Naoya Kobayashi

Summary

Affiliation: Okayama University
Country: Japan

Publications

  1. ncbi request reprint Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:387-92. 2001
  2. doi request reprint Life support of artificial liver: development of a bioartificial liver to treat liver failure
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    J Hepatobiliary Pancreat Surg 16:113-7. 2009
  3. ncbi request reprint Cancer stem cell research: current situation and problems
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:19-25. 2008
  4. ncbi request reprint Bioartificial pancreas for the treatment of diabetes
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:11-7. 2008
  5. ncbi request reprint Artificial cells for the development of cell therapy
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:3-9. 2008
  6. ncbi request reprint Engineering of human hepatocyte lines for cell therapies in humans: prospects and remaining hurdles
    Naoya Kobayashit
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan
    Cell Transplant 11:417-20. 2002
  7. ncbi request reprint Cell transplantation. Introduction
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 17:1-2. 2008
  8. ncbi request reprint Cre/loxP-based reversible immortalization of human hepatocytes
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:383-6. 2001
  9. ncbi request reprint Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:377-81. 2001
  10. ncbi request reprint Insertion of a suicide gene into an immortalized human hepatocyte cell line
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:373-6. 2001

Collaborators

Detail Information

Publications122 found, 100 shown here

  1. ncbi request reprint Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:387-92. 2001
    ..These microspheres satisfied immediate preparation of NKNT-3 cells in sufficient quantity and of adequate quality...
  2. doi request reprint Life support of artificial liver: development of a bioartificial liver to treat liver failure
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    J Hepatobiliary Pancreat Surg 16:113-7. 2009
    ..We are also working on the development of a bioartificial liver (BAL) using such cells as a source. Herein, we introduce our findings on the current status of BAL development...
  3. ncbi request reprint Cancer stem cell research: current situation and problems
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:19-25. 2008
    ..Therefore, in the near future we hope that revolution will occur in cancer therapy to eradicate cancer and prevent the recurrence thereof. In this review we discuss the current situation and problems of cancer stem cell research...
  4. ncbi request reprint Bioartificial pancreas for the treatment of diabetes
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:11-7. 2008
    ..Various types of BAP devices have been developed and examined in animals. In this review, I introduce the previous BAP studies and our approach of BAP development...
  5. ncbi request reprint Artificial cells for the development of cell therapy
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 17:3-9. 2008
    ..I would like to further contribute toward the realization of tissue engineering in fusional coordination with cell-processing technology by the utilization of such cell line constructing techniques...
  6. ncbi request reprint Engineering of human hepatocyte lines for cell therapies in humans: prospects and remaining hurdles
    Naoya Kobayashit
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan
    Cell Transplant 11:417-20. 2002
    ..Such technology can allow rapid preparation of functional and uniform human hepatocytes. Here we present our strategy to construct transplantable human hepatocyte cell lines...
  7. ncbi request reprint Cell transplantation. Introduction
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 17:1-2. 2008
  8. ncbi request reprint Cre/loxP-based reversible immortalization of human hepatocytes
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:383-6. 2001
    ..Here we represent that elimination of the retrovirally transferred SV40Tag gene can be excised by adenovirus-mediated site-specific recombination...
  9. ncbi request reprint Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:377-81. 2001
    ..We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality...
  10. ncbi request reprint Insertion of a suicide gene into an immortalized human hepatocyte cell line
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    Cell Transplant 10:373-6. 2001
    ..We have established a tightly regulated immortalized human hepatocyte cell line. Essentially unlimited availability of OUMS-29/tk cells may be clinically useful for BAL therapy...
  11. ncbi request reprint Gene delivery to embryonic stem cells
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Birth Defects Res C Embryo Today 75:10-8. 2005
    ....
  12. ncbi request reprint Evaluation of toll-like receptor 4 gene expression of immortalized human liver cell lines
    N Kobayashi
    Division of Gastroenterology I, Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan
    Transplant Proc 35:431-2. 2003
  13. ncbi request reprint The current situation in human pancreatic islet transplantation: problems and prospects
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
    J Artif Organs 7:1-8. 2004
    ..Here we present general information on islet transplantation and discuss its problems and prospects...
