K Ozawa

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint 150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death
    K Ozawa
    Department of Anatomy and Neuroscience, Biomedical Research Center, Osaka University Medical School, 2 2 Yamada oka, Suita City 565, Japan
    J Biol Chem 274:6397-404. 1999
  2. ncbi request reprint 150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells
    Y Bando
    Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Suita City, Japan
    Am J Physiol Cell Physiol 278:C1172-82. 2000
  3. ncbi request reprint ORP150 protects against hypoxia/ischemia-induced neuronal death
    M Tamatani
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka, Japan
    Nat Med 7:317-23. 2001
  4. ncbi request reprint Contact dermatitis due to pirfenoxone
    S Inui
    Department of Dermatology, Unit of Molecular Medicine, Graduate School of Medicine, Osaka University, 2 2, C 5, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    Contact Dermatitis 50:375-6. 2004
  5. ncbi request reprint Stimulation of GATA-2 as a mechanism of hydrogen peroxide suppression in hypoxia-induced erythropoietin gene expression
    M Tabata
    Department of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi, Japan
    J Cell Physiol 186:260-7. 2001
  6. ncbi request reprint Antitumor effect of reduction of 150-kDa oxygen-regulated protein expression in human prostate cancer cells
    T Miyagi
    Department of Urology, Kanazawa University School of Medicine, Kanazawa, Japan
    Mol Urol 5:79-80. 2001
  7. ncbi request reprint Baseball pitcher's friction dermatitis
    S Inui
    Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University, 2 2 C 5 Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    Contact Dermatitis 47:176-7. 2002
  8. pmc Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicity
    Y Kitao
    Department of Neuroanatomy, Kanazawa University Medical School, Kanazawa City, Ishikawa, Japan
    J Clin Invest 108:1439-50. 2001
  9. ncbi request reprint Purification and characterization of homo- and hetero-dimeric acetate kinases from the sulfate-reducing bacterium Desulfovibrio vulgaris
    L Yu
    Department of Biochemistry, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-2192, Japan
    J Biochem 129:411-21. 2001
  10. ncbi request reprint Enhanced Abeta40 deposition was associated with increased Abeta42-43 in cerebral vasculature with Dutch-type hereditary cerebral hemorrhage with amyloidosis (HCHWA-D)
    K Ozawa
    Department of Neuroscience, Institute of Gerontology, Osaka City University, 1 4 3 Asahimachi, Abenoku, Osaka 545 8585, Japan
    Ann N Y Acad Sci 977:149-54. 2002

Collaborators

Detail Information

Publications35

  1. ncbi request reprint 150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death
    K Ozawa
    Department of Anatomy and Neuroscience, Biomedical Research Center, Osaka University Medical School, 2 2 Yamada oka, Suita City 565, Japan
    J Biol Chem 274:6397-404. 1999
    ....
  2. ncbi request reprint 150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells
    Y Bando
    Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Suita City, Japan
    Am J Physiol Cell Physiol 278:C1172-82. 2000
    ..These data indicate that ORP150 may function as a unique molecular chaperone in renal epithelial cells by facilitating protein transport/maturation in an environment where less ATP is accessible...
  3. ncbi request reprint ORP150 protects against hypoxia/ischemia-induced neuronal death
    M Tamatani
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka, Japan
    Nat Med 7:317-23. 2001
    ..These data indicate that ORP150 is an integral participant in ischemic cytoprotective pathways...
  4. ncbi request reprint Contact dermatitis due to pirfenoxone
    S Inui
    Department of Dermatology, Unit of Molecular Medicine, Graduate School of Medicine, Osaka University, 2 2, C 5, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    Contact Dermatitis 50:375-6. 2004
  5. ncbi request reprint Stimulation of GATA-2 as a mechanism of hydrogen peroxide suppression in hypoxia-induced erythropoietin gene expression
    M Tabata
    Department of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi, Japan
    J Cell Physiol 186:260-7. 2001
    ..From these results, we conclude that H2O2 increases the binding activity of GATA-2 in a specific manner, thereby suppressing the activity of the Epo promoter and thus inhibiting Epo gene expression...
