Taroh Kinoshita

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. ncbi Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress
    Taroh Kinoshita
    WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    J Biochem 144:287-94. 2008
  2. ncbi Different roles of glycosylphosphatidylinositol in various hematopoietic cells as revealed by a mouse model of paroxysmal nocturnal hemoglobinuria
    Y Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Blood 94:2963-70. 1999
  3. ncbi Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis
    T Kinoshita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
    Curr Opin Chem Biol 4:632-8. 2000
  4. ncbi Designing sleeping sickness control
    Taroh Kinoshita
    Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, and Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    ACS Chem Biol 3:601-3. 2008
  5. ncbi Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I
    Hisashi Ashida
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 16:1439-48. 2005
  6. ncbi DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3
    Hisashi Ashida
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    J Biol Chem 281:896-904. 2006
  7. ncbi PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins
    Yuko Tashima
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 17:1410-20. 2006
  8. ncbi GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol
    Nobue Shishioh
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 280:9728-34. 2005
  9. ncbi PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol
    Ji Young Kang
    Department of Immunoregulation, Research Institute for Microbial Diseases, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 280:9489-97. 2005
  10. ncbi Peroxisome dependency of alkyl-containing GPI-anchor biosynthesis in the endoplasmic reticulum
    Noriyuki Kanzawa
    Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 106:17711-6. 2009

Collaborators

Detail Information

Publications109 found, 100 shown here

  1. ncbi Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress
    Taroh Kinoshita
    WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    J Biochem 144:287-94. 2008
    ..It is thought that association with membrane rafts is important for GPI-APs in signal transduction and other functions. Here, we review recent progress in studies on biosynthesis, remodelling and functions of mammalian GPI-APs...
  2. ncbi Different roles of glycosylphosphatidylinositol in various hematopoietic cells as revealed by a mouse model of paroxysmal nocturnal hemoglobinuria
    Y Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Blood 94:2963-70. 1999
    ....
  3. ncbi Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis
    T Kinoshita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
    Curr Opin Chem Biol 4:632-8. 2000
    ..These studies have revealed the common and also different characteristics of glycosylphosphatidyl-inositol biosynthesis enzymes in different organisms, leading to the development of species-specific inhibitors of the pathway...
  4. ncbi Designing sleeping sickness control
    Taroh Kinoshita
    Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, and Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    ACS Chem Biol 3:601-3. 2008
    ..An article in this issue reports the first chemically synthesized inhibitor of the third mannosyltransferase from the GPI pathway, stimulating further investigation toward practical and useful compounds...
  5. ncbi Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I
    Hisashi Ashida
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 16:1439-48. 2005
    ..This is the first report of an essential subcomponent of glycosyltransferases using dolichol-phosphate-monosaccharide...
  6. ncbi DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3
    Hisashi Ashida
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    J Biol Chem 281:896-904. 2006
    ..Free DPM1 was strongly associated with the C terminus of Hsc70-interacting protein (CHIP), a chaperone-dependent E3 ubiquitin ligase, suggesting that DPM1 is ubiquitinated, at least in part, by CHIP...
  7. ncbi PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins
    Yuko Tashima
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 17:1410-20. 2006
    ..These results demonstrate that PGAP2 is involved in the processing of GPI-APs required for their stable expression at the cell surface...
  8. ncbi GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol
    Nobue Shishioh
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 280:9728-34. 2005
    ..Finally, we propose a mechanism for the regulation of GPI biosynthesis through competition between the two independent enzymes, PIG-O and hGPI7, for the common stabilizer, PIG-F...
  9. ncbi PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol
    Ji Young Kang
    Department of Immunoregulation, Research Institute for Microbial Diseases, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 280:9489-97. 2005
    ..PIG-V has two functionally important conserved regions facing the ER lumen. Taken together, we suggest that PIG-V is the second mannosyltransferase in GPI anchor biosynthesis...
  10. ncbi Peroxisome dependency of alkyl-containing GPI-anchor biosynthesis in the endoplasmic reticulum
    Noriyuki Kanzawa
    Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 106:17711-6. 2009
    ..These results highlight cooperation of three organelles, the ER, the Golgi, and the peroxisome, in the generation of the lipid portion of GPI-APs...