  14. ncbi request reprint Transduction of human islets with the lentiviral vector
    N Kobayashi
    Department of Surgery, Okayama University Medical School, Okayama, Japan
    Transplant Proc 36:2203-4. 2004
    ..804G extracellular matrices were applied for monolayer formation of islets. Detection of NLS/LacZ expression was performed using X-gal staining. Lentiviral transduction was effective in these monolayer islets...
  15. ncbi request reprint Partial hepatectomy and subsequent radiation facilitates engraftment of mouse embryonic stem cells in the liver
    N Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplant Proc 36:2352-4. 2004
    ..A liver modified by PHT and RT facilitated engraftment of mouse ES cells compared with a normal liver. Hepatic RT significantly suppressed hepatocytic uptake of BrdU...
  16. ncbi request reprint Cell choice for bioartificial livers
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplantation, and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Keio J Med 52:151-7. 2003
    ....
  17. ncbi request reprint Hybrid bioartificial liver: establishing a reversibly immortalized human hepatocyte line and developing a bioartificial liver for practical use
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplantation, and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
    J Artif Organs 6:236-44. 2003
    ..The objective of this review article is to report our therapeutic strategy, which aims at the earliest possible introduction of the treatment of liver failure using BALs...
  18. ncbi request reprint Development of a cellulose-based microcarrier containing cellular adhesive peptides for a bioartificial liver
    N Kobayashi
    Department of Surgery, Okayama University Medical School, Okayama, Japan
    Transplant Proc 35:443-4. 2003
  19. ncbi request reprint Regenerative medicine for diabetes mellitus
    Naoya Kobayashi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 18:491-6. 2009
    ..The development of pancreatic beta-cell-targeting regenerative medicine can lead to the next generation of diabetes treatment...
  20. ncbi request reprint Active expression of p21 facilitates differentiation of immortalized human hepatocytes
    N Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplant Proc 35:433-4. 2003
  21. ncbi request reprint Cell therapy for diabetes mellitus
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:849-54. 2006
    ..In this article, I discuss the hurdles of the current therapy for diabetes and introduce the possible future treatment of diabetes...
  22. ncbi request reprint Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT
    Toshihisa Matsumura
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 77:1357-65. 2004
    ..We also demonstrate excision of SV40T and hTERT with TAT-mediated Cre/loxP recombination and subsequent cell sorting...
  23. ncbi request reprint Cryopreservation of primarily isolated porcine hepatocytes with UW solution
    Takemi Kunieda
    Division of Gastroenterology I, Department of Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701 0192, Japan
    Cell Transplant 12:607-16. 2003
    ..Intrasplenic transplantation of UW solution-cryopreserved hepatocytes improved the survival of rats treated with D-galactosamine. UW solution maintained the functions of cryopreserved porcine hepatocytes...
  24. ncbi request reprint Prolonged survival of mice with acute liver failure with transplantation of monkey hepatocytes cultured with an antiapoptotic pentapeptide V5
    Kimiaki Tanaka
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 81:427-37. 2006
    ..We here report the usefulness of an antiapoptotic pentapeptide V5, composed of Val-Pro-Met-Leu-Lys, in the monkey hepatocyte cultures...
  25. doi request reprint Establishment of an immortalized porcine liver cell line JSNK-1 with retroviral transduction of SV40T
    Javed M Shahid
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 19:849-56. 2010
    ..In conclusion, JSNK-1 cells would be valuable in the study of liver stellate cell pathophysiology and contribute to the optimization of hepatic differentiation of iPS cells...
  26. ncbi request reprint Maintenance of glucose-sensitive insulin secretion of cryopreserved human islets with University of Wisconsin solution and ascorbic acid-2 glucoside
    Takashi Arata
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Artif Organs 28:529-36. 2004
    ..Human islets monolayer-cultured with 804G-derived ECM are efficiently transduced with a lentiviral vector Lt-NLS/LacZ...
  27. ncbi request reprint Lentiviral transfer of the LacZ gene into human endothelial cells and human bone marrow mesenchymal stem cells
    Toshinori Totsugawa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan
    Cell Transplant 11:481-8. 2002
    ..These findings provide evidence that lentiviral vectors are efficient tools for gene transfer and expression in human endothelial cells and stem cells that could be useful for tissue engineering...
