Kazuhiro Iwai

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. doi request reprint Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation
    Fuminori Tokunaga
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Nat Cell Biol 11:123-32. 2009
  2. doi request reprint Diverse ubiquitin signaling in NF-κB activation
    Kazuhiro Iwai
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Trends Cell Biol 22:355-64. 2012
  3. pmc Generation and physiological roles of linear ubiquitin chains
    Henning Walczak
    Tumour Immunology Unit, Department of Medicine, Imperial College London, 10N5 Commonwealth Building, Du Cane Road, London W12 0NN, UK
    BMC Biol 10:23. 2012
  4. doi request reprint Synthesis and analysis of linear ubiquitin chains
    Kazuhiro Iwai
    Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Methods Mol Biol 832:229-38. 2012
  5. pmc Linear polyubiquitination: a new regulator of NF-kappaB activation
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology, Osaka University, Osaka, Japan
    EMBO Rep 10:706-13. 2009
  6. pmc Linear polyubiquitin chains: a new modifier involved in NFκB activation and chronic inflammation, including dermatitis
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
    Cell Cycle 10:3095-104. 2011
  7. doi request reprint Functions of Linear Ubiquitin Chains in the NF-κB Pathway : Linear Polyubiquitin in NF-κB Signaling
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Subcell Biochem 54:100-6. 2010
  8. ncbi request reprint Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complex
    Kotaro Shirakawa
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
    Virology 344:263-6. 2006
  9. pmc A ubiquitin ligase complex assembles linear polyubiquitin chains
    Takayoshi Kirisako
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka, Japan
    EMBO J 25:4877-87. 2006
  10. doi request reprint SHARPIN is a component of the NF-κB-activating linear ubiquitin chain assembly complex
    Fuminori Tokunaga
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 471:633-6. 2011

Detail Information

Publications38

  1. doi request reprint Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation
    Fuminori Tokunaga
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Nat Cell Biol 11:123-32. 2009
    ..These results indicate that LUBAC is involved in the physiological regulation of the canonical NF-kappaB activation pathway through linear polyubiquitylation of NEMO...
  2. doi request reprint Diverse ubiquitin signaling in NF-κB activation
    Kazuhiro Iwai
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Trends Cell Biol 22:355-64. 2012
    ..This review discusses existing evidence of the differential roles played by various ubiquitin chains, particularly K63, M1, and K11 chains, in NF-κB activation...
  3. pmc Generation and physiological roles of linear ubiquitin chains
    Henning Walczak
    Tumour Immunology Unit, Department of Medicine, Imperial College London, 10N5 Commonwealth Building, Du Cane Road, London W12 0NN, UK
    BMC Biol 10:23. 2012
    ..We focus here on linear ubiquitin chains, how they are assembled, and how three different avenues of research have indicated physiological roles for linear ubiquitination in innate and adaptive immunity and suppression of inflammation...
  4. doi request reprint Synthesis and analysis of linear ubiquitin chains
    Kazuhiro Iwai
    Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Methods Mol Biol 832:229-38. 2012
    ..Linear polyubiquitin chains were shown to be involved in NF-κB activation. Here, we describe methods to synthesize linear polyubiquitin chains in vitro and to detect linear chains in vivo...
  5. pmc Linear polyubiquitination: a new regulator of NF-kappaB activation
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology, Osaka University, Osaka, Japan
    EMBO Rep 10:706-13. 2009
    ..Future directions for linear polyubiquitin research are also discussed...
  6. pmc Linear polyubiquitin chains: a new modifier involved in NFκB activation and chronic inflammation, including dermatitis
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
    Cell Cycle 10:3095-104. 2011
    ..The pleomorphic symptoms of cpdm mice suggest that LUBAC-mediated NFκB activation may play critical roles in mammals and be involved in various disorders. A forward look into the linear polyubiquitin research is also discussed...
  7. doi request reprint Functions of Linear Ubiquitin Chains in the NF-κB Pathway : Linear Polyubiquitin in NF-κB Signaling
    Kazuhiro Iwai
    Department of Biophysics and Biochemistry, Graduate School of Medicine and Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Subcell Biochem 54:100-6. 2010
    ..In this chapter, we will discuss the distinct roles of linear and K63-linked ubiquitin chains in TNF-α mediated NF-κB activation and the future directions for linear ubiquitin chain research...
  8. ncbi request reprint Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complex
    Kotaro Shirakawa
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
    Virology 344:263-6. 2006
    ..These results suggest that Vif exhibits its anti-APOBEC3 activity by the ubiquitin ligase activity of the Vif-BC-Cul5 complex...
