S Nagata

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi Apoptotic DNA fragmentation
    S Nagata
    Department of Genetics, Osaka University Medical School and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Exp Cell Res 256:12-8. 2000
  2. pmc Toll-like receptor-independent gene induction program activated by mammalian DNA escaped from apoptotic DNA degradation
    Yasutaka Okabe
    Department of Genetics, Osaka University Medical School, Japan
    J Exp Med 202:1333-9. 2005
  3. ncbi Human autoimmune lymphoproliferative syndrome, a defect in the apoptosis-inducing Fas receptor: a lesson from the mouse model
    S Nagata
    Department of Genetics, Osaka University Medical School, Japan
    J Hum Genet 43:2-8. 1998
  4. ncbi Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens
    Sogo Nishimoto
    Department of Genetics, Osaka University Medical School, Osaka 565 0871, Japan
    Nature 424:1071-4. 2003
  5. ncbi Apoptosis and autoimmune diseases
    Shigekazu Nagata
    Laboratory of Genetics, Department of Integrated Biology, Graduate School of Frontier Bioscience, Osaka University, Japan
    IUBMB Life 58:358-62. 2006
  6. ncbi Breakdown of chromosomal DNA
    Shigekazu Nagata
    Department of Genetics, Osaka University Medical School, Suita, Japan
    Cornea 21:S2-6. 2002
  7. ncbi Degradation of chromosomal DNA during apoptosis
    S Nagata
    Integrated Biology Laboratories, Graduate School of Frontier Science, Japan Science and Technology Corperation, Suita, Osaka
    Cell Death Differ 10:108-16. 2003
  8. ncbi DNA degradation in development and programmed cell death
    Shigekazu Nagata
    Laboratory of Genetics, Integrated Biology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Japan
    Annu Rev Immunol 23:853-75. 2005
  9. ncbi Fas ligand-induced apoptosis
    S Nagata
    Department of Genetics, Osaka Medical School, Japan
    Annu Rev Genet 33:29-55. 1999
  10. ncbi Identification and developmental expression of inhibitor of caspase-activated DNase (ICAD) in Drosophila melanogaster
    N Mukae
    Department of Genetics, Osaka University Medical School and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Osaka 565 0871, Japan
    J Biol Chem 275:21402-8. 2000

Collaborators

Detail Information

Publications77

  1. ncbi Apoptotic DNA fragmentation
    S Nagata
    Department of Genetics, Osaka University Medical School and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Exp Cell Res 256:12-8. 2000
    ..In this review, the molecular mechanism of and the physiological roles played by apoptotic DNA fragmentation will be discussed...
  2. pmc Toll-like receptor-independent gene induction program activated by mammalian DNA escaped from apoptotic DNA degradation
    Yasutaka Okabe
    Department of Genetics, Osaka University Medical School, Japan
    J Exp Med 202:1333-9. 2005
    ..These results indicate that there is a TLR-independent sensing mechanism to activate the innate immunity for the endogenous DNA escaping lysosomal degradation...
  3. ncbi Human autoimmune lymphoproliferative syndrome, a defect in the apoptosis-inducing Fas receptor: a lesson from the mouse model
    S Nagata
    Department of Genetics, Osaka University Medical School, Japan
    J Hum Genet 43:2-8. 1998
    ..This is a good example of a case in which the identification of a mouse mutation has led to the understanding of a human disease...
  4. ncbi Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens
    Sogo Nishimoto
    Department of Genetics, Osaka University Medical School, Osaka 565 0871, Japan
    Nature 424:1071-4. 2003
    ..These results indicate that DLAD is responsible for the degradation of nuclear DNA during lens cell differentiation, and that if DNA is left undigested in the lens, it causes cataracts of the nucleus lentis, blocking the light path...
  5. ncbi Apoptosis and autoimmune diseases
    Shigekazu Nagata
    Laboratory of Genetics, Department of Integrated Biology, Graduate School of Frontier Bioscience, Osaka University, Japan
    IUBMB Life 58:358-62. 2006
    ..The apoptotic process and the diseases caused by defects in it are briefly reviewed...
