Toru Nakano

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint Orderly and nonstochastic acquisition of CD94/NKG2 receptors by developing NK cells derived from embryonic stem cells in vitro
    Rebecca H Lian
    Terry Fox Laboratory, British Columbia Cancer Agency, University of British Columbia, 601 West 10th Avenue, Vancouver, British Columbia, Canada V5Z 1L3
    J Immunol 168:4980-7. 2002
  2. ncbi request reprint Hematopoietic stem cells: generation and manipulation
    Toru Nakano
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
    Trends Immunol 24:589-94. 2003
  3. ncbi request reprint [Overview: signals controlling stem cell systems]
    Toru Nakano
    Tanpakushitsu Kakusan Koso 49:699-702. 2004
  4. ncbi request reprint Developing our understanding of the hematopoietic system
    T Nakano
    Department of Molecular Cell Biology, Osaka University, Japan
    Int J Hematol 71:294-6. 2000
  5. ncbi request reprint Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes
    Takuji Yoshimura
    Supply Center of Inbred Animals of Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Dev Biol 335:216-27. 2009
  6. ncbi request reprint Redirecting differentiation of hematopoietic progenitors by a transcription factor, GATA-2
    Kenji Kitajima
    Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    Blood 107:1857-63. 2006
  7. ncbi request reprint Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation
    Daijiro Sugiyama
    Department of Stem Cell Pathology, Medical School, Osaka University, Osaka, Japan
    Blood 111:1924-32. 2008
  8. doi request reprint Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells
    Kanako Kojima
    Department of Pathology, Medical School, Osaka University, Osaka 565 0871, Japan
    Genes Cells 14:1155-65. 2009
  9. pmc MVH in piRNA processing and gene silencing of retrotransposons
    Satomi Kuramochi-Miyagawa
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    Genes Dev 24:887-92. 2010
  10. doi request reprint PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos
    Toshinobu Nakamura
    Department of Pathology, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Nature 486:415-9. 2012

Collaborators

Detail Information

Publications99

  1. ncbi request reprint Orderly and nonstochastic acquisition of CD94/NKG2 receptors by developing NK cells derived from embryonic stem cells in vitro
    Rebecca H Lian
    Terry Fox Laboratory, British Columbia Cancer Agency, University of British Columbia, 601 West 10th Avenue, Vancouver, British Columbia, Canada V5Z 1L3
    J Immunol 168:4980-7. 2002
    ....
  2. ncbi request reprint Hematopoietic stem cells: generation and manipulation
    Toru Nakano
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
    Trends Immunol 24:589-94. 2003
    ..The generation of blood cells from embryonic stem cells and the manipulation of HSCs continue to provide insights into other stem cell systems. The importance of HSCs as a model of an ideal source for cell therapy is increasing...
  3. ncbi request reprint [Overview: signals controlling stem cell systems]
    Toru Nakano
    Tanpakushitsu Kakusan Koso 49:699-702. 2004
  4. ncbi request reprint Developing our understanding of the hematopoietic system
    T Nakano
    Department of Molecular Cell Biology, Osaka University, Japan
    Int J Hematol 71:294-6. 2000
    ..The symposium entitled "Developing Our Understanding of the Hematopoietic System" was held in the 61st annual meeting of the Japanese Society of Hematology. Six speakers presented their interesting original study...
  5. ncbi request reprint Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes
    Takuji Yoshimura
    Supply Center of Inbred Animals of Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Dev Biol 335:216-27. 2009
    ..These observations indicate that Gtsf1/Cue110 is required for spermatogenesis and involved in retrotransposon suppression in male germ cells...
  6. ncbi request reprint Redirecting differentiation of hematopoietic progenitors by a transcription factor, GATA-2
    Kenji Kitajima
    Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    Blood 107:1857-63. 2006
    ..1 promoter region. This study demonstrates that the function of GATA-2 is modified in a context-dependent manner by expression of PU.1, which in turn is regulated by GATA-2...
  7. ncbi request reprint Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation
    Daijiro Sugiyama
    Department of Stem Cell Pathology, Medical School, Osaka University, Osaka, Japan
    Blood 111:1924-32. 2008
    ..These results indicate that FOG-1 inhibits mast-cell differentiation in a differentiation stage-dependent manner, and its effects are produced via different molecular mechanisms...
  8. doi request reprint Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells
    Kanako Kojima
    Department of Pathology, Medical School, Osaka University, Osaka 565 0871, Japan
    Genes Cells 14:1155-65. 2009
    ..These data suggest that the formation of complexes between MILI, MIWI and TDRD1/MTR-1 is critical for the integrated subcellular localizations of these proteins, and is presumably essential for spermatogenesis...
