiency of the enzyme...
  53. ncbi request reprint Roles of cell adhesion molecules in tumor angiogenesis induced by cotransplantation of cancer and endothelial cells to nude rats
    Kou Tei
    Department of Molecular Pathology, Research Institute, Aichi Cancer Center, Nagoya 464 8681, Japan
    Cancer Res 62:6289-96. 2002
    ....
  54. ncbi request reprint A sugar-coated switch for cellular growth and arrest
    Naoyuki Taniguchi
    Nat Chem Biol 3:307-9. 2007
  55. ncbi request reprint Functional proteomics study reveals that N-Acetylglucosaminyltransferase V reinforces the invasive/metastatic potential of colon cancer through aberrant glycosylation on tissue inhibitor of metalloproteinase-1
    Yong Sam Kim
    Daejeon KRIBB Fred Hutchinson Cancer Research Center Research Cooperation Center, Daejeon, Korea
    Mol Cell Proteomics 7:1-14. 2008
    ..Integrated data, both of TIMP-1 expression level and aberrant glycosylation, could provide important information to aid to improve the clinical outcome of colon cancer patients...
  56. doi request reprint Glycomic analysis of alpha-fetoprotein L3 in hepatoma cell lines and hepatocellular carcinoma patients
    Takatoshi Nakagawa
    Department of Glycotherapeutics, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Osaka 565 0871, Japan
    J Proteome Res 7:2222-33. 2008
    ..Moreover, we identified the N-glycan structures of AFP in HCC patients...
  57. doi request reprint Dual-gradient high-performance liquid chromatography for identification of cytosolic high-mannose-type free glycans
    Tadashi Suzuki
    RIKEN Institute of Physical and Chemical Research, Wako, Saitama 351 0198, Japan
    Anal Biochem 381:224-32. 2008
    ..This HPLC, therefore, is a powerful method for identification of the structures of PA-labeled glycans, especially Gn1-type glycans, isolated from the cytosol of animal cells...
  58. ncbi request reprint The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST)
    Masaru Koma
    Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Yamadaoka, Suita, 565 0871, Japan
    J Biochem 131:517-22. 2002
    ..These data indicate that GP3ST is very effective in reducing xenoepitope levels...
  59. pmc Paranodal junction formation and spermatogenesis require sulfoglycolipids
    Koichi Honke
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 99:4227-32. 2002
    ..These data show a critical role for sulfoglycolipids in myelin function and spermatogenesis...
  60. ncbi request reprint Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose
    Kengo Matsumura
    Research Institute, Gekkeikan Sake Company Ltd, 300 Katahara cho, Fushimi ku, Kyoto 612 8361, Japan
    J Biol Chem 282:15700-8. 2007
    ..Taken together, these results suggest that AOL is more suitable for detecting core fucose than AAL or LCA...
  61. ncbi request reprint Identification of the binding site of methylglyoxal on glutathione peroxidase: methylglyoxal inhibits glutathione peroxidase activity via binding to glutathione binding sites Arg 184 and 185
    Yong Seek Park
    Department of Biochemistry, Osaka University Medical School, Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Free Radic Res 37:205-11. 2003
    ..These data suggest that dicarbonyl compounds are able to directly inactivate GPx, resulting in an increase in intracellular peroxides which are responsible for oxidative cellular damage...
  62. ncbi request reprint Core fucosylation regulates epidermal growth factor receptor-mediated intracellular signaling
    Xiangchun Wang
    Department of Biochemistry, Osaka University Graduate School of Medicine, Japan
    J Biol Chem 281:2572-7. 2006
    ..Collectively, these results strongly suggest that core fucosylation is essential for EGF receptor-mediated biological functions...
  63. ncbi request reprint Expression of N-acetylglucosaminyltransferase V in the development of human esophageal cancers: immunohistochemical data from carcinomas and nearby noncancerous lesions
    Yoko Ishibashi
    Department of Medical Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Oncology 69:301-10. 2005
    ..However, neither GnT-V expression nor its clinical significance has been previously examined in human normal, premalignant and malignant esophageal tissues...
