Research Topics
Genomes and Genes
| Kazuhiro IwaiSummaryAffiliation: Osaka University Country: Japan Publications
| Collaborators
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Detail Information
Publications
Involvement of linear polyubiquitylation of NEMO in NF-kappaB activationFuminori Tokunaga
Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
Nat Cell Biol 11:123-32. 2009..These results indicate that LUBAC is involved in the physiological regulation of the canonical NF-kappaB activation pathway through linear polyubiquitylation of NEMO...- Diverse ubiquitin signaling in NF-κB activationKazuhiro Iwai
Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
Trends Cell Biol 22:355-64. 2012..This review discusses existing evidence of the differential roles played by various ubiquitin chains, particularly K63, M1, and K11 chains, in NF-κB activation... 
Generation and physiological roles of linear ubiquitin chainsHenning Walczak
Tumour Immunology Unit, Department of Medicine, Imperial College London, 10N5 Commonwealth Building, Du Cane Road, London W12 0NN, UK
BMC Biol 10:23. 2012..We focus here on linear ubiquitin chains, how they are assembled, and how three different avenues of research have indicated physiological roles for linear ubiquitination in innate and adaptive immunity and suppression of inflammation...- Synthesis and analysis of linear ubiquitin chainsKazuhiro Iwai
Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
Methods Mol Biol 832:229-38. 2012..Linear polyubiquitin chains were shown to be involved in NF-κB activation. Here, we describe methods to synthesize linear polyubiquitin chains in vitro and to detect linear chains in vivo... - Linear polyubiquitin chains: a new modifier involved in NFκB activation and chronic inflammation, including dermatitisKazuhiro Iwai
Department of Biophysics and Biochemistry, Graduate School of Medicine, Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
Cell Cycle 10:3095-104. 2011..The pleomorphic symptoms of cpdm mice suggest that LUBAC-mediated NFκB activation may play critical roles in mammals and be involved in various disorders. A forward look into the linear polyubiquitin research is also discussed... - Linear polyubiquitination: a new regulator of NF-kappaB activationKazuhiro Iwai
Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology, Osaka University, Osaka, Japan
EMBO Rep 10:706-13. 2009..Future directions for linear polyubiquitin research are also discussed... - Functions of Linear Ubiquitin Chains in the NF-κB Pathway : Linear Polyubiquitin in NF-κB SignalingKazuhiro Iwai
Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
Subcell Biochem 54:100-6. 2010..In this chapter, we will discuss the distinct roles of linear and K63-linked ubiquitin chains in TNF-α mediated NF-κB activation and the future directions for linear ubiquitin chain research... 
Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complexKotaro Shirakawa
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
Virology 344:263-6. 2006..These results suggest that Vif exhibits its anti-APOBEC3 activity by the ubiquitin ligase activity of the Vif-BC-Cul5 complex...- A ubiquitin ligase complex assembles linear polyubiquitin chainsTakayoshi Kirisako
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka, Japan
EMBO J 25:4877-87. 2006..Moreover, the complex regulates the stability of Ub-GFP (a GFP fusion protein with an N-terminal ubiquitin). The linear polyubiquitin chain generated post-translationally may function as a new modulator of proteins... - SHARPIN is a component of the NF-κB-activating linear ubiquitin chain assembly complexFuminori Tokunaga
Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
Nature 471:633-6. 2011.... - Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppressionRyo Ueta
Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Mol Cell Biol 32:4998-5008. 2012.... - MDM2 is a novel E3 ligase for HIV-1 VifTaisuke Izumi
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo ku, Japan
Retrovirology 6:1. 2009..These data suggest that MDM2 is a regulator of HIV-1 replication and might be a novel therapeutic target for anti-HIV-1 drug... - Identification, expression, and assay of an oxidation-specific ubiquitin ligase, HOIL-1Kazuhiro Iwai
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, and Japan Science and Technology Agency, Osaka 545-8585, Japan
Methods Enzymol 398:256-71. 2005..This chapter describes the detailed methods used for the identification and functional analysis of the HOIL-1 ligase... - The COP9/signalosome increases the efficiency of von Hippel-Lindau protein ubiquitin ligase-mediated hypoxia-inducible factor-alpha ubiquitinationYasuhiro Miyauchi
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Abeno Ku, Osaka 545 8585, Japan
J Biol Chem 283:16622-31. 2008.... - Involvement of heme regulatory motif in heme-mediated ubiquitination and degradation of IRP2Haruto Ishikawa