  28. ncbi request reprint Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers
    Takamasa Watanabe
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 75:1873-80. 2003
    ..Because the limited lifespan of human HSC is a major hurdle to their use, the authors report here the amplification of human HSC populations in vitro by retroviral transfer of human telomerase reverse transcriptase (hTERT)...
  29. ncbi request reprint Lentiviral vector: a useful tool for transduction of human liver endothelial cells
    Toshinori Totsugawa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan
    ASAIO J 49:635-40. 2003
    ..These findings provide evidence that lentivirus based gene delivery is an efficient tool for transduction of endothelial cells that could be considered for cell and gene therapies and hybrid artificial organs...
  30. ncbi request reprint Establishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT
    Masanobu Maruyama
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 77:446-51. 2004
    ..Here, we describe the immortalization of human cholangiocytes using serial transfection of simian virus 40 large T (SV40T) followed by human telomerase reverse transcriptase (hTERT)...
  31. ncbi request reprint Transplantation of human hepatocytes cultured with deleted variant of hepatocyte growth factor prolongs the survival of mice with acute liver failure
    Yong Chen
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 79:1378-85. 2005
    ..As a tool for maintaining hepatocyte functions in vitro, dHGF, a variant of HGF (hepatocyte growth factor) with a deletion of five amino acids, attracted our attention because it is less cytotoxic compared with HGF...
  32. ncbi request reprint Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts
    Atsushi Miki
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cell Transplant 15:325-34. 2006
    ..A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system...
  33. ncbi request reprint Reestablishment of microenvironment is necessary to maintain in vitro and in vivo human islet function
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 17:111-9. 2008
    ..These studies indicate that the reestablishment of the cell-matrix interactions by a synthetic matrix in the immediate postisolation period is a useful tool to maintain islet functions in vitro and in vivo...
  34. ncbi request reprint Construction and transplantation of an engineered hepatic tissue using a polyaminourethane-coated nonwoven polytetrafluoroethylene fabric
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 83:129-37. 2007
    ..Here, we evaluate the construction and transplantation of an engineered hepatic tissue (EHT) using primary isolated hepatocytes cultured onto polyaminourethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric...
  35. doi request reprint Hepatic differentiation of mouse iPS cells in vitro
    Masaya Iwamuro
    Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:841-7. 2010
    ..Our study will be an important step to generate hepatocytes from human iPS cells as a new source for liver-targeted cell therapies...
  36. ncbi request reprint Maintenance of cold-preserved porcine hepatocyte function with UW solution and ascorbic acid-2 glucoside
    Michihiko Takesue
    Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki 701 0192, Japan
    Cell Transplant 12:599-606. 2003
    ..The present work demonstrates that a combination of UW solution with AA2G (100 microg/ml) would be a useful cold preservation means for the development of cell therapies...
  37. ncbi request reprint Establishment of an immortalized human hepatic stellate cell line to develop antifibrotic therapies
    Norikuni Shibata
    Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki 701 0114, Japan
    Cell Transplant 12:499-507. 2003
    ..These findings suggested that immortalized activated HSC line TWNT-4 would be a useful means to develop antifibrotic therapies...
  38. ncbi request reprint Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:921-7. 2006
    ..The present work clearly demonstrates the usefulness of SAPNF for maintaining differentiated functions of porcine hepatocytes in culture...
  39. pmc Isolation and propagation of a human CD133(-) colon tumor-derived cell line with tumorigenic and angiogenic properties
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:865-77. 2010
    ..In addition, NANK secreted high amounts of important angiogenic cytokines. These results provide a novel and extensive model in human CSC for studying the generation and maintenance of phenotypic heterogeneity in CRC...
  40. ncbi request reprint Differentiation of human embryonic stem cells to hepatocytes using deleted variant of HGF and poly-amino-urethane-coated nonwoven polytetrafluoroethylene fabric
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cell Transplant 15:335-41. 2006
    ..In the present work we have demonstrated the efficient differentiation of hES cells to functional hepatocytes. The findings are useful to develop a BAL...
  41. ncbi request reprint Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells
    Keisuke Nishioka
    Division of Hematology Oncology, Department of Medicine, Hyogo College of Medicine, Hyogo 663 8501, Japan
    Int J Oncol 23:925-32. 2003
    ..This cell line may be of value for developing strategies for ex vivo expansion of human HPC...
