R Morishita

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi request reprint HMG-Co A reductase inhibitors in the treatment of cardiovascular diseases: stabilization of coronary artery plaque
    Ryuichi Morishita
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Suita, Japan
    Curr Drug Targets 3:379-85. 2002
  2. ncbi request reprint Antisense oligodeoxynucleotide inhibition of vascular angiotensin-converting enzyme expression attenuates neointimal formation: evidence for tissue angiotensin-converting enzyme function
    R Morishita
    Division of Gene Therapy Science, Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Arterioscler Thromb Vasc Biol 20:915-22. 2000
  3. ncbi request reprint Oligonucleotide-based gene therapy for cardiovascular disease: are oligonucleotide therapeutics novel cardiovascular drugs?
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita 565, Japan
    Curr Drug Targets 1:15-23. 2000
  4. ncbi request reprint Apolipoprotein E-deficient mice created by systemic administration of antisense oligodeoxynucleotides: a new model for lipoprotein metabolism studies
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita 565 0871, Japan
    J Endocrinol 175:475-85. 2002
  5. ncbi request reprint [Gene therapy in cardiovascular medicine as new pharmacological therapy]
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan 2 2 Yamada oka, Suita, Osaka 565, Japan
    Nihon Yakurigaku Zasshi 120:222-8. 2002
  6. ncbi request reprint Decoy oligodeoxynucleotides as novel cardiovascular drugs for cardiovascular disease
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita, Japan
    Ann N Y Acad Sci 947:294-301; discussion 301-2. 2001
  7. ncbi request reprint Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy
    R Morishita
    Department of Geriatric Medicine, Osaka University Medical School, Suita 565, Japan
    Hypertension 33:1379-84. 1999
  8. ncbi request reprint Gene therapy in vascular medicine: recent advances and future perspectives
    R Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    Pharmacol Ther 91:105-14. 2001
  9. ncbi request reprint Recent progress in gene therapy for cardiovascular disease
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Circ J 66:1077-86. 2002
  10. ncbi request reprint Impairment of collateral formation in lipoprotein(a) transgenic mice: therapeutic angiogenesis induced by human hepatocyte growth factor gene
    Ryuichi Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan
    Circulation 105:1491-6. 2002

Collaborators

Detail Information

Publications114 found, 100 shown here

  1. ncbi request reprint HMG-Co A reductase inhibitors in the treatment of cardiovascular diseases: stabilization of coronary artery plaque
    Ryuichi Morishita
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Suita, Japan
    Curr Drug Targets 3:379-85. 2002
    ..This article reviews the direct action of statins on the blood vessel wall, with reference to classification of statins based on difference in action on the blood vessel wall (hepatic statins vs. vascular statins)...
  2. ncbi request reprint Antisense oligodeoxynucleotide inhibition of vascular angiotensin-converting enzyme expression attenuates neointimal formation: evidence for tissue angiotensin-converting enzyme function
    R Morishita
    Division of Gene Therapy Science, Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Arterioscler Thromb Vasc Biol 20:915-22. 2000
    ..The results support the existence of a functional tissue angiotensin system in the rat vessel wall...
  3. ncbi request reprint Oligonucleotide-based gene therapy for cardiovascular disease: are oligonucleotide therapeutics novel cardiovascular drugs?
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita 565, Japan
    Curr Drug Targets 1:15-23. 2000
    ..In this review, we have focused on the future potential of oligonucleotide (antisense, decoy & ribozyme)-based gene therapy for the treatment of cardiovascular disease...
  4. ncbi request reprint Apolipoprotein E-deficient mice created by systemic administration of antisense oligodeoxynucleotides: a new model for lipoprotein metabolism studies
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita 565 0871, Japan
    J Endocrinol 175:475-85. 2002
    ..Apolipoprotein-deficient mice created by intravenous administration of antisense ODN are a promising new animal model to help understand the role of apolipoprotein in vivo and develop a new drug therapy targeting apolipoprotein...
  5. ncbi request reprint [Gene therapy in cardiovascular medicine as new pharmacological therapy]
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan 2 2 Yamada oka, Suita, Osaka 565, Japan
    Nihon Yakurigaku Zasshi 120:222-8. 2002
    ..In this review, we have focused on the future potential of gene therapy for the treatment of cardiovascular disease as a new pharmacological therapy...
  6. ncbi request reprint Decoy oligodeoxynucleotides as novel cardiovascular drugs for cardiovascular disease
    R Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Suita, Japan
    Ann N Y Acad Sci 947:294-301; discussion 301-2. 2001
    ..In this review, we focus on the future potential of decoy oligodeoxynucleotide-based gene therapy in the treatment of cardiovascular disease...
  7. ncbi request reprint Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy
    R Morishita
    Department of Geriatric Medicine, Osaka University Medical School, Suita 565, Japan
    Hypertension 33:1379-84. 1999
    ..In the presence of decreased endogenous HGF, administration of rhHGF induced therapeutic angiogenesis in the rabbit ischemic hind limb model, as potential cytokine supplement therapy for peripheral arterial disease...
