Research Topics
Genomes and Genes | M OkabeSummaryAffiliation: Osaka University Country: Japan Publications
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Publications
'Green mice' as a source of ubiquitous green cellsM Okabe
Research Institute for Microbial Diseases, Osaka University, Suita, Japan
FEBS Lett 407:313-9. 1997..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...- Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertilityM Ikawa
Genome Information Research Center, Osaka University, 3 1 Yamadaoka, Suita, Osaka, 565 0871, Japan
Dev Biol 240:254-61. 2001..The data not only explain why the calmegin and fertilin beta knockout mouse lines share a common infertile phenotype, but also reveal the importance of the maturation of sperm membrane proteins in the endoplasmic reticulum... - Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitorsK Murayama
Department of Pathology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Oncogene 26:4882-8. 2007..The progenitor cell population increased consistently in 4OHT-treated transgenic mice. Our results show that PI3K/Akt signaling induces epidermal hyperplasia and proliferation of epidermal progenitors... - Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesisG Kondoh
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, Suita, Japan
FEBS Lett 458:299-303. 1999.... - Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cellsA Kawaguchi
Division of Neuroanatomy, Osaka University Graduate School of Medicine, Suita, Osaka, 565-0871, Japan
Mol Cell Neurosci 17:259-73. 2001..However, moderately EGFP-expressing cells in adults gained EGFP intensity when they formed neurospheres, suggesting embryonic and adult NSCs exist in different microenvironments in vivo... 
Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in miceK Horie
Collaborative Research Center for Advanced Science and Technology, and Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
Proc Natl Acad Sci U S A 98:9191-6. 2001..This system allows germ-line mutagenesis in vivo and will facilitate certain aspects of phenotype-driven genetic screening in mice...- A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animalsS Miyagawa
Division of Organ Transplantation, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565 0871, Japan
J Biochem 129:795-801. 2001..The sDAF showed a high level of expression in SEC and transgenic mice, suggesting that it will be useful in the development of transgenic pigs with high levels of expression... - Glowing podocytes in living mouse: transgenic mouse carrying a podocyte-specific promoterE Imai
First Department of Medicine, University School of Medicine, Osaka University, Osaka, Japan
Exp Nephrol 7:63-6. 1999.... - Enhanced immune responses in transgenic mice expressing a truncated form of the lymphocyte semaphorin CD100C Watanabe
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
J Immunol 167:4321-8. 2001..Collectively, these results not only indicate that CD100 has an important role in the immune system, but also that the soluble form of CD100 released from the cell surface can exert functions in vivo... - Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperoneH Tanaka
Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Suita City, Japan
Gene 204:159-63. 1997..Using the fluorescence in situ hybridization (FISH) technique, human Calmegin gene was mapped to chromosome 4q28.3-q31.1... - Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitationT Nakanishi
Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka, 565-0871, Japan
Dev Biol 237:222-31. 2001..3 media, but dissolved at pH 6.2. These data suggest that acrosomal alkalinization during incubation conducive for sperm capacitation may function to alter acrosomal contents and prepare them for release during the acrosome reaction... - Bone marrow is a reservoir of repopulating mesangial cells during glomerular remodelingT Ito
Department of Internal Medicine and Therapeutics, Osaka University School of Medicine, Osaka University, Suita, Osaka, Japan
J Am Soc Nephrol 12:2625-35. 2001..The results indicate that the bone marrow can give rise to mesangial cells in vivo... - Remodeling of the major pig xenoantigen by N-acetylglucosaminyltransferase III in transgenic pigS Miyagawa
Department of Regenerative Medicine, Osaka University Graduate School of Medicine, the Genome Information Research Center, Osaka University, Suita, Osaka 565 0871, Japan
J Biol Chem 276:39310-9. 2001.... - Tissue-dependent alteration of protease expression phenotype in murine peritoneal mast cells that were genetically labeled with green fluorescent proteinT Jippo
Department of Pathology, Osaka University Medical School, Suita, Japan
Am J Pathol 158:1695-701. 2001..The GFP(+) but Alcian blue(-) cells were considered as degranulated PMCS: The remarkable decrease or degranulation seemed to be necessary for the alteration of protease expression phenotype... - Mechanisms of sperm-egg interactions emerging from gene-manipulated animalsM Okabe
