Ryuichi Morishita

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint Therapeutic angiogenesis using hepatocyte growth factor (HGF)
    Ryuichi Morishita
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Curr Gene Ther 4:199-206. 2004
  2. doi request reprint Angiotensin receptor blocker prevented beta-amyloid-induced cognitive impairment associated with recovery of neurovascular coupling
    Shuko Takeda
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Hypertension 54:1345-52. 2009
  3. doi request reprint FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1
    Hiroki Hayashi
    Division of Gene Therapy Science, Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 29:909-14. 2009
  4. doi request reprint Hepatocyte growth factor, but not vascular endothelial growth factor, attenuates angiotensin II-induced endothelial progenitor cell senescence
    Fumihiro Sanada
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    Hypertension 53:77-82. 2009
  5. ncbi request reprint Angiogenesis induced by endothelial nitric oxide synthase gene through vascular endothelial growth factor expression in a rat hindlimb ischemia model
    Tsunetatsu Namba
    Division of Clinical Gene Therapy, Osaka University Medical School, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    Circulation 108:2250-7. 2003
  6. pmc Reduction of brain beta-amyloid (Abeta) by fluvastatin, a hydroxymethylglutaryl-CoA reductase inhibitor, through increase in degradation of amyloid precursor protein C-terminal fragments (APP-CTFs) and Abeta clearance
    Mitsuru Shinohara
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 285:22091-102. 2010
  7. ncbi request reprint In vivo evidence of angiogenesis induced by transcription factor Ets-1: Ets-1 is located upstream of angiogenesis cascade
    Naotaka Hashiya
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Circulation 109:3035-41. 2004
  8. ncbi request reprint No influence of tumor growth by intramuscular injection of hepatocyte growth factor plasmid DNA: safety evaluation of therapeutic angiogenesis gene therapy in mice
    Atsushi Matsuki
    Division of Gene Therapy Science, Biomedical Research Center, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 315:59-65. 2004
  9. ncbi request reprint Lack of association between the hepatocyte growth factor receptor, c-met, and the anti-apoptotic action of bag-1 in endothelial cells
    Hironori Nakagami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Japan
    Hypertens Res 27:359-65. 2004
  10. ncbi request reprint Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    Arterioscler Thromb Vasc Biol 25:2542-7. 2005

Detail Information

Publications131 found, 100 shown here

  1. ncbi request reprint Therapeutic angiogenesis using hepatocyte growth factor (HGF)
    Ryuichi Morishita
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Curr Gene Ther 4:199-206. 2004
    ..In this review, we will discuss a potential therapeutic strategy using HGF in cardiovascular disease...
  2. doi request reprint Angiotensin receptor blocker prevented beta-amyloid-induced cognitive impairment associated with recovery of neurovascular coupling
    Shuko Takeda
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Hypertension 54:1345-52. 2009
    ..These findings suggest the possibility that amelioration of cerebrovascular dysfunction with an angiotensin receptor blocker could be a novel therapeutic strategy for the early stage of Alzheimer disease...
  3. doi request reprint FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1
    Hiroki Hayashi
    Division of Gene Therapy Science, Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 29:909-14. 2009
    ..The goal of this study is to clear the mechanism of antiangiogenic signaling of FHL-2 in endothelial cells (ECs)...
  4. doi request reprint Hepatocyte growth factor, but not vascular endothelial growth factor, attenuates angiotensin II-induced endothelial progenitor cell senescence
    Fumihiro Sanada
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    Hypertension 53:77-82. 2009
    ..Potent induction of neovascularization of endothelial progenitor cells by HGF, but not VEGF, under angiotensin II was also confirmed by in vivo experiments using several models, including HGF transgenic mice...
  5. ncbi request reprint Angiogenesis induced by endothelial nitric oxide synthase gene through vascular endothelial growth factor expression in a rat hindlimb ischemia model
    Tsunetatsu Namba
    Division of Clinical Gene Therapy, Osaka University Medical School, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    Circulation 108:2250-7. 2003
    ....
  6. pmc Reduction of brain beta-amyloid (Abeta) by fluvastatin, a hydroxymethylglutaryl-CoA reductase inhibitor, through increase in degradation of amyloid precursor protein C-terminal fragments (APP-CTFs) and Abeta clearance
    Mitsuru Shinohara
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 285:22091-102. 2010
    ..These results have important implications for the development of disease-modifying therapy for Alzheimer disease as well as understanding of Abeta metabolism...
  7. ncbi request reprint In vivo evidence of angiogenesis induced by transcription factor Ets-1: Ets-1 is located upstream of angiogenesis cascade
    Naotaka Hashiya
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Circulation 109:3035-41. 2004
    ..Thus, we examined (1) the effects of overexpression of the ets-1 gene on angiogenesis in a rat hindlimb ischemia model, and (2) how ets-1 induced angiogenesis...