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan
Mol Cell 19:171-81. 2005.... - Unusual heme binding in the bacterial iron response regulator protein: spectral characterization of heme binding to the heme regulatory motifHaruto Ishikawa
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615 8530, Japan
Biochemistry 50:1016-22. 2011..Therefore, we conclude that heme binding to HRM in the heme-regulated protein, Irr, is quite different from that in conventional hemoproteins but characteristic of heme-regulated proteins using heme as the signaling molecule... - Multiple roles of Rbx1 in the VBC-Cul2 ubiquitin ligase complexYuzuru Megumi
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
Genes Cells 10:679-91. 2005..Taken together, these results indicate that various mechanisms regulate both the activities and the stability of cullin-based ligases... - Mutual regulation of conventional protein kinase C and a ubiquitin ligase complexMunehiro Nakamura
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka 545 8585, Japan
Biochem Biophys Res Commun 351:340-7. 2006..These results indicate that LUBAC is an E3 for activated conventional PKC, and that PKC and LUBAC regulate each other for proper PKC signaling... - Gp78 cooperates with RMA1 in endoplasmic reticulum-associated degradation of CFTRDeltaF508Daisuke Morito
Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
Mol Biol Cell 19:1328-36. 2008..Our data demonstrates that gp78 cooperates with RMA1 with E4-like activity in the ERAD of CFTRDeltaF508... - Ubiquitination of APOBEC3G by an HIV-1 Vif-Cullin5-Elongin B-Elongin C complex is essential for Vif functionMasayuki Kobayashi
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
J Biol Chem 280:18573-8. 2005..These findings suggest that the E3 ubiquitin ligase activity of the Vif-BC-Cul5 complex is essential for Vif function against APOBEC3G... - Mechanism underlying the iron-dependent nuclear export of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiaeRyo Ueta
Department of Applied Molecular Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
Mol Biol Cell 18:2980-90. 2007.... - Distinct mechanisms of ferritin delivery to lysosomes in iron-depleted and iron-replete cellsTakeshi Asano
Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
Mol Cell Biol 31:2040-52. 2011.... - [The protein degradation system involved in the regulation of iron metabolism]Kazuhiro Iwai
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
Gan To Kagaku Ryoho 35:16-20. 2008.... - [Mechanism underlying iron metabolism: involvement of mitochondria in cellular iron sensing]Kazuhiro Iwai
Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan
Seikagaku 79:1021-31. 2007 - Activation of nuclear factor-kappa B by linear ubiquitin chain assembly complex contributes to lung metastasis of osteosarcoma cellsMasato Tomonaga
Department of Orthopedic Surgery, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
Int J Oncol 40:409-17. 2012.... - RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathwayKana Shimada
Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan
Biochem Biophys Res Commun 404:910-4. 2011..These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents... - Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulationFuminori Tokunaga
Laboratory of Molecular Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan
EMBO J 31:3856-70. 2012..These findings provide new insight into the regulation of immune and inflammatory responses... - Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2Koji Yamanaka
Department of Molecular and System Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Nat Cell Biol 5:336-40. 2003..The oxidation of IRP2 is generated by haem, which binds to IRP2 in iron-rich cells, and by oxygen, indicating that the iron sensing of IRP2 depends on the synthesis and availability of haem... - LUBAC, a novel ubiquitin ligase for linear ubiquitination, is crucial for inflammation and immune responsesFuminori Tokunaga
Laboratory of Molecular Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371 8512, Japan
Microbes Infect 14:563-72. 2012..Recent findings on the regulation of NF-κB by LUBAC and the diseases associated with this process are the focus of this review... - [Regulation of iron metabolism and its involvement in diseases]Kazuhiro Iwai
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
Rinsho Byori 56:517-24. 2008..Here I describe recent advance in our understanding on the regulation of iron metabolism and discuss its relationship to various diseases... - A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reactionMasaki Takatsuka
Department of Bacteriology, Osaka City University Graduate School of Medicine, Abeno Ku, Osaka, Japan
PLoS ONE 6:e20985. 2011..This study describes for the first time a ferroxidase-iron storage protein outside of the ferritin superfamily proteins and the protective role of this bacterial protein from DNA damage...