  11. ncbi PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 14:4285-95. 2003
    ..The inositol acylation is not essential for the subsequent mannosylation, indicating that glucosaminyl phosphatidylinositol can flip from the cytoplasmic side to the luminal side of the endoplasmic reticulum...
  12. ncbi PGAP1 knock-out mice show otocephaly and male infertility
    Yasutaka Ueda
    Department of Immunoregulation, Osaka University, Osaka, Japan
    J Biol Chem 282:30373-80. 2007
    ..Therefore, an extra acyl chain in GPI anchors caused severe deleterious effects to development and sperm function...
  13. ncbi Removal or maintenance of inositol-linked acyl chain in glycosylphosphatidylinositol is critical in trypanosome life cycle
    Yeonchul Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    J Biol Chem 281:11595-602. 2006
    ..Therefore, the GPI inositol deacylase activity must be tightly regulated in trypanosome life cycle...
  14. ncbi Enhanced responses of glycosylphosphatidylinositol anchor-deficient T lymphocytes
    Wouter L W Hazenbos
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    J Immunol 173:3810-5. 2004
    ..These results indicate that coligation with GPI-anchored proteins counteracts the response to TCR stimulation by ConA or alloantigen but not protein Ag...
  15. ncbi Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins
    Yeongjin Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    Mol Biol Cell 14:1780-9. 2003
    ..Taken together, PIG-U and the yeast orthologue Cdc91p are the fifth component of GPI transamidase that may be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI...
  16. ncbi TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei
    Yeonchul Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    FEBS Lett 580:603-6. 2006
    ....
  17. ncbi The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 16:5236-46. 2005
    ..These two proteins are translated from one mRNA by leaky scanning of the PreY initiation site...
  18. ncbi Defective lipid remodeling of GPI anchors in peroxisomal disorders, Zellweger syndrome, and rhizomelic chondrodysplasia punctata
    Noriyuki Kanzawa
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    J Lipid Res 53:653-63. 2012
    ..Absence of the 1-alkyl-2-acyl form of GPI-anchored proteins might account for some of the complex phenotypes of these two major peroxisomal disorders...
  19. ncbi Deregulated expression of HMGA2 is implicated in clonal expansion of PIGA deficient cells in paroxysmal nocturnal haemoglobinuria
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases Department of Immunoglycobiology, WPI Immunology Frontier Research Centre, Osaka, Japan
    Br J Haematol 156:383-7. 2012
    ..Over-expression of truncated HMGA2 in mouse bone marrow cells caused expansion in recipient mice. These results support the idea that deregulated expression of HMGA2 causes expansion of PNH cells...
  20. ncbi Identification of a second catalytically active trans-sialidase in Trypanosoma brucei
    Fumiki Nakatani
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Biochem Biophys Res Commun 415:421-5. 2011
    ..We suggest that TbSA C2 plays a role in the sialic acid modification of the trypanosome cell surface...
  21. ncbi Mechanism for release of alkaline phosphatase caused by glycosylphosphatidylinositol deficiency in patients with hyperphosphatasia mental retardation syndrome
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, and Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Osaka, Japan 565 0871, Japan
    J Biol Chem 287:6318-25. 2012
    ..These results explain the molecular mechanism of hyperphosphatasia in HPMR...
  22. ncbi Structural remodeling, trafficking and functions of glycosylphosphatidylinositol-anchored proteins
    Yusuke Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Prog Lipid Res 50:411-24. 2011
    ....
  23. ncbi Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane
    Satoshi Takida
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Biochem J 409:555-62. 2008
    ..Trafficking of non-GPI-linked cargo proteins was barely affected by p23 knockdown. This is the first to demonstrate direct evidence for the transport of mammalian GPI-anchored proteins being mediated by p24 proteins...
  24. ncbi Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p
    Satoshi Tanaka
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 279:14256-63. 2004
    ..The mutant cells showed a clear delay in the maturation of GPI-APs in the Golgi and accumulation of GPI-APs in the ER. Thus, the GPI inositol deacylation is important for efficient transport of GPI-APs from the ER to the Golgi...