  42. ncbi request reprint Lentivirus-based gene delivery in mouse embryonic stem cells
    Yoshikazu Kosaka
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Artif Organs 28:271-7. 2004
    ..HIV-1-derived lentiviral vectors are now considered to be an efficient vehicle for delivering genes into a variety of cells. In this study, we examined the efficacy of lentivirus-based gene delivery into mouse ES (mES) cells...
  43. ncbi request reprint Cell-permeable pentapeptide V5 inhibits apoptosis and enhances insulin secretion, allowing experimental single-donor islet transplantation in mice
    Jorge D Rivas-Carrillo
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho, Okayama, Japan
    Diabetes 56:1259-67. 2007
    ..Following isolation and transplantation, islets are susceptible to apoptosis, which limits their function and probably long-term islet graft survival...
  44. ncbi request reprint Maintenance of neovascularization at the implantation site of an artificial device by bFGF and endothelial cell transplant
    Atsushi Miki
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:893-901. 2006
    ..We believe that the present study will contribute to BAP development for the treatment of diabetes...
  45. ncbi request reprint Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2
    Jorge David Rivas-Carrillo
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:939-44. 2006
    ..In contrast, the transplantation of islets without FGF-2 supplementation showed poor revascularization and failed to control the blood glucose levels in the diabetic mice...
  46. ncbi request reprint Instant hepatic differentiation of human embryonic stem cells using activin A and a deleted variant of HGF
    Yong Chen
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:865-71. 2006
    ..We developed an efficient method to differentiate KhES-1 cells into hepatocyte-like cells in vitro using 50 ng/ml of activin A and 500 ng/ml of dHGF...
  47. ncbi request reprint Postnatal neovascularization by endothelial progenitor cells immortalized with the simian virus 40T antigen gene
    Hui Ying Qiu
    Division of Hematology and Oncology, Department of Medicine, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya, Hyogo 663 8501, Japan
    Int J Oncol 28:815-21. 2006
    ..The immortalized HYCEC-1 cells are suggested to be a class of EPCs that can efficiently participate in postnatal neovasculogenesis in the ischemic hind limb, and may also be a useful tool for studying tumor vessel formation...
  48. ncbi request reprint Development of a porcine model of type 1 diabetes by total pancreatectomy and establishment of a glucose tolerance evaluation method
    Kazuya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho, Okayama, Japan
    Artif Organs 28:1035-42. 2004
    ..This article reports the surgical technique of total pancreatectomy in pigs and the biochemical analysis of the characteristics of totally pancreatectomized pigs...
  49. ncbi request reprint A newly developed bioartificial pancreas successfully controls blood glucose in totally pancreatectomized diabetic pigs
    Hideaki Ikeda
    Department of Surgery, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan
    Tissue Eng 12:1799-809. 2006
    ..These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans...
  50. pmc Treatment of acute liver failure in mice by hepatocyte xenotransplantation
    Tsuyoshi Yamamoto
    Department of Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:799-806. 2010
    ..Hepatocytes culture in a truly 3D network allows in vivo maintaining of differentiated functions, and once transplanted between widely divergent species can function to correct acute liver failure in mice and prolong their survival...
  51. ncbi request reprint Adenovirus mediated gene transduction of primarily isolated mouse islets
    Michiki Narushima
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    ASAIO J 50:586-90. 2004
    ..To achieve an efficient adenoviral gene transfer to the pancreatic beta-cells, monolayer formation of the islets is critical. Such culture conditions were facilitated by combining 804G-ECM with IBMX...
  52. ncbi request reprint Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination
    Toshinori Totsugawa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    J Hepatol 47:74-82. 2007
    ..Hepatocyte transplantation and bioartificial liver treatment are attractive alternatives to liver transplantation. The availability of well-characterized human hepatocyte lines facilitates such cell therapies...
  53. ncbi request reprint Neovascularization induced around an artificial device implanted in the abdomen by the use of gelatinized fibroblast growth factor 2
    Takeshi Yuasa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 18:683-8. 2009
    ..There were only minor instances of fibrosis and inflammation reaction around the BAP, thus indicating the BAP that we are currently developing to have an excellent histocompatibility...
  54. doi request reprint Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice
    Hiroyuki Nakahara
    Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Transplantation 88:346-53. 2009
    ..The hMSCs may provide a potential therapy to restore damaged tissues or organs of mesenchymal origin; however, a drawback is their limited life span in vitro...