  6. ncbi request reprint Antitumor effect of reduction of 150-kDa oxygen-regulated protein expression in human prostate cancer cells
    T Miyagi
    Department of Urology, Kanazawa University School of Medicine, Kanazawa, Japan
    Mol Urol 5:79-80. 2001
    ..We have examined whether transduced antisense ORP150 cDNA reduces tumorigenicity and angiogenicity. Relations between these stress proteins and cancer and possibilities for anticancer gene therapy are described...
  7. ncbi request reprint Baseball pitcher's friction dermatitis
    S Inui
    Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University, 2 2 C 5 Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    Contact Dermatitis 47:176-7. 2002
  8. pmc Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicity
    Y Kitao
    Department of Neuroanatomy, Kanazawa University Medical School, Kanazawa City, Ishikawa, Japan
    J Clin Invest 108:1439-50. 2001
    ..Our data underscore a possible role for ER stress in glutamate toxicity and pinpoint a key ER chaperone, ORP150, which contributes to the stress response critical for neuronal survival...
  9. ncbi request reprint Purification and characterization of homo- and hetero-dimeric acetate kinases from the sulfate-reducing bacterium Desulfovibrio vulgaris
    L Yu
    Department of Biochemistry, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-2192, Japan
    J Biochem 129:411-21. 2001
    ..These results suggest that two forms of acetate kinase are produced to finely regulate the enzyme function by post-translational modifications of a primary gene product in Desulfovibrio vulgaris...
  10. ncbi request reprint Enhanced Abeta40 deposition was associated with increased Abeta42-43 in cerebral vasculature with Dutch-type hereditary cerebral hemorrhage with amyloidosis (HCHWA-D)
    K Ozawa
    Department of Neuroscience, Institute of Gerontology, Osaka City University, 1 4 3 Asahimachi, Abenoku, Osaka 545 8585, Japan
    Ann N Y Acad Sci 977:149-54. 2002
    ..Taken together, amyloid angiopathy in HCHWA-D may share an Abeta(42)-driven deposition mechanism with plaque amyloid, resulting in enhanced Abeta(40) deposition...
  11. ncbi request reprint A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c
    K Ozawa
    Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Japan
    Biosci Biotechnol Biochem 65:185-9. 2001
    ..coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones...
  12. ncbi request reprint Antitumor effect of beta2-microglobulin in leukemic cell-bearing mice via apoptosis-inducing activity: activation of caspase-3 and nuclear factor-kappaB
    M Mori
    Department of Hematology, Jichi Medical School, Tochigi 329-04, Japan
    Cancer Res 61:4414-7. 2001
    ..We conclude that beta2m stimulates caspase-3 and NF-kappaB pathways to induce apoptosis, making it a useful approach to a new therapy for leukemia...
  13. ncbi request reprint Glutathione S-transferase Pi is a dopamine-inducible suppressor of dopamine-induced apoptosis in PC12 cells
    A Ishisaki
    Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST, Tsukuba, Ibaraki, Japan
    J Neurochem 77:1362-71. 2001
    ..These results indicate that GSTp is a dopamine-inducible suppressor of dopamine-induced apoptosis in PC12 cells, and suggest that this activity is exerted through inhibition of JNK activity...
  14. ncbi request reprint Morphological and physiological restorations of hereditary form of dilated cardiomyopathy by somatic gene therapy
    T Kawada
    Pharmacy Division, Niigata University, Japan
    Biochem Biophys Res Commun 284:431-5. 2001
    ..These results provide the first evidence that supplementation of a specific gene with efficient and sustained transfection capability restores the genetic, morphological, and functional deteriorations...
  15. ncbi request reprint Recombinant adeno-associated virus vector-transduced vascular endothelial cells express the thrombomodulin transgene under the regulation of enhanced plasminogen activator inhibitor-1 promoter
    J Mimuro
    Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical School, Tochigi-Ken Japan
    Gene Ther 8:1690-7. 2001
    ..Transduction of endothelial cells by rAAV vectors to express enhanced PAI-1 promoter-driven transgenes may be a useful gene therapy approach for vascular diseases...