  64. ncbi request reprint Differences in recognition of sulfated and sialylated carbohydrate chains
    Koichi Honke
    Department of Biochemistry, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    Methods Enzymol 363:222-9. 2003
  65. ncbi request reprint Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration
    Tomoya Isaji
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 279:19747-54. 2004
    ..These findings suggest that the modification of N-glycan of integrin by GnT-III inhibits its ligand binding ability, subsequently leading to the down-regulation of integrin-mediated signaling...
  66. ncbi request reprint Lactosylsulfatide expression in hepatocellular carcinoma cells enhances cell adhesion to vitronectin and intrahepatic metastasis in nude mice
    Xing Zhong Wu
    Department of Biochemistry, Shanghai Medical School, Fudan University, Shanghai, People s Republic of China
    Int J Cancer 110:504-10. 2004
    ..Furthermore, SM3 expression promoted intrahepatic metastasis in nude mice. Thus, SM3 may play an important role in the metastasis of HCC cells by causing the interaction of integrin alphaVbeta3 with vitronectin...
  67. ncbi request reprint Production of a recombinant single-chain variable-fragment (scFv) antibody against sulfoglycolipid
    Xinyao Cheng
    Department of Biochemistry, Osaka University Medical School, Osaka 565 0871
    J Biochem 137:415-21. 2005
    ..The scFv antibody that was displayed at the tip of the M13 phage in the form of a g3p fusion protein bound to sulfatide. Furthermore, a soluble form of the scFv antibody was also found to bind to the sulfoglycolipids in ELISA...
  68. ncbi request reprint [From proteomics to functional glycomics: importance of functional analysis of sugar chains]
    Eiji Miyoshi
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2, Yamada oka, Suita 565 0871, Japan
    Seikagaku 76:1337-43. 2004
  69. ncbi request reprint [Abnormalities in myelinogenesis in glycolipid sulfotransferase-KO mice]
    Koichi Honke
    Tanpakushitsu Kakusan Koso 49:2445-50. 2004
  70. ncbi request reprint Different immunoreactivity against monoclonal antibodies between wild-type and mutant copper/zinc superoxide dismutase linked to amyotrophic lateral sclerosis
    Noriko Fujiwara
    Department of Biochemistry, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo 663 8501, Japan
    J Biol Chem 280:5061-70. 2005
    ..The study on the conformational changes in local areas monitoring with mAbs may provide a new insight into the etiology of FALS...
  71. ncbi request reprint Biological roles of sulfoglycolipids and pathophysiology of their deficiency
    Koichi Honke
    Department of Molecular Genetics, Kochi University Medical School, Kohasu, Oko cho, Nankoku, Kochi 783 8505, Japan
    Glycoconj J 21:59-62. 2004
    ..Studies on the molecular mechanisms by which sulfoglycolipids participate in these biological processes are ongoing...
  72. ncbi request reprint HB-EGF is a potent inducer of tumor growth and angiogenesis
    Pat P Ongusaha
    Cancer Biology Program, Hematology and Oncology Division, Beth Israel Deaconess Medical Center and Harvard Medical School, 4 Blackfan Circle, Boston, MA 02115, USA
    Cancer Res 64:5283-90. 2004
    ..These findings establish s-HB-EGF as a potent inducer of tumor growth and angiogenesis and suggest that therapeutic intervention aimed at the inhibition of s-HB-EGF functions may be useful in cancer treatment...
  73. ncbi request reprint Carbohydrate binding activity of annexin V toward a bisecting N-acetylglucosamine
    Cong Xiao Gao
    Department of Biochemistry, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    Methods Enzymol 363:34-47. 2003
  74. ncbi request reprint Reaction mechanism and substrate specificity for nucleotide sugar of mammalian alpha1,6-fucosyltransferase--a large-scale preparation and characterization of recombinant human FUT8
    Hideyuki Ihara
    Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Glycobiology 16:333-42. 2006
    ..These results may also be applicable to other fucosyltransferases and glycosyltransferases...