  8. ncbi request reprint No influence of tumor growth by intramuscular injection of hepatocyte growth factor plasmid DNA: safety evaluation of therapeutic angiogenesis gene therapy in mice
    Atsushi Matsuki
    Division of Gene Therapy Science, Biomedical Research Center, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 315:59-65. 2004
    ..Although recombinant HGF stimulated the growth of A431 cells in vitro, temporally and locally HGF elevation in hindlimb had no effect on tumor growth in mice...
  9. ncbi request reprint Lack of association between the hepatocyte growth factor receptor, c-met, and the anti-apoptotic action of bag-1 in endothelial cells
    Hironori Nakagami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Japan
    Hypertens Res 27:359-65. 2004
    ..Taken together, these results indicate that over-expression of bag-1 has an anti-apoptotic effect on endothelial cells independent of HGF signaling...
  10. ncbi request reprint Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    Arterioscler Thromb Vasc Biol 25:2542-7. 2005
    ..The delivery of autologous progenitor cells into ischemic tissue of patients is emerging as a novel therapeutic option. Here, we report the potential impact of cultured adipose tissue-derived cells (ADSC) on angiogenic cell therapy...
  11. pmc Diabetes-accelerated memory dysfunction via cerebrovascular inflammation and Abeta deposition in an Alzheimer mouse model with diabetes
    Shuko Takeda
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:7036-41. 2010
    ..Cerebrovascular changes and alteration in brain insulin signaling might play a pivotal role in this relationship. These findings could provide insights into this intensely debated association...
  12. ncbi request reprint Involvement of gamma-secretase in postnatal angiogenesis
    Hiroki Hayashi
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 363:584-90. 2007
    ....
  13. ncbi request reprint Local delivery of E2F decoy oligodeoxynucleotides using ultrasound with microbubble agent (Optison) inhibits intimal hyperplasia after balloon injury in rat carotid artery model
    Naotaka Hashiya
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Japan
    Biochem Biophys Res Commun 317:508-14. 2004
    ..A novel therapeutic strategy using E2F decoy ODN with Optison using ultrasound may be useful to inhibit restenosis in clinical practice without a viral vector...
  14. ncbi request reprint Hypertension accelerated experimental abdominal aortic aneurysm through upregulation of nuclear factor kappaB and Ets
    Suguru Shiraya
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertension 48:628-36. 2006
    ..Inhibition of NFkappaB and ets could be a novel therapeutic strategy to treat AAA in hypertensive patients...
  15. ncbi request reprint Novel therapeutic strategy to treat brain ischemia: overexpression of hepatocyte growth factor gene reduced ischemic injury without cerebral edema in rat model
    Munehisa Shimamura
    Professor, Division of Clinical Gene Therapy, Osaka University, Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Circulation 109:424-31. 2004
    ..Thus, in this study, we examined the therapeutic effects of HGF on brain injury in a rat permanent middle cerebral artery occlusion model...
  16. ncbi request reprint Inhibition of experimental abdominal aortic aneurysm in a rat model by the angiotensin receptor blocker valsartan
    Yoshikazu Fujiwara
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Int J Mol Med 22:703-8. 2008
    ..These data suggest that blockade of Ang II has an inhibitory effect on the development of AAA, independent of its antihypertensive effect...
  17. ncbi request reprint Transfection of human hepatocyte growth factor gene ameliorates secondary lymphedema via promotion of lymphangiogenesis
    Yukihiro Saito
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Circulation 114:1177-84. 2006
    ..Treatment for this disabling condition remains limited and largely ineffective. The goal of the present study was to investigate the therapeutic efficacy of hepatocyte growth factor (HGF) in animal models of lymphedema...
  18. pmc Decrease in blood pressure and regression of cardiovascular complications by angiotensin II vaccine in mice
    Futoshi Nakagami
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    PLoS ONE 8:e60493. 2013
    ..Taken together, these data indicate that vaccines targeting Ang II might be effective to decrease high BP and prevent cardiovascular complications without severe side effects...
  19. ncbi request reprint An efficient gene transfer method mediated by ultrasound and microbubbles into the kidney
    Hiromi Koike
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    J Gene Med 7:108-16. 2005
    ..From this point of view, it would be better to develop a novel non-viral efficient gene transfer method. Recently, it was reported that ultrasound exposure could induce cell membrane permeabilization and enhance gene expression...