  6. ncbi Breakdown of chromosomal DNA
    Shigekazu Nagata
    Department of Genetics, Osaka University Medical School, Suita, Japan
    Cornea 21:S2-6. 2002
    ..In this article, the molecular mechanism of the apoptotic DNA degradation and the enucleation process of erythroid cells are discussed...
  7. ncbi Degradation of chromosomal DNA during apoptosis
    S Nagata
    Integrated Biology Laboratories, Graduate School of Frontier Science, Japan Science and Technology Corperation, Suita, Osaka
    Cell Death Differ 10:108-16. 2003
    ..Several other endonucleases have also been suggested as candidate effectors for the apoptotic degradation of chromosomal DNA. In this review, we will discuss the mechanism and role of DNA degradation during apoptosis...
  8. ncbi DNA degradation in development and programmed cell death
    Shigekazu Nagata
    Laboratory of Genetics, Integrated Biology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Japan
    Annu Rev Immunol 23:853-75. 2005
    ..I discuss what might be or will be learned from understanding the molecular mechanisms of DNA degradation that occurs during mammalian development...
  9. ncbi Fas ligand-induced apoptosis
    S Nagata
    Department of Genetics, Osaka Medical School, Japan
    Annu Rev Genet 33:29-55. 1999
    ..Loss-of-function mutations in the signaling molecules involved in apoptosis cause hyper-proliferation of cells in mouse and human. In contrast, exaggeration of this death cascade causes the destruction of various tissues...
  10. ncbi Identification and developmental expression of inhibitor of caspase-activated DNase (ICAD) in Drosophila melanogaster
    N Mukae
    Department of Genetics, Osaka University Medical School and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Osaka 565 0871, Japan
    J Biol Chem 275:21402-8. 2000
    ..Northern hybridization and the immunohistochemical analyses revealed that the expression of the dICAD gene is developmentally regulated...
  11. ncbi A novel activation mechanism of caspase-activated DNase from Drosophila melanogaster
    H Yokoyama
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 275:12978-86. 2000
    ..These results indicated that unlike mammalian CAD, Drosophila CAD must be cleaved by caspases to be activated...
  12. ncbi Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver
    K Kawane
    Department of Genetics, Osaka University Medical School, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Suita, Osaka 565 0871, Japan
    Science 292:1546-9. 2001
    ..Thus, DNase II in macrophages appears to be responsible for destroying the nuclear DNA expelled from erythroid precursor cells...
  13. ncbi Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis
    H Sakahira
    Department of Genetics, Osaka University Medical School, Suita, Japan
    Nature 391:96-9. 1998
    ..Our results indicate that activation of CAD downstream of the caspase cascade is responsible for internucleosomal DNA degradation during apoptosis, and that ICAD works as an inhibitor of this process...
  14. ncbi The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells
    T Fukumoto
    Department of Genetics, B 3, Osaka University Medical School, and Core Research for Evolutional Science and Technology, Japan
    J Biol Chem 276:38441-8. 2001
    ....
  15. ncbi A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
    M Enari
    Department of Genetics, Osaka University Medical School, Suita, Japan
    Nature 391:43-50. 1998
    ....
  16. pmc Signals transducers and activators of transcription (STAT)-induced STAT inhibitor-1 (SSI-1)/suppressor of cytokine signaling-1 (SOCS-1) suppresses tumor necrosis factor alpha-induced cell death in fibroblasts
    Y Morita
    Department of Medicine III, Osaka University Medical School, 2 2, Yamada oka, Suita City, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:5405-10. 2000
    ..These findings suggest that SSI-1 suppresses TNF-alpha-induced cell death, which is mediated by p38 MAP kinase signaling...
  17. ncbi Enzymatic active site of caspase-activated DNase (CAD) and its inhibition by inhibitor of CAD
    H Sakahira
    Department of Genetics, Osaka University Medical School, Japan Science and Technology Corporation, Suita
    Arch Biochem Biophys 388:91-9. 2001
    ..This molecular mechanism for the inhibition of CAD DNase by ICAD is similar to that proposed for colicin endonuclease and its inhibitor, immunity protein...