  25. ncbi Enhanced response of T lymphocytes from Pgap3 knockout mouse: Insight into roles of fatty acid remodeling of GPI anchored proteins
    Hidekazu Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Japan
    Biochem Biophys Res Commun 417:1235-41. 2012
    ..These results suggest that GPI-APs localized in lipid rafts may modulate signaling through the TCR...
  26. ncbi Fatty acid remodeling of GPI-anchored proteins is required for their raft association
    Yusuke Maeda
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 18:1497-506. 2007
    ..We also show that the remodeling requires the preceding PGAP1-mediated deacylation from inositol of GPI-APs in the endoplasmic reticulum...
  27. ncbi GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi
    Morihisa Fujita
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Cell 139:352-65. 2009
    ..Our data demonstrate that GPI glycan acts as an ER-exit signal and suggest that glycan remodeling mediated by PGAP5 regulates GPI-AP transport in the early secretory pathway...
  28. ncbi Dolichol-phosphate mannose synthase: structure, function and regulation
    Yusuke Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Biochim Biophys Acta 1780:861-8. 2008
    ..In this review, we focus on the biosynthesis of dolichol-phosphate-mannose, and particularly on the mammalian enzyme complex involved in the reaction...
  29. ncbi Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI
    Morihisa Fujita
    Research Institute for Microbial Diseases and WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565 0871, Japan
    J Cell Biol 194:61-75. 2011
    ..Our results indicate that p24 complexes act as cargo receptors for correctly remodeled GPI-APs to be sorted into COPII vesicles...
  30. ncbi Controlled expression of branch-forming mannosyltransferase is critical for mycobacterial lipoarabinomannan biosynthesis
    Chubert B C Sena
    Research Institute for Microbial Diseases and WPI Immunology Frontier Research Center, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 285:13326-36. 2010
    ..These data suggest that the coordinated expressions of branching and elongating mannosyltransferases are critical for mannan backbone elongation...
  31. ncbi Inositol lipid metabolism in mycobacteria: biosynthesis and regulatory mechanisms
    Yasu S Morita
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Biochim Biophys Acta 1810:630-41. 2011
    ..The biosynthesis of these lipids is believed to be prime drug targets, and has been clarified in detail over the past several years...
  32. ncbi GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus
    Yusuke Maeda
    Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Nat Cell Biol 10:1135-45. 2008
    ..After reconstitution in planar lipid bilayers, GPHR exhibited a voltage-dependent anion-channel activity that may function in counterion conductance. Thus, GPHR modulates Golgi functions through regulation of acidification...
  33. ncbi GPI-anchor remodeling: potential functions of GPI-anchors in intracellular trafficking and membrane dynamics
    Morihisa Fujita
    Research Institute for Microbial Diseases and WPI Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochim Biophys Acta 1821:1050-8. 2012
    ..Here, we describe the structural remodeling and function of GPI-anchors, and discuss how GPI-anchors regulate protein sorting, trafficking, and dynamics. This article is part of a Special Issue entitled Lipids and Vesicular Transport...
  34. ncbi Inefficient response of T lymphocytes to glycosylphosphatidylinositol anchor-negative cells: implications for paroxysmal nocturnal hemoglobinuria
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Blood 100:4116-22. 2002
    ..These results provide the first experimental evidence for the hypothesis that GPI(-) cells escape from immunologic attack...
  35. ncbi The acidic environment of the Golgi is critical for glycosylation and transport
    Yusuke Maeda
    Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Methods Enzymol 480:495-510. 2010
    ..In this chapter, we have introduced how we established mutant cells with impaired Golgi acidification and methods for measuring Golgi pH...
  36. ncbi CHO glycosylation mutants: GPI anchor
    Yusuke Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    Methods Enzymol 416:182-205. 2006
    ..In this chapter, we overview GPI-AP biosynthesis, establishment and characterization of GPI-AP-defective mutant cell lines, expression cloning using those mutant cells, and characteristics of GPI-AP-defective mutant cell lines...
  37. ncbi GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits
    Kisaburo Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 100:10682-7. 2003
    ....