  9. pmc MVH in piRNA processing and gene silencing of retrotransposons
    Satomi Kuramochi-Miyagawa
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    Genes Dev 24:887-92. 2010
    ..Comprehensive analysis of piRNAs in MVH-deficient fetal male germ cells showed that MVH plays crucial roles in the early phase of the ping-pong amplification cycle...
  10. doi request reprint PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos
    Toshinobu Nakamura
    Department of Pathology, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Nature 486:415-9. 2012
    ..This type of regulatory mechanism could be involved in DNA modifications in somatic cells as well as in early embryos...
  11. ncbi request reprint Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-3
    Hiroki Umehara
    Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamada oka, Suita, Osaka, Japan
    Stem Cells 25:2705-11. 2007
    ..In contrast, Akt signaling activation enhanced the growth of ICM but did not increase the efficiency of ES cell derivation. Our study establishes an efficient means for ES cell derivation by pharmacological inhibition of GSK-3...
  12. pmc DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes
    Satomi Kuramochi-Miyagawa
    Department of Pathology, Medical School, Graduate School of Frontier Biosciences, Research Institute for Microbial Diseases, Osaka University, Yamada oka 2 2 Suita, Osaka 565 0871, Japan
    Genes Dev 22:908-17. 2008
    ..Our data strongly suggest that MILI and MIWI2 play essential roles in establishing de novo DNA methylation of retrotransposons in fetal male germ cells...
  13. doi request reprint AKT signaling promotes derivation of embryonic germ cells from primordial germ cells
    Tohru Kimura
    Department of Pathology, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka, Japan
    Development 135:869-79. 2008
    ..The p53 deficiency, but not GSK3 inhibition, recapitulated the effects of AKT hyperactivation on EG cell derivation, suggesting that p53 is one of the crucial downstream targets of the PI3K/AKT signal and that GSK3 is not...
  14. doi request reprint Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation
    Noriko Yamano
    Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 392:311-6. 2010
    ..These results indicate that Akt signaling promotes a novel metastable and pluripotent state that is intermediate to those of ESCs and PGCs...
  15. ncbi request reprint PGC7/Stella protects against DNA demethylation in early embryogenesis
    Toshinobu Nakamura
    Department of Pathology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    Nat Cell Biol 9:64-71. 2007
    ..These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization...
  16. pmc Multipotential differentiation ability of GATA-1-null erythroid-committed cells
    Kenji Kitajima
    Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Genes Dev 20:654-9. 2006
    ..Our data suggest that GATA-1 is a critical transcription factor to fix erythroid progenitors to the erythroid lineage...
  17. ncbi request reprint Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes
    Yoshiaki Okada
    Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Blood 104:2027-34. 2004
    ..Thus, these studies demonstrate that the novel TME binding transcription factors, USF1 and 2, transactivate rat and human PF4 promoters and may play an important role in megakaryocytic gene expression...
  18. pmc GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells
    Yusuke Shiromoto
    Department of Pathology, Medical School, 2Graduate School of Frontier Biosciences, Osaka University, Yamada oka 2 2 Suita, Osaka 565 0871, Japan
    RNA 19:803-10. 2013
    ..GPAT2 is not only one of the MILI bound proteins but also a protein essential for primary piRNA biogenesis...
  19. ncbi request reprint Enhanced insulin sensitivity, energy expenditure and thermogenesis in adipose-specific Pten suppression in mice
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Graduate School of Frontier Bioscience, Osaka University, 2 2 Yamadaoka, Suita, Osaka, Japan
    Nat Med 10:1208-15. 2004
    ..Our results suggest that altered expression of adipose Pten could regulate insulin sensitivity and energy expenditure. Suppression of adipose Pten may become a beneficial strategy to treat type 2 diabetes and obesity...
  20. ncbi request reprint Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells
    Jie Zheng
    Department of Pathology, Medical School and Graduate School of Frontier Biosciences, Osaka University, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    Blood 107:520-7. 2006
    ..Thus, GATA-1 affects the proliferation and differentiation of erythrocytes by different mechanisms...
  21. doi request reprint Increase of hematopoietic progenitor and suppression of endothelial gene expression by Runx1 expression during in vitro ES differentiation
    Eiko Sakai
    Department of Pathology, School of Medicine and Frontier Biosciences, Osaka University, Yamadaok, Osaka, Japan
    Exp Hematol 37:334-45. 2009
    ..To reveal the roles of Runx1, we examined how and when Runx1 functions during development of hematopoiesis, and revealed the genes controlled by Runx1...
