Yoshimi Takai

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. pmc PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter
    Takeshi Baba
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 170:1113-25. 2005
  2. pmc Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
    Genkichi Izumi
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Cell Biol 166:237-48. 2004
  3. pmc Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth
    Shu Hisata
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 178:843-60. 2007
  4. pmc Regulation of SNAREs by tomosyn and ROCK: implication in extension and retraction of neurites
    Toshiaki Sakisaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, 565 0871, Japan
    J Cell Biol 166:17-25. 2004
  5. pmc Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3
    Tsutomu Fujito
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Biol 171:165-73. 2005
  6. pmc Nectin: an adhesion molecule involved in formation of synapses
    Akira Mizoguchi
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 156:555-65. 2002
  7. pmc Activation of Cdc42 by trans interactions of the cell adhesion molecules nectins through c-Src and Cdc42-GEF FRG
    Tatsuro Fukuhara
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 166:393-405. 2004
  8. pmc Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
    W Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Cell Biol 146:1117-32. 1999
  9. ncbi request reprint The roles of cadherins and nectins in interneuronal synapse formation
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Curr Opin Neurobiol 13:520-6. 2003
  10. ncbi request reprint Small GTP-binding proteins
    Y Takai
    Department of Molecular Biology, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    Physiol Rev 81:153-208. 2001

Collaborators

Detail Information

Publications142 found, 100 shown here

  1. pmc PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter
    Takeshi Baba
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 170:1113-25. 2005
    ..We describe the roles and modes of action of PKA and tomosyn in Ca2+-dependent neurotransmitter release...
  2. pmc Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments
    Genkichi Izumi
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Cell Biol 166:237-48. 2004
    ..These results indicate the important role of the Rac/Cdc42-IQGAP1 system in the dynamic organization and maintenance of the E-cadherin-based AJs...
  3. pmc Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth
    Shu Hisata
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 178:843-60. 2007
    ..Thus, the interaction of PDZ-GEF1 with an internalized neurotrophin receptor transported to late endosomes induces sustained activation of both Rap1 and ERK and neurite outgrowth...
  4. pmc Regulation of SNAREs by tomosyn and ROCK: implication in extension and retraction of neurites
    Toshiaki Sakisaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, 565 0871, Japan
    J Cell Biol 166:17-25. 2004
    ..Thus, tomosyn demarcates the plasma membrane by binding to syntaxin-1 phosphorylated by ROCK, and thereby regulates extension and retraction of neurites...
  5. pmc Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3
    Tsutomu Fujito
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Biol 171:165-73. 2005
    ..These results indicate that the down-regulation of Necl-5 induced by its interaction with nectin-3 upon cell-cell contacts may be at least one mechanism underlying contact inhibition of cell movement and proliferation...
  6. pmc Nectin: an adhesion molecule involved in formation of synapses
    Akira Mizoguchi
    Department of Anatomy and Neurobiology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 156:555-65. 2002
    ..These results indicate an important role of the nectin-afadin system in the formation of synapses...
  7. pmc Activation of Cdc42 by trans interactions of the cell adhesion molecules nectins through c-Src and Cdc42-GEF FRG
    Tatsuro Fukuhara
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Biol 166:393-405. 2004
    ..These results indicate that nectins induce the activation of Cdc42 through c-Src and FRG locally at the nectin-based cell-cell adhesion sites...
  8. pmc Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
    W Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Cell Biol 146:1117-32. 1999
    ....
  9. ncbi request reprint The roles of cadherins and nectins in interneuronal synapse formation
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Curr Opin Neurobiol 13:520-6. 2003
    ..Intercellular adhesion through nectins induces activation of Cdc42 and Rac small G proteins, leading to a reorganization of the actin cytoskeleton, gene expression, and cell polarization...
  10. ncbi request reprint Small GTP-binding proteins
    Y Takai
    Department of Molecular Biology, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    Physiol Rev 81:153-208. 2001
    ..Cascades and cross-talks of small G proteins have also been clarified. In this review, functions of small G proteins and their modes of activation and action are described...
  11. ncbi request reprint Nectins and nectin-like molecules: roles in cell adhesion, migration, and polarization
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Cancer Sci 94:655-67. 2003
    ..In this article, the roles and modes of action of nectins and necls in cell adhesion, migration, and polarization are reviewed...