  20. doi request reprint Development of a novel antimicrobial peptide, AG-30, with angiogenic properties
    Tomoyuki Nishikawa
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    J Cell Mol Med 13:535-46. 2009
    ..These data suggest that the novel peptide, AG-30, may have therapeutic potential for ischaemic diseases...
  21. ncbi request reprint Regression of abdominal aortic aneurysms by simultaneous inhibition of nuclear factor kappaB and ets in a rabbit model
    Takashi Miyake
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Circ Res 101:1175-84. 2007
    ..Minimally invasive molecular therapy targeted to the inhibition of NFkappaB and ets is expected to be useful for AAA through the rebalance of matrix synthesis and degradation...
  22. ncbi request reprint Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model
    Takashi Miyake
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Cardiovasc Res 79:706-14. 2008
    ..The purpose of this study was to determine the effects of simultaneous inhibition of these transcription factors on the formation of neointimal hyperplasia...
  23. pmc Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis
    Masaki Mori
    Division of Gene Therapy Science and Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 20:3115-24. 2009
    ..Thus, zyxin reorganizes actin fibers and enhances cell motility in response to TGF-beta1, thereby regulating EMT...
  24. ncbi request reprint Ubiquitin carboxyl-terminal hydrolase L1, a novel deubiquitinating enzyme in the vasculature, attenuates NF-kappaB activation
    Yoichi Takami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 27:2184-90. 2007
    ..Therefore, the goal of present study was to clarify the role of the UCHL1 gene in vascular remodeling by evaluating nuclear factor-kappaB (NF-kappaB) inactivation in ECs and vascular smooth muscle cells (VSMCs)...
  25. doi request reprint Hepatocyte growth factor inhibits lipopolysaccharide-induced oxidative stress via epithelial growth factor receptor degradation
    Kazutaka Shimizu
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 32:2687-93. 2012
    ..Here, we examined whether HGF can protect against systemic inflammatory response syndrome induced by LPS and investigated the mechanism...
  26. doi request reprint Role of central nervous system periostin in cerebral ischemia
    Munehisa Shimamura
    Department of Clinical Gene Therapy, Osaka University, Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Stroke 43:1108-14. 2012
    ..Here, we investigated the expression and role of periostin in the ischemic brain...
  27. ncbi request reprint HVJ-based non-viral gene transfer method: successful gene therapy using HGF and VEGF genes in experimental ischemia
    Munehisa Shimamura
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Japan
    Front Biosci 11:753-9. 2006
    ..This review discusses the feasibility of gene therapy using an HVJ-based non-viral vector containing the VEGF or HGF gene for cerebral ischemia...
  28. ncbi request reprint Increase in peripheral blood flow by intravenous administration of prostaglandin E1 in patients with peripheral arterial disease, accompanied by up-regulation of hepatocyte growth factor
    Hirofumi Makino
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Osaka, Japan
    Hypertens Res 27:85-91. 2004
    ..Also, our in vitro data suggested that PGE1 augmented not only the systemic HGF level, but also local HGF production, probably through cAMP accumulation, resulting in improvement of endothelial function and blood flow...
  29. ncbi request reprint Enhanced therapeutic angiogenesis by cotransfection of prostacyclin synthase gene or optimization of intramuscular injection of naked plasmid DNA
    Kazuya Hiraoka
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    Circulation 108:2689-96. 2003
    ..From this viewpoint, we performed the following modifications: (1) combination with vasodilation using prostacyclin and (2) changing the agents or volume of naked plasmid DNA in vivo...
  30. doi request reprint Estrogen attenuates vascular remodeling in Lp(a) transgenic mice
    Futoshi Nakagami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Japan
    Atherosclerosis 211:41-7. 2010
    ..Thus, it is mandatory to develop novel therapy to prevent atherosclerosis in high Lp(a) concentration. Here, we focused on the effect of estrogen on Lp(a) level and Lp(a)-induced vascular remodeling...
  31. ncbi request reprint Novel mechanisms of valsartan on the treatment of acute myocardial infarction through inhibition of the antiadhesion molecule periostin
    Kazuma Iekushi
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertension 49:1409-14. 2007
    ..The inhibition of periostin by valsartan might especially contribute to its beneficial effects on cardiac remodeling after myocardial infarction...
  32. doi request reprint Phase I/IIa clinical trial of therapeutic angiogenesis using hepatocyte growth factor gene transfer to treat critical limb ischemia
    Ryuichi Morishita
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 31:713-20. 2011
    ..To evaluate the safety and feasibility of intramuscular gene transfer using naked plasmid DNA-encoding hepatocyte growth factor (HGF) and to assess its potential therapeutic benefit in patients with critical limb ischemia...