  8. ncbi request reprint Gene therapy in vascular medicine: recent advances and future perspectives
    R Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    Pharmacol Ther 91:105-14. 2001
    ..This review focuses on the future potential of gene therapy for the treatment of cardiovascular disease...
  9. ncbi request reprint Recent progress in gene therapy for cardiovascular disease
    Ryuichi Morishita
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Circ J 66:1077-86. 2002
    ..There is great potential in gene therapy for cardiovascular disease...
  10. ncbi request reprint Impairment of collateral formation in lipoprotein(a) transgenic mice: therapeutic angiogenesis induced by human hepatocyte growth factor gene
    Ryuichi Morishita
    Division of Gene Therapy Science, Osaka University Medical School, Osaka, Japan
    Circulation 105:1491-6. 2002
    ..In addition, we examined the feasibility of gene therapy by using an angiogenic growth factor, hepatocyte growth factor (HGF), to treat PAD in the presence of high Lp(a)...
  11. ncbi request reprint Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat diabetic hind limb ischemia model: molecular mechanisms of delayed angiogenesis in diabetes
    Y Taniyama
    Department of Geriatric Medicine, Division of Gene Therapy Science, Biomedical Research Center, Osaka University Medical School, Suita, Japan
    Circulation 104:2344-50. 2001
    ..The delay of angiogenesis in diabetes might be due to downregulation of MMP-1 and ets-1 through a decrease in HGF by high D-glucose...
  12. ncbi request reprint Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease
    Y Taniyama
    Department of Geriatric Medicine, Biomedical Research Center, Osaka University Medical School, Japan
    Gene Ther 8:181-9. 2001
    ..01). Overall, intramuscular injection of naked human HGF plasmid induced therapeutic angiogenesis in rat and rabbit ischemic hindlimb models, as potential therapy for peripheral arterial disease...
  13. ncbi request reprint Inhibition of TNF-alpha, induced cytokine and adhesion molecule. Expression in glomerular cells in vitro and in vivo by transcription factor decoy for NFkappaB
    N Tomita
    Department of General Medicine, Osaka University Hospital, Suita, Japan
    Exp Nephrol 9:181-90. 2001
    ....
  14. ncbi request reprint Inhibition of neointima by angiotensin-converting enzyme inhibitor in porcine coronary artery balloon-injury model
    K Matsumoto
    Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan
    Hypertension 37:270-4. 2001
    ..Downregulation of the local vascular HGF system may play an important role in the pathogenesis of cardiovascular disease...
  15. ncbi request reprint Mitogenic and antiapoptotic actions of hepatocyte growth factor through ERK, STAT3, and AKT in endothelial cells
    H Nakagami
    Department of Geriatric Medicine, Division of Gene Therapy Science, Osaka University, Osaka, Japan
    Hypertension 37:581-6. 2001
    ..These findings provide new perspectives in the role of HGF in cardiovascular disease...
  16. ncbi request reprint Prevention of abdominal aortic aneurysms by simultaneous inhibition of NFkappaB and ets using chimeric decoy oligonucleotides in a rabbit model
    T Miyake
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Gene Ther 13:695-704. 2006
    ..Further modification of chimeric decoy ODN would be useful to treat AAA as a decoy-based therapy...
  17. ncbi request reprint Gene transfer of dominant-negative mutants of extracellular signal-regulated kinase and c-Jun NH2-terminal kinase prevents neointimal formation in balloon-injured rat artery
    Y Izumi
    Department of Pharmacology, Osaka City University Medical School, Osaka, Japan
    Circ Res 88:1120-6. 2001
    ..Thus, ERK and JNK activation triggers SMC proliferation, leading to neointimal formation. These kinases may be the new therapeutic targets for prevention of vascular diseases...
  18. ncbi request reprint Inhibition of NFkappaB activation using cis-element 'decoy' of NFkappaB binding site reduces neointimal formation in porcine balloon-injured coronary artery model
    K Yamasaki
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Gene Ther 10:356-64. 2003
    ..Here, we report the successful in vivo transfer of NFkappaB decoy ODN using a hydrogel catheter to inhibit vascular lesion formation in balloon-injured porcine coronary artery...
  19. ncbi request reprint [Gene therapy for arteriosclerotic diseases]
    M Aoki
    Department of Geriatric Medicine, Osaka University Medical School
    Nippon Rinsho 59:43-52. 2001
    ..We focus on HGF, which is a novel endothelial growth factor. HGF-based therapeutic angiogenesis would possibly result a new treatment for severe ischemic diseases...
  20. ncbi request reprint HGF/NK4 inhibited VEGF-induced angiogenesis in in vitro cultured endothelial cells and in vivo rabbit model
    M Nakabayashi
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Diabetologia 46:115-23. 2003
    ..Our aim was to explore the inhibitory effects of HGF/NK4 on angiogenesis induced by VEGF...