  12. ncbi request reprint Physical and functional interaction of rabphilin-3A with alpha-actinin
    M Kato
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Osaka, Japan
    J Biol Chem 271:31775-8. 1996
    ..It has been shown that reorganization of actin filaments is also involved in Ca2+-dependent exocytosis. Therefore, rabphilin-3A may serve as a linker for Rab3A and cytoskeleton...
  13. ncbi request reprint Nectin and afadin: novel organizers of intercellular junctions
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Sci 116:17-27. 2003
    ..The adhesion system is furthermore involved in the formation of synapses in neurons and the organization of heterotypic junctions between Sertoli cells and spermatids in the testis...
  14. ncbi request reprint Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes
    Shigeki Kakunaga
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Sci 118:1267-77. 2005
    ....
  15. ncbi request reprint Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the formation of adherens junctions in Madin-Darby canine kidney cells
    Tatsuhiro Sato
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:5288-99. 2006
    ..Thus, nectin regulates the assembly and cell-cell adhesion activity of E-cadherin through afadin, nectin signaling, and p120(ctn) for the formation of AJs in Madin-Darby canine kidney cells...
  16. ncbi request reprint Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouse
    Kyoji Takekuni
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 278:5497-500. 2003
    ..These results indicate that nectin-1 and -3 are involved in the localization of PAR-3 at AJs in the neuroepithelial cells of the embryonic telencephalon...
  17. ncbi request reprint Implications of nectin-like molecule-2/IGSF4/RA175/SgIGSF/TSLC1/SynCAM1 in cell-cell adhesion and transmembrane protein localization in epithelial cells
    Tatsushi Shingai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Biol Chem 278:35421-7. 2003
    ..These results indicate the unique localization of Necl-2 and its possible involvement in localization of a transmembrane protein(s) through Pals2...
  18. ncbi request reprint Nectin-like molecule-5/Tage4 enhances cell migration in an integrin-dependent, Nectin-3-independent manner
    Wataru Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Biol Chem 279:18015-25. 2004
    ..We furthermore show here that enhanced motility and metastasis of V12Ras-NIH3T3 cells are at least partly the result of up-regulated Necl-5...
  19. ncbi request reprint Tage4/Nectin-like molecule-5 heterophilically trans-interacts with cell adhesion molecule Nectin-3 and enhances cell migration
    Wataru Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 278:28167-72. 2003
    ..Thus, Tage4 heterophilically trans-interacts with nectin-3 and regulates cell migration. Tage4 is tentatively re-named here nectin-like molecule-5 (necl-5) on the basis of its function and domain structure similar to those of nectins...
  20. ncbi request reprint Expression patterns of nectins and afadin during epithelial remodeling in the mouse embryo
    Noriko Okabe
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka, Japan
    Dev Dyn 230:174-86. 2004
    ..These results suggest that nectins and afadin are involved in dynamic epithelial remodeling during mouse development...
  21. ncbi request reprint Contacts between the commissural axons and the floor plate cells are mediated by nectins
    Noriko Okabe
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Dev Biol 273:244-56. 2004
    ..These results indicate that the contacts between the commissural axons and the floor plate cells are mediated by the hetero-trans-interaction between nectin-1 and -3 and involved in regulation of the trajectory of the commissural axons...
  22. ncbi request reprint A novel rabconnectin-3-binding protein that directly binds a GDP/GTP exchange protein for Rab3A small G protein implicated in Ca(2+)-dependent exocytosis of neurotransmitter
    Hiroshi Kawabe
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 8:537-46. 2003
    ..We have found that two more proteins are co-immunoprecipitated with Rab3 GEP from the CSV fraction of rat brain. We attempted here to isolate and characterize one of them...
  23. ncbi request reprint Vav2 as a Rac-GDP/GTP exchange factor responsible for the nectin-induced, c-Src- and Cdc42-mediated activation of Rac
    Tomomi Kawakatsu
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Biol Chem 280:4940-7. 2005
    ..Cdc42 activated through c-Src then enhanced the GEF activity of tyrosine-phosphorylated Vav2 on Rac1. These results indicate that Vav2 is a GEF responsible for the nectin-induced, c-Src-, and Cdc42-mediated activation of Rac...