  33. ncbi request reprint Delayed postischemic treatment with fluvastatin improved cognitive impairment after stroke in rats
    Munehisa Shimamura
    Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, The University of Tokyo, Japan
    Stroke 38:3251-8. 2007
    ..However, the effect of delayed postischemic treatment with statins is still unclear. In the present study, we evaluated the effects of fluvastatin in the chronic stage of cerebral infarction in a rat model...
  34. doi request reprint Telmisartan exerts renoprotective actions via peroxisome proliferator-activated receptor-γ/hepatocyte growth factor pathway independent of angiotensin II type 1 receptor blockade
    Hiroshi Kusunoki
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Hypertension 59:308-16. 2012
    ..Further development of the next generation of Ang II type 1 receptor blockers with added organ protective actions, such as PPARγ activation, might provide new beneficial drugs to treat renal and cardiovascular diseases...
  35. ncbi request reprint The effect of VEGF on blood vessels and blood cells during Xenopus development
    Nobutaka Koibuchi
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    Biochem Biophys Res Commun 344:339-45. 2006
    ....
  36. ncbi request reprint Gene therapy with an E2F transcription factor decoy inhibits cell cycle progression in rat anti-Thy 1 glomerulonephritis
    Naruya Tomita
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    Int J Mol Med 13:629-36. 2004
    ..These data also demonstrate the feasibility and the potential benefit of in vivo gene therapy as a novel strategy in the treatment of glomerular diseases...
  37. pmc Potential role of CYLD (Cylindromatosis) as a deubiquitinating enzyme in vascular cells
    Yoichi Takami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Osaka, Japan
    Am J Pathol 172:818-29. 2008
    ..In conclusion, these data demonstrate that the deubiquitinating enzyme CYLD may inhibit inflammation and proliferation in vascular cells and may represent a novel target for the treatment or prevention of atherosclerosis...
  38. doi request reprint Inhibition of development of experimental aortic abdominal aneurysm in rat model by atorvastatin through inhibition of macrophage migration
    Suguru Shiraya
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Atherosclerosis 202:34-40. 2009
    ..This study suggests new therapeutic aspects of statins to inhibit the progression of aneurysms...
  39. ncbi request reprint Angiogenic property of hepatocyte growth factor is dependent on upregulation of essential transcription factor for angiogenesis, ets-1
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Circulation 107:1411-7. 2003
    ..In this study, we addressed the following specific questions: (1) what genes responsible for angiogenesis can be regulated by HGF and (2) whether upregulation of gene expression for angiogenesis is dependent on ets-1...
  40. ncbi request reprint Gene transfer into adult rat spinal cord using naked plasmid DNA and ultrasound microbubbles
    Munehisa Shimamura
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    J Gene Med 7:1468-74. 2005
    ....
  41. doi request reprint Validation of Abeta1-40 administration into mouse cerebroventricles as an animal model for Alzheimer disease
    Shuko Takeda
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Brain Res 1280:137-47. 2009
    ..Symptomatic and pathophysiological similarities of this model to AD are quite important in considering the usefulness of this animal model. This validated animal model could be useful to develop and evaluate potential new drugs for AD...
  42. ncbi request reprint Development of novel method of non-viral efficient gene transfer into neonatal cardiac myocytes
    Hironori Tashiro
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Japan
    J Mol Cell Cardiol 39:503-9. 2005
    ....
  43. doi request reprint Do angiotensin receptor blockers protect against Alzheimer's disease?
    Hitomi Kurinami
    Division of Vascular Medicine and Epigenetics, Department of Child Development, United Graduate School of Child Development, Osaka University, Kanazawa University and Hamamatsu University School of Medicine, Suita, Japan
    Drugs Aging 30:367-72. 2013
    ....
  44. pmc Cardiac and renal protective effects of irbesartan via peroxisome proliferator-activated receptorγ-hepatocyte growth factor pathway independent of angiotensin II Type 1a receptor blockade in mouse model of salt-sensitive hypertension
    Hiroshi Kusunoki
    Department of Clinical Gene Therapy, Osaka University, Graduate School of Medicine, Suita, Japan
    J Am Heart Assoc 2:e000103. 2013
    ..In this study, we examined the organ-protective effects of irbesartan in a salt-sensitive hypertension model using AT1aR knockout mice...
  45. ncbi request reprint Inhibition of experimental abdominal aortic aneurysm progression by nifedipine
    Naruya Tomita
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    Int J Mol Med 21:239-44. 2008
    ....
  46. doi request reprint A model of cerebrovascular injury in rats
    Kouji Wakayama
    Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, University of Tokyo, Japan
    J Neurosci Methods 175:187-95. 2008
    ..These findings might shed new light on pathophysiology of post-angioplasty restenosis in intracranial arteries...
