Chikashi Shimoda

Summary

Affiliation: Osaka City University
Country: Japan

Publications

  1. ncbi request reprint Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking
    Chikashi Shimoda
    Department of Biology, Graduate School of Science, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    J Cell Sci 117:389-96. 2004
  2. ncbi request reprint [Yeast and its genetic manipulation]
    Chikashi Shimoda
    Tanpakushitsu Kakusan Koso 49:1558-64. 2004
  3. ncbi request reprint Isolation of thermotolerant mutants by using proofreading-deficient DNA polymerase delta as an effective mutator in Saccharomyces cerevisiae
    Chikashi Shimoda
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Genes Genet Syst 81:391-7. 2006
  4. pmc Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast
    Yukiko Nakase
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 19:2476-87. 2008
  5. pmc The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis
    Michiko Nakamura-Kubo
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Mol Biol Cell 22:3442-55. 2011
  6. pmc Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane
    Akiko Itadani
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    Eukaryot Cell 9:1925-35. 2010
  7. pmc Live observation of forespore membrane formation in fission yeast
    Taro Nakamura
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 19:3544-53. 2008
  8. ncbi request reprint Two fission yeast rab7 homologs, ypt7 and ypt71, play antagonistic roles in the regulation of vacuolar morphology
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Traffic 10:912-24. 2009
  9. pmc Geranylgeranyl diphosphate synthase in fission yeast is a heteromer of farnesyl diphosphate synthase (FPS), Fps1, and an FPS-like protein, Spo9, essential for sporulation
    Yanfang Ye
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Mol Biol Cell 18:3568-81. 2007
  10. pmc Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 22:3658-70. 2011

Collaborators

  • Taro Nakamura
  • Kaoru Takegawa
  • Yasushi Hiraoka
  • Kenji Kitamura
  • Koei Okazaki
  • Yuko Giga-Hama
  • Yasufumi Kaneda
  • Manabu Sato
  • Anthony M Carr
  • Yasuhisa Fukui
  • Aiko Hirata
  • Jun Kashiwazaki
  • Yukiko Nakase
  • Akiko Itadani
  • Taisuke Seike
  • Michiko Nakamura-Kubo
  • Kana Fukunishi
  • Shu Hei Yoshida
  • Yukari Maeda
  • Takako Koga
  • Yanfang Ye
  • Tomoko Iwaki
  • Tomomi Yamaoka
  • Yuriko Yamasaki
  • Masayuki Onishi
  • Masayo Morishita
  • Cong Liu
  • Naotaka Tanaka
  • Yoko Nakamura
  • Kana Miyakubi
  • Mitsuko Hatanaka
  • Natsumi Otsuru
  • Katsuyuki K Tamai
  • Kazuki Imada
  • Hideo Iio
  • Yoshikazu Yamagishi
  • Erika Teraguchi
  • Makoto Kawamukai
  • Makoto Fujii
  • Olaf Nielsen
  • Marius Poitelea
  • Yasuko Fujita
  • Adam Watson
  • Christian Holmberg
  • Shotaro Suzuki
  • Christopher J McInerny
  • Hiba Al-Amodi
  • Rika Morita
  • Hiromi Maekawa
  • Ichiro Yamashita
  • Fusako Morimoto