  24. ncbi request reprint Necl-5/poliovirus receptor interacts in cis with integrin alphaVbeta3 and regulates its clustering and focal complex formation
    Yukiko Minami
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Biol Chem 282:18481-96. 2007
    ..Thus, Necl-5 has a critical role in integrin alphavbeta3 clustering and focal complex formation...
  25. ncbi request reprint Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling
    Shigeki Kakunaga
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 279:36419-25. 2004
    ..These results indicate that Necl-5 plays roles not only in cell motility but also in cell proliferation...
  26. ncbi request reprint RA-RhoGAP, Rap-activated Rho GTPase-activating protein implicated in neurite outgrowth through Rho
    Tomohiro Yamada
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 280:33026-34. 2005
    ..Knockdown of RA-RhoGAP reduced the Rap1-induced neurite outgrowth. These results indicate that RA-RhoGAP transduces a signal from Rap1 to Rho and regulates the neurite outgrowth...
  27. ncbi request reprint Involvement of the Ras-Ras-activated Rab5 guanine nucleotide exchange factor RIN2-Rab5 pathway in the hepatocyte growth factor-induced endocytosis of E-cadherin
    Toshihiro Kimura
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Biol Chem 281:10598-609. 2006
    ..These results indicate that RIN2 connects Ras to Rab5 in the HGF-induced endocytosis of E-cadherin...
  28. ncbi request reprint Involvement of LMO7 in the association of two cell-cell adhesion molecules, nectin and E-cadherin, through afadin and alpha-actinin in epithelial cells
    Takako Ooshio
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, 2 2 Yamada Oka Suita, Osaka 565 0871, USA
    J Biol Chem 279:31365-73. 2004
    ..These results indicate that LMO7 is an afadin- and alpha-actinin-binding protein that connects the nectin-afadin and E-cadherin-catenin systems through alpha-actinin...
  29. ncbi request reprint ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions
    Masanori Asada
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 278:4103-11. 2003
    ..ADIP may connect the nectin-afadin and E-cadherin-catenin systems through alpha-actinin, and ADIP may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin...
  30. ncbi request reprint Involvement of nectin in the localization of junctional adhesion molecule at tight junctions
    Atsunori Fukuhara
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Oncogene 21:7642-55. 2002
    ..These results suggest that nectin has a role in the localization of JAM at TJs in the process of the formation of the junctional complex in epithelial cells...
  31. pmc Role of nectin in formation of E-cadherin-based adherens junctions in keratinocytes: analysis with the N-cadherin dominant negative mutant
    Yoshinari Tanaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Mol Biol Cell 14:1597-609. 2003
    ..Thus, nectin plays a role in the formation of E-cadherin-based AJs in keratinocytes...
  32. ncbi request reprint Regulation of E-cadherin endocytosis by nectin through afadin, Rap1, and p120ctn
    Takashi Hoshino
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Biol Chem 280:24095-103. 2005
    ....
  33. ncbi request reprint Interaction of integrin alpha(v)beta3 with nectin. Implication in cross-talk between cell-matrix and cell-cell junctions
    Yasuhisa Sakamoto
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:19631-44. 2006
    ..Thus, integrin alpha(v)beta3 and nectin play pivotal roles in the cross-talk between cell-matrix and cell-cell junctions and the formation of cadherin-based AJs...
  34. ncbi request reprint Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus
    Tomoyuki Honda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Mol Cell Neurosci 31:315-25. 2006
    ..In addition, the abnormal mossy fiber trajectory was observed. These results indicate that nectins are involved in the formation of PAJs, which maintain the proper mossy fiber trajectory...
  35. ncbi request reprint Involvement of the c-Src-Crk-C3G-Rap1 signaling in the nectin-induced activation of Cdc42 and formation of adherens junctions
    Taihei Fukuyama
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 280:815-25. 2005
    ..These results indicate that Rap1 is activated by nectins through the c-Src-Crk-C3G signaling and involved in the nectin-induced, c-Src- and FRG-mediated activation of Cdc42 and formation of AJs...