  22. doi request reprint Effects of dppa3 on DNA methylation dynamics during primordial germ cell development in mice
    Hiroyuki Nakashima
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    Biol Reprod 88:125. 2013
    ..From our findings, we propose that Dppa3 is involved in the Tet-mediated active demethylation process during reprogramming of PGCs...
  23. ncbi request reprint Conditional loss of PTEN leads to testicular teratoma and enhances embryonic germ cell production
    Tohru Kimura
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Development 130:1691-700. 2003
    ..PTEN appears to be essential for germ cell differentiation and an important factor in testicular germ cell tumor formation...
  24. doi request reprint Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways
    Daisuke Ikegami
    Departments of Bone and Cartilage Biology, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka, Japan
    Development 138:1507-19. 2011
    ..Thus, continued expression of Sox9 in differentiated chondrocytes is essential for subsequent hypertrophy and sustains chondrocyte-specific survival mechanisms by binding to the Pik3ca promoter, inducing Akt phosphorylation...
  25. ncbi request reprint Reduced expression of IL-12 receptor beta2 and IL-18 receptor alpha genes in natural killer cells and macrophages derived from B6-mi/mi mice
    Tatsuki R Kataoka
    Department of Pathology, Medical School Graduate School of Frontier Bioscience, Osaka University, Osaka, Japan
    Lab Invest 85:146-53. 2005
    ..The abnormal NK cell and macrophage of B6-mi/mi mice appear to be due to decreased expression of the IL-12Rbeta2 and IL-18Ralpha genes...
  26. ncbi request reprint DNA methylation in mouse testes
    Satomi Kuramochi-Miyagawa
    Department of Pathology, Medical School, Osaka University, Osaka, Japan
    Methods Mol Biol 1093:97-109. 2014
    ..Here, we describe two methods, bisulfite sequencing and Southern blotting using a methylation-sensitive restriction enzyme, for analysis of DNA methylation of LINE1, IAP, and imprinted genes in mouse testes...
  27. pmc Multiple ETS family proteins regulate PF4 gene expression by binding to the same ETS binding site
    Yoshiaki Okada
    Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    PLoS ONE 6:e24837. 2011
    ..Together, these data suggest that FLI-1, ELF-1, and GABP regulate PF4 gene expression through the -51 ETS site in megakaryocytes and implicate the differentiation stage-specific regulation of PF4 gene expression by multiple ETS factors...
  28. ncbi request reprint In vitro differentiation of mouse embryonic stem cells to hematopoietic cells on an OP9 stromal cell monolayer
    Kenji Kitajima
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka, Osaka 565 0871, Japan
    Methods Enzymol 365:72-83. 2003
  29. ncbi request reprint The stabilization of beta-catenin leads to impaired primordial germ cell development via aberrant cell cycle progression
    Tohru Kimura
    Department of Pathology, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Dev Biol 300:545-53. 2006
    ..Our results show that the suppression of Wnt/beta-catenin signaling is a prerequisite for the normal development of PGCs...
  30. ncbi request reprint Differentiation status dependent function of FOG-1
    Makoto Tanaka
    Department of Pathology, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Genes Cells 9:1213-26. 2004
    ..These results suggest that FOG-1 functions in a cell differentiation context-dependent manner...
  31. ncbi request reprint Induction of pluripotency in primordial germ cells
    Tohru Kimura
    Department of Pathology, Medical School, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    Histol Histopathol 26:643-50. 2011
    ..The regulatory mechanisms of PGC dedifferentiation are discussed in comparison with those involved in the dedifferentiation of testicular stem cells, ESC pluripotency, and somatic nuclear reprogramming...
  32. doi request reprint PI3K/Akt signaling as a key regulatory pathway for chondrocyte terminal differentiation
    Keisuke Kita
    Department of Pathology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Genes Cells 13:839-50. 2008
    ..These findings demonstrate that PI3K/Akt signaling is a key regulator in terminal chondrocyte differentiation in both embryonic and adult chondrogenesis...
  33. ncbi request reprint Involvement of connective tissue-type mast cells in Th1 immune responses via Stat4 expression
    Tatsuki R Kataoka
    Department of Pathology, Osaka University Medical School, Yamada oka 2 2, Suita, Osaka, 565 0871, Japan
    Blood 105:1016-20. 2005
    ..These data indicate that expression of Stat4 in CTMCs plays an important role on Th1 immune responses...