  38. ncbi PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria
    Yasu S Morita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 281:25143-55. 2006
    ..Taken together, PimE represents the first PPM-dependent mannosyl-transferase shown to be involved in PIM biosynthesis, where it mediates the fifth mannose transfer...
  39. ncbi Molecular genetics of paroxysmal nocturnal hemoglobinuria
    Norimitsu Inoue
    Department of Molecular Genetics, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka University, Suita, Osaka, Japan
    Int J Hematol 77:107-12. 2003
    ..The next 10 years should witness the discovery of the molecular mechanisms of immunologic selection and the identification of abnormalities involved in the further clonal expansion of PNH cells...
  40. ncbi The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    EMBO J 17:877-85. 1998
    ..These results suggest that GPI-GnT recognizes the fatty acyl chains of PI. The unusually complex organization of GPI-GnT may be relevant to selective usage of PI and/or regulation...
  41. ncbi Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    EMBO J 19:4402-11. 2000
    ..These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI...
  42. ncbi Structural remodeling of GPI anchors during biosynthesis and after attachment to proteins
    Morihisa Fujita
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    FEBS Lett 584:1670-7. 2010
    ..Here, we describe the structural remodeling of GPI anchors and genes required for the processes in mammals, yeast, and trypanosomes...
  43. ncbi Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
    Gen Kondoh
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, 2 2 Yamadaoka, Osaka 565 0871, Japan
    Nat Med 11:160-6. 2005
    ..Moreover, peptidase-inactivated E414D mutant ACE and also PI-PLC rescued the egg-binding deficiency of Ace knockout sperms, implying that ACE plays a crucial role in fertilization through this activity...
  44. ncbi Surface sialic acids taken from the host allow trypanosome survival in tsetse fly vectors
    Kisaburo Nagamune
    Dept. of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    J Exp Med 199:1445-50. 2004
    ..Thus, surface sialic acids appear to protect the parasites from the digestive and trypanocidal environments in the midgut of Tsetse flies...
  45. ncbi GPI-anchor deficiency in myeloid cells causes impaired FcgammaR effector functions
    Wouter L W Hazenbos
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Blood 104:2825-31. 2004
    ..This study provides the first genetic evidence for an essential role of GPI-anchored proteins in physiologic FcgammaR effector functions in vitro and in vivo...
  46. ncbi Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge
    Kazuhito Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    J Biol Chem 278:13959-67. 2003
    ....
  47. ncbi PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan
    J Biol Chem 271:26868-75. 1996
    ..Taken together with the cytoplasmic orientation of GlcNAc-PI, these results indicated that PIG-A and PIG-H are subunits of the GPI GlcNAc transferase that transfers GlcNAc to PI on the cytoplasmic side of the ER...
  48. ncbi Critical roles for lipomannan and lipoarabinomannan in cell wall integrity of mycobacteria and pathogenesis of tuberculosis
    Takeshi Fukuda
    Research Institute for Microbial Diseases, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    MBio 4:e00472-12. 2013
    ..These findings will now allow us to seek the possibility that the LM/LAM biosynthetic pathway is a chemotherapeutic target...
  49. ncbi Integrin-linked kinase associated with integrin activation
    Shigenori Honda
    National Cardiovascular Center Research Institute, Osaka, Japan
    Blood 113:5304-13. 2009
    ..Our present data suggest that ILK contributes to inside-out integrin activation...
  50. ncbi Requirement of N-glycan on GPI-anchored proteins for efficient binding of aerolysin but not Clostridium septicum alpha-toxin
    Yeongjin Hong
    Departments of Immunoregulation and Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    EMBO J 21:5047-56. 2002
    ....
  51. ncbi [Biosynthesis of GPI-anchored proteins]
    Satoshi Tanaka
    Tanpakushitsu Kakusan Koso 48:990-6. 2003
  52. ncbi Stress-induced synthesis of phosphatidylinositol 3-phosphate in mycobacteria
    Yasu S Morita
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, Parkville, Victoria 3010, Australia
    J Biol Chem 285:16643-50. 2010
    ....