  21. ncbi request reprint Phosphorylation of p38 mitogen-activated protein kinase downstream of bax-caspase-3 pathway leads to cell death induced by high D-glucose in human endothelial cells
    H Nakagami
    Department of Geriatric Medicine, Osaka University Medical School, Yamada-oka, Suita, Osaka, Japan
    Diabetes 50:1472-81. 2001
    ..Phosphorylation of p38 MAPK downstream of the bax-caspase pathway may play a pivotal role in endothelial apoptosis mediated by high D-glucose...
  22. ncbi request reprint Inhibition of neointimal formation after balloon injury by cilostazol, accompanied by improvement of endothelial dysfunction and induction of hepatocyte growth factor in rat diabetes model
    M Aoki
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
    Diabetologia 44:1034-42. 2001
    ....
  23. ncbi request reprint Contribution of Bcl-2, but not Bcl-xL and Bax, to antiapoptotic actions of hepatocyte growth factor in hypoxia-conditioned human endothelial cells
    K Yamamoto
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Hypertension 37:1341-8. 2001
    ..The antiapoptotic mechanisms of HGF in hypoxia-induced endothelial cell death largely depend on Bcl-2, but not Bcl-xL and BAX:..
  24. ncbi request reprint Development of safe and efficient novel nonviral gene transfer using ultrasound: enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle
    Y Taniyama
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan
    Gene Ther 9:372-80. 2002
    ..Transfection of HGF plasmid by the ultrasound-Optison method could be useful for safe clinical gene therapy to treat peripheral arterial disease without a viral vector system...
  25. ncbi request reprint Acceleration of wound healing by combined gene transfer of hepatocyte growth factor and prostacyclin synthase with Shima Jet
    Y Kunugiza
    1 1Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Suita, Japan 2 2Department of Orthopedic Surgery, Osaka University Graduate School of Medicine, Suita, Japan
    Gene Ther 13:1143-52. 2006
    ..These results indicate that co-transfer of HGF and PGIS genes by the Shima Jet could be an effective strategy to wound healing...
  26. ncbi request reprint Enhancement of transgene expression by cotransfection of oriP plasmid with EBNA-1 expression vector
    Y Kaneda
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Hum Gene Ther 11:471-9. 2000
    ..Thus, cotransfection of oriP-harboring expression vector with EBNA-1 expression plasmid should be promising for human gene therapy, although the safety of the system must be investigated thoroughly...
  27. ncbi request reprint Angiogenic and antifibrotic actions of hepatocyte growth factor improve cardiac dysfunction in porcine ischemic cardiomyopathy
    J Azuma
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Gene Ther 13:1206-13. 2006
    ..These favorable outcomes suggest potential utility to treat patients with ischemic heart disease using HGF gene transfer. Currently, a phase I study using human HGF plasmid DNA is ongoing to test the validity of this concept...
  28. ncbi request reprint [HGF as a key molecule in cardiovascular diseases]
    M Aoki
    Department of Geriatric Medicine, Osaka University Medical School
    Nippon Rinsho 59:2460-9. 2001
    ..In near future, powerful therapeutic effects of HGF could applied to restenosis, graft failure, cardiomyopathy, cerebral vascular diseases, renal failure and so on...
  29. ncbi request reprint Impaired endothelial dysfunction in diabetes mellitus rats was restored by oral administration of prostaglandin I2 analogue
    K Matsumoto
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Japan
    J Endocrinol 175:217-23. 2002
    ..Increased local vascular HGF production by a PGI(2) analogue may prevent endothelial injury, potentially resulting in the improvement of endothelial dysfunction...
  30. ncbi request reprint Safety evaluation of hemagglutinating virus of Japan--artificial viral envelope liposomes in nonhuman primates
    N Tsuboniwa
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
    Hum Gene Ther 12:469-87. 2001
    ..Moreover, no histological changes were observed. This safety evaluation study demonstrates the safety, feasibility, and therapeutic potential of the novel transfection vehicle, HVJ--AVE liposomes, in humans...
  31. ncbi request reprint Prevention of onset of Parkinson's disease by in vivo gene transfer of human hepatocyte growth factor in rodent model: a model of gene therapy for Parkinson's disease
    H Koike
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Gene Ther 13:1639-44. 2006
    ..Overall, the present study demonstrated that overexpression of HGF prevented neuronal death in a PD rat model, providing a potential novel therapy for PD...
  32. ncbi request reprint Intravenous injection of oligodeoxynucleotides to the NF-kappaB binding site inhibits hepatic metastasis of M5076 reticulosarcoma in mice
    I Kawamura
    Medicinal Biology Research Laboratories, Fujisawa Pharmaceutical Co, Ltd, Osaka, Japan
    Gene Ther 8:905-12. 2001
    ....
  33. ncbi request reprint Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound
    M Shimamura
    Division of Clinical Gene Therapy, Osaka University, Yamada oka, Suita, Japan
    Gene Ther 11:1532-9. 2004
    ..Overall, the present study demonstrated the feasibility of efficient plasmid DNA transfer into the central nervous system, providing a new option for treating various diseases such as tumors...
