Masaki Magari

Summary

Affiliation: Okayama University
Country: Japan

Publications

  1. pmc In vitro substrate phosphorylation by Ca²⁺/calmodulin-dependent protein kinase kinase using guanosine-5'-triphosphate as a phosphate donor
    Saki Yurimoto
    Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, 1750 1 Miki cho, Kita gun, Kagawa 761 0793, Japan
    BMC Biochem 13:27. 2012
  2. pmc Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line
    Naoki Kanayama
    Department of Biotechnology, Okayama University, Tsushima Naka, 3 1 1, Okayama 700 8530, Japan
    Nucleic Acids Res 34:e10. 2006
  3. ncbi request reprint [Creation of valuable antibodies by an in vitro antibody generation system using a hypermutating B cell line]
    Naoki Kanayama
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1, Tsushima Naka Okayama 700 8530, Japan
    Yakugaku Zasshi 129:11-7. 2009
  4. doi request reprint Analysis of B cell selection in the germinal center reaction during a T-dependent antibody response at a single cell level
    Takahiro Okazawa
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Immunol Lett 117:96-105. 2008
  5. ncbi request reprint Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone
    Naoki Kanayama
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama, Japan
    J Immunol 174:1438-45. 2005
  6. doi request reprint Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
    Masamichi Kajita
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Kita ku, Okayama 700 8530, Japan
    J Biosci Bioeng 109:407-10. 2010
  7. ncbi request reprint Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire
    Masaki Magari
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Biochem Biophys Res Commun 396:353-8. 2010
  8. ncbi request reprint B cell selection and affinity maturation during an antibody response in the mouse with limited B cell diversity
    Naoki Kanayama
    Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama, Japan
    J Immunol 169:6865-74. 2002
  9. ncbi request reprint Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library
    Kagefumi Todo
    Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    J Biosci Bioeng 102:478-81. 2006
  10. doi request reprint IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells
    Masaki Magari
    Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Kita ku, Okayama 700 8530, Japan
    J Immunol 187:4210-8. 2011

Collaborators

  • Naoki Kanayama
  • Takafumi Kimoto
  • Matthias Wabl
  • Hiroshi Kitamura
  • Michael Reth
  • Tomohiro Kurosaki
  • M Cascalho
  • Hitoshi Ohmori
  • Kagefumi Todo
  • Masamichi Kajita
  • Takahiro Okazawa
  • Yumiko Nishikawa
  • Kazunori Kataoka
  • Nobuhiro Nishiyama
  • Emi Kouyama
  • Yuichi Kanehiro
  • Yuichi Yamasaki
  • Saki Yurimoto
  • Keiji Itaka
  • Makoto Oba
  • - Arnida
  • Woo Dong Jang
  • Masaki Hikida
  • Daisuke Wakebayashi
  • Misaki Negishi
  • Hiroshi Tokumitsu
  • Tomohito Fujimoto
  • Junji Fukuoka
  • Wenjian Gan
  • Xialu Li
  • Takuya Hikasa
  • James L Manley
  • Ryoji Kobayashi
  • Masayuki Takemoto
  • Yoshiaki Kaga
  • Mika Ikeda
  • Hiroyuki Koyama
  • Shigeto Fukushima
  • Kazuhiro Aoyagi
  • Masaharu Mori
  • Kenji Miyake
  • Masahiro Takeuchi
  • Michio Oka
  • Hiroyuki Shigemitsu
  • Atsushi Harada
  • Kozo Nakamura
  • Yukio Nagasaki
  • Hiroshi Kawaguchi
  • Yasunori Nakayama