  47. ncbi request reprint Recent progress in gene therapy for cardiovascular disease
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Circ J 66:1077-86. 2002
    ..There is great potential in gene therapy for cardiovascular disease...
  48. ncbi request reprint Enhanced tumor-specific long-term immunity of hemagglutinating [correction of hemaggluttinating] virus of Japan-mediated dendritic cell-tumor fused cell vaccination by coadministration with CpG oligodeoxynucleotides
    Kazuya Hiraoka
    Division of Gene Therapy Science, Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan
    J Immunol 173:4297-307. 2004
    ..Thus, the combination of DC-tumor FCs and CpG ODN is an effective and feasible cancer vaccine to prevent the generation and recurrence of cancers...
  49. ncbi request reprint A calcium-channel blocker, benidipine, improves forearm reactive hyperemia in patients with essential hypertension
    Hirofumi Makino
    Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan
    Blood Press Suppl 1:39-44. 2005
    ..Interestingly, an increase in serum HGF concentration by benidipine might contribute to the improvement of endothelial dysfunction...
  50. ncbi request reprint Therapeutic option of plasmid-DNA based gene transfer
    Yoshiaki Taniyama
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    Curr Top Med Chem 12:1630-7. 2012
    ..This review focuses on plasmid DNA gene transfer and its enhancement, including ultrasound with microbubbles, electroporation, hydrodynamic method, gene gun, jet injection, cationic lipids and cationic polymers...
  51. ncbi request reprint Therapeutic effect of ribbon-type nuclear factor-κB decoy oligonucleotides in a rat model of inflammatory bowel disease
    Kazunari Ozaki
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Curr Gene Ther 12:484-92. 2012
    ....
  52. doi request reprint Long-term follow-up evaluation of results from clinical trial using hepatocyte growth factor gene to treat severe peripheral arterial disease
    Hirofumi Makino
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    Arterioscler Thromb Vasc Biol 32:2503-9. 2012
    ..We reported long-term evaluation of 2 years after HGF gene therapy in 22 patients with severe peripheral arterial disease...
  53. doi request reprint Estrogen inhibits vascular calcification via vascular RANKL system: common mechanism of osteoporosis and vascular calcification
    Mariana Kiomy Osako
    Division of Clinical Gene Therapy, Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Japan
    Circ Res 107:466-75. 2010
    ..The role of these proteins in vasculature, as well as the contribution of estrogen to vascular calcification, is poorly understood...
  54. doi request reprint Variation in OSBPL10 is associated with dyslipidemia
    Hiroshi Koriyama
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Yamada oka, Suita, Japan
    Hypertens Res 33:511-4. 2010
    ..003), with an odds ratio of 0.35 (95% confidence interval=0.17-0.76). Overall, the rs2290532 (D254N) polymorphism in OSBPL10 may predispose individuals with this SNP to hyper-LDL cholesterolemia...
  55. ncbi request reprint Safety evaluation of clinical gene therapy using hepatocyte growth factor to treat peripheral arterial disease
    Ryuichi Morishita
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Japan
    Hypertension 44:203-9. 2004
    ..Although the present data are conducted to demonstrate the safety as phase I/early phase IIa, the initial clinical outcome with HGF gene transfer seems to indicate usefulness as sole therapy for CLI...
  56. ncbi request reprint Targeted gene therapy for rat glomerulonephritis using HVJ-immunoliposomes
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 15 Yamada Oka, Suita 565 0871, Japan
    J Gene Med 4:527-35. 2002
    ..At experimental level several in vivo gene transfer methods have been reported...
  57. doi request reprint Elevation of plasma beta-amyloid level by glucose loading in Alzheimer mouse models
    Shuko Takeda
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Japan
    Biochem Biophys Res Commun 385:193-7. 2009
    ..These findings might provide a novel diagnostic tool for AD using the elevation of plasma Abeta level after glucose loading...
  58. doi request reprint Gene polymorphism of myospryn (cardiomyopathy-associated 5) is associated with left ventricular wall thickness in patients with hypertension
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertens Res 30:1239-46. 2007
    ..Our results suggest that the polymorphism of myospryn is associated with left ventricular hypertrophy, and an association between a Z-disc protein and cardiac adaptation in response to pressure overload...
  59. ncbi request reprint Model of vasculogenesis from embryonic stem cells for vascular research and regenerative medicine
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Japan
    Hypertension 48:112-9. 2006
    ..In the future, a human ES-based tissue model would be an optional tool for the screening of pharmaceutical drugs for vascular disease...