  34. ncbi request reprint NFkappaB decoy oligodeoxynucleotides ameliorates osteoporosis through inhibition of activation and differentiation of osteoclasts
    H Shimizu
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Osaka, Japan
    Gene Ther 13:933-41. 2006
    ..Overall, inhibition of NFkappaB by decoy strategy prevented osteoporosis through the inhibition of bone resorption. Targeting of NFkappaB might be potential therapy in various bone metabolic diseases...
  35. ncbi request reprint Transfection of antisense p53 tumor suppressor gene oligodeoxynucleotides into rat carotid artery results in abnormal growth of vascular smooth muscle cells
    H Matsushita
    Department of Geriatric Medicine, Osaka University Medical School, Suita 565, Japan
    Circulation 101:1447-52. 2000
    ..In this study, to clarify the role of p53 in the pathogenesis of restenosis, we examined transfection of antisense p53 oligodeoxynucleotides (ODN) into VSMC in vitro and rat carotid artery in vivo...
  36. ncbi request reprint Endothelial apoptosis induced by oxidative stress through activation of NF-kappaB: antiapoptotic effect of antioxidant agents on endothelial cells
    M Aoki
    Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan
    Hypertension 38:48-55. 2001
    ..Oxidative stress may play an important role in endothelial apoptosis mediated by hypoxia, through the activation of NF-kappaB...
  37. doi request reprint Alleviation of Abeta-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model
    D Takeuchi
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Japan
    Gene Ther 15:561-71. 2008
    ..A pharmacological approach including gene therapy to increase the HGF level in combination with anti-Abeta therapy might be a new therapeutic option for the treatment of AD...
  38. ncbi request reprint Symposium on the etiology of hypertension--summarizing studies in 20th century. 1. Hypertension and genes
    J Higaki
    The Department of Geriatric Medicine, Osaka University Medical School, Suita
    Intern Med 40:144-7. 2001
    ..Therefore, it is not extraordinary for unexpected results to be obtained in the correlation between a gene and a disease, suggesting that this approach has a possibility to be a breakthrough in the chaos of hypertension research...
  39. ncbi request reprint EBV replicon vector system enhances transgene expression in vivo: applications to cancer gene therapy
    T Otomo
    Division of Gene Therapy Science, Osaka University School of Medicine, Suita, Japan
    J Gene Med 3:345-52. 2001
    ..CONCLUSION: The EBV replicon vector appears useful for cancer gene therapy. Analysis of the transgene in tumors suggests that the EBV replicon system may be responsible for efficient transcription but not retention of the transgene...
  40. ncbi request reprint Transfection of NFkappaB-decoy oligodeoxynucleotides using efficient ultrasound-mediated gene transfer into donor kidneys prolonged survival of rat renal allografts
    H Azuma
    Department of Urology, Osaka Medical College, Takatsuki, Osaka, Japan
    Gene Ther 10:415-25. 2003
    ....
  41. ncbi request reprint Inhibition of ets, an essential transcription factor for angiogenesis, to prevent the development of abdominal aortic aneurysm in a rat model
    K Miwa
    Division of Clinical Gene Therapy, Osaka University, Suita, Osaka, Japan
    Gene Ther 12:1109-18. 2005
    ..Ets might be a potential target to develop pharmacotherapy/gene therapy to treat AAA through the inhibition of MMPs...
  42. ncbi request reprint Effect of nifedipine on endothelial function in normotensive smokers: potential contribution of increase in circulating hepatocyte growth factor
    K Yamasaki
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan
    J Hum Hypertens 18:701-5. 2004
    ..The increase in serum HGF concentration by nifedipine might contribute to the improvement of endothelial dysfunction...
  43. ncbi request reprint Gene therapy using HVJ-liposomes: the best of both worlds?
    Y Kaneda
    Division of Gene Therapy Science, Osaka University School of Medicine, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Mol Med Today 5:298-303. 1999
    ..In animal models, this system has shown promise for several diseases, including cancer and cardiovascular disease...
  44. ncbi request reprint Cold shock domain protein A represses angiogenesis and lymphangiogenesis via inhibition of serum response element
    Y Saito
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka, Japan
    Oncogene 27:1821-33. 2008
    ..In conclusion, these data suggest that expression of CSDA can repress angiogenesis and lymphangiogenesis via direct binding to SRE in addition to HRE...
  45. ncbi request reprint Dominant negative c-jun gene transfer inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in rats
    H Yasumoto
    Department of Pharmacology, Osaka City University Medical School, Osaka, Japan
    Gene Ther 8:1682-9. 2001
    ..Thus, AP-1 seems to be the new therapeutic target for treatment of vascular diseases...
  46. ncbi request reprint Molecular strategy using cis-element 'decoy' of E2F binding site inhibits neointimal formation in porcine balloon-injured coronary artery model
    T Nakamura
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Gene Ther 9:488-94. 2002
    ..Here, we report the successful in vivo transfer of E2F decoy ODN using a hydrogel catheter to inhibit vascular lesion formation in balloon-injured porcine coronary artery without any apparent side-effects...