  55. ncbi request reprint Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Biotechnol 24:1412-9. 2006
    ..After functioning in the BAL, ES cell-derived hepatocytes developed characteristics nearly identical to those of primary hepatocytes...
  56. ncbi request reprint Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Protoc 2:347-56. 2007
    ..The sorted cells produce albumin in culture and metabolize ammonia, lidocaine and diazepam at approximately two-thirds the rate of primary mouse hepatocytes...
  57. doi request reprint Characteristics of CD133(+) human colon cancer SW620 cells
    Hironobu Kawamoto
    Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:857-64. 2010
    ..In conclusion, the human colon cancer cell line, SW620, contains both CD133(+) and CD133(-) phenotypes, and the CD133(+) phenotype has characteristics consistent with those of cancer stem cells...
  58. ncbi request reprint Laparoscopy-assisted creation of a liver failure model in pigs
    Takeshi Yuasa
    Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 17:187-93. 2008
    ..We believe that our model is the first report on creation of a reliable model for liver failure in pigs to assess the efficacy of liver-targeted cell therapies...
  59. ncbi request reprint A human beta-cell line for transplantation therapy to control type 1 diabetes
    Michiki Narushima
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Biotechnol 23:1274-82. 2005
    ..The establishment of this cell line is one step toward a potential cure of diabetes by transplantation...
  60. doi request reprint Transplantation of osteogenically differentiated mouse iPS cells for bone repair
    Takahiro Hayashi
    Department of Orthopeadic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 21:591-600. 2012
    ..It is believed that irradiation before transplantation is an effective way to inhibit teratoma formation...
  61. ncbi request reprint PuraMatrix facilitates bone regeneration in bone defects of calvaria in mice
    Haruo Misawa
    Department of Orthopeadic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Cell Transplant 15:903-10. 2006
    ..The strength of the regenerated bone was 1.72-fold higher for PuraMatrix (146.0 g) than for Matrigel (84.7 g). The present study demonstrated that PuraMatrix injection favorably induced functional bone regeneration...
  62. ncbi request reprint A new cell-permeable peptide allows successful allogeneic islet transplantation in mice
    Hirofumi Noguchi
    Department of Physiology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama, 700 8558 Japan
    Nat Med 10:305-9. 2004
    ..In addition, it did not affect insulin secretion, whereas FK506 caused a dose-dependent decrease in insulin secretion. Cell-permeable peptides can thus provide a new strategy for drug development and may eventually be useful clinically...
  63. ncbi request reprint Transduction of immortalized human hepatocytes with p21 to enhance differentiated phenotypes
    Takemi Kunieda
    Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan
    Cell Transplant 11:421-8. 2002
    ..We here show that exogenous expression of p21 augments cellular differentiation in immortalized human NKNT-3 cells...
  64. ncbi request reprint Hepatocyte isolation and transplantation in the pig
    Masanobu Maruyama
    Department of Surgery, Okayama University Medical School, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cell Transplant 12:593-8. 2003
    ..We describe an efficient porcine hepatocyte isolation and subsequent cell transplantation in pigs with D-gal-induced liver failure...
  65. ncbi request reprint Long-term culture of Japanese human embryonic stem cells in feeder-free conditions
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 17:27-33. 2008
    ..Here we demonstrated that Japanese human embryonic stem cells KhES-1 were cultured long term in a feeder-free method, while retaining pluripotency in vitro...
  66. ncbi request reprint Construction of a differentiated human hepatocyte cell line expressing the herpes simplex virus-thymidine kinase gene
    N Kobayashi
    First Department of Surgery, Okayama University Medical School, Japan
    ASAIO J 47:476-80. 2001
    ..In the present study, we have established highly differentiated immortalized human hepatocytes with tight regulation. The cells may be clinically useful for HAL treatment...
  67. ncbi request reprint Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4alpha2 gene
    Y Inoue
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2 5 1 Shikata, Okayama 700 8558, Japan
    Int J Mol Med 8:481-7. 2001
    ..Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions...
  68. ncbi request reprint Vascular endothelial growth factor secreted by replicating hepatocytes induces sinusoidal endothelial cell proliferation during regeneration after partial hepatectomy in rats
    H Shimizu
    First Department of Surgery, School of Medicine, Chiba University, Japan
    J Hepatol 34:683-9. 2001
    ..CONCLUSIONS: These data strongly suggest that VEGF secreted by proliferating hepatocytes may represent an important stimulator of SEC proliferation...