  16. ncbi request reprint Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells
    K Ohmine
    Division of Functional Genomics, Jichi Medical School, Kawachi gun, Tochigi 329 0498, Japan
    Oncogene 20:8249-57. 2001
    ..These data suggest that microarray analysis with background-matched samples is an efficient approach to identify molecular events underlying the stage progression in CML...
  17. ncbi request reprint Functions and ATP-binding responses of the twelve histidine residues in the TF1-ATPase beta subunit
    K Tozawa
    Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama 240-8501, Japan
    J Biochem 130:527-33. 2001
    ..1999) Biophys. J. 77, 2175-2183]. This conformational change should be one of the essential driving forces in the rotation of the alpha3beta3gamma complex...
  18. ncbi request reprint Fanconi anemia protein, FANCA, associates with BRG1, a component of the human SWI/SNF complex
    T Otsuki
    Department of Hematology, Jichi Medical School, Yakushiji 3311 1, Minamikawachi, Kawachi, Tochigi 329 0498, Japan
    Hum Mol Genet 10:2651-60. 2001
    ....
  19. ncbi request reprint Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components
    K Kogure
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami-kawachi, Tochigi, Japan
    Int J Hematol 73:469-75. 2001
    ..These results suggest that site-specific integration of foreign DNA can be achieved at a significantly high rate in human hematopoietic cells using the AAV components...
  20. pmc Downregulation of Bim, a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-initiated survival signaling in murine hematopoietic progenitors
    T Shinjyo
    Departments of Molecular Biology, Jichi Medical School, Tochigi 329-0498, Japan
    Mol Cell Biol 21:854-64. 2001
    ..We conclude that Bim is a pivotal molecule in cytokine regulation of hematopoietic cell survival...
  21. ncbi request reprint Molecular cloning and characterization of G-CSF induced gene cDNA
    M Shimane
    Institute of Medical Science, University of Tokyo, Japan
    Biochem Biophys Res Commun 199:26-32. 1994
    ..One of these clones, GIG-1 (G-CSF-induced gene-1), was further characterized. The size of the GIG-1 mRNA was about 0.9kb. The GIG-1 mRNA was expressed mainly in the myeloid leukemic cell lines...
  22. ncbi request reprint Production of human granulocyte colony stimulating factor by various kinds of stromal cells in vitro detected by enzyme immunoassay and in situ hybridization
    K Watari
    Department of Hematology Oncology, University of Tokyo, Japan
    Stem Cells 12:416-23. 1994
    ..Although further characterization of positive cells is needed, the results suggest that bone marrow stromal cells are heterogeneous in terms of their capacity for G-CSF production...
  23. ncbi request reprint Tec protein-tyrosine kinase is an effector molecule of Lyn protein-tyrosine kinase
    H Mano
    Department of Molecular Biology, Jichi Medical School, Tochigi ken, Japan
    FASEB J 10:637-42. 1996
    ..From this evidence we conclude that Tec acts downstream of Lyn in intracellular signaling pathways. This is a novel case where one PTK is phosphorylated and regulated by another...
  24. ncbi request reprint Cloning and characterization of mouse tec promoter
    H Honda
    Department of Molecular Biology, Institute of Hematology, Jichi Medical School, Tochigi ken, Japan
    Biochem Biophys Res Commun 223:422-6. 1996
    ..The promoter activity of the 5' flanking region was confirmed by using the luciferase assay. The present analysis will provide insights into the mechanism for the expression and regulation of the Tec molecule...
  25. ncbi request reprint SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase
    K i Ohya
    Division of Cardiology, Jichi Medical School, 3311 1 Yakushiji, Kawachi gun, Tochigi 329 04, Japan
    J Biol Chem 272:27178-82. 1997
    ..Interestingly, TIP3 can also down-regulate the activity of Jak2 but not that of Lyn. We propose that SOCS-1/JAB/SSI-1/TIP3 is a novel type of negative regulator to a subset of protein-tyrosine kinases...