  36. doi request reprint Involvement of the nectin-afadin complex in PDGF-induced cell survival
    Noriyuki Kanzaki
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka, Japan
    J Cell Sci 121:2008-17. 2008
    ..This complex was associated with PDGF receptor on the plasma membrane at cell-cell adhesion sites. Thus, the nectin-afadin complex is involved in PDGF-induced cell survival, at least through the PI3K-Akt signaling pathway...
  37. ncbi request reprint Interaction and localization of Necl-5 and PDGF receptor beta at the leading edges of moving NIH3T3 cells: Implications for directional cell movement
    Hisayuki Amano
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Genes Cells 13:269-84. 2008
    ..These results indicate a key role of Necl-5 in directional cell movement by physically and functionally interacting with both integrin alpha(v)beta(3) and PDGF receptor...
  38. ncbi request reprint Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampus
    Maiko Inagaki
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Comp Neurol 460:514-24. 2003
    ..These results indicate that ZO-1 colocalizes with nectin and afadin at the PAs and that the nectin-afadin system is involved in the localization of ZO-1...
  39. ncbi request reprint Involvement of nectin in the localization of IQGAP1 at the cell-cell adhesion sites through the actin cytoskeleton in Madin-Darby canine kidney cells
    Tatsuo Katata
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    Oncogene 22:2097-109. 2003
    ..These results indicate that nectin is involved in the localization of IQGAP1 at AJs through the actin cytoskeleton...
  40. pmc A novel role of nectins in inhibition of the E-cadherin-induced activation of Rac and formation of cell-cell adherens junctions
    Takashi Hoshino
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Mol Biol Cell 15:1077-88. 2004
    ..These results indicate a novel role of nectins in regulation of the E-cadherin-induced activation of Rac and formation of cell-cell AJs...
  41. ncbi request reprint Nectin-dependent localization of synaptic scaffolding molecule (S-SCAM) at the puncta adherentia junctions formed between the mossy fibre terminals and the dendrites of pyramidal cells in the CA3 area of the mouse hippocampus
    Akio Yamada
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 8:985-94. 2003
    ..S-SCAM has been suggested to be a component of PSDs, but its precise localization at the synapses remains unknown...
  42. ncbi request reprint Regulation of platelet-derived growth factor-induced Ras signaling by poliovirus receptor Necl-5 and negative growth regulator Sprouty2
    Mihoko Kajita
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Genes Cells 12:345-57. 2007
    ..Thus, Necl-5 and Sprouty2 cooperatively regulate PDGF-induced Ras signaling. The roles of Necl-5 and Sprouty2 in contact inhibition for cell proliferation are also discussed...
  43. ncbi request reprint Requirement of the actin cytoskeleton for the association of nectins with other cell adhesion molecules at adherens and tight junctions in MDCK cells
    Akio Yamada
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 9:843-55. 2004
    ..These results indicate that the actin cytoskeleton is required for the association of the nectin-afadin unit with other CAMs and PMPs at AJs and TJs...
  44. ncbi request reprint Regulation by nectin of the velocity of the formation of adherens junctions and tight junctions
    Tomoyuki Honda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka University, 565 0871, Suita, Japan
    Biochem Biophys Res Commun 306:104-9. 2003
    ..These results indicate that nectins regulate the velocity of the formation of the E-cadherin-based AJs and the subsequent formation of the claudin-based TJs...
  45. ncbi request reprint Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes
    Kotaro Wakamatsu
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 282:18173-81. 2007
    ..These results indicate that nectin-1 plays a key role in the expression of loricrin in the epidermis...
  46. ncbi request reprint Cdc42 and Rac small G proteins activated by trans-interactions of nectins are involved in activation of c-Jun N-terminal kinase, but not in association of nectins and cadherin to form adherens junctions, in fibroblasts
    Tomoyuki Honda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka University, Suita 565 0871, Japan
    Genes Cells 8:481-91. 2003
    ..We studied the roles of Cdc42 and Rac activated in this way in L fibroblasts stably expressing both nectin-1 and E-cadherin (nectin-1-EL cells)...
  47. ncbi request reprint Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division
    Masato Yasumi
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    J Biol Chem 280:6761-5. 2005
    ..NuMA complex. These results indicate that Lgl2 forms a Lgl2.Par-6.aPKC.LGN complex, which responds to mitotic signaling to establish normal cell division...
