Masahito Ikawa


Affiliation: Osaka University
Country: Japan


  1. Miyata H, Castaneda J, Fujihara Y, Yu Z, Archambeault D, Isotani A, et al. Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016;113:7704-10 pubmed publisher
  2. Fujihara Y, Oji A, Kojima Kita K, Larasati T, Ikawa M. Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice. J Cell Sci. 2018;131: pubmed publisher
    ..We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability. ..
  3. Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, et al. RSPH6A is required for sperm flagellum formation and male fertility in mice. J Cell Sci. 2018;131: pubmed publisher
    ..These data indicate that RSPH6A is essential for sperm flagellar assembly and male fertility in mice.This article has an associated First Person interview with the first author of the paper. ..
  4. Satouh Y, Ikawa M. New Insights into the Molecular Events of Mammalian Fertilization. Trends Biochem Sci. 2018;: pubmed publisher
  5. Fujihara Y, Ikawa M. CRISPR/Cas9-based genome editing in mice by single plasmid injection. Methods Enzymol. 2014;546:319-36 pubmed publisher
    ..These mice can be generated within a month. ..
  6. Fujihara Y, Ikawa M. GPI-AP release in cellular, developmental, and reproductive biology. J Lipid Res. 2016;57:538-45 pubmed publisher
    ..Here, the protein modifications resulting from the cleavage of the GPI anchor are discussed in the context of its physiological functions. ..
  7. Nozawa K, Satouh Y, Fujimoto T, Oji A, Ikawa M. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep. 2018;8:1315 pubmed publisher
    ..These results suggest that mammalian spermatozoa have a primitive oocyte activation mechanism and that PLCζ1 is a SOAF that ensures oocyte activation steps for monospermic fertilization in mammals. ..
  8. Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, et al. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015;16:274 pubmed publisher
    ..Further, the i-PITT system is freely accessible to scientific community. ..
  9. Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, et al. Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 2015;350:442-5 pubmed publisher
    ..Because human spermatozoa contain PPP3CC and PPP3R2 as a form of calcineurin, inhibition of this sperm-specific calcineurin may lead to the development of a reversible male contraceptive that would target spermatozoa in the epididymis. ..

More Information


  1. request reprint
    Young S, Miyata H, Satouh Y, Muto M, Larsen M, Aitken R, et al. CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility. Reproduction. 2016;152:665-672 pubmed
  2. Tobita T, Kiyozumi D, Ikawa M. Placenta-specific gene manipulation using lentiviral vector and its application. Placenta. 2017;59 Suppl 1:S37-S43 pubmed publisher
    ..A perspective for future application of LV vectors to further placenta research, especially in combination with next generation genome editing technologies, is also presented. ..
  3. Ikawa M, Tokuhiro K, Yamaguchi R, Benham A, Tamura T, Wada I, et al. Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011;286:5639-46 pubmed publisher
    ..The identification and understanding of the maturation mechanisms of key sperm proteins will pave the way toward novel approaches for both contraception and treatment of unexplained male infertility...
  4. Young S, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, et al. CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int J Mol Sci. 2015;16:24732-50 pubmed publisher
    ..This study demonstrates the versatility of the CRISPR/Cas9 system to generate animal models of a highly complex system by introducing point mutations and simple knockouts in a fast and efficient manner. ..
  5. Muto M, Fujihara Y, Tobita T, Kiyozumi D, Ikawa M. Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice. Biol Reprod. 2016;94:6 pubmed publisher
    ..Our findings suggest that Plac1 is involved in trophoblast cell proliferation, differentiation, and migration. Its proper expression is required for normal placentation and fetal development. ..
  6. request reprint
    Young S, Miyata H, Satouh Y, Aitken R, Baker M, Ikawa M. CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. J Cell Sci. 2016;129:4379-4387 pubmed
    ..Our results show that it is the role of CABYR in the formation of the fibrous sheath that is essential for male fertility. ..
  7. Noda T, Oji A, Ikawa M. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Methods Mol Biol. 2017;1630:67-80 pubmed publisher
    ..Thus, we recently established the system for genome editing in embryonic stem (ES) cells and its application for chimeric analysis in mice. In this section, we introduce the procedure for genome editing in mouse zygotes and ES cells. ..
  8. Fujihara Y, Oji A, Larasati T, Kojima Kita K, Ikawa M. Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Int J Mol Sci. 2017;18: pubmed publisher
    ..This study demonstrates that Spata16 is indispensable for male fertility in mice, as well as in humans, as revealed by CRISPR/Cas9-mediated mouse models. ..
  9. Satouh Y, Nozawa K, Ikawa M. Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation. Biol Reprod. 2015;93:94 pubmed publisher
    ..We conclude that PAWP does not play an essential role in mouse fertilization. ..