  26. ncbi request reprint Tec and Jak2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription
    Y Yamashita
    Department of Molecular Biology, Jichi Medical School, Tochigi, Japan
    Blood 91:1496-507. 1998
    ..Thus, Tec and Jak2 can "cross-talk" in a complexed way to mediate cytokine-driven c-fos activation...
  27. ncbi request reprint Expression of the 150-kd oxygen-regulated protein in human breast cancer
    Y Tsukamoto
    Department of Pathology, National Cardiovascular Center, Suita City, Japan
    Lab Invest 78:699-706. 1998
    ..Furthermore, pancreatic and thyroid carcinomas also displayed greater ORP150 expression. These results suggest that ORP150 is up-regulated in tumors and, in breast tumors, may be associated with tumor invasiveness...
  28. ncbi request reprint Grb10/GrbIR as an in vivo substrate of Tec tyrosine kinase
    H Mano
    Department of Molecular Biology, Jichi Medical School, Tochigi, Japan
    Genes Cells 3:431-41. 1998
    ..To gain insights into the downstream pathways of Tec, we here looked for Tec-interacting proteins (TIPs) by using the yeast two-hybrid screening...
  29. ncbi request reprint An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity
    K Ozawa
    Biosignaling Department, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305 8566, Japan
    J Biol Chem 273:29262-71. 1998
    ..These results suggest that FGF-5S is a naturally expressed partial agonist/antagonist of FGF-5 neurotrophic activity in the brain and that its effects are exerted in part at the level of the receptor...
  30. ncbi request reprint Beta(2)-microglobulin identified as an apoptosis-inducing factor and its characterization
    M Mori
    Department of Hematology and the Division of Molecular Hematopoiesis, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Blood 94:2744-53. 1999
    ..We demonstrated that beta(2)m plays a previously unrecognized and important role in regulating the elimination of tumor cells, which occurs as a result of the action of beta(2)m as an apoptosis-inducing factor...
  31. ncbi request reprint SLUG, a ces-1-related zinc finger transcription factor gene with antiapoptotic activity, is a downstream target of the E2A-HLF oncoprotein
    T Inukai
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Mol Cell 4:343-52. 1999
    ....
  32. pmc Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase
    K Ohya
    Department of Molecular Biology, Jichi Medical School, Kawachi gun, Tochigi 329 0498, Japan
    Proc Natl Acad Sci U S A 96:11976-81. 1999
    ..BRDG1 thus appears to function as a docking protein acting downstream of Tec in BCR signaling...
  33. ncbi request reprint SNX5, a new member of the sorting nexin family, binds to the Fanconi anemia complementation group A protein
    T Otsuki
    Department of Hematology, Jichi Medical School, Yakushiji 3311 1, Minamikawachi, Kawachi, Tochigi, 329 0498, Japan
    Biochem Biophys Res Commun 265:630-5. 1999
    ..Further studies are needed to elucidate the functional significance of FANCA and SNX5 binding; however, we speculate that FANCA may affect SNX5 traffic with cell surface receptors...
  34. ncbi request reprint Mediation by the protein-tyrosine kinase Tec of signaling between the B cell antigen receptor and Dok-1
    K Yoshida
    Division of Functional Genomics, Departments of Hematology and Cardiology, Jichi Medical School, Kawachi gun, Tochigi 329 0498, Japan
    J Biol Chem 275:24945-52. 2000
    ..Furthermore, overexpression of Dok-1 inhibited activation of the c-fos promoter induced by stimulation of the BCR. These results suggest that Tec is an important mediator of signaling from the BCR to Dok-1...
  35. pmc Transcriptional regulation of the c-jun gene by retinoic acid and E1A during differentiation of F9 cells
    I Kitabayashi
    Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    EMBO J 11:167-75. 1992
    ..The downstream four motifs are located at 35 or 36 bp intervals in the same orientation. Substitution and insertion analysis indicates that these motifs and their regular intervals are important for the activity of the RERE...