  48. ncbi request reprint Antagonistic and agonistic effects of an extracellular fragment of nectin on formation of E-cadherin-based cell-cell adhesion
    Tomoyuki Honda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 8:51-63. 2003
    ..To obtain evidence for this role of nectin, we attempted to develop an antagonist and/or agonist of nectin...
  49. ncbi request reprint Involvement of nectin-activated Cdc42 small G protein in organization of adherens and tight junctions in Madin-Darby canine kidney cells
    Atsunori Fukuhara
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 278:51885-93. 2003
    ..These results indicate that the Cdc42 activated by the trans-interactions of nectins is involved in the organization of AJs and TJs in different mechanisms in MDCK cells...
  50. ncbi request reprint Regulation of platelet-derived growth factor receptor activation by afadin through SHP-2: implications for cellular morphology
    Shinsuke Nakata
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka, Japan
    J Biol Chem 282:37815-25. 2007
    ..Thus, afadin plays a critical role in the proper regulation of the PDGF-induced activation of PDGF receptor and signaling by Ras-ERK. This effect, which is mediated by SHP-2, impacts cellular morphology...
  51. ncbi request reprint Roles and modes of action of nectins in cell-cell adhesion
    Kenji Irie
    Department of Molecular Biology and Biochemistry, Faculty of Medicine, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    Semin Cell Dev Biol 15:643-56. 2004
    ..We describe here the roles and modes of action of nectins as CAMs...
  52. ncbi request reprint The roles of nectins in cell adhesions: cooperation with other cell adhesion molecules and growth factor receptors
    Toshiaki Sakisaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 19:593-602. 2007
    ..Furthermore, nectin interacts in trans with nectin-like molecule-5 (Necl-5) and regulate cell movement and proliferation. We describe cooperative roles of nectins with other CAMs and growth factor receptors...
  53. ncbi request reprint Involvement of the annexin II-S100A10 complex in the formation of E-cadherin-based adherens junctions in Madin-Darby canine kidney cells
    Akio Yamada
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 280:6016-27. 2005
    ..Knockdown of annexin II by RNA interference also inhibited the re-concentration of E-cadherin. These results indicate that the annexin II-S100A10 complex is involved in the formation of the E-cadherin-based AJs in MDCK cells...
  54. ncbi request reprint Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions
    Takako Ooshio
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Sci 120:2352-65. 2007
    ..We describe here these novel roles of Par-3 in the formation of junctional complexes...
  55. ncbi request reprint Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion
    Tatsuhiro Sato
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine, Faculty of Medicine, Suita 565 0871, Japan
    Cancer Sci 96:578-89. 2005
    ..These results indicate that the trans-interactions of Necl-5 and nectin-3 and of nectin and nectin induce the activation of Cdc42 and Rac through the common signaling molecules c-Src, Rap1, FRG, and Vav2...
  56. ncbi request reprint Localization of mLin-7 at nectin-based cell-cell junctions
    Yasunori Yamamoto
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Oncogene 21:2545-54. 2002
    ..mLin-7 did not directly interact with nectin or afadin. The results indicate that mLin-7 localizes at cell-cell junctions through the nectin-afadin system...
  57. ncbi request reprint Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells
    Yukiko Minami
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    Biochem Biophys Res Commun 352:856-60. 2007
    ..In addition, expression of the gene encoding nectin-3 is markedly reduced in transformed cells. Thus, up-regulation of Necl-5 following transformation contributes to the loss of contact inhibition in transformed cells...
  58. ncbi request reprint Recruitment of E-cadherin associated with alpha- and beta-catenins and p120ctn to the nectin-based cell-cell adhesion sites by the action of 12-O-tetradecanoylphorbol-13-acetate in MDCK cells
    Ryoko Okamoto
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 10:435-45. 2005
    ....
  59. doi request reprint Structural and functional associations of apical junctions with cytoskeleton
    Jun Miyoshi
    Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    Biochim Biophys Acta 1778:670-91. 2008
    ..Here we describe how subcellular protein interactions are coordinated to induce changes in actin organization and dynamics, in response to the status of apical junctions...
  60. doi request reprint Establishment of cell polarity by afadin during the formation of embryoid bodies
    Hitomi Komura
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Genes Cells 13:79-90. 2008
    ..These results indicate that afadin organizes the formation of cell-cell junctions by regulating cell polarization in early embryonic development...