  53. ncbi [GPI biosynthetic pathway as a target for antiprotozoan drugs]
    Kisaburo Nagamune
    Tanpakushitsu Kakusan Koso 47:30-6. 2002
  54. ncbi Functional outcomes after extended surgery for gastric cancer
    Y Kurokawa
    Department of Surgery, Osaka National Hospital, Osaka, Japan
    Br J Surg 98:239-45. 2011
    ..Extended gastrectomy with para-aortic nodal dissection (PAND) or thorough dissection of mediastinal nodes using a left thoracoabdominal (LTA) approach is an alternative to D2 lymphadenectomy, with variable postoperative results...
  55. ncbi PIG-C, one of the three human genes involved in the first step of glycosylphosphatidylinositol biosynthesis is a homologue of Saccharomyces cerevisiae GPI2
    N Inoue
    Department of Immunoregulation, Osaka University, Japan
    Biochem Biophys Res Commun 226:193-9. 1996
    ..Since there are several human EST sequences that have homology to GPI1, our results suggest that four genes are involved in the first step of GPI anchor synthesis in mammalian cells...
  56. ncbi Deficiency of the GPI anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria
    J Takeda
    Department of Immunoregulation, Osaka University, Japan
    Cell 73:703-11. 1993
    ..We localized PIG-A to the X chromosome, which accounts for expression of the recessive phenotype of the somatic mutation and the fact that the same one of the multiple biosynthetic steps is affected in all patients so far characterized...
  57. ncbi Structure of the zinc endoprotease from Streptomyces caespitosus
    G Kurisu
    Department of Applied Chemistry, Faculty of Engineering, Osaka University
    J Biochem 121:304-8. 1997
    ..Thus, ScNP represents a novel subfamily of metzincins with a HEXXHXXGXXD zinc-binding sequence. A plausible substrate recognition pocket to which aromatic residues bind is located near the catalytic zinc ion...
  58. ncbi Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria
    K Kawagoe
    Department of Immunoregulation, Osaka University, Japan
    Blood 87:3600-6. 1996
    ....
  59. ncbi Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol
    Y Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 275:20911-9. 2000
    ..Therefore, mammalian cells have redundant activities in transferring phosphoethanolamine to the third mannose, both of which require PIG-F...
  60. ncbi Kola acuminata proanthocyanidins: a class of anti-trypanosomal compounds effective against Trypanosoma brucei
    B K Kubata
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6 2 4 Furuedai, Suita, Osaka 565 0874, Japan
    Int J Parasitol 35:91-103. 2005
    ..This new anti-trypanosomal compound should be further studied to determine its efficacy and suitability as an anti-trypanosomal drug and may be used as a tool to define novel specific drug targets in BF trypanosomes...
  61. ncbi Safety and efficacy of the terminal complement inhibitor eculizumab in Japanese patients with paroxysmal nocturnal hemoglobinuria: the AEGIS clinical trial
    Yuzuru Kanakura
    Department of Hematology and Oncology, Osaka University Hospital, Suita, Japan
    Int J Hematol 93:36-46. 2011
    ..001). The AEGIS results demonstrate that eculizumab is effective, safe and well tolerated in Japanese patients with PNH...
  62. ncbi Prostacyclin-deficient mice develop ischemic renal disorders, including nephrosclerosis and renal infarction
    Chieko Yokoyama
    Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka University, Suita, Osaka, Japan
    Circulation 106:2397-403. 2002
    ..To study the role of PGI2 in the vascular system in vivo, PGI2-deficient (PGID) mice were established by genetic disruption of the PGI2 synthase gene...
  63. ncbi Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene
    Kosuke Yusa
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 429:896-9. 2004
    ..Our results indicate that phenotype-based genetic screening with Blm(tet) is very efficient and raises possibilities for identifying gene functions in ES cells...
  64. ncbi Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus
    K Kawagoe
    Department of Immunoregulation, Osaka University, Japan
    Genomics 23:566-74. 1994
    ..Database analysis demonstrated that a yeast gene, SPT14, is homologous to Pig-a and PIGA and that these genes are members of a glycosyltransferase gene family...