  47. ncbi request reprint Prevention and regression of atopic dermatitis by ointment containing NF-kB decoy oligodeoxynucleotides in NC/Nga atopic mouse model
    H Nakamura
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan
    Gene Ther 9:1221-9. 2002
    ..Here, we demonstrated the successful treatment of atopic dermatitis with ointment containing NF-kB decoy ODN in a mouse model, promising new therapy for atopic dermatitis...
  48. ncbi request reprint Expression of local hepatocyte growth factor system in vascular tissues
    Y Nakamura
    Department of Geriatric Medicine, Osaka University Medical School, Japan
    Biochem Biophys Res Commun 215:483-8. 1995
    ..Since HGF has characteristics of an endothelium-specific growth factor, locally synthesized HGF in endothelial cells and VSMC may have a role in vascular functions in autocrine-paracrine manner...
  49. ncbi request reprint In vivo evaluation of in vitro hypothesis using "gain or loss" of function: functional analysis of renin-angiotensin system
    R Morishita
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Am J Hypertens 11:507-13. 1998
    ..Such an approach may increase our understanding of the biology and pathobiology of the autocrine-paracrine system in hypertension. This review discusses the potential utility of in vivo gene transfer methods...
  50. ncbi request reprint Functional analysis of cardiovascular renin-angiotensin system using a gain or loss of function approach
    R Morishita
    Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan
    Hypertens Res 23:137-41. 2000
    ..Such an approach may increase our understanding of the biology and pathobiology of the autocrine-paracrine system. This review discusses the potential utility of in vivo gene transfer methods...
  51. ncbi request reprint Antisense oligonucleotides as a powerful molecular strategy for gene therapy in cardiovascular diseases
    N Tomita
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, 2 2 Yamada0oka, Suita 565 0871, Japan
    Curr Pharm Des 10:797-803. 2004
    ..In this review we would like to introduce some modifications of ODN design and examples of our applications of antisense ODN in cardiovascular disease in animal models...
  52. ncbi request reprint NF-kappa B decoy suppresses cytokine expression and thermal hyperalgesia in a rat neuropathic pain model
    G Sakaue
    Department of Anesthesiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
    Neuroreport 12:2079-84. 2001
    ..This finding suggests that a perineural inflammatory cascade, that involves NF-kappaB, is involved in the pathogenesis of neuropathic pain...
  53. ncbi request reprint Novel immunotherapy for peritoneal dissemination of murine colon cancer with macrophage inflammatory protein-1beta mediated by a tumor-specific vector, HVJ cationic liposomes
    T Miyata
    Division of Gene Therapy Science, Osaka University School of Medicine, Suita, Osaka, Japan
    Cancer Gene Ther 8:852-60. 2001
    ..Therefore, this HVJ cationic liposome strategy may serve as a powerful tool against peritoneal disseminated cancer...
  54. ncbi request reprint Transcription factor decoy to study the molecular mechanism of negative regulation of renin gene expression in the liver in vivo
    S Tomita
    Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women s Hospital, Harvard University Medical School, Boston, MA, USA
    Circ Res 84:1059-66. 1999
    ..Taken together, these results document the importance of NREB in the inhibition of renin gene expression in rat liver in vivo and suggest the possibility of in vivo renin gene modulation by the TFD approach...
  55. doi request reprint Novel microdialysis method to assess neuropeptides and large molecules in free-moving mouse
    S Takeda
    Department of Clinical Gene Therapy, Osaka University, Yamadaoka, Osaka 565 0871, Japan
    Neuroscience 186:110-9. 2011
    ....
  56. ncbi request reprint Nuclear factor-kappa B decoy attenuates neuronal damage after global brain ischemia: a future strategy for brain protection during circulatory arrest
    T Ueno
    Division of Cardiovascular Surgery, Department of Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    J Thorac Cardiovasc Surg 122:720-7. 2001
    ..CONCLUSIONS: Therapeutic transfection of nuclear factor-kappa B decoy oligodeoxynucleotides during brain ischemia may be useful for attenuating neuronal damage, suggesting a strategy for cerebral protection against global ischemia...
  57. ncbi request reprint Magnetic nanoparticles with surface modification enhanced gene delivery of HVJ-E vector
    Norio Morishita
    Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan
    Biochem Biophys Res Commun 334:1121-6. 2005
    ..Overall, magnetic nanoparticles with different surface modifications can enhance HVJ-E-based gene transfer by modification of the size or charge, which could potentially help to overcome fundamental limitations to gene therapy in vivo...
  58. ncbi request reprint Hepatocyte growth factor gene therapy reduces ventricular arrhythmia in animal models of myocardial ischemia
    Akihisa Yumoto
    Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Acta Med Okayama 59:73-8. 2005
    ..Histological analyses revealed that the number of vessels in the ischemic border zone was greatly increased after HGF gene injection. These findings revealed that HGF gene therapy has an anti-arrhythmic effect after myocardial ischemia...
