Affiliation: Osaka University
Wang Y, Hirata T, Maeda Y, Murakami Y, Fujita M, Kinoshita T. Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited. J Biol Chem. 2019;294:5038-5049 pubmed publisher
..Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells. ..
Kinoshita T. Congenital Defects in the Expression of the Glycosylphosphatidylinositol-Anchored Complement Regulatory Proteins CD59 and Decay-Accelerating Factor. Semin Hematol. 2018;55:136-140 pubmed publisher
..Eculizumab, an anti-C5 antibody, is effective in relieving the symptoms seen in patients with complement dysregulation. ..
Kinoshita T. Designing sleeping sickness control. ACS Chem Biol. 2008;3:601-3 pubmed publisher
..An article in this issue reports the first chemically synthesized inhibitor of the third mannosyltransferase from the GPI pathway, stimulating further investigation toward practical and useful compounds. ..
Kinoshita T, Fujita M. Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling. J Lipid Res. 2016;57:6-24 pubmed publisher
..Yeast Saccharomyces cerevisiae has >60 GPI-anchored proteins (GPI-APs). GPI is essential for growth of yeast. In this review, we discuss biosynthesis of GPI-APs in mammalian cells and yeast with emphasis on the lipid moiety. ..
Liu Y, Guo X, Hirata T, Rong Y, Motooka D, Kitajima T, et al
. N-Glycan-dependent protein folding and endoplasmic reticulum retention regulate GPI-anchor processing. J Cell Biol. 2018;217:585-599 pubmed publisher
..Once the system is disrupted by ER stress, unprocessed GPI-APs become exposed on the cell surface. ..
Kinoshita T, Fujita M, Maeda Y. Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress. J Biochem. 2008;144:287-94 pubmed publisher
..It is thought that association with membrane rafts is important for GPI-APs in signal transduction and other functions. Here, we review recent progress in studies on biosynthesis, remodelling and functions of mammalian GPI-APs. ..
Murakami Y, Kinoshita T, Maeda Y, Nakano T, Kosaka H, Takeda J. Different roles of glycosylphosphatidylinositol in various hematopoietic cells as revealed by a mouse model of paroxysmal nocturnal hemoglobinuria. Blood. 1999;94:2963-70 pubmed
Kinoshita T, Inoue N. Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis. Curr Opin Chem Biol. 2000;4:632-8 pubmed
..These studies have revealed the common and also different characteristics of glycosylphosphatidyl-inositol biosynthesis enzymes in different organisms, leading to the development of species-specific inhibitors of the pathway. ..
Theiler R, Fujita M, Nagae M, Yamaguchi Y, Maeda Y, Kinoshita T. The ?-helical region in p24?2 subunit of p24 protein cargo receptor is pivotal for the recognition and transport of glycosylphosphatidylinositol-anchored proteins. J Biol Chem. 2014;289:16835-43 pubmed publisher