Makoto Miyata

Summary

Affiliation: Osaka City University
Country: Japan

Publications

  1. pmc Isolation and characterization of P1 adhesin, a leg protein of the gliding bacterium Mycoplasma pneumoniae
    Daisuke Nakane
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 193:715-22. 2011
  2. pmc Force and velocity of mycoplasma mobile gliding
    Makoto Miyata
    Rowland Institute for Science, Cambridge, Massachusetts 02142, USA
    J Bacteriol 184:1827-31. 2002
  3. ncbi request reprint [Gliding motility of mycoplasmas, a novel mechanism of biological motility]
    Makoto Miyata
    Tanpakushitsu Kakusan Koso 50:239-45. 2005
  4. ncbi request reprint Cytoskeleton of mollicutes
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Mol Microbiol Biotechnol 11:256-64. 2006
  5. ncbi request reprint Asymmetrical progression of replication forks just after initiation on Mycoplasma capricolum chromosome revealed by two-dimensional gel electrophoresis
    M Miyata
    Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi ku, Japan
    Gene 193:39-47. 1997
  6. ncbi request reprint Gliding mutants of Mycoplasma mobile: relationships between motility and cell morphology, cell adhesion and microcolony formation
    M Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Microbiology 146:1311-20. 2000
  7. pmc Spike structure at the interface between gliding Mycoplasma mobile cells and glass surfaces visualized by rapid-freeze-and-fracture electron microscopy
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 186:4382-6. 2004
  8. doi request reprint Unique centipede mechanism of Mycoplasma gliding
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Annu Rev Microbiol 64:519-37. 2010
  9. ncbi request reprint Movement on the cell surface of the gliding bacterium, Mycoplasma mobile, is limited to its head-like structure
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Japan
    FEMS Microbiol Lett 215:285-9. 2002
  10. ncbi request reprint Centipede and inchworm models to explain Mycoplasma gliding
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Japan
    Trends Microbiol 16:6-12. 2008