  59. ncbi request reprint Development of novel method of non-viral efficient gene transfer into neonatal cardiac myocytes
    Hironori Tashiro
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Japan
    J Mol Cell Cardiol 39:503-9. 2005
    ....
  60. ncbi request reprint Nonviral gene transfer of human hepatocyte growth factor improves streptozotocin-induced diabetic neuropathy in rats
    Naoki Kato
    Department of Orthopaedic Surgery, National Defense Medical College, 3 2 Namiki, Tokorozawa, Saitama, Japan 359 8513
    Diabetes 54:846-54. 2005
    ..These results suggest that nonviral HVJ liposome-mediated gene transfer of human HGF has potential for the safe effective treatment of diabetic sensorimotor neuropathy...
  61. ncbi request reprint [Gene therapy for diabetic microangiopathy]
    Yoshiaki Taniyama
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine
    Nihon Rinsho 63:153-8. 2005
  62. ncbi request reprint An efficient gene transfer method mediated by ultrasound and microbubbles into the kidney
    Hiromi Koike
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Suita 565 0871, Japan
    J Gene Med 7:108-16. 2005
    ..From this point of view, it would be better to develop a novel non-viral efficient gene transfer method. Recently, it was reported that ultrasound exposure could induce cell membrane permeabilization and enhance gene expression...
  63. ncbi request reprint Gene transfer into adult rat spinal cord using naked plasmid DNA and ultrasound microbubbles
    Munehisa Shimamura
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Yamada oka 2 2, Suita, Osaka 565 0871, Japan
    J Gene Med 7:1468-74. 2005
    ....
  64. ncbi request reprint Therapeutic angiogenesis for critical limb ischemia: design of the hepatocyte growth factor therapeutic angiogenesis clinical trial
    Richard J Powell
    Division of Vascular Surgery, Department of Surgery, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
    Vasc Med 9:193-8. 2004
    ..In conclusion, this study will help to determine whether therapeutic angiogenesis with HGF is a viable option in the treatment of patients with CLI...
  65. ncbi request reprint HVJ-based non-viral gene transfer method: successful gene therapy using HGF and VEGF genes in experimental ischemia
    Munehisa Shimamura
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Japan
    Front Biosci 11:753-9. 2006
    ..This review discusses the feasibility of gene therapy using an HVJ-based non-viral vector containing the VEGF or HGF gene for cerebral ischemia...
  66. ncbi request reprint Ultrasound-microbubble-mediated NF-kappaB decoy transfection attenuates neointimal formation after arterial injury in mice
    Hiroshi Inagaki
    Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    J Vasc Res 43:12-8. 2006
    ..Decoy transfection is a significant methodology for suppressing gene activation. The decoy can be synthesized easily; however, the major obstacle is the difficulty involved in effective in vivo delivery...
  67. doi request reprint Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model
    Takashi Miyake
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Cardiovasc Res 79:706-14. 2008
    ..The purpose of this study was to determine the effects of simultaneous inhibition of these transcription factors on the formation of neointimal hyperplasia...
  68. ncbi request reprint Marked regression of liver metastasis by combined therapy of ultrasound-mediated NF kappaB-decoy transfer and transportal injection of paclitaxel, in mouse
    Haruhito Azuma
    Department of Urology, Osaka Medical College, Takatsuki, Osaka, Japan
    Int J Cancer 122:1645-56. 2008
    ..In conclusion, a combination of local NF kappaB-decoy transfer into the liver and transportal injection of paclitaxel may be a safe and effective new therapy for liver metastasis...
  69. ncbi request reprint Involvement of gamma-secretase in postnatal angiogenesis
    Hiroki Hayashi
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 363:584-90. 2007
    ....
  70. ncbi request reprint Novel mechanisms of valsartan on the treatment of acute myocardial infarction through inhibition of the antiadhesion molecule periostin
    Kazuma Iekushi
    Department of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertension 49:1409-14. 2007
    ..The inhibition of periostin by valsartan might especially contribute to its beneficial effects on cardiac remodeling after myocardial infarction...
  71. ncbi request reprint Transfection of human hepatocyte growth factor gene ameliorates secondary lymphedema via promotion of lymphangiogenesis
    Yukihiro Saito
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Circulation 114:1177-84. 2006
    ..Treatment for this disabling condition remains limited and largely ineffective. The goal of the present study was to investigate the therapeutic efficacy of hepatocyte growth factor (HGF) in animal models of lymphedema...
  72. ncbi request reprint Nonviral HVJ (hemagglutinating virus of Japan) liposome-mediated retrograde gene transfer of human hepatocyte growth factor into rat nervous system promotes functional and histological recovery of the crushed nerve
    Naoki Kato
    Department of Orthopaedic Surgery, National Defense Medical College, 3 2 Namiki, Tokorozawa, Saitama 359 8513, Japan
    Neurosci Res 52:299-310. 2005
    ....