Detail Information

Publications27

  1. pmc In vitro substrate phosphorylation by Ca²⁺/calmodulin-dependent protein kinase kinase using guanosine-5'-triphosphate as a phosphate donor
    Saki Yurimoto
    Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, 1750 1 Miki cho, Kita gun, Kagawa 761 0793, Japan
    BMC Biochem 13:27. 2012
    ..To identify previously unidentified CaMKK substrates, we used various nucleotides as phosphate donors to develop and characterize an in vitro phosphorylation assay for CaMKK...
  2. pmc Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line
    Naoki Kanayama
    Department of Biotechnology, Okayama University, Tsushima Naka, 3 1 1, Okayama 700 8530, Japan
    Nucleic Acids Res 34:e10. 2006
    ..Thus, the gene conversion machinery in DT40 cells will be a useful means to engineer non-Ig proteins by a type of DNA shuffling...
  3. ncbi request reprint [Creation of valuable antibodies by an in vitro antibody generation system using a hypermutating B cell line]
    Naoki Kanayama
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1, Tsushima Naka Okayama 700 8530, Japan
    Yakugaku Zasshi 129:11-7. 2009
    ..We have also genetically improved DT40-SW cells for mutation efficiency and Ab production. The Ab generation system will be applicable for obtaining valuable Abs such as antitumor Abs...
  4. doi request reprint Analysis of B cell selection in the germinal center reaction during a T-dependent antibody response at a single cell level
    Takahiro Okazawa
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Immunol Lett 117:96-105. 2008
    ..Thus, it is suggested that B cells with a higher affinity were selected not only for entry to the GCs but also in the affinity maturation process during a T-dependent Ab response...
  5. ncbi request reprint Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone
    Naoki Kanayama
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama, Japan
    J Immunol 174:1438-45. 2005
    ..Taken together, it is suggested that polyreactivity (including self-reactivity) of BCR is crucial in driving B cells to differentiate into the MZ phenotype...
  6. doi request reprint Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
    Masamichi Kajita
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Kita ku, Okayama 700 8530, Japan
    J Biosci Bioeng 109:407-10. 2010
    ..To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression...
  7. ncbi request reprint Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire
    Masaki Magari
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Biochem Biophys Res Commun 396:353-8. 2010
    ..Thus, DT40-SWDeltaC cells may be useful for constructing Ab libraries for efficient screening of mAbs in vitro...
  8. ncbi request reprint B cell selection and affinity maturation during an antibody response in the mouse with limited B cell diversity
    Naoki Kanayama
    Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama, Japan
    J Immunol 169:6865-74. 2002
    ..Thus, even weak B cell receptor engagement can elicit an IgM response, whereas only B cells that received signals stronger than a threshold may be committed to an affinity maturation process...
  9. ncbi request reprint Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library
    Kagefumi Todo
    Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    J Biosci Bioeng 102:478-81. 2006
    ....
  10. doi request reprint IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells
    Masaki Magari
    Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Kita ku, Okayama 700 8530, Japan
    J Immunol 187:4210-8. 2011
    ..Taken together, results implicate a physiological role of IL-21/PGE(2)-induced B cell death in GC B cell selection...
  11. ncbi request reprint Analysis of antigen-stimulated B cell migration into germinal centers during the early stage of a T-dependent immune response
    Emi Kouyama
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Immunol Lett 109:28-35. 2007
    ..Thus, it is suggested that the higher BCR affinity of V(H)T/lambda2 B cells may be an initial cue for their recruitment to GCs during a T-dependent Ab response...
  12. doi request reprint Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation
    Masamichi Kajita
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    J Biosci Bioeng 110:351-8. 2010
    ..Taken together, hemizygous disruption of the XRCC3 gene is considered to be useful for obtaining high-affinity mAbs from DT40-SW cells though affinity maturation...
  13. ncbi request reprint Establishment of lymphotoxin beta receptor signaling-dependent cell lines with follicular dendritic cell phenotypes from mouse lymph nodes
    Yumiko Nishikawa
    Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka, Okayama, Japan
    J Immunol 177:5204-14. 2006
    ..Thus, pFL and FL-Y cells may be useful for providing insight into the functional role for FDCs in GC...
  14. pmc Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation requires a splice isoform of the serine/arginine-rich (SR) protein SRSF1
    Yuichi Kanehiro
    Department of Bioscience and Biotechnology, Okayama University Graduate School of Natural Science and Technology, Tsushima Naka, Kita ku, Okayama 700 8530, Japan
    Proc Natl Acad Sci U S A 109:1216-21. 2012
    ..Our findings, thus, define an unexpected and important role for SRSF1, particularly for its splice variant, in enabling AID to function specifically on its natural substrate during SHM...