  34. ncbi request reprint Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less
    Masaaki Masuhara
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 308:927-32. 2003
    ..In addition, the amount of p53 was increased and transactivation by p53 was enhanced by mGCL-1. Thus, mGCL-1 turned out to be a factor modulating MDM2-p53 axis by enhanced degradation of MDM2...
  35. ncbi request reprint Tumorigenesis facilitated by Pten deficiency in the skin: evidence of p53-Pten complex formation on the initiation phase
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Osaka University, Suita, 565 0871, Japan
    Cancer Sci 95:639-43. 2004
    ..These observations suggest that Pten is involved in rapid recruitment of p53 in the tumor initiation phase...
  36. ncbi request reprint Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
    Satomi Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    Development 131:839-49. 2004
    ..These data indicate that MILI is essential for the differentiation of spermatocytes...
  37. doi request reprint Effects of Akt signaling on nuclear reprogramming
    Toshinobu Nakamura
    Graduate School of Frontier Bioscience, Osaka University, Suita, Osaka, Japan
    Genes Cells 13:1269-77. 2008
    ..In contrast, Akt signaling significantly reduced the efficiency of reprogramming with nuclear transfer. Our results demonstrate that Akt signaling plays important roles on the nuclear reprogramming of somatic cells...
  38. doi request reprint Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis
    Yu Jung Liu
    Graduate School of Frontier Biosciences, Osaka University, Japan
    Biol Reprod 86:40. 2012
    ..These findings demonstrate that DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation...
  39. doi request reprint A forward genetic screen to study mammalian RNA interference: essential role of RNase IIIa domain of Dicer1 in 3' strand cleavage of dsRNA in vivo
    Kazuhito Ohishi
    Department of Pathology, Medical School, Osaka University, Japan
    FEBS J 279:832-43. 2012
    ....
  40. ncbi request reprint [Establishment of epigenetic asymmetry in the zygote]
    Toshinobu Nakamura
    Tanpakushitsu Kakusan Koso 52:434-40. 2007
  41. ncbi request reprint [Analysis of hematopoietic differentiation using ES cell in vitro differentiation induction system]
    Kenji Kitajima
    Tanpakushitsu Kakusan Koso 48:223-31. 2003
  42. ncbi request reprint Unique alignment and texture of biological apatite crystallites in typical calcified tissues analyzed by microbeam X-ray diffractometer system
    T Nakano
    Department of Materials Science and Engineering, Graduate School of Engineering, Osaka University, Suita, Japan
    Bone 31:479-87. 2002
    ..It was concluded that the microscale measurement of biological Ap texture is one of the useful new methods for evaluating mechanical function and stress distribution in vivo in calcified tissues...
  43. ncbi request reprint Molecular cloning and genomic organization of mouse homologue of Drosophila germ cell-less and its expression in germ lineage cells
    T Kimura
    Department of Molecular Cell Biology, Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3 1, Yamada oka, Suita Shi, Osaka, 565 0871, Japan
    Biochem Biophys Res Commun 262:223-30. 1999
    ..Further studies including gene disruption analysis would provide an important insight into mammalian germ lineage development...
  44. ncbi request reprint Two mouse piwi-related genes: miwi and mili
    S Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Mech Dev 108:121-33. 2001
    ..MIWI may be involved in RNA processing or translational regulation, since MIWI was found to possess RNA binding activity. Our data suggest that miwi and mili regulate spermatogenesis and primordial germ cell production...
  45. ncbi request reprint Different roles of glycosylphosphatidylinositol in various hematopoietic cells as revealed by a mouse model of paroxysmal nocturnal hemoglobinuria
    Y Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Blood 94:2963-70. 1999
    ....
  46. pmc Coloration using higher order optical interference in the wing pattern of the Madagascan sunset moth
    S Yoshioka
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    J R Soc Interface 5:457-64. 2008
    ..The physical mechanism of the colour pattern formation is briefly discussed with the several mathematical models proposed so far...
  47. ncbi request reprint [Establishment, maintenance and differentiation induction of embryonic stem cells]
    Toru Nakano
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University
    Nihon Rinsho 61:385-9. 2003
    ..Establishment, maintenance and in vitro differentiation of the cells are key points for the utilization of ES cells in regeneration medicine...
  48. doi request reprint Aberrant expression of disintegrin-metalloprotease proteins in the formation and progression of uterine cervical cancer
    Mohammed Shaker
    Department of Molecular Pathology, Graduate School of Medicine, Osaka University, Japan
    Pathobiology 78:149-61. 2011
    ..However, little is known about the expression of ADAMs and ADAMTSs in the development of human cervical cancer...