  9. pmc A ubiquitin ligase complex assembles linear polyubiquitin chains
    Takayoshi Kirisako
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka, Japan
    EMBO J 25:4877-87. 2006
    ..Moreover, the complex regulates the stability of Ub-GFP (a GFP fusion protein with an N-terminal ubiquitin). The linear polyubiquitin chain generated post-translationally may function as a new modulator of proteins...
  10. doi request reprint SHARPIN is a component of the NF-κB-activating linear ubiquitin chain assembly complex
    Fuminori Tokunaga
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 471:633-6. 2011
    ....
  11. pmc Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression
    Ryo Ueta
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Mol Cell Biol 32:4998-5008. 2012
    ....
  12. pmc MDM2 is a novel E3 ligase for HIV-1 Vif
    Taisuke Izumi
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo ku, Japan
    Retrovirology 6:1. 2009
    ..These data suggest that MDM2 is a regulator of HIV-1 replication and might be a novel therapeutic target for anti-HIV-1 drug...
  13. ncbi request reprint Identification, expression, and assay of an oxidation-specific ubiquitin ligase, HOIL-1
    Kazuhiro Iwai
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, and Japan Science and Technology Agency, Osaka 545 8585, Japan
    Methods Enzymol 398:256-71. 2005
    ..This chapter describes the detailed methods used for the identification and functional analysis of the HOIL-1 ligase...
  14. doi request reprint The COP9/signalosome increases the efficiency of von Hippel-Lindau protein ubiquitin ligase-mediated hypoxia-inducible factor-alpha ubiquitination
    Yasuhiro Miyauchi
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Abeno Ku, Osaka 545 8585, Japan
    J Biol Chem 283:16622-31. 2008
    ....
  15. ncbi request reprint IFN-γ or IFN-α ameliorates chronic proliferative dermatitis by inducing expression of linear ubiquitin chain assembly complex
    Hironari Tamiya
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Immunol 192:3793-804. 2014
    ..These results indicate that the IFNs enhance NF-κB activation and ameliorate cpdm dermatitis by augmenting expression of HOIP and HOIL-1L and linear polyubiquitination activity of LUBAC. ..
  16. ncbi request reprint Involvement of heme regulatory motif in heme-mediated ubiquitination and degradation of IRP2
    Haruto Ishikawa
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka 545 8585, Japan
    Mol Cell 19:171-81. 2005
    ....
  17. pmc Unusual heme binding in the bacterial iron response regulator protein: spectral characterization of heme binding to the heme regulatory motif
    Haruto Ishikawa
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615 8530, Japan
    Biochemistry 50:1016-22. 2011
    ..Therefore, we conclude that heme binding to HRM in the heme-regulated protein, Irr, is quite different from that in conventional hemoproteins but characteristic of heme-regulated proteins using heme as the signaling molecule...
  18. ncbi request reprint Multiple roles of Rbx1 in the VBC-Cul2 ubiquitin ligase complex
    Yuzuru Megumi
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    Genes Cells 10:679-91. 2005
    ..Taken together, these results indicate that various mechanisms regulate both the activities and the stability of cullin-based ligases...
  19. pmc Defective immune responses in mice lacking LUBAC-mediated linear ubiquitination in B cells
    Yoshiteru Sasaki
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    EMBO J 32:2463-76. 2013
    ....
  20. ncbi request reprint Mechanism underlying IκB kinase activation mediated by the linear ubiquitin chain assembly complex
    Hiroaki Fujita
    Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Mol Cell Biol 34:1322-35. 2014
    ....
  21. ncbi request reprint Mutual regulation of conventional protein kinase C and a ubiquitin ligase complex
    Munehiro Nakamura
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka 545 8585, Japan
    Biochem Biophys Res Commun 351:340-7. 2006
    ..These results indicate that LUBAC is an E3 for activated conventional PKC, and that PKC and LUBAC regulate each other for proper PKC signaling...
  22. pmc Gp78 cooperates with RMA1 in endoplasmic reticulum-associated degradation of CFTRDeltaF508
    Daisuke Morito
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    Mol Biol Cell 19:1328-36. 2008
    ..Our data demonstrates that gp78 cooperates with RMA1 with E4-like activity in the ERAD of CFTRDeltaF508...
  23. ncbi request reprint Suppression of LUBAC-mediated linear ubiquitination by a specific interaction between LUBAC and the deubiquitinases CYLD and OTULIN
    Tsuyoshi Takiuchi
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan Department of Obstetrics and Gynecology, Graduate School of Medicine, Osaka University, Suita, Osaka, 565 0871, Japan
    Genes Cells 19:254-72. 2014
    ..Our observations provide molecular insights into the roles of ligase-deubiquitinase interactions in regulating molecular events resulting from linear ubiquitin conjugation. ..