  60. ncbi request reprint Expression of hepatocyte growth factor and c-Met after spinal cord injury in rats
    Munehisa Shimamura
    Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, The University of Tokyo, Japan
    Brain Res 1151:188-94. 2007
    ..Since HGF plays a critical role in neurotrophic activity, activation of the HGF/c-Met signaling system might be involved in the process of post-traumatic regeneration...
  61. doi request reprint Cilnidipine, but not amlodipine, ameliorates osteoporosis in ovariectomized hypertensive rats through inhibition of the N-type calcium channel
    Hideo Shimizu
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertens Res 35:77-81. 2012
    ..These pleiotropic effects of antihypertensive drugs such as cilnidipine and carvedilol might provide additional benefits in the treatment of hypertensive postmenopausal women...
  62. ncbi request reprint Improvement of endothelial dysfunction by angiotensin II blockade accompanied by induction of vascular hepatocyte growth factor system in diabetic spontaneously hypertensive rats
    Keiko Matsumoto
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Heart Vessels 18:18-25. 2003
    ..The present data demonstrated the improvement of endothelial dysfunction by Ang II blockade in SHR-SM, accompanied by an increase in vascular HGF and c-met...
  63. ncbi request reprint Prevention of amyloid beta-induced memory impairment by fluvastatin, associated with the decrease in amyloid beta accumulation and oxidative stress in amyloid beta injection mouse model
    Hitomi Kurinami
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Int J Mol Med 21:531-7. 2008
    ..In clinical practice, the timing of the start of fluvastatin treatment might be critical in achieving a beneficial effect on cognitive function...
  64. ncbi request reprint Application of decoy oligodeoxynucleotides-based approach to renal diseases
    Naruya Tomita
    Division of Clinical Gene Therapy, Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Curr Drug Targets 5:717-33. 2004
    ..In this paper, we introduce the decoy strategy and demonstrate examples of application of decoy ODN approach targeting E2F and NF-kappaB in renal diseases...
  65. ncbi request reprint Essential role of HGF (hepatocyte growth factor) in blood formation in Xenopus
    Nobutaka Koibuchi
    Division of Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Blood 103:3320-5. 2004
    ..Overall, the present study demonstrated that HGF is necessary for primitive hematopoiesis by regulating the expression of SCL...
  66. ncbi request reprint Hypoxia-induced renal epithelial cell death through caspase-dependent pathway: role of Bcl-2, Bcl-xL and Bax in tubular injury
    Kei Yamamoto
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Int J Mol Med 14:633-40. 2004
    ..In addition, the present results demonstrate that translocation of Bax from the cytosol to the mitochondrial membrane occurred under hypoxia, thereby leading to pathological tissue destruction...
  67. ncbi request reprint Angiogenesis and antifibrotic action by hepatocyte growth factor in cardiomyopathy
    Yoshiaki Taniyama
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Hypertension 40:47-53. 2002
    ..These results suggest that HGF gene transfer may be useful to protect against myocardial injury in cardiomyopathy through its cardioprotective effects such as antifibrosis and angiogenesis actions...
  68. doi request reprint Hepatocyte growth factor attenuates transforming growth factor-β-angiotensin II crosstalk through inhibition of the PTEN/Akt pathway
    Kazuma Iekushi
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Hypertension 58:190-6. 2011
    ..Moreover, these renal protective effects were abrogated by a neutralizing antibody against HGF. Thus, the present study demonstrated that HGF counteracts the vicious cycle of Ang II-TGF-β1-AT(1)R, mediating the inhibition of PTEN...
  69. ncbi request reprint Hepatocyte growth factor prevents endothelial cell death through inhibition of bax translocation from cytosol to mitochondrial membrane
    Hironori Nakagami
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan
    Diabetes 51:2604-11. 2002
    ..HGF may be an important factor in the maintenance of endothelial function...
  70. ncbi request reprint Gene therapy with transcription factor decoy oligonucleotides as a potential treatment for cardiovascular diseases
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    Curr Drug Targets 4:339-46. 2003
    ....
  71. ncbi request reprint Development of high-throughput functional screening of therapeutic genes, using a hemagglutinating virus of Japan envelope vector
    Tomoyuki Nishikawa
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Hum Gene Ther 17:470-5. 2006
    ..Similarly, two growth-inhibiting genes were identified, the effects of which were similar to angiostatin. Overall, this novel system will help advance our understanding of cell biology and promote the utility of human gene therapy...
  72. doi request reprint HIF-1alpha signaling upstream of NKX2.5 is required for cardiac development in Xenopus
    Kaori Nagao
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    J Biol Chem 283:11841-9. 2008
    ..The cardiac abnormality in the HIF-1alpha-depleted embryo was restored with co-injection of nkx2.5 mRNA. Collectively, these findings reveal that HIF-1alpha-regulated nkx2.5 expression is required for heart development in Xenopus...