  49. pmc Critical roles of Pten in B cell homeostasis and immunoglobulin class switch recombination
    Akira Suzuki
    Department of Molecular Cell Biology, Research Institute for Microbial Disease, Osaka University, Osaka 565 0871, Japan
    J Exp Med 197:657-67. 2003
    ..Thus, Pten plays a role in developmental fate determination of B cells and is an indispensable regulator of B cell homeostasis...
  50. ncbi request reprint Identification of PGC7, a new gene expressed specifically in preimplantation embryos and germ cells
    Masatake Sato
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka Suita 565 0871, Japan
    Mech Dev 113:91-4. 2002
    ..Immunohistochemical analysis revealed that PGC7 was specifically expressed in early pre-implantation embryos, PGCs and oocytes. These results suggest that PGC7 might play an important role in the development of PGCs and oocytes...
  51. pmc Mouse germ cell-less as an essential component for nuclear integrity
    Tohru Kimura
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita Shi, Osaka 565 0871, Japan
    Mol Cell Biol 23:1304-15. 2003
    ....
  52. ncbi request reprint Development of hematopoietic cells from embryonic stem cells
    A Suzuki
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Int J Hematol 73:1-5. 2001
    ..The usefulness and limits of embryonic stem cell-derived hematopoietic cells in cell therapy are also discussed...
  53. ncbi request reprint Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors
    K Murayama
    Department of Pathology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Oncogene 26:4882-8. 2007
    ..The progenitor cell population increased consistently in 4OHT-treated transgenic mice. Our results show that PI3K/Akt signaling induces epidermal hyperplasia and proliferation of epidermal progenitors...
  54. ncbi request reprint Cadherin-mediated cell interaction regulates germ cell determination in mice
    Daiji Okamura
    Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840, Murodo cho, Izumi, Osaka, 594 1101, Japan
    Development 130:6423-30. 2003
    ..These results showed that E-cadherin-mediated cell-cell interaction among cells containing PGC precursors is essential to directing such cells to the germ cell fate...
  55. pmc GATA-2 and GATA-2/ER display opposing activities in the development and differentiation of blood progenitors
    Kenji Kitajima
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita 565 0871, Japan
    EMBO J 21:3060-9. 2002
    ....
  56. ncbi request reprint Embryonic stem cell
    M Sato
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita
    Intern Med 40:195-200. 2001
    ....
  57. ncbi request reprint Alteration of cell adhesion and cell cycle properties of ES cells by an inducible dominant interfering Myb mutant
    N Iwai
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita 565-0871, Osaka, Japan
    Oncogene 20:1425-34. 2001
    ..These results are well consistent with the recent report on the phenotype of B-Myb null mice and show that the regulation of cell adhesion is an important B-Myb function that has not yet been assumed...
  58. ncbi request reprint T cell-specific loss of Pten leads to defects in central and peripheral tolerance
    A Suzuki
    Department of Molecular and Cellular Biology, Osaka University, 565 0871, Osaka, Japan
    Immunity 14:523-34. 2001
    ..Peripheral tolerance to SEB is also impaired in Pten(flox/-) mice. PTEN is thus an important regulator of T cell homeostasis and self-tolerance...
  59. ncbi request reprint Hypersensitivity of megakaryocyte progenitors to thrombopoietin in essential thrombocythemia
    H Kawasaki
    Department of Pediatrics, Kansai Medical University, Moriguchi, Osaka, Japan
    Am J Hematol 68:194-7. 2001
    ..We observed hypersensitivity of megakaryocyte progenitors to TPO in a child with ET. Our results suggest that spontaneous CFU-Meg formation in patients with ET may be due to hypersensitivity to TPO...
  60. ncbi request reprint In vitro proliferation potential of AC133 positive cells in peripheral blood
    K Matsumoto
    Osaka Red Cross Blood Center, Osaka, Japan
    Stem Cells 18:196-203. 2000
    ..These findings suggest that to isolate primitive hematopoietic cells from steady-state PB, selection by AC133 expression is better than selection by CD34 expression...
  61. ncbi request reprint In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis
    T Nakano
    Department of Molecular Cell Biology, Osaka University, Japan
    Int J Hematol 65:1-8. 1996
    ..The OP9 system should facilitate to elucidate the molecular mechanisms of hematopoietic system development and hematopoietic cell differentiation...
  62. ncbi request reprint Role of protein kinase C in U46619-induced platelet shape change, aggregation and secretion
    T Nakano
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    Thromb Res 56:299-306. 1989
    ....