  18. ncbi Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC1) receptor
    H Hashimoto
    Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565 0871, Japan
    Genomics 58:90-3. 1999
    ..The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped...
  19. ncbi Structure of the heterodimeric complex between CAD domains of CAD and ICAD
    T Otomo
    Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Struct Biol 7:658-62. 2000
    ..This implies that the interaction between the CAD domains of CAD and ICAD is an essential step in the correct folding of CAD in the complex...
  20. ncbi Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptide
    H Hashimoto
    Laboratories of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
    J Neurochem 75:1810-7. 2000
    ..We suggest that the mutant mice with markedly reduced PAC(1)R expression can serve as a useful animal model or cell culture system for further studies in PAC(1)R function...
  21. ncbi Caspase 1-independent IL-1beta release and inflammation induced by the apoptosis inducer Fas ligand
    K Miwa
    Osaka Bioscience Institute, Department of Molecular Biology, Suita, Japan
    Nat Med 4:1287-92. 1998
    ..These results demonstrate a newly discovered role for Fas ligand in inflammation, and challenge the dogma that apoptosis does not induce inflammation...
  22. pmc Molecular cloning and characterization of human caspase-activated DNase
    N Mukae
    Department of Genetics, Osaka University Medical School, B 3, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:9123-8. 1998
    ..Overexpression of CAD potentiated DNA fragmentation by apoptotic stimuli in these cell lines, indicating that CAD is responsible for the apoptotic DNA degradation...
  23. ncbi Processing of tumor necrosis factor by the membrane-bound TNF-alpha-converting enzyme, but not its truncated soluble form
    T Itai
    Department of Genetics, Osaka University Medical School, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Osaka, Japan
    Eur J Biochem 268:2074-82. 2001
    ..These results suggest that binding of the membrane-anchored but not the soluble form of TACE to TNF-alpha results in efficient ectodomain shedding, and that FasL secretase is a metalloproteinase similar, but not identical, to TACE...
  24. pmc Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing
    T Suda
    Osaka Bioscience Institute, Department of Molecular Biology, Suita, Osaka 565, Japan
    J Exp Med 186:2045-50. 1997
    ..Soluble FasL inhibited the killing of fresh PBT by membrane FasL. These results indicate that the shedding of FasL from the membrane is a mechanism for downregulating at least part of its killing activity...
  25. pmc Myeloid-specific transcriptional activation by murine myeloid zinc-finger protein 2
    K Murai
    Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Osaka 565, Japan
    Proc Natl Acad Sci U S A 95:3461-6. 1998
    ..The middle region recruits a coactivator, which is responsible for myeloid-specific transcriptional activation. The C-terminal zinc-finger domain functions as a DNA-binding domain...
  26. ncbi Downregulation of Fas ligand by shedding
    M Tanaka
    Department of Genetics, Osaka University Medical School, Suita, Japan
    Nat Med 4:31-6. 1998
    ..These results indicate that the membrane-bound form of FasL is the functional form and suggest that shedding of FasL is to prevent the killing of the healthy bystander cells by cytotoxic T cells...
  27. ncbi Frequent mutations of Fas gene in thyroid lymphoma
    T Takakuwa
    Department of Pathology, Osaka University Graduate School of Medicine, Suita, Japan
    Cancer Res 61:1382-5. 2001
    ..These findings suggested that accumulation of lymphoid cells in CLTH with Fas mutation provides a basis for development of TL...
  28. ncbi A novel form of the myeloid-specific zinc finger protein (MZF-2)
    K Murai
    Osaka Bioscience Institute, Suita, Japan
    Genes Cells 2:581-91. 1997
    ..Human myeloid zinc finger protein (MZF-1) is a putative transcription factor containing 13 zinc fingers, and has been suggested that it regulates the development of neutrophilic granulocytes...
  29. pmc Activation of the innate immunity in Drosophila by endogenous chromosomal DNA that escaped apoptotic degradation
    Naomi Mukae
    Department of Genetics, Osaka University Medical School, Osaka 565 0871, Japan
    Genes Dev 16:2662-71. 2002
    ..These results indicated that CAD and DNase II work independently to degrade chromosomal DNA during apoptosis, and if the DNA is left undigested, it can activate the innate immunity in Drosophila...