Detail Information

Publications32

  1. ncbi request reprint Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking
    Chikashi Shimoda
    Department of Biology, Graduate School of Science, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    J Cell Sci 117:389-96. 2004
    ..Understanding these dynamic aspects of yeast sporulation will help to elucidate a general mechanism for the cellularization of cytoplasm containing multiple nuclei...
  2. ncbi request reprint [Yeast and its genetic manipulation]
    Chikashi Shimoda
    Tanpakushitsu Kakusan Koso 49:1558-64. 2004
  3. ncbi request reprint Isolation of thermotolerant mutants by using proofreading-deficient DNA polymerase delta as an effective mutator in Saccharomyces cerevisiae
    Chikashi Shimoda
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Genes Genet Syst 81:391-7. 2006
    ..These results indicate that a proofreading-deficient DNA delta polymerase variant is an effective mutator for obtaining yeast mutants that have gained useful characteristics, such as the ability to proliferate in harsh environments...
  4. pmc Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast
    Yukiko Nakase
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 19:2476-87. 2008
    ..Taken together, these observations indicate that Spo2 is recruited to the SPB during meiosis and then assists in the localization of Spo13 to the outer surface of the SPB...
  5. pmc The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis
    Michiko Nakamura-Kubo
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Mol Biol Cell 22:3442-55. 2011
    ..Our study suggests that Spo7 coordinates formation of the leading edge and initiation of FSM assembly, thereby accomplishing accurate formation of the FSM...
  6. pmc Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane
    Akiko Itadani
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    Eukaryot Cell 9:1925-35. 2010
    ..These results indicate that calmodulin plays an essential role in forespore membrane formation by stably maintaining Spo15, and thus Spo2 and Spo13, at the SPB in meiotic cells...
  7. pmc Live observation of forespore membrane formation in fission yeast
    Taro Nakamura
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 19:3544-53. 2008
    ..These observations suggest that FSM expansion is composed of two steps, early meiotic FSM expansion and late and post meiotic FSM expansion. Possible regulatory mechanisms of FSM formation in fission yeast are discussed...
  8. ncbi request reprint Two fission yeast rab7 homologs, ypt7 and ypt71, play antagonistic roles in the regulation of vacuolar morphology
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Traffic 10:912-24. 2009
    ..Analysis of a chimeric Ypt7/Ypt71 protein showed that Rab7-directed vacuole dynamics, fusion versus fission, largely depends on the medial region of the protein, including a part of RabSF3/alpha3-L7...
  9. pmc Geranylgeranyl diphosphate synthase in fission yeast is a heteromer of farnesyl diphosphate synthase (FPS), Fps1, and an FPS-like protein, Spo9, essential for sporulation
    Yanfang Ye
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Mol Biol Cell 18:3568-81. 2007
    ..We suggest that lack of GGPS activity in a spo9 mutant results in impaired protein prenylation in certain proteins responsible for secretory function, thereby inhibiting forespore membrane formation...
  10. pmc Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Mol Biol Cell 22:3658-70. 2011
    ..We consider that during meiosis in Schizosaccharomyces pombe cells, the characteristics of endocytosis change, thereby facilitating internalization of Psy1 and accomplishing sporulation...
  11. pmc Ectopic overproduction of a sporulation-specific transcription factor induces assembly of prespore-like membranous compartments in vegetative cells of fission yeast
    Yukiko Nakase
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    Genetics 183:1195-9. 2009
    ..These results suggest why expression of development-specific transcription factors must be strictly controlled...
  12. pmc The fission yeast spo14+ gene encoding a functional homologue of budding yeast Sec12 is required for the development of forespore membranes
    Michiko Nakamura-Kubo
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Japan
    Mol Biol Cell 14:1109-24. 2003
    ..We conclude that Spo14 is responsible for the assembly of the forespore membrane by supplying membrane vesicles...
  13. ncbi request reprint The Schizosaccharomyces pombe syntaxin 1 homolog, Psy1, is essential in the development of the forespore membrane
    Yukari Maeda
    Department of Biology, Graduate School of Science, Osaka City University, Japan
    Biosci Biotechnol Biochem 73:339-45. 2009
    ..These results indicate that Psy1 plays an essential role in FSM formation coordinated by Syb1 and Sec9...
  14. ncbi request reprint A fission yeast SNAP-25 homologue, SpSec9, is essential for cytokinesis and sporulation
    Taro Nakamura
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Cell Struct Funct 30:15-24. 2005
    ..However, subsequent extension of the membrane was severely impaired. These results indicate that SpSec9 plays an important role both in cytokinesis and in sporulation...
  15. pmc The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation
    Tomomi Yamaoka
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    Eukaryot Cell 12:1162-70. 2013
    ..Taken together, these results indicate that Syb1 plays an important role in both FSM assembly and spore wall formation. ..
  16. ncbi request reprint The Sec14 family glycerophospholipid-transfer protein is required for structural integrity of the spindle pole body during meiosis in fission yeast
    Yukiko Nakase
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    Genes Cells 9:1275-86. 2004
    ..We conclude that Spo20 plays a role in maintaining the structural integrity of the meiotic SPB, besides supplying membrane vesicles for forespore membrane assembly...
  17. pmc The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication
    Shu Hei Yoshida
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Genetics 164:881-93. 2003