  63. ncbi request reprint Vascular smooth muscle cell-derived, Gla-containing growth-potentiating factor for Ca(2+)-mobilizing growth factors
    T Nakano
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    J Biol Chem 270:5702-5. 1995
    ..The presence of a specific binding site suggests that the effect of GPF is mediated by a receptor. Thus, GPF may be a new type of extracellular factor regulating VSMC proliferation...
  64. ncbi request reprint Retinol induces platelet aggregation via activation of phospholipase A2
    T Nakano
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    Biochem Biophys Res Commun 154:1075-80. 1988
    ..Moreover, we discussed that the arachidonic acid release may be mediated by the action of phospholipase A2...
  65. ncbi request reprint Different effects of two thromboxane A2/prostaglandin H2 receptor ligands, U46619 and S-145, on rabbit platelets
    T Nakano
    Shionogi Research Laboratories, Shionogi and Co, Ltd, Osaka, Japan
    FEBS Lett 234:309-12. 1988
    ..These results suggest an essential role of phospholipase C in the induction of platelet aggregation and secretion and that some factor other than Ca2+ and phospholipase C participates in platelet shape change...
  66. ncbi request reprint Activation of Akt signaling is sufficient to maintain pluripotency in mouse and primate embryonic stem cells
    S Watanabe
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    Oncogene 25:2697-707. 2006
    ..These results clearly demonstrate that Akt signaling sufficiently maintains pluripotency in mouse and primate ES cells, and support the notion that PI3K/Akt signaling axis regulates 'stemness' in a broad spectrum of stem cell systems...
  67. pmc Hepatocyte-specific Pten deficiency results in steatohepatitis and hepatocellular carcinomas
    Yasuo Horie
    Department of Gastroenterology, Akita University School of Medicine, Akita, Japan
    J Clin Invest 113:1774-83. 2004
    ..Pten is thus an important regulator of lipogenesis, glucose metabolism, hepatocyte homeostasis, and tumorigenesis in the liver...
  68. ncbi request reprint Efficient and repetitive production of hematopoietic and endothelial cells from feeder-free monolayer culture system of primate embryonic stem cells
    Hong Zhang
    Department of Hematology, Research Institute, International Medical Center of Japan, Tokyo 162 8655, Japan
    Biol Reprod 74:295-306. 2006
    ..Our method provides a highly controllable culture protocol for repetitive production of hematopoietic and endothelial cells from feeder-free monolayer cultivation of primate ES cells...
  69. ncbi request reprint Pluripotential competence of cells associated with Nanog activity
    Shin Ya Hatano
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Mech Dev 122:67-79. 2005
    ..Thus, the activity of Nanog is tightly correlated with an undifferentiated state of cells even in nuclear reprogrammed somatic cells. Nanog may function as a key regulator for sustaining pluripotency in a dose-dependent manner...
  70. ncbi request reprint Keratinocyte-specific Pten deficiency results in epidermal hyperplasia, accelerated hair follicle morphogenesis and tumor formation
    Akira Suzuki
    Department of Biochemistry, Akita University School of Medicine, Akita 010 8543, Japan
    Cancer Res 63:674-81. 2003
    ..Pten is thus an important regulator of normal development and oncogenesis in the skin...
  71. pmc The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis
    Koichi Hamada
    Department of Molecular Biology, Department of Microbiology, Department of Gastroenterology, Akita University School of Medicine, Akita 010 8543, Japan
    Genes Dev 19:2054-65. 2005
    ..Pten is thus indispensable for normal cardiovascular morphogenesis and post-natal angiogenesis, including tumor angiogenesis...
  72. ncbi request reprint Notch-RBP-J signaling is involved in cell fate determination of marginal zone B cells
    Kenji Tanigaki
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto, 606 8501, Japan
    Nat Immunol 3:443-50. 2002
    ..In contrast, these mice exhibited increased mortality rates after blood-borne bacterial infection, which indicates that MZB cells play pivotal roles in the clearance of these bacteria...
  73. pmc Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling
    Koji Eto
    Departments of Cell Biology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:12819-24. 2002
    ..01). These results establish that ES cells are a ready source of mature megakaryocytes for integrin studies and other biological applications, and they implicate CalDAG-GEFI in inside-out signaling to alphaIIbbeta3...
  74. pmc Essential role of Gas6 for glomerular injury in nephrotoxic nephritis
    Motoko Yanagita
    Department of Geriatric Medicine, Kyoto University, Kyoto, Japan
    J Clin Invest 110:239-46. 2002
    ..These data indicate that Gas6 induces glomerular cell proliferation in NTN and suggest that this factor contributes to glomerular injury and the progression of chronic nephritis...