  69. pmc Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700 8558, Japan
    J Cell Biol 149:1193-206. 2000
    ..These data indicate the possible involvement of nuclear S100C in the contact inhibition of cell growth...
  70. ncbi request reprint Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes
    N Kobayashi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Japan
    Cell Transplant 9:733-5. 2000
    ..Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment...
  71. ncbi request reprint Synergism between interferon-gamma and cAMP in induction of hepatocyte growth factor in human skin fibroblasts
    E Gohda
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima Naka, Okayama, 700 8530, Japan
    Cytokine 12:780-5. 2000
    ..These results indicate that IFN-gamma synergistically stimulates cAMP-induced HGF production and inhibits HGF production induced by growth factors and protein kinase C activators in human skin fibroblasts...
  72. ncbi request reprint Increased hepatocyte growth factor production by aging human fibroblasts mainly due to autocrine stimulation by interleukin-1
    M Miyazaki
    Department of Cell Biology, Okayama University Medical School, Japan
    Biochem Biophys Res Commun 246:255-60. 1998
    ..The increased production of HGF by aging fibroblasts from human embryonic lung tissue is mainly due to autocrine stimulation by interleukin-1...
  73. ncbi request reprint A REIC gene shows down-regulation in human immortalized cells and human tumor-derived cell lines
    T Tsuji
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Shikata cho 2 5 1, Okayama, 700 8558, Japan
    Biochem Biophys Res Commun 268:20-4. 2000
    ..Furthermore, a search of the protein database revealed a match of this gene product with Dkk-3, which is a novel inhibitor of Wnt oncogene. These results indicate that the REIC cloned by us may function as a tumor suppressor...
  74. ncbi request reprint Transforming growth factor-beta 1 stimulates or inhibits cell growth via down- or up-regulation of p21/Waf1
    M Miyazaki
    Department of Cell Biology, Okayama University Medical School, Japan
    Biochem Biophys Res Commun 246:873-80. 1998
    ..Thus the cell growth stimulation or inhibition by TGF-beta 1 are mediated by the down- or up-regulation of p21/ Waf1, respectively...
  75. ncbi request reprint Inhibitory effects of antioxidants on neonatal rat cardiac myocyte hypertrophy induced by tumor necrosis factor-alpha and angiotensin II
    K Nakamura
    Department of Cardiovascular Medicine, Okayama University Medical School, Japan
    Circulation 98:794-9. 1998
    ..Signal transduction pathways of those factors are implicated in reactive oxygen intermediates (ROIs). Therefore, we hypothesized that TNF-alpha and Ang II might cause myocyte hypertrophy via the generation of ROIs...
  76. ncbi request reprint A new human chondrosarcoma cell line (OUMS-27) that maintains chondrocytic differentiation
    T Kunisada
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Shikata, Japan
    Int J Cancer 77:854-9. 1998
    ..Moreover, OUMS-27 cells had p53-gene mutation. Thus, the OUMS-27 cell line can provide a useful model not only for studies on human chondrocyte but also for basic studies on the diagnosis, treatment and etiology of human chondrosarcoma...
  77. ncbi request reprint Up-regulation of S100C in normal human fibroblasts in the process of aging in vitro
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700-8558, Japan
    Exp Gerontol 36:1317-25. 2001
    ..These findings suggest that an increase in the cells having nuclear accumulation of S100C is closely related to the process of cellular senescence of normal human fibroblasts...
  78. ncbi request reprint Stem cell research and therapy for liver disease
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho, Japan
    Curr Stem Cell Res Ther 4:141-6. 2009
    ..This review describes the current challenges and future opportunities in Embryonic Stem cell differentiation to liver cells, and the appropriate characteristics needed for their future clinical use in the treatment of liver disease...
  79. ncbi request reprint Current cell-based approaches for the treatment of diabetes mellitus
    Jorge David Rivas-Carrillo
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Biotechnol Genet Eng Rev 24:281-95. 2007
  80. ncbi request reprint Portal vein infusion of cancer chemotherapeutic agent emulsified with Lipiodol in regenerating liver after partial hepatectomy in rats
    M Miyazaki
    First Department of Surgery, School of Medicine, Chiba University, Japan
    Res Exp Med (Berl) 193:231-40. 1993
    ..This study demonstrates that in rats LP has a powerful effect on the long-term retention of anticancer agents in the sinusoidal space after infusion into the portal vein, without aggravating hepatic damage by anticancer agents...
