Shinji Sakai

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan Electronic address
    Carbohydr Polym 111:404-9. 2014
  2. doi request reprint Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication
    Shinji Sakai
    Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, Japan
    Biofabrication 5:015012. 2013
  3. doi request reprint Enzymatically-gelled amylopectin-based substrates enable on-demand harvesting cells with preserving cell-to-cell connection using saliva
    Shinji Sakai
    Department of Materials Science and Engineering, Osaka University, Toyonaka, Osaka, Japan
    J Biosci Bioeng 115:462-5. 2013
  4. doi request reprint Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
    Cancer Sci 103:549-54. 2012
  5. doi request reprint Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, Japan
    Biotechnol Bioeng 109:2911-9. 2012
  6. doi request reprint Wrapping tissues with a pre-established cage-like layer composed of living cells
    Shinji Sakai
    Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan
    Biomaterials 33:6721-7. 2012
  7. ncbi request reprint In situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, 560 8531, Japan
    Biomacromolecules 11:1370-5. 2010
  8. doi request reprint Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, 560 8531, Japan
    Biomacromolecules 11:2179-83. 2010
  9. ncbi request reprint Production of butyl-biodiesel using lipase physically-adsorbed onto electrospun polyacrylonitrile fibers
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    Bioresour Technol 101:7344-9. 2010
  10. doi request reprint Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan
    Biotechnol Lett 32:1059-62. 2010

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan Electronic address
    Carbohydr Polym 111:404-9. 2014
    ..Fibroblastic cells did not adhere on the former hydrogel but adhered and spread on the latter one. The growth of Escherichia coli cells was fully suppressed on the latter hydrogel during 48h cultivation. ..
  2. doi request reprint Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication
    Shinji Sakai
    Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, Japan
    Biofabrication 5:015012. 2013
    ..A filament-like structure of HeLa cells covered with a layer of fibroblast 10T1/2 cells was obtained when confluency of fibroblast 10T1/2 cells was reached and the hydrogel matrix was degraded with alginate lyase...
  3. doi request reprint Enzymatically-gelled amylopectin-based substrates enable on-demand harvesting cells with preserving cell-to-cell connection using saliva
    Shinji Sakai
    Department of Materials Science and Engineering, Osaka University, Toyonaka, Osaka, Japan
    J Biosci Bioeng 115:462-5. 2013
    ..The human epithelial cells grown on the surface of the hydrogels detached within 10 min with preserving cell-to-cell connection by soaking in the medium containing human saliva at 5% (v/v)...
  4. doi request reprint Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
    Cancer Sci 103:549-54. 2012
    ..09). These findings suggest the duplex microcapsule may be a promising tool for producing MTS for pharmaceutical applications...
  5. doi request reprint Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, Japan
    Biotechnol Bioeng 109:2911-9. 2012
    ..An endothelial cell network was formed by cell migration into the collagen gel. These results demonstrate the potential of serum-degradable AP-Ph hydrogels in constructing three-dimensional tissues...
  6. doi request reprint Wrapping tissues with a pre-established cage-like layer composed of living cells
    Shinji Sakai
    Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan
    Biomaterials 33:6721-7. 2012
    ..This method was also effective for wrapping multiple cellular aggregates within a single cage of HAE cells. In addition, it was possible to wrap tissue grown from individual cells in spherical cavities within the microcapsules...
  7. ncbi request reprint In situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, 560 8531, Japan
    Biomacromolecules 11:1370-5. 2010
    ....
  8. doi request reprint Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, 560 8531, Japan
    Biomacromolecules 11:2179-83. 2010
    ..The versatility of hematin for gelation of a variety of polymers possessing Ph groups was demonstrated by the gelation of a carboxymethyl cellulose derivative...
  9. ncbi request reprint Production of butyl-biodiesel using lipase physically-adsorbed onto electrospun polyacrylonitrile fibers
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    Bioresour Technol 101:7344-9. 2010
    ....
  10. doi request reprint Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan
    Biotechnol Lett 32:1059-62. 2010
    ..After 10 recyclings, the initial reaction rate was 80% of the original rate. This system of enzyme immobilization is therefore suitable for carrying out transesterification reactions in nonaqueous solvents...
  11. doi request reprint Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process
    Yang Liu
    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka 560 8531, Japan
    J Biosci Bioeng 114:353-9. 2012
    ..Human smooth muscle cells adhered, grew and achieved confluence on the surface of the hydrogel fibers. By degrading the hydrogel fibers using alginate lyase, a tubular cell construct was successfully obtained...
  12. doi request reprint Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths
    Yang Liu
    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    Acta Biomater 9:6616-23. 2013
    ..Finally, spherical tissues of 10T1/2 cells wrapped in living HAE cell sheaths were obtained by treatment with alginate lyase...
  13. doi request reprint Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel
    Sho Shinohara
    Graduate School of Frontier Biosciences, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 116:231-4. 2013
    ..Using the layer-by-layer cell multilayered technique, we successfully constructed an artificial blood vessel...
  14. doi request reprint Enhanced productivity of electrospun polyvinyl alcohol nanofibrous mats using aqueous N,N-dimethylformamide solution and their application to lipase-immobilizing membrane-shaped catalysts
    Koichi Sawada
    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    J Biosci Bioeng 114:204-8. 2012
    ....
  15. doi request reprint Horseradish peroxidase-mediated encapsulation of mammalian cells in hydrogel particles by dropping
    Shinji Sakai
    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    J Microencapsul 31:100-4. 2014
    ..These results demonstrate that we successfully established the method of cell-encapsulation in hydrogel particles based on dropping aqueous polymer solution into aqueous coagulation solution through HRP-catalysed reaction...
  16. doi request reprint Competing two enzymatic reactions realizing one-step preparation of cell-enclosing duplex microcapsules
    Tomoaki Ashida
    Div of Chemical Engineering, Dept of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama cho, Toyonaka, Osaka, 560 8531, Japan
    Biotechnol Prog 29:1528-34. 2013
    ..The proposed method could be versatile for preparing the microcapsules from the other polymer derivatives of carboxymetylcellulose and gelatin...