  73. ncbi request reprint Hypertension accelerated experimental abdominal aortic aneurysm through upregulation of nuclear factor kappaB and Ets
    Suguru Shiraya
    Division of Clinical Gene Therapy, Graduate School of Medicine, Osaka University, Suita, Japan
    Hypertension 48:628-36. 2006
    ..Inhibition of NFkappaB and ets could be a novel therapeutic strategy to treat AAA in hypertensive patients...
  74. ncbi request reprint Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy
    Hironori Nakagami
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    J Atheroscler Thromb 13:77-81. 2006
    ..These adipose tissue-derived cells have potential in angiogenic cell therapy for ischemic disease, and might be applied for regenerative cell therapy instead of bone marrow cells in the near future...
  75. ncbi request reprint [Therapeutic angiogenesis for ischemic diseases]
    Motokuni Aoki
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine
    Nihon Rinsho 64:762-8. 2006
    ..Therapeutic angiogenesis using angiogenic growth factors is expected to be a new treatment for patients with severe ischemic heart or peripheral arterial disease...
  76. ncbi request reprint [Gene therapy in congestive heart failure]
    Yoshiaki Taniyama
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine
    Nihon Rinsho 63:594-7. 2005
  77. ncbi request reprint [Therapeutic angiogenesis for ischemic heart disease induced by gene transfer]
    Yoshiaki Taniyama
    Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine
    Nihon Rinsho 63:588-93. 2005
  78. ncbi request reprint [Gene therapy for peripheral arterial disease]
    Hirofumi Makino
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine
    Nihon Rinsho 63:582-7. 2005
  79. doi request reprint Results of a double-blind, placebo-controlled study to assess the safety of intramuscular injection of hepatocyte growth factor plasmid to improve limb perfusion in patients with critical limb ischemia
    Richard J Powell
    Department of Surgery, Section of Vascular Surgery, Dartmouth Hitchcock Medical Center, One Medical Center Dr, Lebanon, NH 03756, USA
    Circulation 118:58-65. 2008
    ....
  80. ncbi request reprint Enhanced tumor-specific long-term immunity of hemagglutinating [correction of hemaggluttinating] virus of Japan-mediated dendritic cell-tumor fused cell vaccination by coadministration with CpG oligodeoxynucleotides
    Kazuya Hiraoka
    Division of Gene Therapy Science, Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita, Japan
    J Immunol 173:4297-307. 2004
    ..Thus, the combination of DC-tumor FCs and CpG ODN is an effective and feasible cancer vaccine to prevent the generation and recurrence of cancers...
  81. ncbi request reprint Periostin as a novel factor responsible for ventricular dilation
    Naruto Katsuragi
    Daiichi Suntory Biomedical Research Ltd, 1 1 1 Wakayamadai Shimamoto cho, Mishima gun, Osaka, Japan
    Circulation 110:1806-13. 2004
    ..To identify the function of periostin in the pathophysiology of heart failure, overexpression or loss of function of the periostin gene was examined by direct transfection into the rat heart...
  82. ncbi request reprint [Gene therapy for restenosis after angioplasty]
    Motokuni Aoki
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine
    Nihon Rinsho 61:685-91. 2003
  83. ncbi request reprint Cardiovascular disease and angiogenesis
    Ryuichi Morishita
    Department of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita
    Intern Med 42:301-2. 2003
  84. ncbi request reprint Efficient gene transfer from innervated muscle into rat peripheral and central nervous systems using a non-viral haemagglutinating virus of Japan (HVJ)-liposome method
    Naoki Kato
    Department of Orthopaedic Surgery, National Defense Medical College Research Institute, Saitama, Japan
    J Neurochem 85:810-5. 2003
    ....
  85. ncbi request reprint Enhanced angiogenesis and improvement of neuropathy by cotransfection of human hepatocyte growth factor and prostacyclin synthase gene
    Hiromi Koike
    Division of Gene Therapy Science, Osaka University Medical School, Suita 565, Japan
    FASEB J 17:779-81. 2003
    ..These data provide important information relating to the clinical application of therapeutic angiogenesis to treat peripheral arterial disease...
  86. ncbi request reprint HVJ-envelope vector for gene transfer into central nervous system
    Munehisa Shimamura
    Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita 565 0871, Osaka, Japan
    Biochem Biophys Res Commun 300:464-71. 2003
    ..Finally, luciferase activity was markedly enhanced by the addition of 50 U/ml heparin (P<0.01). Development of efficient HVJ-E for gene transfer into the CNS will be useful for research and clinical gene therapy...
  87. ncbi request reprint [Gene therapy for peripheral arterial diseases in diabetes mellitus]
    Hirofumi Makino
    Department of Geriatric Medicine, Osaka University Medical School
    Nihon Rinsho 60:449-56. 2002
  88. pmc Gene transfer of human hepatocyte growth factor into rat skin wounds mediated by liposomes coated with the sendai virus (hemagglutinating virus of Japan)
    Kuniaki Nakanishi
    Division of Environmental Medicine, National Defense Medical College, Tokorozawa, Japan
    Am J Pathol 161:1761-72. 2002
    ..These results suggest that HGF gene transfer into a skin wound may aid re-epithelialization and neovascularization in the early phase of wound healing, and that HGF may play a role in modulating cutaneous wound healing...