  15. doi request reprint Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression
    Masaki Magari
    Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    J Biosci Bioeng 107:206-9. 2009
    ..This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density...
  16. ncbi request reprint Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line
    Naoki Kanayama
    Department of Biotechnology, Okayama University, Tsushima naka 3 1 1, Okayama 700 8530, Japan
    Biochem Biophys Res Commun 327:70-5. 2005
    ..Starting from a single DT40-SW cell, Ig gene repertoire was efficiently diversified during culture only when AID expression was on...
  17. ncbi request reprint Immunogenicity of an inflammation-associated product, tyrosine nitrated self-proteins
    Hitoshi Ohmori
    Department of Bioscience and Biotechnology, Okayama University, Tsushima naka 3 1 1, Okayama 700 8530, Japan
    Autoimmun Rev 4:224-9. 2005
    ..Thus, NT-containing epitopes created on self-proteins may serve as a trigger to impair or bypass immunological tolerance...
  18. ncbi request reprint Role for complement receptors (CD21/CD35) in the regulation of recombination activating gene expression in murine peripheral B cells
    Hitoshi Ohmori
    Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Japan
    Immunol Lett 83:95-9. 2002
    ..On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of BCRs in peripheral B cells...
  19. ncbi request reprint Suppressive effects of mometasone furoate on an antigen-specific IgE antibody response and production of IL-4 in mice
    Masaki Magari
    Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan
    Immunopharmacol Immunotoxicol 28:491-500. 2006
    ..Further, MF was effective in inhibiting production of type-2 helper T cell cytokines in vivo and in vitro. These results provide a immunopharmacological basis for clinical efficacy of this drug...
  20. ncbi request reprint Immunogenicity of autologous IgG bearing the inflammation-associated marker 3-nitrotyrosine
    Hitoshi Ohmori
    Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima Naka, Okayama 700 8530, Japan
    Immunol Lett 96:47-54. 2005
    ..Collectively, NT-IgG is immunogenic in autologous hosts, and can induce anti-NT Abs that are cross-reactive with ssDNA...
  21. ncbi request reprint Contribution of light chain rearrangement in peripheral B cells to the generation of high-affinity antibodies
    Masaki Magari
    Department of Biotechnology, Faculty of Engineering, Okayama University, Okayama, Japan
    Eur J Immunol 32:957-66. 2002
    ..Thus, our findings suggest that L chain rearrangement that occurs in the periphery can contribute to affinity maturation of Ab...
  22. ncbi request reprint Mechanisms leading to autoantibody production: link between inflammation and autoimmunity
    Hitoshi Ohmori
    Department of Biotechnology, Okayama University, Tsushima Naka, Okayama, 700 8530 Japan
    Curr Drug Targets Inflamm Allergy 2:232-41. 2003
    ..Investigations on the link between inflammation and autoimmunity will provide new aspects for therapeutic targets in the treatment of autoimmune diseases...
  23. doi request reprint Affinity capillary electrophoretic DNA separation using PEG-oligodeoxyribonucleotide block copolymers: relationship between peak resolution and affinity strength
    Naoki Kanayama
    Bioengineering Laboratory, RIKEN, Saitama, Japan Fax 81 48 462 4658
    J Sep Sci 31:837-44. 2008
    ..The base sequence of affinity probe ODN appropriate to achieve the sufficient resolution will be predicted on the basis of the mu(C) and K(d )values...
  24. ncbi request reprint Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing
    Keiji Itaka
    Department of Materials Science and Engineering, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Am Chem Soc 126:13612-3. 2004
    ..This design provided the carrier with a sufficient siRNA complexation and an assumed buffering capacity in the endosomes, allowing it to exhibit remarkable gene knockdown abilities as well as sufficient serum tolerability...
  25. ncbi request reprint Lactose-conjugated polyion complex micelles incorporating plasmid DNA as a targetable gene vector system: their preparation and gene transfecting efficiency against cultured HepG2 cells
    Daisuke Wakebayashi
    Department of Materials Science and Engineering, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo, Tokyo 113 8656, Japan
    J Control Release 95:653-64. 2004
    ....
  26. ncbi request reprint Cyclic RGD peptide-conjugated polyplex micelles as a targetable gene delivery system directed to cells possessing alphavbeta3 and alphavbeta5 integrins
    Makoto Oba
    Department of Clinical Vascular Regeneration, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo, Tokyo 113 8655, Japan
    Bioconjug Chem 18:1415-23. 2007
    ....
  27. ncbi request reprint PEGylated gene nanocarriers based on block catiomers bearing ethylenediamine repeating units directed to remarkable enhancement of photochemical transfection
    - Arnida
    Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Control Release 115:208-15. 2006
    ..With careful balance between photochemical transfection enhancement and cytotoxicity, PEG-b-polycatiomers used in this study might be a potential candidate for in vivo PCI-mediated gene transfer...