Collaborators

Detail Information

Publications41

  1. pmc Isolation and characterization of P1 adhesin, a leg protein of the gliding bacterium Mycoplasma pneumoniae
    Daisuke Nakane
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 193:715-22. 2011
    ..Sequence analysis of P1 adhesin and its orthologs showed that domain I is well conserved and that a transmembrane segment exists near the link between domains II and III...
  2. pmc Force and velocity of mycoplasma mobile gliding
    Makoto Miyata
    Rowland Institute for Science, Cambridge, Massachusetts 02142, USA
    J Bacteriol 184:1827-31. 2002
    ..Other implications of these results are discussed...
  3. ncbi request reprint [Gliding motility of mycoplasmas, a novel mechanism of biological motility]
    Makoto Miyata
    Tanpakushitsu Kakusan Koso 50:239-45. 2005
  4. ncbi request reprint Cytoskeleton of mollicutes
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Mol Microbiol Biotechnol 11:256-64. 2006
    ..Mollicutes have special cytoskeletal proteins and structures that are sometimes not shared even by other mollicute species...
  5. ncbi request reprint Asymmetrical progression of replication forks just after initiation on Mycoplasma capricolum chromosome revealed by two-dimensional gel electrophoresis
    M Miyata
    Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi ku, Japan
    Gene 193:39-47. 1997
    ..The results of N/A analysis of the 5.6-kb region showed a shift of intensity in the nascent strand signal, which suggests an upshift of fork progression velocity...
  6. ncbi request reprint Gliding mutants of Mycoplasma mobile: relationships between motility and cell morphology, cell adhesion and microcolony formation
    M Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Microbiology 146:1311-20. 2000
    ..Finally, the ability to form microcolonies on surfaces was shown to correlate with the gliding activity, suggesting a certain role of gliding motility in the parasitic life-cycle of mycoplasmas...
  7. pmc Spike structure at the interface between gliding Mycoplasma mobile cells and glass surfaces visualized by rapid-freeze-and-fracture electron microscopy
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 186:4382-6. 2004
    ..The spikes were on average 50 nm in length and 4 nm in diameter, most abundant around the head, and not observed in a nonbinding mutant. The spikes may be involved in the mechanism of binding, gliding, or both...
  8. doi request reprint Unique centipede mechanism of Mycoplasma gliding
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Annu Rev Microbiol 64:519-37. 2010
    ..The gliding of Mycoplasma pneumoniae, a species distantly related to M. mobile, is also discussed...
  9. ncbi request reprint Movement on the cell surface of the gliding bacterium, Mycoplasma mobile, is limited to its head-like structure
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Japan
    FEMS Microbiol Lett 215:285-9. 2002
    ..These results indicate that the head-like structure drags the cell body, leading us to conclude that the force for gliding is generated at the head-like structure...
  10. ncbi request reprint Centipede and inchworm models to explain Mycoplasma gliding
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Japan
    Trends Microbiol 16:6-12. 2008
    ..Another 'inchworm model' suggested from the structural studies of a human pathogen, M. pneumoniae, is also discussed...
  11. pmc Regions on Gli349 and Gli521 protein molecules directly involved in movements of Mycoplasma mobile gliding machinery, suggested by use of inhibitory antibodies and mutants
    Atsuko Uenoyama
    Department of Biology, Graduate School of Science, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 191:1982-5. 2009
    ....
  12. pmc Cytoskeletal "jellyfish" structure of Mycoplasma mobile
    Daisuke Nakane
    Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Proc Natl Acad Sci U S A 104:19518-23. 2007
    ..Immunofluorescence and immunoelectron microscopy revealed that two components are localized at the bell and another that has the structure similar to the F(1)-ATPase beta subunit is localized at the tentacles...
  13. pmc Identification of a 349-kilodalton protein (Gli349) responsible for cytadherence and glass binding during gliding of Mycoplasma mobile
    Atsuko Uenoyama
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585 PRESTO, JST, Osaka, Japan
    J Bacteriol 186:1537-45. 2004
    ..This suggested that the Gli349 molecule is involved not only in glass binding for gliding but also in movement. To explain the present results, a model for the mechanical cycle of gliding is discussed...
  14. ncbi request reprint Cell surface differentiation of Mycoplasma mobile visualized by surface protein localization
    Akiko Kusumoto
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Microbiology 150:4001-8. 2004
    ..This analysis shows that M. mobile surprisingly expresses multiple versions of its major surface antigen at once but differentiates its surface by differential localization of the various paralogues...
