Hiroyuki Noji

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint [Nanoscience in rotary molecular motor proteins]
    Hiroyuki Noji
    Tanpakushitsu Kakusan Koso 52:297-300. 2007
  2. ncbi request reprint F1-ATPase changes its conformations upon phosphate release
    Tomoko Masaike
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226 8503, Japan
    J Biol Chem 277:21643-9. 2002
  3. ncbi request reprint Highly coupled ATP synthesis by F1-ATPase single molecules
    Yannick Rondelez
    LIMMS CNRS IIS, Tokyo 153 8505, Japan
    Nature 433:773-7. 2005
  4. ncbi request reprint [Single molecule study on the mechano-chemical coupling of F1-ATPase motor by the use of an ultra small reaction chamber]
    Hiroyuki Noji
    Tanpakushitsu Kakusan Koso 52:302-8. 2007
  5. doi request reprint Highly sensitive restriction enzyme assay and analysis: a review
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Osaka 567 0047, Japan
    Anal Bioanal Chem 391:2423-32. 2008
  6. pmc Single molecule energetics of F1-ATPase motor
    Eiro Muneyuki
    Department of Physics, Faculty of Science and Technology, Chuo University, Tokyo, Japan
    Biophys J 92:1806-12. 2007
  7. ncbi request reprint Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation
    Kengo Adachi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku ku, Tokyo 169 8555, Japan
    Cell 130:309-21. 2007
  8. ncbi request reprint An integrated system for enzymatic cleavage and electrostretching of freely-suspended single DNA molecules
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, 8 1, Ibaraki, 567 0047, Japan
    Lab Chip 7:1738-45. 2007
  9. pmc Temperature-sensitive reaction intermediate of F1-ATPase
    Rikiya Watanabe
    Department of Mechanical Engineering, University of Tokyo, Tokyo 113 8656, Japan
    EMBO Rep 9:84-90. 2008
  10. doi request reprint Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamber
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
    Biomed Microdevices 10:539-46. 2008