  65. ncbi Structure and chromosomal localization of the GPI-anchor synthesis gene PIGF and its pseudogene psi PIGF
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    Genomics 29:804-7. 1995
    ..We also identified the processed pseudogene of PIGF (psi PIGF) and mapped it to 5q35...
  66. ncbi Pig-n, a mammalian homologue of yeast Mcd4p, is involved in transferring phosphoethanolamine to the first mannose of the glycosylphosphatidylinositol
    Y Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biol Chem 274:35099-106. 1999
    ..Biosynthesis of GPI in the Pig-n knockout cells was not affected by YW3548/BE49385A, and yeast overexpressing MCD4 was highly resistant to YW3548/BE49385A, suggesting that Pig-n and Mcd4p are targets of this drug...
  67. ncbi Developmental abnormalities of glycosylphosphatidylinositol-anchor-deficient embryos revealed by Cre/loxP system
    M Nozaki
    Department of Molecular Embryology, Research Institute for Microbial Diseases, Osaka University, Japan
    Lab Invest 79:293-9. 1999
    ..These data further highlight the importance of GPI-anchored proteins during mouse embryonic development...
  68. ncbi Implications of lipid moiety in oligomerization and immunoreactivities of GPI-anchored proteins
    Jihyoun Seong
    Department of Immunoregulation, Research Institute for Microbial Diseases, and Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Osaka University, Osaka 565 0871, Japan
    J Lipid Res 54:1077-91. 2013
    ..Thus, this study clarifies the significance of GPI fatty acid remodeling in oligomerization of GPI-APs and provides useful information for technical studies of these cell components...
  69. ncbi Long-term support of hematopoiesis by a single stem cell clone in patients with paroxysmal nocturnal hemoglobinuria
    Jun ichi Nishimura Ji
    Department of Immunoregulation Research Institute for Microbial Diseases and the Research Foundation for Microbial Diseases, Osaka University, Japan
    Blood 99:2748-51. 2002
    ..Two patients whose affected PMNs decreased because of a decline of the predominant PNH clone and who have been followed up for 24 and 31 years now have an aplastic condition, suggesting that aplasia is a terminal feature of PNH...
  70. ncbi Acquisition of complement resistance through incorporation of CD55/decay-accelerating factor into viral particles bearing baculovirus GP64
    Yuuki Kaname
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Virol 84:3210-9. 2010
    ..These results suggest that the incorporation of human DAF into viral particles by interacting with baculovirus GP64 is involved in the acquisition of resistance to serum inactivation...
  71. ncbi Relationship between aplastic anemia and paroxysmal nocturnal hemoglobinuria
    Taroh Kinoshita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Int J Hematol 75:117-22. 2002
    ..Survival of PNH clones in pathologic bone marrow may account for limited expansion of PNH clones; however, additional genetic change(s) that confers cells with growth phenotype may be required for the full development of PNH...
  72. ncbi Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system
    Vincent W Keng
    Center for Advanced Science and Innovation, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Methods 2:763-9. 2005
    ..All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region...
  73. ncbi [Complement system in host defense]
    Kazuhito Ohishi
    Department of Immunoregulation, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University
    Nippon Rinsho 63:263-8. 2005
  74. ncbi GPI1 stabilizes an enzyme essential in the first step of glycosylphosphatidylinositol biosynthesis
    Y Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    J Biol Chem 274:18582-8. 1999
    ..A lack of GPI1 also caused partial decreases of PIG-C and PIG-H. Therefore, GPI1 stabilizes the enzyme by tying up PIG-C with a complex of PIG-A and PIG-H...
  75. ncbi Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase
    N Nakamura
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565, Japan
    J Biol Chem 272:15834-40. 1997
    ..This orientation of PIG-L protein is consistent with the notion that the second step of GPI anchor biosynthesis occurs on the cytoplasmic side of the endoplasmic reticulum...
  76. ncbi Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development
    M Tarutani
    Department of Dermatology, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565, Japan
    Proc Natl Acad Sci U S A 94:7400-5. 1997
    ..Mutant mice died within a few days after birth, suggesting that Pig-a function is essential for proper skin differentiation and maintenance...