  15. pmc Involvement of P1 adhesin in gliding motility of Mycoplasma pneumoniae as revealed by the inhibitory effects of antibody under optimized gliding conditions
    Shintaro Seto
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 187:1875-7. 2005
    ....
  16. pmc Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging
    Isil Tulum
    Department of Biology, Graduate School of Science, Osaka City University, Osaka, Japan
    J Bacteriol 196:1815-24. 2014
    ..The roles of P42 and the F(1)-ATPase subunit homolog are discussed as part of our proposed gliding mechanism...
  17. pmc Triskelion structure of the Gli521 protein, involved in the gliding mechanism of Mycoplasma mobile
    Takahiro Nonaka
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 192:636-42. 2010
    ..Partial digestion of Gli521 showed that the molecule could be divided into three domains, which we assigned to the oval, rod, and hook of the molecular image. The Gli521 molecule's role in the gliding mechanism is discussed...
  18. pmc Morphology of isolated Gli349, a leg protein responsible for Mycoplasma mobile gliding via glass binding, revealed by rotary shadowing electron microscopy
    Jun Adan-Kubo
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 188:2821-8. 2006
    ..The amino acid sequence was assigned to the molecular image, and the topology of the molecule in the gliding machinery is discussed...
  19. pmc Gliding ghosts of Mycoplasma mobile
    Atsuko Uenoyama
    Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Proc Natl Acad Sci U S A 102:12754-8. 2005
    ..The reactivation activity and inhibition by various nucleotides and ATP analogs, as well as their kinetic parameters, showed that the machinery is driven by the hydrolysis of ATP to ADP plus phosphate, caused by an unknown ATPase...
  20. pmc Identification of a 521-kilodalton protein (Gli521) involved in force generation or force transmission for Mycoplasma mobile gliding
    Shintaro Seto
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    J Bacteriol 187:3502-10. 2005
    ....
  21. pmc Attachment organelle formation represented by localization of cytadherence proteins and formation of the electron-dense core in wild-type and mutant strains of Mycoplasma pneumoniae
    Shintaro Seto
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 185:1082-91. 2003
    ..These results suggest that there are three distinct subcellular protein localization sites in the attachment organelle, which were represented by HMW1-HMW3, P1-P90-P40, and P30-P65...
  22. pmc Role of binding in Mycoplasma mobile and Mycoplasma pneumoniae gliding analyzed through inhibition by synthesized sialylated compounds
    Taishi Kasai
    Department of Biology, Graduate School of Science, Osaka City University, Osaka, Japan
    J Bacteriol 195:429-35. 2013
    ..iii) Mycoplasma legs may generate a drag force after a stroke, because the gliding speed decreased and pivoting motion occurred more frequently when the number of working legs was reduced by the addition of free sialylated compounds...
  23. pmc Molecular structure of isolated MvspI, a variable surface protein of the fish pathogen Mycoplasma mobile
    Jun Adan-Kubo
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Bacteriol 194:3050-7. 2012
    ..The amino acid sequence of MvspI was mapped onto a molecular image obtained by electron microscopy. The present study is the first to elucidate the molecular shape of a variable surface protein of mycoplasma...
  24. ncbi request reprint [Gliding motility of mycoplasma--a mechanism cannot be explained by today's biology]
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585
    Nihon Saikingaku Zasshi 57:581-95. 2002
  25. pmc Gliding motility of Mycoplasma mobile can occur by repeated binding to N-acetylneuraminyllactose (sialyllactose) fixed on solid surfaces
    Ryoichiro Nagai
    PRESTO, JST, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 188:6469-75. 2006
    ....
  26. pmc Whole surface image of Mycoplasma mobile, suggested by protein identification and immunofluorescence microscopy
    Heng Ning Wu
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Bacteriol 194:5848-55. 2012
    ..mobile account for more than 12% of all surface proteins and that Mvsps, surface variants that were localized at both parts other than the neck, account for 49% of all surface proteins...
  27. pmc Mycoplasma mobile cells elongated by detergent and their pivoting movements in gliding
    Daisuke Nakane
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka, Japan
    J Bacteriol 194:122-30. 2012
    ..The torque required to achieve the actual pivoting increased with the cell length without saturation, reaching 54.7 pN nm at 4.3 μm in cell length...
  28. ncbi request reprint [Molecular mechanism of Mycoplasma gliding; a unique biomotility]
    Makoto Miyata
    Department of Biology, Graduate School of Science, Osaka City University
    Nihon Saikingaku Zasshi 62:347-61. 2007
  29. pmc Energetics of gliding motility in Mycoplasma mobile
    Jacob D Jaffe
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
    J Bacteriol 186:4254-61. 2004