  61. ncbi request reprint Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions
    Tatsuhiro Sato
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 9:791-9. 2004
    ..These results indicate that Necl-5 plays roles not only in cell motility and proliferation but also in cell-cell adhesion in cooperation with nectin-3...
  62. ncbi request reprint Role of cell adhesion molecule nectin-3 in spermatid development
    Maiko Inagaki
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 11:1125-32. 2006
    ..These results indicate that the heterophilic trans-interaction between nectin-2 and nectin-3 is essential for the formation and maintenance of Sertoli-spermatid junctions that plays a critical role in spermatid development...
  63. pmc Involvement of afadin in barrier function and homeostasis of mouse intestinal epithelia
    Miki Tanaka-Okamoto
    Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537 8511, Japan
    J Cell Sci 124:2231-40. 2011
    ..These findings suggest that afadin plays crucial roles, independently of the role as the nectin-afadin module, in barrier function and homeostasis of the intestinal epithelia once the epithelial structure has been established...
  64. pmc Crystal Structure of the cis-Dimer of Nectin-1: implications for the architecture of cell-cell junctions
    Hirotaka Narita
    Laboratory of Supramolecular Crystallography, Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita 565 0871, Japan
    J Biol Chem 286:12659-69. 2011
    ..These results indicate that, in contrast with the previous notion, our structure represents a cis-dimer. Thus, our findings clearly reveal the structural basis for the cis-dimerization of nectins through the first Ig-like domains...
  65. ncbi request reprint Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development
    Maiko Inagaki
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Development 132:1525-37. 2005
    ....
  66. ncbi request reprint Transcriptional activation of the mouse Necl-5/Tage4/PVR/CD155 gene by fibroblast growth factor or oncogenic Ras through the Raf-MEK-ERK-AP-1 pathway
    Takeshi Hirota
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    Oncogene 24:2229-35. 2005
    ..These results indicate that the expression of mouse Necl-5 is induced by FGF or V12Ki-Ras through the Raf-MEK-ERK-AP-1 pathway...
  67. ncbi request reprint Novel role of nectin: implication in the co-localization of JAM-A and claudin-1 at the same cell-cell adhesion membrane domain
    Kaori Kuramitsu
    Department of Molecular Biology and Biochemistry, Osaka Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    Genes Cells 13:797-805. 2008
    ..Nectin-3 required afadin, a nectin- and F-actin-binding protein, for this ability. In conclusion, nectin plays a novel role in the co-localization of JAM and claudin at the same CAMD...
  68. pmc Involvement of the interaction of afadin with ZO-1 in the formation of tight junctions in Madin-Darby canine kidney cells
    Takako Ooshio
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 285:5003-12. 2010
    ..These results indicate that the transient interaction of afadin with ZO-1 is necessary for the formation of TJs in MDCK cells...
  69. ncbi request reprint Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells
    K Imazumi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan
    Biochem Biophys Res Commun 205:1409-16. 1994
    ..The amino acid sequence analysis of this 30 KDa protein revealed that it is GTP cyclohydrolase I...
  70. ncbi request reprint Association of ASIP/mPAR-3 with adherens junctions of mouse neuroepithelial cells
    Naoyuki Manabe
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama, Japan
    Dev Dyn 225:61-9. 2002
    ....
  71. ncbi request reprint Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions
    Kumi Ozaki-Kuroda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, 2 2 Yamada oka, Japan
    Curr Biol 12:1145-50. 2002
    ..These results indicate that the nectin-afadin system plays essential roles in coupling cell-cell adhesion and the cortical actin scaffold at SspJs and in subsequent sperm morphogenesis...
  72. ncbi request reprint Identification as beta-adducin of a protein interacting with rabphilin-3A in the presence of Ca2+ and phosphatidylserine
    M Miyazaki
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan
    Biochem Biophys Res Commun 205:460-6. 1994
    ..The Rabphilin-3A-interacting protein also binds to protein kinase C in the presence of Ca2+ and phosphatidylserine. These results indicate that Rabphilin-3A binds to beta-adducin in the presence of Ca2+ and phosphatidylserine...