  30. ncbi SOCS-1 suppresses TNF-alpha-induced apoptosis through the regulation of Jak activation
    Akihiro Kimura
    Department of Molecular Medicine, Osaka University Graduate School of Medicine, 2 2, Yamada oka, Suita, Osaka, 565 0871, Japan
    Int Immunol 16:991-9. 2004
    ..Taken together, these results suggest that SOCS-1 inhibits TNF-alpha-induced apoptosis through regulation of Jaks...
  31. ncbi Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation
    Kohki Kawane
    Department of Genetics, Osaka University Medical School, Japan
    Nat Immunol 4:138-44. 2003
    ....
  32. ncbi Natural killer cell-derived large granular lymphocyte lymphoma of lung developed in a patient with hypersensitivity to mosquito bites and reactivated Epstein-Barr virus infection
    M Mizuki
    Department of Hematology and Oncology, Osaka University Medical School, Suita, Japan
    Am J Hematol 59:309-15. 1998
    ..This hypercytokinemia, reflecting the microenvironment of lymphoma cells, may play a role in the progression of the lymphoma and organ injury in the lung...
  33. ncbi Fas gene mutations in prostatic intraepithelial neoplasia and concurrent carcinoma: analysis of laser capture microdissected specimens
    H Takayama
    Department of Pathology, Osaka University Medical School, Japan
    Lab Invest 81:283-8. 2001
    ..Occasional findings of LOH in HGPIN and PCA suggested that genetic instability might occur during the early phase of prostatic carcinogenesis...
  34. ncbi Degradation of nuclear DNA by DNase II-like acid DNase in cortical fiber cells of mouse eye lens
    Masaki Nakahara
    Department of Genetics, Osaka University Medical School, Japan
    FEBS J 274:3055-64. 2007
    ..These results suggest that the lysosomal system plays a role in the degradation of cellular organelles during lens cell differentiation...
  35. ncbi Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages
    Kohki Kawane
    Department of Genetics, Osaka University, Osaka 565 0871, Japan
    Nature 443:998-1002. 2006
    ....
  36. ncbi MFG-E8-dependent clearance of apoptotic cells, and autoimmunity caused by its failure
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, Osaka, Japan
    Curr Dir Autoimmun 9:162-72. 2006
    ..In this review, we discuss the importance of the MFG-E8-mediated clearance of apoptotic cells in the prevention of autoimmune diseases...
  37. pmc Activation of distinct caspase-like proteases by Fas and reaper in Drosophila cells
    T Kondo
    Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Osaka 565, Japan
    Proc Natl Acad Sci U S A 94:11951-6. 1997
    ....
  38. ncbi Long-lasting antagonistic effects of monatepil on Ca2+ current in guinea-pig ventricular cells
    S Nagata
    Department of Pharmacology I, Dainippon Pharmaceutical Co, Ltd, Osaka, Japan
    Arzneimittelforschung 45:1061-3. 1995
    ..2 Hz. IC50 value of monatepil for the peak ICa at +10 mV was 18.7 nmol/l. The ICa inhibition was still sustained 30 min after washout, indicating that monatepil had long lasting activity in the mammalian single muscle cell...
  39. ncbi Apoptosis by death factor
    S Nagata
    Department of Genetics, Osaka University Medical School, Japan
    Cell 88:355-65. 1997
  40. ncbi Swapping between Fas and granulocyte colony-stimulating factor receptor
    T Takahashi
    Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Japan
    J Biol Chem 271:17555-60. 1996
    ....
  41. ncbi Different modes of sensory neuropeptides and nitric oxide involvement in relaxation of guinea-pig vessels
    H Matsuda
    Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Osaka, Japan
    J Auton Nerv Syst 55:115-22. 1995
    ..Thus, CGRP could be significantly responsible for the NO-independent NANC relaxation in AMA, whereas both CGRP and SP could additionally relax PCV in a NO-dependent manner...