  75. ncbi request reprint Gas6 induces Akt/mTOR-mediated mesangial hypertrophy in diabetic nephropathy
    Kojiro Nagai
    Department of Geriatric Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Kidney Int 68:552-61. 2005
    ..We have already reported Gas6 is involved in glomerular hypertrophy observed in diabetic nephropathy. However, the molecular mechanisms involved in glomerular hypertrophy are still unknown, especially in vivo...
  76. ncbi request reprint Growth arrest-specific gene 6 is involved in glomerular hypertrophy in the early stage of diabetic nephropathy
    Kojiro Nagai
    Department of Geriatric Medicine, Kyoto University Graduate School of Medicine, Kyoto 606 8507, Japan
    J Biol Chem 278:18229-34. 2003
    ..Inhibition of the Gas6/Axl pathway in diabetic patients might be beneficial to slow down the progression of diabetic nephropathy...
  77. ncbi request reprint Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and multiple deformations during mgcl-1 deficient spermatogenesis leading to reduced fertility
    Mamiko Maekawa
    Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University, Chiba, Japan
    Arch Histol Cytol 67:335-47. 2004
    ..These observations provide important insights into the mechanisms regulating the nuclear architecture and causes of human infertility...
  78. ncbi request reprint Development and analysis of megakaryocytes from murine embryonic stem cells
    Koji Eto
    Division of Vascular Biology, Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, VB 5, La Jolla, California 92037, USA
    Methods Enzymol 365:142-58. 2003
  79. ncbi request reprint Hyperplasia and carcinomas in Pten-deficient mice and reduced PTEN protein in human bladder cancer patients
    Hiroshi Tsuruta
    Department of Urology, Akita University School of Medicine, Akita, Japan
    Cancer Res 66:8389-96. 2006
    ..Thus, PTEN deficiency may contribute to bladder cancer both by initiating superficial papillary TCC and by promoting the progression of CIS to advanced invasive and metastatic forms...
  80. pmc The Grb2/Mek pathway represses Nanog in murine embryonic stem cells
    Takashi Hamazaki
    Department of Pathology, University of Florida College of Medicine, P O Box 100275, Gainesville, FL 32610, USA
    Mol Cell Biol 26:7539-49. 2006
    ..These data indicate that the Grb2/Mek pathway primarily mediates Nanog gene repression upon ES cell differentiation into primitive endoderm...
  81. ncbi request reprint Characterization of the piRNA complex from rat testes
    Nelson C Lau
    Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    Science 313:363-7. 2006
    ..Piwi has been genetically linked to TGS in flies, and slicer activity cofractionates with the purified complex. These results are consistent with a gene-silencing role for piRC in mammals...
  82. ncbi request reprint Stella is a maternal effect gene required for normal early development in mice
    Bernhard Payer
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Curr Biol 13:2110-7. 2003
    ..These findings suggest a similar role for STELLA during early human development as in mice and a potential involvement in germ cell tumors...
  83. pmc Cross talk between retinoic acid signaling and transcription factor GATA-2
    Shinobu Tsuzuki
    Section of Gene Function and Regulation, Institute of Cancer Research, London SW3 6JB, UK
    Mol Cell Biol 24:6824-36. 2004
    ....
  84. ncbi request reprint [Recent topics on leukemia: Leukemia and regenerative medicine]
    Toru Nakano
    Nihon Naika Gakkai Zasshi 92:1043-7. 2003
  85. ncbi request reprint A stromal cell-derived membrane protein that supports hematopoietic stem cells
    Hiroo Ueno
    Virology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Nat Immunol 4:457-63. 2003
    ..These results contribute to our understanding of the mechanisms by which the hematopoietic microenvironment regulates hematopoiesis...
  86. ncbi request reprint Identification of Gas6, a putative ligand for Sky and Axl receptor tyrosine kinases, as a novel neurotrophic factor for hippocampal neurons
    Hiroshi Funakoshi
    Division of Molecular Regenerative Medicine, Course of Advanced Medicine, Osaka University Graduate School of Medicine, B 7, Osaka 565 0871, Japan
    J Neurosci Res 68:150-60. 2002
    ..Gas6 showed regulated expression in the sciatic nerve after nerve transection. Therefore, Sky and Gas6 have neurotrophic roles in the nervous system...
  87. pmc Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice
    Shigehisa Yanagi
    Department of Molecular Biology, Akita University School of Medicine, Akita, Japan
    J Clin Invest 117:2929-40. 2007
    ..These results indicate that Pten is essential for both normal lung morphogenesis and the prevention of lung carcinogenesis, possibly because this tumor suppressor is required for BASC homeostasis...