Collaborators

Detail Information

Publications26

  1. ncbi request reprint [Nanoscience in rotary molecular motor proteins]
    Hiroyuki Noji
    Tanpakushitsu Kakusan Koso 52:297-300. 2007
  2. ncbi request reprint F1-ATPase changes its conformations upon phosphate release
    Tomoko Masaike
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226 8503, Japan
    J Biol Chem 277:21643-9. 2002
    ..This is the first evidence that the beta subunit changes its conformation upon phosphate release, which may share a common mechanism of exerting motility with other motor proteins...
  3. ncbi request reprint Highly coupled ATP synthesis by F1-ATPase single molecules
    Yannick Rondelez
    LIMMS CNRS IIS, Tokyo 153 8505, Japan
    Nature 433:773-7. 2005
    ..We provide here direct evidence that F1 is designed to tightly couple its catalytic reactions with the mechanical rotation. Our results suggest that the epsilon-subunit has an essential function during ATP synthesis...
  4. ncbi request reprint [Single molecule study on the mechano-chemical coupling of F1-ATPase motor by the use of an ultra small reaction chamber]
    Hiroyuki Noji
    Tanpakushitsu Kakusan Koso 52:302-8. 2007
  5. doi request reprint Highly sensitive restriction enzyme assay and analysis: a review
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Osaka 567 0047, Japan
    Anal Bioanal Chem 391:2423-32. 2008
    ..Finally, we take a brief look at the general trends in technological development in this field by comparing the advantages and disadvantages of performing assays at bulk, microscale and single-molecule levels...
  6. pmc Single molecule energetics of F1-ATPase motor
    Eiro Muneyuki
    Department of Physics, Faculty of Science and Technology, Chuo University, Tokyo, Japan
    Biophys J 92:1806-12. 2007
    ..The results imply that microscopically defined work at a single molecule level cannot be directly compared with macroscopically defined free energy input...
  7. ncbi request reprint Coupling of rotation and catalysis in F(1)-ATPase revealed by single-molecule imaging and manipulation
    Kengo Adachi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku ku, Tokyo 169 8555, Japan
    Cell 130:309-21. 2007
    ..This and other results suggest that the affinity for ADP also decreases with rotation, and thus ADP release contributes part of energy for rotation. Together with previous results, the coupling scheme is now basically complete...
  8. ncbi request reprint An integrated system for enzymatic cleavage and electrostretching of freely-suspended single DNA molecules
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, 8 1, Ibaraki, 567 0047, Japan
    Lab Chip 7:1738-45. 2007
    ..Furthermore, with the employment of porous polyacrylamide gel, the effective manipulation of DNA assays and the ability to combine conventionally independent bioanalytical processes have been demonstrated...
  9. pmc Temperature-sensitive reaction intermediate of F1-ATPase
    Rikiya Watanabe
    Department of Mechanical Engineering, University of Tokyo, Tokyo 113 8656, Japan
    EMBO Rep 9:84-90. 2008
    ..Conversely, the addition of inorganic phosphate caused a pause at an angle of +80 degrees from that of the intermediate state. These observations strongly suggest that the newly found reaction intermediate is an ADP-releasing step...
  10. doi request reprint Loop-mediated isothermal amplification of a single DNA molecule in polyacrylamide gel-based microchamber
    Liza Lam
    The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
    Biomed Microdevices 10:539-46. 2008
    ....
  11. ncbi request reprint Origin of apparent negative cooperativity of F(1)-ATPase
    Sakurako Ono
    Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, 226 8503 Yokohama, Japan
    Biochim Biophys Acta 1607:35-44. 2003
    ..Thus, the frequently observed K(m)'s of 100-300 microM and 1-30 microM range for wild-type F(1)-ATPase correspond to ATP binding to a noncatalytic site and catalytic site, respectively...
  12. ncbi request reprint Mechanically driven ATP synthesis by F1-ATPase
    Hiroyasu Itoh
    Tsukuba Research Laboratory, Hamamatsu Photonics KK, Joko, Hamamatsu 431 3103, Japan
    Nature 427:465-8. 2004
    ..This shows that a vectorial force (torque) working at one particular point on a protein machine can influence a chemical reaction occurring in physically remote catalytic sites, driving the reaction far from equilibrium...
  13. ncbi request reprint Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation
    Takayuki Nishizaka
    Kansai Advanced Research Center, Protein Biophysics Group, Iwaoka 588 2, Nishi ku, Kobe 651 2492, Japan
    Nat Struct Mol Biol 11:142-8. 2004
    ....
  14. ncbi request reprint Stepping rotation of F(1)-ATPase with one, two, or three altered catalytic sites that bind ATP only slowly
    Takayuki Ariga
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226 8503, Japan
    J Biol Chem 277:24870-4. 2002
    ....
  15. ncbi request reprint [Methods for visualization of a single molecule of rotating molecular motor in living organisms]
    Yoko Hara
    Nihon Seirigaku Zasshi 65:179-84. 2003
  16. ncbi request reprint [Molecular mechanism of torque generation in F1-motor]
    Hiroyuki Noji
    Tanpakushitsu Kakusan Koso 47:1174-81. 2002
  17. ncbi request reprint Thermally responsive supramolecular nanomeshes for on/off switching of the rotary motion of F1-ATPase at the single-molecule level
    Satoshi Yamaguchi
    PRESTO Synthesis and Control, JST, Kyoto University, Katsura, Nishikyo ku, Kyoto, 615 8510, Japan
    Chemistry 14:1891-6. 2008
    ..The integration of biomolecules and hard materials, interfaced with intelligent soft materials such as supramolecular hydrogels, is promising for the development of novel semi-synthetic nano-biodevices...
  18. ncbi request reprint Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve)
    Kyoko Atsuta
    Center for International Research on Micro Mechatronics CIRMM, Institute of Industrial Science, University of Tokyo, 4 6 1 Komaba, Meguro ku, Tokyo 153 8505, Japan
    Lab Chip 4:333-6. 2004
    ..Selective patterning with three kinds of fluorescent micro beads indicated the possibility of patterning of different proteins on the same substrate by using the sieve...
  19. doi request reprint Photo gel-sol/sol-gel transition and its patterning of a supramolecular hydrogel as stimuli-responsive biomaterials
    Shinji Matsumoto
    Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Nishikyo ku, Kyoto, 615 8510, Japan
    Chemistry 14:3977-86. 2008
    ..These results demonstrated that the photoresponsive supramolecular hydrogel and the resulting nanomeshes may provide unique biomaterials for the spatiotemporal manipulation of various biomolecules and live bacteria...
  20. pmc Evidence for rotation of V1-ATPase
    Hiromi Imamura
    ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, 5800 3 Nagatsuta, Midori ku, Yokohama 226 0026, Japan
    Proc Natl Acad Sci U S A 100:2312-5. 2003
    ..This study provides experimental evidence that V(o)V(1)-ATPase is a rotary motor and that both D and F subunits constitute a rotor shaft...
  21. ncbi request reprint Single-molecule imaging of rotation of F1-ATPase
    Kengo Adachi
    Center for Integrative Bioscience, Okazaki National Research Institutes, Okazaki, Aichi 444 8585, Japan
    Methods Enzymol 361:211-27. 2003
  22. pmc The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer
    Ryohei Yasuda
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 100:9314-8. 2003
    ....
  23. ncbi request reprint Visualization of RecA filaments and DNA by fluorescence microscopy
    Taro Nishinaka
    Yashima Super structured Helix Project, ERATO, Japan Science and Technology Agency, 101 Creation Core Nagoya, 2266 22 Anagahora, Nagoya 463 0003, Japan
    J Biochem 141:147-56. 2007
    ..We showed that the RecA-DNA filament disassembled in the direction from 5' to 3' of ssDNA as dATP hydrolysis proceeded...
  24. ncbi request reprint Chemomechanical coupling in single-molecule F-type ATP synthase
    Ryota Iino
    The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
    J Bioenerg Biomembr 37:451-4. 2005
    ..It is plausible that the conformational change of epsilon switches the catalytic mode of F(0)F(1) for highly coupled ATP synthesis...
  25. ncbi request reprint Microfabricated arrays of femtoliter chambers allow single molecule enzymology
    Yannick Rondelez
    Nat Biotechnol 23:361-5. 2005
    ..The approach should be of interest for many ultrasensitive bioassays at the single-molecule level...
  26. pmc Activation of pausing F1 motor by external force
    Yoko Hirono-Hara
    Institute of Industrial Science and Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, University of Tokyo, Tokyo 153 8505, Japan
    Proc Natl Acad Sci U S A 102:4288-93. 2005
    ..Furthermore, the mechanical work for the forced rotation was efficiently converted into work for expelling ADP from the catalytic site, supporting the tight coupling between the rotation and catalytic event...