  24. pmc Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin
    Naonobu Fujita
    Department of Genetics, Graduate School of Medicine, 2 Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, 3 Department of Molecular Virology, Research Institute for Microbial Diseases, 4 Department of Host Defense, WPI Immunology Frontier Research Center, 5 Department of Host Defense, Research Institute for Microbial Disease, and 6 Core Instrumentation Facility, Research Institute for Microbial Disease, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Cell Biol 203:115-28. 2013
    ..Thus, we reveal that ubiquitin is a pivotal molecule that connects bacteria-containing endosomes with the autophagic machinery upstream of LC3. ..
  25. pmc IOP1 protein is an external component of the human cytosolic iron-sulfur cluster assembly (CIA) machinery and functions in the MMS19 protein-dependent CIA pathway
    Mineaki Seki
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 288:16680-9. 2013
    ..We also reveal that MMS19 interacts with target proteins. MIP18 has a role to bridge MMS19 and CIAO1. CIAO1 also binds IOP1. Based on our in vivo and in vitro data, new models of the CIA machinery are proposed...
  26. ncbi request reprint Ubiquitination of APOBEC3G by an HIV-1 Vif-Cullin5-Elongin B-Elongin C complex is essential for Vif function
    Masayuki Kobayashi
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
    J Biol Chem 280:18573-8. 2005
    ..These findings suggest that the E3 ubiquitin ligase activity of the Vif-BC-Cul5 complex is essential for Vif function against APOBEC3G...
  27. pmc Distinct mechanisms of ferritin delivery to lysosomes in iron-depleted and iron-replete cells
    Takeshi Asano
    Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 31:2040-52. 2011
    ....
  28. pmc Mechanism underlying the iron-dependent nuclear export of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae
    Ryo Ueta
    Department of Applied Molecular Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
    Mol Biol Cell 18:2980-90. 2007
    ....
  29. ncbi request reprint [The protein degradation system involved in the regulation of iron metabolism]
    Kazuhiro Iwai
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    Gan To Kagaku Ryoho 35:16-20. 2008
    ....
  30. ncbi request reprint [Mechanism underlying iron metabolism: involvement of mitochondria in cellular iron sensing]
    Kazuhiro Iwai
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    Seikagaku 79:1021-31. 2007
  31. doi request reprint Activation of nuclear factor-kappa B by linear ubiquitin chain assembly complex contributes to lung metastasis of osteosarcoma cells
    Masato Tomonaga
    Department of Orthopedic Surgery, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
    Int J Oncol 40:409-17. 2012
    ....
  32. doi request reprint RPAP3 enhances cytotoxicity of doxorubicin by impairing NF-kappa B pathway
    Kana Shimada
    Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan
    Biochem Biophys Res Commun 404:910-4. 2011
    ..These results indicate that RPAP3 may be a novel modulator of NF-κB pathway in apoptosis induced by anti-cancer chemotherapeutic agents...
  33. pmc Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulation
    Fuminori Tokunaga
    Laboratory of Molecular Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan
    EMBO J 31:3856-70. 2012
    ..These findings provide new insight into the regulation of immune and inflammatory responses...
  34. doi request reprint Diverse roles of the ubiquitin system in NF-κB activation
    Kazuhiro Iwai
    Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan Electronic address
    Biochim Biophys Acta 1843:129-36. 2014
    ..This article is part of a Special Issue entitled: Ubiquitin-Proteasome System. Guest Editors: Thomas Sommer and Dieter H. Wolf. ..
  35. ncbi request reprint Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2
    Koji Yamanaka
    Department of Molecular and System Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 5:336-40. 2003
    ..The oxidation of IRP2 is generated by haem, which binds to IRP2 in iron-rich cells, and by oxygen, indicating that the iron sensing of IRP2 depends on the synthesis and availability of haem...
  36. doi request reprint LUBAC, a novel ubiquitin ligase for linear ubiquitination, is crucial for inflammation and immune responses
    Fuminori Tokunaga
    Laboratory of Molecular Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371 8512, Japan
    Microbes Infect 14:563-72. 2012
    ..Recent findings on the regulation of NF-κB by LUBAC and the diseases associated with this process are the focus of this review...
  37. pmc A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reaction
    Masaki Takatsuka
    Department of Bacteriology, Osaka City University Graduate School of Medicine, Abeno Ku, Osaka, Japan
    PLoS ONE 6:e20985. 2011
    ..This study describes for the first time a ferroxidase-iron storage protein outside of the ferritin superfamily proteins and the protective role of this bacterial protein from DNA damage...
  38. ncbi request reprint [Regulation of iron metabolism and its involvement in diseases]
    Kazuhiro Iwai
    Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Rinsho Byori 56:517-24. 2008
    ..Here I describe recent advance in our understanding on the regulation of iron metabolism and discuss its relationship to various diseases...