  77. ncbi Structures and chromosomal localizations of the glycosylphosphatidylinositol synthesis gene PIGC and its pseudogene PIGCP1
    Y Hong
    Department of Immunoregulation, Osaka University, Japan
    Genomics 44:347-9. 1997
    ..PIGC is an intronless gene. We found an intronless pseudogene of PIGC, PIGCP1, and mapped it to chromosome 11p12-p13. The presence of a processed pseudogene is a common feature of PIGA, PIGF, and PIGC...
  78. ncbi The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis
    T Miyata
    Department of Immunoregulation, Osaka University, Japan
    Science 259:1318-20. 1993
    ..PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis...
  79. ncbi Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis
    N Kishi
    Division of Neuroanatomy, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, 565 0871, Osaka, Japan
    Int J Dev Neurosci 19:21-35. 2001
    ..Collectively, our results suggest that these mouse deltex homologs are involved in vertebrate Notch signaling and regulation of neurogenesis...
  80. ncbi Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem J 339:185-92. 1999
    ..The disruption of the gene caused lethality in S. cerevisiae. These results indicate that GlcNAc-PI de-N-acetylase is conserved between mammals and yeasts and that the de-N-acetylation step is also indispensable in yeasts...
  81. ncbi Expression cloning of genes for GPI-anchor biosynthesis
    T Kinoshita
    Department of Immunoregulation, Osaka University, Japan
    Braz J Med Biol Res 27:127-32. 1994
    ..PIG-B cDNA restored the surface expression of Thy-1 on SIA-b cells and also synthesis of mature type GPI-anchor precursors in these cells.(ABSTRACT TRUNCATED AT 250 WORDS)..
  82. ncbi PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor
    M Takahashi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    EMBO J 15:4254-61. 1996
    ..This autosomal location would explain why PIG-B is not involved in the defective GPI anchor synthesis in paroxysmal nocturnal hemoglobinuria, which is always caused by a somatic mutation of the X-linked PIG-A gene...
  83. ncbi PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER
    Y Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    EMBO J 20:250-61. 2001
    ....
  84. ncbi Cloning and characterization of the murine GPI anchor synthesis gene Pigf, a homologue of the human PIGF gene
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, 565, Japan
    Genomics 34:340-6. 1996
    ..The results also extend a region in murine distal chromosome 17 that is syntenic to human chromosome 2p16-p22...
  85. ncbi DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate
    Y Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    EMBO J 17:4920-9. 1998
    ..Mammalian DPM1 is catalytic because a fusion protein of DPM1 that was stably expressed in the ER synthesized DPM without DPM2. Therefore, biosynthesis of DPM in mammalian cells is regulated by DPM2...
  86. ncbi A homologue of Saccharomyces cerevisiae Dpm1p is not sufficient for synthesis of dolichol-phosphate-mannose in mammalian cells
    S Tomita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    J Biol Chem 273:9249-54. 1998
    ..Therefore, in contrast to yeast, mammalian cells require hDPM1/mDPM1 protein and a product of another gene that is defective in Lec15 mutant cells for synthesis of Dol-P-Man...
  87. ncbi Human dolichol-phosphate-mannose synthase consists of three subunits, DPM1, DPM2 and DPM3
    Y Maeda
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    EMBO J 19:2475-82. 2000
    ..Schizosaccharomyces pombe has proteins that resemble three human subunits; S.pombe DPM3 restored biosynthesis of DPM in Lec15 cells, indicating its orthologous relationship to human DPM3...
  88. ncbi PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    EMBO J 20:4088-98. 2001
    ..Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively...
  89. ncbi Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei
    K Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:10336-41. 2000
    ..Infectivity to tsetse flies is partially impaired, particularly in the early stage. Therefore, parasitespecific inhibition of GPI biosynthesis should be an effective chemotherapy target against African trypanosomiasis...
  90. ncbi Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 11:1523-33. 2000
    ..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment...