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka, 565 0871, Japan
Cell Mol Life Sci 64:1945-58. 2007..In this review, we use the results of gene manipulation experiments in animals to propose the basis for a new vision... - Cessation of spermatogenesis in juvenile spermatogonial depletion (jsd/jsd) miceY Kojima
Department of Urology, Osaka University Medical School, Suita, Japan
Int J Urol 4:500-7. 1997..Mice homozygous for the jsd (juvenile spermatogonial depletion) allele are sterile because they become azoospermic. The onset of such azoospermia was investigated by histologic analysis of sections of testes from jsd/jsd mice... - Activation of Akt signaling is sufficient to maintain pluripotency in mouse and primate embryonic stem cellsS Watanabe
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
Oncogene 25:2697-707. 2006..These results clearly demonstrate that Akt signaling sufficiently maintains pluripotency in mouse and primate ES cells, and support the notion that PI3K/Akt signaling axis regulates 'stemness' in a broad spectrum of stem cell systems... - Local nonpermissive and oriented permissive cues guide vestibular axons to the cerebellumY Tashiro
Laboratory of Neuroscience, Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
Development 128:973-81. 2001..Our observations suggest that local nonpermissive cues and oriented cues play a pivotal role in the guidance of vestibular axons to their central target... - Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptideH Hashimoto
Laboratories of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
J Neurochem 75:1810-7. 2000..We suggest that the mutant mice with markedly reduced PAC(1)R expression can serve as a useful animal model or cell culture system for further studies in PAC(1)R function... - The class IV semaphorin CD100 plays nonredundant roles in the immune system: defective B and T cell activation in CD100-deficient miceW Shi
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Osaka 565 0871, Japan
Immunity 13:633-42. 2000..These results demonstrate nonredundant and essential roles of CD100-CD72 interactions in the immune system... - Musashi and seven in absentia downregulate Tramtrack through distinct mechanisms in Drosophila eye developmentY Hirota
Division of Neuroanatomy D12, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka, Japan
Mech Dev 87:93-101. 1999..Other photoreceptor cells also failed to differentiate with abnormalities occurring late in the differentiation process. These results suggest that MSI and SINA function redundantly to downregulate TTK in developing photoreceptor cells... - The putative chaperone calmegin is required for sperm fertilityM Ikawa
Research Institute for Microbial Diseases, Osaka University, Suita, Japan
Nature 387:607-11. 1997..The defective zona pellucida-adhesion phenotype of sperm from calmegin-deficient mice is reminiscent of certain cases of unexplained infertility in human males... - Cyclin G1 is involved in G2/M arrest in response to DNA damage and in growth control after damage recoveryS H Kimura
Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
Oncogene 20:3290-300. 2001..Our results demonstrate that cyclin G1 plays roles in G2/M arrest, damage recovery and growth promotion after cellular stress... - Regulatory mechanisms of TRAF2-mediated signal transduction by Bcl10, a MALT lymphoma-associated proteinT Yoneda
Department of Anatomy, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
J Biol Chem 275:11114-20. 2000..The altered sensitivities to overexpressed Bcl10 may have been due to differences in signal responses to Bcl10 among cell types. Thus, Bcl10 was suggested to play crucial roles in the modulation of apoptosis associated with TRAF2... - Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin developmentM Tarutani
Department of Dermatology, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565, Japan
Proc Natl Acad Sci U S A 94:7400-5. 1997..Mutant mice died within a few days after birth, suggesting that Pig-a function is essential for proper skin differentiation and maintenance... - Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicityY Kitao
Department of Neuroanatomy, Kanazawa University Medical School, Kanazawa City, Ishikawa, Japan
J Clin Invest 108:1439-50. 2001..Our data underscore a possible role for ER stress in glutamate toxicity and pinpoint a key ER chaperone, ORP150, which contributes to the stress response critical for neuronal survival... - The Wilms' tumor gene WT1-GFP knock-in mouse reveals the dynamic regulation of WT1 expression in normal and leukemic hematopoiesisN Hosen
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA
Leukemia 21:1783-91. 2007.... - Development of efficient strategies for the production of genetically modified pigsH Nagashima
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University, Kawasaki, Japan