  89. ncbi request reprint [The latest review of therapeutic angiogenesis using angiogenic growth factors to peripheral arterial diseases]
    Motokuni Aoki
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine
    Nihon Rinsho 60:1845-55. 2002
    ..In a prospective, open-labeled clinical trial, we investigated the safety and biological efficiency of this gene therapy in patients with peripheral arterial disease(PAD) who had failed conventional therapy...
  90. ncbi request reprint Hepatocyte growth factor as cardiovascular hormone: role of HGF in the pathogenesis of cardiovascular disease
    Ryuichi Morishita
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Endocr J 49:273-84. 2002
    ..In this review, we discussed the potential role of HGF in cardiovascular disease...
  91. ncbi request reprint Angiogenesis and antifibrotic action by hepatocyte growth factor in cardiomyopathy
    Yoshiaki Taniyama
    Department of Geriatric Medicine, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Hypertension 40:47-53. 2002
    ..These results suggest that HGF gene transfer may be useful to protect against myocardial injury in cardiomyopathy through its cardioprotective effects such as antifibrosis and angiogenesis actions...
  92. ncbi request reprint Inhibitory effects of novel AP-1 decoy oligodeoxynucleotides on vascular smooth muscle cell proliferation in vitro and neointimal formation in vivo
    Jong Deok Ahn
    Department of Microbiology, Kyungpook National University, Taegu, Korea
    Circ Res 90:1325-32. 2002
    ..Moreover, the use of stable CDODN specific for AP-1 activity in combination with the highly effective HVJ-liposome method provides a novel potential therapeutic strategy for the prevention of restenosis after angioplasty in humans...
  93. ncbi request reprint Gene transfer of hepatocyte growth factor to subarachnoid space in cerebral hypoperfusion model
    Shin ichi Yoshimura
    Department of Neurosurgery, Gifu University School of Medicine, Gifu, Japan
    Hypertension 39:1028-34. 2002
    ..01). Here, we demonstrated successful therapeutic angiogenesis using HGF or VEGF gene transfer into the subarachnoid space to improve cerebral hypoperfusion, thus providing a new therapeutic strategy for cerebral ischemic disease...
  94. ncbi request reprint Fetal gene transfer by intrauterine injection with microbubble-enhanced ultrasound
    Masayuki Endoh
    Department of Gene Therapy Science, Osaka University Medical School, Yamada oka 2 2, Suita, Japan
    Mol Ther 5:501-8. 2002
    ..Thus, SGM might provide a useful means to clarify the molecular mechanisms of genetic diseases in utero, as well as a tool to develop gene therapies in utero...
  95. ncbi request reprint Local delivery of plasmid DNA into rat carotid artery using ultrasound
    Yoshiaki Taniyama
    Department of Geriatric Medicine, Osaka University, Graduate School of Medicine, Osaka, Japan
    Circulation 105:1233-9. 2002
    ..In this study, we examined the feasibility of a novel nonviral vector system by using high-frequency, low-intensity ultrasound irradiation for transfection into blood vessels...
  96. ncbi request reprint [Therapeutic angiogenesis by angiogenic growth factor]
    Hirofumi Makino
    Department of Geriatric Medicine, Osaka University Medical School
    Nihon Rinsho 61:692-7. 2003
  97. ncbi request reprint Inhibition of renal fibrosis by gene transfer of inducible Smad7 using ultrasound-microbubble system in rat UUO model
    Hui Y Lan
    Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    J Am Soc Nephrol 14:1535-48. 2003
    ..TGF-beta-mediated renal fibrosis is regulated positively by Smad2/3, but negatively by Smad7. Target blockade of TGF-beta/Smad signaling by expression of Smad7 may provide a new therapeutic potential for renal fibrosis...
  98. ncbi request reprint Nuclear factor kappa B decoy oligodeoxynucleotides prevent endotoxin-induced fatal liver failure in a murine model
    Ichiro Ogushi
    First Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan
    Hepatology 38:335-44. 2003
    ..In conclusion, NF-kappa B/decoy/ODN transfer in vivo effectively suppressed endotoxin-induced fatal liver injury in mice...
  99. ncbi request reprint Is vascular endothelial growth factor a missing link between hypertension and inflammation?
    Ryuichi Morishita
    Hypertension 44:253-4. 2004
  100. ncbi request reprint Lack of association between the hepatocyte growth factor receptor, c-met, and the anti-apoptotic action of bag-1 in endothelial cells
    Hironori Nakagami
    Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Japan
    Hypertens Res 27:359-65. 2004
    ..Taken together, these results indicate that over-expression of bag-1 has an anti-apoptotic effect on endothelial cells independent of HGF signaling...