  42. pmc Requirement of Fas for the development of autoimmune diabetes in nonobese diabetic mice
    N Itoh
    Department of Clinical Laboratory Science, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Osaka 565, Japan
    J Exp Med 186:613-8. 1997
    ..These results suggest that Fas-mediated cytotoxicity is required to initiate beta cell autoimmunity in NOD mice. Fas-Fas ligand system might be critical for autoimmune beta cell destruction leading to IDDM...
  43. ncbi Binding of NF-Y transcription factor to one of the cis-elements in the myeloperoxidase gene promoter that responds to granulocyte colony-stimulating factor
    T Orita
    Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Osaka 565, Japan
    J Biol Chem 272:23216-23. 1997
    ..The recombinant NF-Y was then shown to bind to GRE in a combination of the three subunits...
  44. ncbi Identification of CCR2, flotillin, and gp49B genes as new G-CSF targets during neutrophilic differentiation
    Satoshi Iida
    Department of Genetics, B 3, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Leukoc Biol 78:481-90. 2005
    ..We show that bone marrow neutrophils express functional CCR2, which suggest that CC chemokines may play previously unknown roles in neutrophil activation and chemotaxis...
  45. ncbi Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Science 304:1147-50. 2004
    ..These data demonstrate that MFG-E8 has a critical role in removing apoptotic B cells in the germinal centers and that its failure can lead to autoimmune diseases...
  46. ncbi Lethal anemia caused by interferon-beta produced in mouse embryos carrying undigested DNA
    Hideyuki Yoshida
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Nat Immunol 6:49-56. 2005
    ....
  47. ncbi The active molecular form of plasma adrenomedullin is extracted in the pulmonary circulation in patients with mitral stenosis: possible role of adrenomedullin in pulmonary hypertension
    T Nishikimi
    Department of Medicine, National Cardiovascular Center, 5 7 1, Fujishirodai, Suita, Osaka 565 8565, Japan
    Clin Sci (Lond) 100:61-6. 2001
    ....
  48. ncbi Clinical analysis of myocardial perfusion and metabolism in patients with hypertrophic cardiomyopathy by single photon emission tomography and positron emission tomography
    Y Ishida
    Department of Radiology and Nuclear Medicine, National Cardiovascular Center, Fujishirodai 5-7-1, Suita, Osaka 565-8565, Japan
    J Cardiol 37:121-8. 2001
    ..The significance of 18F-FDG in patients with hypertrophic cardiomyopathy is still uncertain...
  49. ncbi Novel missense mutation in cardiac troponin T gene found in Japanese patient with hypertrophic cardiomyopathy
    C Nakajima-Taniguchi
    Department of Medicine III, Osaka University Medical School, Japan
    J Mol Cell Cardiol 29:839-43. 1997
    ..The disease in the kindred was severe and there was a high incidence of sudden or disease-related deaths in the kindred with this mutation...
  50. ncbi Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells
    Hideyuki Yoshida
    Department of Genetics, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Nature 437:754-8. 2005
    ..Fetal liver macrophages efficiently engulfed the nuclei; masking the phosphatidylserine on the nuclei with the dominant-negative form of milk-fat-globule EGF8 (MFG-E8) prevented this engulfment...
  51. pmc Masking of phosphatidylserine inhibits apoptotic cell engulfment and induces autoantibody production in mice
    Kenichi Asano
    Department of Genetics, Osaka University Medical School, Japan
    J Exp Med 200:459-67. 2004
    ..These results indicated that the impairment of apoptotic cell phagocytosis led to autoantibody production...
  52. ncbi Opposite effects of rho family GTPases on engulfment of apoptotic cells by macrophages
    Michio Nakaya
    Department of Genetics, Osaka University Medical School, Osaka 565 0871, Japan
    J Biol Chem 281:8836-42. 2006
    ..The dominant-negative form of RhoG or Rac1 inhibited this process, which suggested RhoG and Rac1 are also involved in the integrin-mediated engulfment...