  73. ncbi request reprint Localization of the Rab3 small G protein regulators in nerve terminals and their involvement in Ca2+-dependent exocytosis
    H Oishi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Japan
    J Biol Chem 273:34580-5. 1998
    ..These results indicate that Rab3 GEP and GAP are colocalized with Rab3A at the synaptic release sites and suggest that they regulate the activity of Rab3A and are involved in Ca2+-dependent exocytosis...
  74. ncbi request reprint [Role of the nectin-afadin system in organization of tight and adherens junctions in epithelial cells]
    Tatsuo Katata
    Tanpakushitsu Kakusan Koso 48:105-12. 2003
  75. ncbi request reprint Trans-interactions of nectins induce formation of filopodia and Lamellipodia through the respective activation of Cdc42 and Rac small G proteins
    Tomomi Kawakatsu
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 277:50749-55. 2002
    ..These effects of nectins require their cytoplasmic tail but not their association with afadin. We propose here the functional relationship between nectins and the small G proteins in the organization of AJs...
  76. ncbi request reprint Involvement of Cdc42 and Rac small G proteins in invadopodia formation of RPMI7951 cells
    Hirokazu Nakahara
    The First Department of Oral and Maxillofacial Surgery, Osaka University Graduate School of Dentistry, Suita 565 0871, Japan
    Genes Cells 8:1019-27. 2003
    ..We studied the roles of the Rho family small G proteins in invadopodia formation...
  77. ncbi request reprint Nectin and nectin-like molecules: biology and pathology
    Jun Miyoshi
    Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
    Am J Nephrol 27:590-604. 2007
    ..Here we summarize our knowledge of nectins and Necls and discuss cell adhesion biology in the kidney...
  78. ncbi request reprint Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process
    Y Fujita
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    Neuron 20:905-15. 1998
    ..These results suggest that tomosyn is an important component in the neurotransmitter release process where it may stimulate SNARE complex formation...
  79. pmc Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs
    N Masumoto
    Department of Obstetrics and Gynecology, Osaka University Medical School, Japan
    J Cell Biol 135:1741-7. 1996
    ..These results show that Rabphilin-3A is involved in Ca(2+)-dependent CG exocytosis at fertilization in mouse eggs...
  80. ncbi request reprint Dynamic observation of an atom-sized gold wire by phase electron microscopy
    Y Takai
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0879, Japan
    Phys Rev Lett 87:106105. 2001
    ..The gold wire was rather stable for a few seconds under strong electron beam illumination...
  81. ncbi request reprint Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae
    T Kamei
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Osaka, Japan
    J Biol Chem 273:28341-5. 1998
    ..These results indicate that Bnr1p directly interacts with Hof1p as well as with profilin to regulate cytoskeletal functions in S. cerevisiae...
  82. ncbi request reprint Role of nectin in organization of tight junctions in epithelial cells
    Atsunori Fukuhara
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    Genes Cells 7:1059-72. 2002
    ..We show here that nectin is also involved in the formation of TJs...
  83. doi request reprint Cross-talk among integrin, cadherin, and growth factor receptor: roles of nectin and nectin-like molecule
    Hisakazu Ogita
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Int Rev Cytol 265:1-54. 2008
    ....
  84. ncbi request reprint Role of multiple bonds between the single cell adhesion molecules, nectin and cadherin, revealed by high sensitive force measurements
    Yoshikazu Tsukasaki
    Department of Nanobiology, Graduate School of Frontier Biosciences, Osaka University, 1 3, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 367:996-1006. 2007
    ....
  85. ncbi request reprint [Role of nectins and nectin-like molecules in cell adhesion--a lesson on cell biology from the late Dr. Shoichiro Tsukita]
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Seikagaku 78:631-46. 2006
  86. pmc ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae
    H Hirano
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan
    Mol Cell Biol 16:4396-403. 1996
    ..These results indicate that Bem4p is a novel protein directly interacting with Rho1p and is involved in the RHO1-mediated signaling pathway...
  87. ncbi request reprint Three splicing variants of tomosyn and identification of their syntaxin-binding region
    S Yokoyama
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, 565 0871, Japan
    Biochem Biophys Res Commun 256:218-22. 1999
    ..This region was necessary but not sufficient for high-affinity binding of tomosyn to syntaxin-1...