  91. ncbi New mutant Chinese hamster ovary cell representing an unknown gene for attachment of glycosylphosphatidylinositol to proteins
    Yeongjin Hong
    Genomic Research Center for Enteropathogenic Bacteria and Department of Microbiology, Chonnam National University Medical School, Gwangju, Republic of Korea
    Biochem Biophys Res Commun 335:1060-9. 2005
    ..Microsomes from CHOPA41.3 cells had normal GPI transamidase activity. Taken together, there is an unknown gene required for efficient attachment of GPI to proteins...
  92. ncbi Erythrocyte surface glycosylphosphatidyl inositol anchored receptor for the malaria parasite
    Thanaporn Rungruang
    Department of Molecular Parasitology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
    Mol Biochem Parasitol 140:13-21. 2005
    ..We conclude that the major erythrocyte receptor for PyRhopH complex is a protein attached to the erythrocyte surface via GPI-anchor and that GPI-deficient erythrocytes are resistant to P. yoelii invasion...
  93. ncbi [New strategy in development of antibiotics: GPI biosynthesis as a target]
    Taroh Kinoshita
    Jpn J Antibiot 59:125-32. 2006
  94. ncbi Molecular basis of clonal expansion of hematopoiesis in 2 patients with paroxysmal nocturnal hemoglobinuria (PNH)
    Norimitsu Inoue
    Department of Molecular Genetics, Osaka Medical Center for Cancer, Japan
    Blood 108:4232-6. 2006
    ..These observations suggest that aberrant HMGA2 expression, in concert with mutant PIGA, accounts for clonal hematopoiesis in these 2 patients and suggest the concept of PNH as a benign tumor of the bone marrow...
  95. ncbi Isolation of new CHO cell mutants defective in CMP-sialic acid biosynthesis and transport
    Dong-Jun Shin
    Genomic Research Center for Enteropathogenic Bacteria and Department of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Korea
    Mol Cells 22:343-52. 2006
    ..These results indicate that the novel missense mutations isolated in this study affect CST activity...
  96. ncbi Decreased susceptibility of leukemic cells with PIG-A mutation to natural killer cells in vitro
    Shoichi Nagakura
    Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan
    Blood 100:1031-7. 2002
    ..Our experiments suggest that PIG-A mutations confer a relative survival advantage to a PNH clone, contributing to selective expansion of these cells in the setting of marrow injury by cytotoxic lymphocytes...
  97. ncbi Changes in molecular species profiles of glycosylphosphatidylinositol anchor precursors in early stages of biosynthesis
    Toshiaki Houjou
    Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    J Lipid Res 48:1599-606. 2007
    ..Therefore, a specific feature of the PI moieties of mature GPI-APs, domination of alkyl (or alkenyl)/acyl type species over diacyl types, is established at the stage of GlcN-acyl-PI...
  98. ncbi The glycan core of GPI-anchored proteins modulates aerolysin binding but is not sufficient: the polypeptide moiety is required for the toxin-receptor interaction
    Laurence Abrami
    Department of Genetics and Microbiology, University of Geneva, 1 rue Michel Servet, 1211 4, Geneva, Switzerland
    FEBS Lett 512:249-54. 2002
    ....
  99. ncbi Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast
    Youn Uck Kim
    Department of Biological Sciences, Sun Moon University, A San City, Korea
    J Biochem 142:123-9. 2007
    ..This suggests that the association of PIG-X and PIG-M for GPI-MT-I activity is not interchangeable between mammals and the other lower eukaryotes...
  100. ncbi The GPI1 homologue from Plasmodium falciparum complements a Saccharomyces cerevisiae GPI1 anchoring mutant
    Hosam Shams-Eldin
    Medizinisches Zentrum fur Hygiene und Med Mikrobiologie, Institut fur Virologie, Philipps Universitat Marburg, Robert Koch Strasse 17, 35037, Marburg, Germany
    Mol Biochem Parasitol 120:73-81. 2002
    ..Hydrophobicity plots revealed multiple hydrophobic regions that could function as transmembrane segments. The cloned P. falciparum GPI1 gene complemented a gpi1 yeast mutant...
  101. ncbi [Paroxysmal nocturnal hemoglobinuria: mechanism of clonal expansion]
    Taroh Kinoshita
    Rinsho Ketsueki 46:317-23. 2005