  53. ncbi Tethering of apoptotic cells to phagocytes through binding of CD47 to Src homology 2 domain-bearing protein tyrosine phosphatase substrate-1
    Kazutoshi Tada
    Department of Genetics, Osaka University Medical School, Osaka, Japan
    J Immunol 171:5718-26. 2003
    ..These results indicate that macrophages require CD47 and phosphatidylserine on apoptotic cells for engulfment, and suggest that the interaction between CD47 and SHPS-1 works as a tethering step in the phagocytosis...
  54. ncbi Expression of developmental endothelial locus-1 in a subset of macrophages for engulfment of apoptotic cells
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, Osaka, Japan
    J Immunol 172:3876-82. 2004
    ..These results suggest the existence of macrophage subsets that use MFG-E8 or Del-1 differently to engulf apoptotic cells...
  55. ncbi A SWI2/SNF2-type ATPase/helicase protein, mDomino, interacts with myeloid zinc finger protein 2A (MZF-2A) to regulate its transcriptional activity
    Hironori Ogawa
    Department of Genetics B 3, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Genes Cells 8:325-39. 2003
    ..To understand the mechanism of the MZF-2A-dependent transcriptional activation, we screened for molecules that interact with the transactivation domain (TAD) of MZF-2A...
  56. ncbi Structure of the gene encoding the mouse pituitary adenylate cyclase-activating polypeptide receptor
    H Aino
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan
    Gene 164:301-4. 1995
    ..The proximal promoter region is highly G+C rich and lacks an apparent TATA box, but contains a CCAAT box and two potential Sp1-binding sites...
  57. ncbi Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide
    T Ishihara
    Osaka Bioscience Institute, Suita, Japan
    Neuron 8:811-9. 1992
    ..In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus...
  58. ncbi Regulation of myeloid zinc finger protein 2A transactivation activity through phosphorylation by mitogen-activated protein kinases
    Hironori Ogawa
    Department of Genetics B 3, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 278:2921-7. 2003
    ..Taken together, these results indicate that MZF-2A is a novel target for the ERK and p38 MAP kinase signaling pathways, and its transactivation activity is negatively regulated by MAP kinase-mediated phosphorylation of the TAD...
  59. ncbi Critical role of the p400/mDomino chromatin-remodeling ATPase in embryonic hematopoiesis
    Takeshi Ueda
    Laboratory of Genetics B 3, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Japan Science and Technology Corporation, Osaka, Japan
    Genes Cells 12:581-92. 2007
    ..These results suggest that p400/mDomino plays a critical role in embryonic hematopoiesis by regulating the expression of developmentally essential genes such as those in the Hox gene cluster...
  60. ncbi Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase
    Hideki Sakahira
    Department of Genetics, Osaka University Medical School, and Core Research for Evolutional Science and Technology, Japan
    J Biol Chem 277:3364-70. 2002
    ..These results indicate that ICAD together with Hsc70-Hsp40 assists the folding of CAD during its synthesis, and that the CAD*ICAD heterodimer is formed co-translationally...
  61. pmc Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination
    Hiroko Koike
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    EMBO Rep 3:433-7. 2002
    ..Expression of Cre induced the recombination between homologous chromosomes and led to an elevation in the number of biallelic mutants. This system will facilitate phenotype-driven gene function study in the mammalian system...
  62. pmc Impaired involution of mammary glands in the absence of milk fat globule EGF factor 8
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 102:16886-91. 2005
    ..These results demonstrate that MFG-E8-mediated phagocytosis of apoptotic epithelial cells and MFGs is important for efficient involution of mammary glands...
  63. ncbi Expression of milk fat globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells
    Kay Miyasaka
    Laboratory of Genetics, Integrated Biology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    Eur J Immunol 34:1414-22. 2004
    ..These results indicated that MFG-E8 plays an essential role in the engulfment of apoptotic cells by bone marrow-derived immature DC...
  64. pmc Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury
    Masato Koike
    Department of Cell Biology and Neurosciences, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Am J Pathol 172:454-69. 2008
    ....