    ..These observations suggest that cdt2(+) plays an important role in DNA replication in both the mitotic and the meiotic life cycles of fission yeast...
  18. ncbi request reprint The cation-transporting P-type ATPase Cta4 is required for assembly of the forespore membrane in fission yeast
    Shu Hei Yoshida
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Japan
    Genes Genet Syst 80:317-24. 2005
    ..Consequently, formation of the forespore membrane was severely impaired. These observations show that perturbation of Ca2+ homeostasis by mutation of cta4/sev4 blocks sporulation mainly by interfering with forespore membrane assembly...
  19. ncbi request reprint Localization of type I myosin and F-actin to the leading edge region of the forespore membrane in Schizosaccharomyces pombe
    Akiko Itadani
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Cell Struct Funct 31:181-95. 2006
    ..In addition, both F-actin and Myo1 localized with Cam2 in the leading edge region. In summary, type I myosin and F-actin accumulate at the leading edge area of the forespore membrane and may play a pivotal role in its assembly...
  20. ncbi request reprint A role for fission yeast Rab GTPase Ypt7p in sporulation
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Cell Struct Funct 30:43-9. 2005
    ..Vacuoles in sporulating cells were found to undergo extensive homotypic vacuole fusion to form a few large compartments occupying the entire cytoplasm of asci. This extensive vacuole fusion depended on Ypt7p...
  21. pmc Distal and proximal actions of peptide pheromone M-factor control different conjugation steps in fission yeast
    Taisuke Seike
    Department of Biology, Graduate School of Science, Osaka City University, Osaka, Japan
    PLoS ONE 8:e69491. 2013
    ....
  22. ncbi request reprint Phosphatidylinositol 3-phosphate 5-kinase is required for the cellular response to nutritional starvation and mating pheromone signals in Schizosaccharomyces pombe
    Masayo Morishita
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Genes Cells 7:199-215. 2002
    ..A homologous protein, SpFab1p, has been found in the fission yeast Schizosaccharomyces pombe, but its role is not known...
  23. pmc Remarkably simple sequence requirement of the M-factor pheromone of Schizosaccharomyces pombe
    Taisuke Seike
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Genetics 191:815-25. 2012
    ..At least 15 nonfunctional peptides were found to be secreted, suggesting that these mutant M-factor peptides are no longer recognized by the cognate receptor...
  24. pmc Novel fission yeast Cdc7-Dbf4-like kinase complex required for the initiation and progression of meiotic second division
    Taro Nakamura
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Osaka, Japan
    Mol Cell Biol 22:309-20. 2002
    ..These results suggest that Spo4 is a Cdc7 kinase whose primary role is in meiosis, not in DNA replication. This is the first report of an organism which has two Cdc7-related kinase complexes with different biological functions...
  25. ncbi request reprint The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3
    Kana Fukunishi
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Mol Biol Cell 25:1549-59. 2014
    ..This layer is not observed in isp3∆ spores. Furthermore, Isp3 is abundantly detected in this layer by immunoelectron microscopy. Thus Isp3 constitutes the spore coat, thereby conferring resistance to various environmental stresses. ..
  26. ncbi request reprint The novel HECT-type ubiquitin-protein ligase Pub2p shares partially overlapping function with Pub1p in Schizosaccharomyces pombe
    Katsuyuki K Tamai
    Department of Biology, Graduate School of Science, Osaka City University, Sugimoto 3 3 138, Sumiyoshi ku, Osaka 558 8585, Japan
    J Cell Sci 115:1847-57. 2002
    ..We conclude that Pub2p is a HECT-type ubiquitin-protein ligase that shares partially overlapping function with Pub1p...
  27. pmc Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase
    Cong Liu
    Genome Damage and Stability Centre, University of Sussex, Brighton, UK
    EMBO J 24:3940-51. 2005
    ..We propose that Cdt2 incorporation into the Pcu4-Ddb1-CSN complex prompts Spd1 targeting and subsequent degradation and that Cdt2 is a WD40 repeat adaptor protein for Cullin-4-based ubiquitin ligase...
  28. ncbi request reprint Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe
    Naotaka Tanaka
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Biochem Biophys Res Commun 330:813-20. 2005
    ..These results indicate that O-glycosylation initiated by Ogm proteins plays crucial physiological roles and can serve as a sorting determinant for protein transport of membrane glycoproteins in S. pombe...
  29. ncbi request reprint Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe
    Takako Koga
    Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Genes Cells 9:561-74. 2004
    ..These results suggest that Vps5p and Vps17p are the targets of PtdIns(3)P in vesicle transport required for onset of the forespore membrane formation...
  30. pmc ADAM family protein Mde10 is essential for development of spore envelopes in the fission yeast Schizosaccharomyces pombe
    Tomohiro Nakamura
    Faculty of Intellectual Property, Osaka Institute of Technology, Asahi ku, Osaka 535 8585, Japan
    Eukaryot Cell 3:27-39. 2004
    ..Our observations indicate that Mde10 is important in the development of the spore envelope, although this function of Mde10 seems to be independent of its metalloprotease activity...
  31. ncbi request reprint Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe
    Masayuki Onishi
    Laboratory of Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Yeast 20:193-206. 2003
    ..pombe...
  32. ncbi request reprint Ribosomal proteins S0 and S21 are involved in the stability of 18S rRNA in fission yeast, Schizosaccharomyces pombe
    Manabu Sato
    Department of Gene Therapy Science, Osaka University Graduate School of Medicine, Yamada oka 2 2, 565 0871, Suita, Japan
    Biochem Biophys Res Commun 311:942-7. 2003
    ..Additional functional studies indicate that this phenomenon is likely to be caused by insufficient 18S rRNA stability. The possible role of Rps0p and Rps21 that contribute to 18S rRNA maturation is further discussed...