Theriogenology 59:95-106. 2003..It would be of great worth if cryopreserved clone embryos were available. We have demonstrated that cryopreservation of in vitro-produced porcine embryos as well as clone blastocysts is possible by our unique method... - The Drosophila secreted protein Argos regulates signal transduction in the Ras/MAPK pathwayK Sawamoto
Department of Molecular Neurobiology, University of Tsukuba, Ibaraki, Japan
Dev Biol 178:13-22. 1996..A phenotype of the sev; argos double mutants revealed that sev is epistatic to argos. These results provide evidence that Argos negatively regulates signal transduction events in the Ras/MAPK cascade... - Adenovirus serotype 35 vector-mediated transduction into human CD46-transgenic miceF Sakurai
Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka, Japan
Gene Ther 13:1118-26. 2006..However, the in vivo transduction efficiencies of Ad35 vectors in CD46TG mice are much lower than those of conventional Ad5 vectors in wild-type mice... - Signal transducer and activator of transcription 3 in the heart transduces not only a hypertrophic signal but a protective signal against doxorubicin-induced cardiomyopathyK Kunisada
Department of Molecular Medicine, Osaka University Medical School, Osaka 565 0871 Japan
Proc Natl Acad Sci U S A 97:315-9. 2000.... - Familial essential thrombocythemia associated with one-base deletion in the 5'-untranslated region of the thrombopoietin geneT Kondo
Third Department of Internal Medicine and the Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan
Blood 92:1091-6. 1998..Based on our findings, we propose that this region of the TPO gene may play a crucial role in regulating TPO expression. Our results strongly suggest that the identified mutation leads to familial ET... - Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros regionT Hara
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
Immunity 11:567-78. 1999..Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1 +CD45-cells were injected into neonatal liver of busulfan-treated mice. Thus, PCLP1 can be used to separate hemangioblasts that give rise to LTR-HSCs... - Spermatocyte-specific gene excision by targeted expression of Cre recombinaseH Ando
Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa, Japan
Biochem Biophys Res Commun 272:125-8. 2000..From these results, we concluded that this transgenic line possessing Pgk-2-driven expression of the Cre recombinase should be useful for identifying spermatogenic genes that function at or after the spermatocyte stage... - CIS3/SOCS3/SSI3 plays a negative regulatory role in STAT3 activation and intestinal inflammationA Suzuki
Institute of Life Science, Kurume University, Kurume 839-0861, Japan
J Exp Med 193:471-81. 2001..These data suggest that hyperactivation of STAT3 results in severe colitis and that CIS3 plays a negative regulatory role in intestinal inflammation by downregulating STAT3 activity... - ORP150 protects against hypoxia/ischemia-induced neuronal deathM Tamatani
Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka, Japan
Nat Med 7:317-23. 2001..These data indicate that ORP150 is an integral participant in ischemic cytoprotective pathways... - Suppression of STAT5 functions in liver, mammary glands, and T cells in cytokine-inducible SH2-containing protein 1 transgenic miceA Matsumoto
Institute of Life Science, Kurume University, Kurume 839 0861, Japan
Mol Cell Biol 19:6396-407. 1999..These data suggest that CIS1 functions as a specific negative regulator of STAT5 in vivo and plays an important regulatory role in the liver, mammary glands, and T cells... - Requirement of CD9 on the egg plasma membrane for fertilizationK Miyado
Institute of Life Science, Kurume University, Kurume, Fukuoka 839 0861, Japan
Science 287:321-4. 2000..When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion... - Involvement of distinct cellular compartments in the abnormal lymphoid organogenesis in lymphotoxin-alpha-deficient mice and alymphoplasia (aly) mice defined by the chimeric analysisM Matsumoto
First Department of Internal Medicine, School of Medicine, Ehime University, Japan
J Immunol 163:1584-91. 1999.... - Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G geneY N Harada
The Genome Research Group, National Institute of Radiological Sciences, Inage Ku, Chiba 263, Osaka 565, Japan
Mol Cell Biol 19:2366-72. 1999..Our in vitro studies showed that primary embryonic fibroblasts isolated from the xpg-deficient mice underwent premature senescence and exhibited the early onset of immortalization and accumulation of p53... - Nonrandom X chromosome inactivation in mouse embryos carrying Searle's T(X;16)16H translocation visualized using X-linked LACZ and GFP transgenesN Takagi
Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan
Cytogenet Genome Res 99:52-8. 2002..Although the HMG-LACZ transgene in H253 stock mice is an excellent marker of X chromosome inactivation, the present study suggests that it is infrequently de-repressed on the inactive X chromosome...