  73. ncbi request reprint Improvement of survival of skin flaps by combined gene transfer of hepatocyte growth factor and prostacyclin synthase
    Aya Nakagawa
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    J Gene Med 9:1087-94. 2007
    ....
  74. ncbi request reprint Cardiovascular disease and angiogenesis
    Ryuichi Morishita
    Department of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita
    Intern Med 42:301-2. 2003
  75. ncbi request reprint Local delivery of plasmid DNA into rat carotid artery using ultrasound
    Yoshiaki Taniyama
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Osaka, Japan
    Circulation 105:1233-9. 2002
    ..In this study, we examined the feasibility of a novel nonviral vector system by using high-frequency, low-intensity ultrasound irradiation for transfection into blood vessels...
  76. ncbi request reprint Hepatocyte growth factor as cardiovascular hormone: role of HGF in the pathogenesis of cardiovascular disease
    Ryuichi Morishita
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Endocr J 49:273-84. 2002
    ..In this review, we discussed the potential role of HGF in cardiovascular disease...
  77. ncbi request reprint Perspective in progress of cardiovascular gene therapy
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    J Pharmacol Sci 95:1-8. 2004
    ..This review focuses on the future potential of gene therapy for the treatment of cardiovascular disease...
  78. ncbi request reprint Therapeutic potential of decoy oligonucleotides strategy in cardiovascular diseases
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Expert Rev Cardiovasc Ther 1:463-70. 2003
    ....
  79. ncbi request reprint Potential therapeutic applications of decoy oligonucleotides
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Suita, Japan
    Curr Opin Mol Ther 4:166-70. 2002
    ....
  80. ncbi request reprint Obesity and gastrointestinal hormones-dual effect of angiotensin II receptor blockade and a partial agonist of PPAR-γ
    Hironori Nakagami
    Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita, Osaka, Japan
    Curr Vasc Pharmacol 9:162-6. 2011
    ..These favorable characteristics of telmisartan might be due to its action as a partial agonist of PPAR-γ, beyond its blood pressure-lowering effect, through Ang II blockade, which may be called "metabosartan"...
  81. ncbi request reprint New concept of vascular calcification and metabolism
    Hironori Nakagami
    Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Curr Vasc Pharmacol 9:124-7. 2011
    ..Based on these findings, we speculate that ezetimibe might prevent aortic intima calcification, which may give us the benefits to decrease vascular events...
  82. ncbi request reprint Transcription factors as molecular targets: molecular mechanisms of decoy ODN and their design
    Naruya Tomita
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Japan
    Curr Drug Targets 4:603-8. 2003
    ..In this section, we introduce the principles of the decoy strategy and how to design decoy ODN...
  83. ncbi request reprint Hemagglutinating virus of Japan (HVJ) envelope vector as a versatile gene delivery system
    Yasufumi Kaneda
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Osaka, 565 0871, Japan
    Mol Ther 6:219-26. 2002
    ..FITC-ODN were also delivered to spleen cells by intravenous injection of HVJ envelope. These results suggest that HVJ envelope vector will be useful for both ex vivo and in vivo gene therapy experiments...
  84. ncbi request reprint Transcription factor as molecular targets: is transcription factor decoy a novel drug?
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Osaka University, Graduate School of Medicine, Suita, Japan
    Curr Drug Targets 4:2 p before 599. 2003
  85. ncbi request reprint Oligonucleotide-based molecular therapy for restenosis after angioplasty
    Motokuni Aoki
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Suita, Japan
    Curr Drug Targets 4:613-8. 2003
    ..In this review, we have focused on the future potential of oligonucleotide-based gene therapy for restenosis after angioplasty, which still remains an issue in the field of cardiovascular disease...
  86. ncbi request reprint [Gene therapy in cardiovascular medicine as new pharmacological therapy]
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan 2 2 Yamada oka, Suita, Osaka 565, Japan
    Nihon Yakurigaku Zasshi 120:222-8. 2002
    ..In this review, we have focused on the future potential of gene therapy for the treatment of cardiovascular disease as a new pharmacological therapy...
  87. ncbi request reprint Does gene therapy become pharmacotherapy?
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    Exp Physiol 90:307-13. 2005
    ..This review focuses on the future potential of gene therapy for the treatment of cardiovascular disease. In the future, gene therapy might become a real pharmacotherapy to treat cardiovascular disease...
  88. ncbi request reprint Therapeutic potential of oligonucleotide-based therapy in cardiovascular disease
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Osaka, Japan
    BioDrugs 17:383-9. 2003
    ..In particular, early work relating to graft restenosis appears promising. The application of DNA-based therapies to regulate the transcription of disease-related genes in vivo has important therapeutic potential...