  88. ncbi request reprint Direct interaction of the Rho GDP dissociation inhibitor with ezrin/radixin/moesin initiates the activation of the Rho small G protein
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan
    J Biol Chem 272:23371-5. 1997
    ..These results suggest that ERM as well as Rho GDI and the Rho GDP/GTP exchange factor are involved in the activation of the Rho subfamily members, which then regulate reorganization of actin filaments through the ERM system...
  89. ncbi request reprint Phosphorylation of Munc-18/n-Sec1/rbSec1 by protein kinase C: its implication in regulating the interaction of Munc-18/n-Sec1/rbSec1 with syntaxin
    Y Fujita
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    J Biol Chem 271:7265-8. 1996
    ....
  90. ncbi request reprint Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling
    A Mammoto
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Japan
    J Biol Chem 274:25517-24. 1999
    ..Rabphilin-11 turned out to be a rat counterpart of recently reported bovine Rab11BP. These results indicate that rabphilin-11 is a downstream target of Rab11 which is involved in vesicle recycling...
  91. ncbi request reprint Interaction of radixin with Rho small G protein GDP/GTP exchange protein Dbl
    K Takahashi
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    Oncogene 16:3279-84. 1998
    ..Thus, radixin plays an important role in activation of the Rho family members by recruiting their positive and negative regulators...
  92. pmc Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae
    T Fujiwara
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565 0871, Japan
    Mol Biol Cell 9:1221-33. 1998
    ..These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p...
  93. pmc Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae
    H Imamura
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    EMBO J 16:2745-55. 1997
    ..These results indicate that Bni1p and Bnr1p are potential targets of the Rho family members, interact with profilin and regulate the reorganization of actin cytoskeleton...
  94. pmc Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae
    H Kohno
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    EMBO J 15:6060-8. 1996
    ..Furthermore, this synthetic lethality was caused by the incapability of RhoA to activate Pkc1p, but not glucan synthase. These results suggest that Rho1p regulates cytoskeletal reorganization at least through Bni1p and Pkc1p...
  95. ncbi request reprint Physical and functional interaction of rabphilin-11 with mammalian Sec13 protein. Implication in vesicle trafficking
    A Mammoto
    Department of Molecular Biology, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Biol Chem 275:13167-70. 2000
    ..These results indicate that the rabphilin-11-mSec13p interaction is implicated in vesicle trafficking...
  96. pmc A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae
    H Nonaka
    Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan
    EMBO J 14:5931-8. 1995
    ..These results indicate that there are at least two signaling pathways regulated by Rho1p and that one of the downstream targets is Pkc1p, leading to the activation of the MAP kinase cascade...
  97. ncbi request reprint Ectodomain shedding of nectin-1alpha by SF/HGF and TPA in MDCK cells
    Yoshinari Tanaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    Biochem Biophys Res Commun 299:472-8. 2002
    ....
  98. ncbi request reprint Activation of Rap1, Cdc42, and rac by nectin adhesion system
    Hisakazu Ogita
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine, Japan
    Methods Enzymol 406:415-24. 2006
    ..Here, we focus on how the activity of Cdc42 and Rac induced by trans-interacting nectin is examined by use of the pull-down and the cell-spreading assays...
  99. ncbi request reprint Nectins and nectin-like molecules: roles in cell adhesion, polarization, movement, and proliferation
    Hisakazu Ogita
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka, Japan
    IUBMB Life 58:334-43. 2006
    ..Thus, both nectin and Necl family members play important roles in fundamental cellular functions, including cell adhesion, polarization, movement, and proliferation...
  100. ncbi request reprint Involvement of nectin in inactivation of integrin alpha(v)beta(3) after the establishment of cell-cell adhesion
    Yasuhisa Sakamoto
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 283:496-505. 2008
    ..Therefore, the trans-interaction of nectin essentially functions in the inactivation of integrin at AJs through the PTPmu-induced inactivation of PIPKIgamma...
  101. doi request reprint A cell-free assay for endocytosis of E-cadherin
    Toshiaki Sakisaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine, Suita, Japan
    Methods Mol Biol 440:77-87. 2008
    ..Here, we describe the assay method for the endocytosis of E-cadherin from the AJ-enriched fraction prepared from rat liver...