  88. doi request reprint Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 453:539-43. 2008
    ..Our results reveal a role for endogenous siRNAs in mammalian oocytes and show that organisms lacking RdRP activity can produce functional endogenous siRNAs from naturally occurring dsRNAs...
  89. ncbi request reprint Testicular teratomas: back to pluripotent stem cells
    Tohru Kimura
    Department of Pathology, Osaka University Medical School, Japan
    Prog Mol Subcell Biol 40:133-50. 2005
  90. ncbi request reprint The Pten/PI3K pathway governs the homeostasis of Valpha14iNKT cells
    Hiroyuki Kishimoto
    Department of Molecular Biology, Akita University School of Medicine, Hondo 1 1 1, Akita 0108 543, Japan
    Blood 109:3316-24. 2007
    ..Thus, the Pten/PI3K pathway is indispensable for the homeostasis and antitumor surveillance function of Valpha14iNKT cells...
  91. ncbi request reprint Growth arrest-specific gene 6 and Axl signaling enhances gastric cancer cell survival via Akt pathway
    Tateo Sawabu
    Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Mol Carcinog 46:155-64. 2007
    ..Our results suggests that Gas6-Axl signaling plays a role during gastric carcinogenesis, and that targeting Gas6-Axl signaling could be a novel therapeutic for gastric cancer...
  92. ncbi request reprint Isolation and characterization of LNCaP sublines differing in hormone sensitivity
    Kazuhiro Iguchi
    Laboratory of Pharmaceutics, Gifu Pharmaceutical University, 5 6 1 Mitahora Higashi, Gifu 502 8585, Japan
    J Androl 28:670-8. 2007
    ..5 times higher than that in G4 tumors. LNCaP-E9 cells show lower androgen sensitivity than LNCaP-G4 cells. E9 and G4 cells would be helpful for understanding the biology of hormone-refractory prostate cancer...
  93. ncbi request reprint Akt mediates self-renewal division of mouse spermatogonial stem cells
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 134:1853-9. 2007
    ..These results demonstrate that activation of the PI3K-Akt pathway plays a central role in the self-renewal division of spermatogonial stem cells...
  94. ncbi request reprint Effect of Gas6 on secretory phospholipase A(2)-IIA-induced apoptosis in cortical neurons
    Tatsurou Yagami
    Discovery Research Laboratories, Shionogi and Co Ltd, 12 4 Sagisu 5 chome, Fukushima ku, Osaka 553 0002, Japan
    Brain Res 985:142-9. 2003
    ..In conclusion, we demonstrate that Gas6 rescues cortical neurons from sPLA(2)-IIA-induced apoptosis. Furthermore, the present study indicates that inhibition of L-VSCC contributes to the neuroprotective effect of Gas6...
  95. ncbi request reprint Physiological functions of Pten in mouse tissues
    Hiroyuki Kishimoto
    Department of Biochemistry, Akita University School of Medicine, Hondo 1 1 1, Akita 010 8543, Japan
    Cell Struct Funct 28:11-21. 2003
    ..This review will summarize the phenotypes of conditional mutant mice lacking Pten function in specific tissues, and discuss how these phenotypes relate to the physiological roles of Pten in various organ systems...
  96. doi request reprint Portrait of PTEN: messages from mutant mice
    Akira Suzuki
    Division of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    Cancer Sci 99:209-13. 2008
    ..The present review will summarize results obtained from the study of conditional mutant mice lacking Pten in specific tissues, and discuss the possible biological and molecular explanations for why Pten deficiency leads to tumorigenesis...
  97. ncbi request reprint Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation
    Kazuta Yasui
    Osaka Red Cross Blood Center, Japan
    Stem Cells 21:143-51. 2003
    ..We conclude that the delayed recovery of platelets after CB transplantation is due to both subpopulation distribution and the process of differentiation from AC133(+)CD34(+) cells...
  98. doi request reprint Effective clearance of intracellular Leishmania major in vivo requires Pten in macrophages
    Shoko Kuroda
    Department of Molecular Biology, Akita University Graduate School of Medicine, Akita, Japan
    Eur J Immunol 38:1331-40. 2008
    ..major infection, in line with the ability of LysMCrePten(flox/flox) mice to eventually eliminate the parasite. Our results indicate that the efficient clearance of intracellular parasites requires Pten in macrophages...
  99. ncbi request reprint A novel class of small RNAs bind to MILI protein in mouse testes
    Alexei Aravin
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 442:203-7. 2006
    ..The identification of this new class of small RNAs provides an important starting point to determine the molecular function of Piwi proteins in mammalian spermatogenesis...