  89. ncbi request reprint A scientific rationale for the CREST trial results: evidence for the mechanism of action of cilostazol in restenosis
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Osaka 565, Japan
    Atheroscler Suppl 6:41-6. 2005
    ..These effects, in addition to antithrombotic and vasodilatory attributes of cilostazol, make it a potentially viable treatment option for preventing restenosis following coronary stenting...
  90. ncbi request reprint HMG-Co A reductase inhibitors in the treatment of cardiovascular diseases: stabilization of coronary artery plaque
    Ryuichi Morishita
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Suita, Japan
    Curr Drug Targets 3:379-85. 2002
    ..This article reviews the direct action of statins on the blood vessel wall, with reference to classification of statins based on difference in action on the blood vessel wall (hepatic statins vs. vascular statins)...
  91. ncbi request reprint Impairment of collateral formation in lipoprotein(a) transgenic mice: therapeutic angiogenesis induced by human hepatocyte growth factor gene
    Ryuichi Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan
    Circulation 105:1491-6. 2002
    ..In addition, we examined the feasibility of gene therapy by using an angiogenic growth factor, hepatocyte growth factor (HGF), to treat PAD in the presence of high Lp(a)...
  92. ncbi request reprint Molecular therapy to inhibit NFkappaB activation by transcription factor decoy oligonucleotides
    Ryuichi Morishita
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Curr Opin Pharmacol 4:139-46. 2004
    ..Transfection of cis-element double-stranded oligodeoxynucleotides has been reported as a powerful tool in a new class of anti-gene strategies for molecular therapy...
  93. ncbi request reprint Development of novel decoy oligonucleotides: advantages of circular dumb-bell decoy
    Naruya Tomita
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Suita City, Osaka 565 0871, Japan
    Curr Opin Mol Ther 5:107-12. 2003
    ..In this review, the applications of decoy ODN strategy and the advantages of modified CD double-stranded decoy ODNs will be discussed...
  94. ncbi request reprint Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    J Atheroscler Thromb 13:77-81. 2006
    ..These adipose tissue-derived cells have potential in angiogenic cell therapy for ischemic disease, and might be applied for regenerative cell therapy instead of bone marrow cells in the near future...
  95. ncbi request reprint Molecular therapy via transcriptional regulation with double-stranded oligodeoxynucleotides as decoys
    Hiroshige Nakamura
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University Medical School, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    In Vivo 16:45-8. 2002
    ..Diligent efforts to improve HVJ vectors as gene carriers and to expand the therapeutic applications of NFkB and E2F decoy ODNs will be important for the improved treatment of many genetic diseases...
  96. ncbi request reprint E2F decoy oligodeoxynucleotide ameliorates cartilage invasion by infiltrating synovium derived from rheumatoid arthritis
    Tetsuya Tomita
    Department of Orthopaedics, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Int J Mol Med 18:257-65. 2006
    ....
  97. ncbi request reprint [Development of plasmid DNA-based gene transfer]
    Yoshiaki Taniyama
    Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita City 565 0871, Japan
    Yakugaku Zasshi 126:1039-45. 2006
    ..Thus, we focused on the development of gene transfer using naked plasmid DNA with an ultrasound or microbubble-enhanced ultrasound method...
  98. ncbi request reprint Recent progress in cerebrovascular gene therapy
    Naoyuki Sato
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Curr Neurovasc Res 2:235-47. 2005
    ..Development of an effective and safe delivery system for a target gene will make human cerebrovascular gene therapy possible...
  99. ncbi request reprint [Molecular therapy for vascular disease using an oligonucleotide-based strategy]
    Takashi Miyake
    Nihon Yakurigaku Zasshi 129:158-62. 2007
  100. ncbi request reprint Intramuscular gene transfer of interleukin-10 cDNA reduces atherosclerosis in apolipoprotein E-knockout mice
    Masayuki Namiki
    Department of Internal Medicine, Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine, 7 5 2 Kusunoki cho, Chuo Ku, 650 0017, Kobe, Japan
    Atherosclerosis 172:21-9. 2004
    ..Therefore, IL-10 gene transfer changed the Th1 response and suppressed atherosclerotic lesion formation in apoE-KO mice. IL-10 could be a new target as a therapeutic tool for the treatment of atherosclerosis...
  101. ncbi request reprint Egr-1 in vascular smooth muscle cell proliferation in response to allo-antigen
    Yuko Wada
    The Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan
    J Surg Res 115:294-302. 2003
    ..This study examined our hypothesis that Egr-1 plays a critical role in the early stage of chronic cardiac allograft rejection and in the proliferation of the smooth muscle cell response to alloantigen...