    ..Sodium orthovanadate had a slight but significant inhibitory effect on gliding. Taken together, these results suggest that the motor system of M. mobile is likely an ATPase or is directly coupled to an ATPase...
  30. pmc Identification of a 123-kilodalton protein (Gli123) involved in machinery for gliding motility of Mycoplasma mobile
    Atsuko Uenoyama
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 187:5578-84. 2005
    ..Localization of the gliding proteins, Gli349 and Gli521, was disturbed in the m12 mutant, suggesting that Gli123 is essential for the positioning of gliding proteins in the cell neck...
  31. pmc Mapping of replication initiation site in Mycoplasma capricolum genome by two-dimensional gel-electrophoretic analysis
    M Miyata
    Department of Biology, Faculty of Science, Osaka City University, Japan
    Nucleic Acids Res 21:4816-23. 1993
    ..The forks developed in a 1569-bp region that consisted of the dnaA structural gene and its downstream non-coding region, and then they proceeded bidirectionally...
  32. doi request reprint "Mycoplasmal antigen modulation," a novel surface variation suggested for a lipoprotein specifically localized on Mycoplasma mobile
    Heng Ning Wu
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Curr Microbiol 64:433-40. 2012
    ..The decrease in MvspI caused by the antibody binding may suggest a novel type of surface variation, designated here as "mycoplasmal antigen modulation."..
  33. pmc Identification of a novel nucleoside triphosphatase from Mycoplasma mobile: a prime candidate motor for gliding motility
    Naoto Ohtani
    Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Biochem J 403:71-7. 2007
    ..These results support the hypothesis that the P42 protein is the key ATPase in the gliding motility of M. mobile...
  34. ncbi request reprint Characterization of dnaA gene expression in Mycoplasma capricolum
    S Seto
    Department of Biology, Faculty of Science, Osaka City University, Japan
    FEMS Microbiol Lett 150:239-47. 1997
    ..These observations showed that the expression pattern of dnaA gene in M. capricolum is different from those of walled bacteria in some aspects...
  35. pmc Cytoskeletal asymmetrical dumbbell structure of a gliding mycoplasma, Mycoplasma gallisepticum, revealed by negative-staining electron microscopy
    Daisuke Nakane
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    J Bacteriol 191:3256-64. 2009
    ..The cytoskeletal structures of M. gallisepticum were compared with those of M. pneumoniae in detail, and the possible protein components of these structures were considered...
  36. pmc Unitary step of gliding machinery in Mycoplasma mobile
    Yoshiaki Kinosita
    Department of Physics, Faculty of Science, Gakushuin University, Toshima ku, Tokyo 171 8588, Japan
    Proc Natl Acad Sci U S A 111:8601-6. 2014
    ....
  37. ncbi request reprint Electron microscopic studies of three gliding Mycoplasmas, Mycoplasma mobile, M. pneumoniae, and M. gallisepticum, by using the freeze-substitution technique
    Takashi Shimizu
    Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
    Curr Microbiol 44:431-4. 2002
    ..The cells of M. gallisepticum presented irregular dense granules, in contrast to regular particles, which can be observed in the images of chemically fixed thin sections, in the rear portion of the cells...
  38. ncbi request reprint The gyrB gene lies opposite from the replication origin on the circular chromosome of Mycoplasma capricolum
    K Sano
    Department of Biology, Faculty of Science, Osaka City University, Japan
    Gene 151:181-3. 1994
    ..2-kb region which contains the gyrB gene, encoding the beta-subunit of gyrase from Mycoplasma capricolum. We localized gyrB opposite from the chromosomal replication origin (ori) of M. capricolum by genomic Southern hybridization...
  39. pmc Use of fluorescent-protein tagging to determine the subcellular localization of mycoplasma pneumoniae proteins encoded by the cytadherence regulatory locus
    Tsuyoshi Kenri
    Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Bacteriol 186:6944-55. 2004
    ..The fluorescent-protein fusion technique may serve as a powerful tool for identifying components of cytoskeleton-like structures and the attachment organelle. It can also be used to analyze their assembly...
  40. pmc A microrotary motor powered by bacteria
    Yuichi Hiratsuka
    Gene Function Research Center, Advanced Semiconductor Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Proc Natl Acad Sci U S A 103:13618-23. 2006
    ..This motor is fueled by glucose and inherits some of the properties normally attributed to living systems...
  41. ncbi request reprint Living microtransporter by uni-directional gliding of Mycoplasma along microtracks
    Yuichi Hiratsuka
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Biochem Biophys Res Commun 331:318-24. 2005
    ..Furthermore, cells loaded with streptavidin beads following biotinylation of surface proteins moved at normal speeds. These bacteria could be useful as living microtransporters, carrying cargo around within micrometer-scale spaces...