  65. ncbi Requirement of Fas expression in B cells for tolerance induction
    Hidehiro Fukuyama
    Department of Genetics, Osaka University Medical School, Osaka, Japan
    Eur J Immunol 32:223-30. 2002
    ..These results indicate that both mature B cells and T cells must undergo Fas-mediated apoptosis to prevent the development of autoimmune diseases...
  66. ncbi SEI family of nuclear factors regulates p53-dependent transcriptional activation
    Rie Watanabe-Fukunaga
    Department of Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Genes Cells 10:851-60. 2005
    ..These results indicate that the SEI family of nuclear proteins regulates p53 transcriptional activity and a p53-independent signaling pathway leading to growth inhibition...
  67. ncbi DNase II and the Chk2 DNA damage pathway form a genetic barrier blocking replication of horizontally transferred DNA
    Anna Bergsmedh
    Cancer Center Karolinska Hospital, Karolinska Institutet, S 171 76 Stockholm, Sweden
    Mol Cancer Res 4:187-95. 2006
    ....
  68. ncbi Rheumatoid polyarthritis caused by a defect in DNA degradation
    Shigekazu Nagata
    Department of Medical Science, Graduate School of Medicine, Kyoto University, Yoshida Konoe, 606 8501 Kyoto, Japan
    Cytokine Growth Factor Rev 19:295-302. 2008
    ..Here, I summarize recent findings on inflammatory diseases induced by such defects in DNA degradation...
  69. ncbi Autoimmune diseases caused by defects in clearing dead cells and nuclei expelled from erythroid precursors
    Shigekazu Nagata
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Immunol Rev 220:237-50. 2007
    ..In contrast, failure to efficiently degrade the DNA of dead cells or erythroid cell nuclei activates innate immunity, causing lethal anemia in the fetus and chronic arthritis in adults...
  70. pmc Spatiotemporal activation of Rac1 for engulfment of apoptotic cells
    Michio Nakaya
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 105:9198-203. 2008
    ..These results indicate that activated Rac1 is necessary to assemble F-actin, but closing the phagocytic cup requires Rac1 to be deactivated...
  71. doi Imaging of Rab5 activity identifies essential regulators for phagosome maturation
    Masahiro Kitano
    Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Nature 453:241-5. 2008
    ..We therefore propose a mechanistic model in which the recruitment of Gapex-5 to phagosomes through the microtubule network induces the transient Rab5 activation...
  72. doi Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus
    Hiroshi Yamaguchi
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo, Kyoto 606 8501, Japan
    J Leukoc Biol 83:1300-7. 2008
    ..These results suggested that the aberrant expression of MFG-E8 is involved in the pathoetiology of some cases of hSLE...
  73. ncbi Identification of Tim4 as a phosphatidylserine receptor
    Masanori Miyanishi
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo ku, Kyoto 606 8501, Japan
    Nature 450:435-9. 2007
    ..These results indicate that Tim4 and Tim1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved...
  74. pmc Intraperitoneal injection of lipopolysaccharide induces dynamic migration of Gr-1high polymorphonuclear neutrophils in the murine abdominal cavity
    Shuichi Miyazaki
    Department of Microbiology, Toho University School of Medicine, Omori nishi 5 21 16, Ota ku, Tokyo 143 8540, Japan
    Clin Diagn Lab Immunol 11:452-7. 2004
    ..Our results suggest that LPS induced PMN death and that many PMNs expressing Gr-1(high) undergo apoptosis 180 min postinjection of LPS...
  75. ncbi Increased cytotoxicity of soluble Fas ligand by fusing isoleucine zipper motif
    Tetsuya Shiraishi
    Department of Neurosurgery, Faculty of Medicine, Saga University, Japan
    Biochem Biophys Res Commun 322:197-202. 2004
    ..The upper limit of FIZ-shFasL for safe systemic administration to rat is estimated as below 2 microg/ml in plasma concentration. FIZ-shFasL could be applicable as a therapeutic agent for cancer...
  76. ncbi MFG-E8 in the retina and retinal pigment epithelium of rat and mouse
    Barry L Burgess
    Jules Stein Eye Institute, UCLA Medical Center, Los Angeles, CA 90095 7000, USA
    Mol Vis 12:1437-47. 2006
    ....