  75. ncbi request reprint Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin
    Shinji Ihara
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2, Yamadaoka, Suita, Osaka 565 0871, Japan
    Glycobiology 14:139-46. 2004
    ..This is the first example of a demonstration of a direct relationship between beta1-6 GlcNAc branching and a biological function at the protein level...
  76. ncbi request reprint Deletion of core fucosylation on alpha3beta1 integrin down-regulates its functions
    Yanyang Zhao
    Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:38343-50. 2006
    ..The reintroduction of Fut8 potentially restored laminin 5-induced migration and intracellular signaling. Collectively, these results suggested that core fucosylation is essential for the functions of alpha3beta1 integrin...
  77. ncbi request reprint Caveolin-1 regulates the functional localization of N-acetylglucosaminyltransferase III within the golgi apparatus
    Ken Sasai
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 278:25295-301. 2003
    ..Caveolin-1 may modify the biosynthetic pathway of sugar chains via the regulation of the intra-Golgi subcompartment localization of this key glycosyltransferase...
  78. ncbi request reprint N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function
    Tomoya Isaji
    Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai, Miyagi 981 8558, Japan
    J Biol Chem 281:33258-67. 2006
    ....
  79. ncbi request reprint N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha3beta1 integrin-mediated cell migration
    Yanyang Zhao
    Departments of Biochemistry and Glycotherapeutics, Osaka University Graduate School of Medicine, B1, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:32122-30. 2006
    ..Taken together, our results demonstrate for the first time that GnT-III and GnT-V can competitively modify the same target glycoprotein and furthermore positively or negatively regulate its biological functions...
  80. ncbi request reprint An enzymatic method of analysis for GDP-L-fucose in biological samples, involving high-performance liquid chromatography
    Katsuhisa Noda
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Anal Biochem 310:100-6. 2002
    ..This assay is applicable to the determination of the GDP-L-fucose content in various cancer cell lines as well as rat liver and would be useful in developing a better understanding of the fucosylation potential of such cells and tissues...
  81. ncbi request reprint Molecular cloning and characterization of human GnT-IX, a novel beta1,6-N-acetylglucosaminyltransferase that is specifically expressed in the brain
    Kei Ichiro Inamori
    Department of Biochemistry, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 278:43102-9. 2003
    ..These results suggest that GnT-IX is responsible for the synthesis of a unique oligosaccharide structure in the brain...
  82. ncbi request reprint Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion
    Junko Iijima
    Department of Biochemistry, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:13038-46. 2006
    ....
  83. ncbi request reprint beta1,4-N-Acetylglucosaminyltransferase III potentiates beta1 integrin-mediated neuritogenesis induced by serum deprivation in Neuro2a cells
    Masaki Shigeta
    Department of Biochemistry, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Glycobiology 16:564-71. 2006
    ..These data suggest that N-glycans with a bisecting GlcNAc on target molecules, such as beta1 integrin, play important roles in the regulation of neuritogenesis...
  84. ncbi request reprint Demonstration of the expression and the enzymatic activity of N-acetylglucosaminyltransferase IX in the mouse brain
    Kei Ichiro Inamori
    Department of Biochemistry, Osaka University Graduate School of Medicine, Suita, Japan
    Biochim Biophys Acta 1760:678-84. 2006
    ..These results demonstrate, for the first time, that GnT-IX protein is actually expressed and may function as a glycosyltransferase in the brain...
  85. ncbi request reprint Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration
    Yong Sam Kim
    Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea
    Proteomics 6:1187-91. 2006
    ..The enhanced cleavage of PTPkappa in GnT-V-overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration...
  86. ncbi request reprint Attachment of human colon cancer cells to vascular endothelium is enhanced by N-acetylglucosaminyltransferase V
    Kohei Murata
    Department of Surgery, Osaka Medical Center for Cancer and CVD, Nakamichi, Higashinari, Japan
    Oncology 66:492-501. 2004
    ..Inhibition of GnT-V activity may prevent metastasis in colon cancer patients with high sialyl Lewis x expression...