  81. ncbi request reprint Transplantation of reversibly immortalized insulin-secreting human hepatocytes controls diabetes in pancreatectomized pigs
    Teru Okitsu
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Diabetes 53:105-12. 2004
    ..We suggest that this reversibly immortalized, insulin-secreting human hepatocyte line may overcome the shortage of donor pancreata for islet transplantation into patients with type 1 diabetes...
  82. ncbi request reprint [Liver-targeted regenerative medicine]
    Naoya Kobayashi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Nihon Geka Gakkai Zasshi 105:440-4. 2004
    ..This reversible immortalization system allows us to establish a highly safe human hepatocyte line that is capable of differentiation at a lower cost and on a larger scale...
  83. ncbi request reprint Establishment and cellular characteristics of a hepatocyte cell line (OUMS-31) derived from an acatalasemic mouse
    A Kondo
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Japan
    In Vitro Cell Dev Biol Anim 35:155-8. 1999
    ..This hepatocyte cell line should be useful for studying effects of oxidative radical stress at the cellular level...
  84. ncbi request reprint Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts
    C Gao
    Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, 2-5-1 Shikata, Okayama 700-8558, Japan
    Int J Oncol 18:871-5. 2001
    ..These results suggest that the overexpression of PDGF-B (c-sis) and downregulation of PDGFR-alpha are related to the phenotypic characteristics of immortalized human cells...
  85. ncbi request reprint Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas
    T Tsuji
    Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry, Shikata cho 2 5 1, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 289:257-63. 2001
    ..We determined the REIC/Dkk-3 locus on chromosome 11p15, where loss of heterozygosity has frequently been observed in human tumors. These findings indicate that REIC/Dkk-3 may function as a tumor suppressor...
  86. ncbi request reprint Secretion of extracellular matrix (fibronectin), growth factor (transforming growth factor beta) and protease (cathepsin D) by hepatoma cells
    H Ito
    First Department of Surgery, School of Medicine, Chiba University, Japan
    Oncology 58:261-70. 2000
    ..These actions indicate that hepatocellular carcinoma may possess a forceful autocrine mechanism enabling cells to survive and proliferate under cirrhotic conditions...
  87. ncbi request reprint Identification of a phosphoprotein that is downregulated in immortalized human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama, Japan
    Electrophoresis 22:155-60. 2001
    ..These results suggest that an abnormal regulation of HSP27 expression and phosphorylation may be one of the reasons for easy neoplastic transformation of the immortalized cells by the treatment with carcinogenic agents...
  88. ncbi request reprint Effects of barbiturates with or without liver-tumor-promoting activity on survival and DNA synthesis of suckling and adult rat hepatocytes in serum-free primary culture
    M Miyazaki
    Department of Cell Biology, Okayama University Medical School, Japan
    J Cancer Res Clin Oncol 118:435-40. 1992
    ....
  89. ncbi request reprint Establishment of a human hepatocyte line (OUMS-29) having CYP 1A1 and 1A2 activities from fetal liver tissue by transfection of SV40 LT
    K Fukaya
    Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan
    In Vitro Cell Dev Biol Anim 37:266-9. 2001
    ..This cell line is expected to be instrumental as an alternative method in animal experiments for studying hepatocarcinogenesis, drug metabolisms of liver cells, and hepatic toxicology...
  90. ncbi request reprint Immunosuppression for islet transplantation
    Hirofumi Noguchi
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Acta Med Okayama 60:71-6. 2006
    ..In this review, we show some of the most recent contributions to the advancement of knowledge in this field...
  91. ncbi request reprint Functional hepatocyte culture and its application to cell therapies
    Kimiaki Tanaka
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:855-64. 2006
    ..We also introduce a new type of liver tissue engineering, hepatocyte sheet transplantation, which utilizes unwoven cloth having a cellular adhesive property...
  92. doi request reprint Intramuscular transplantation of engineered hepatic tissue constructs corrects acute and chronic liver failure in mice
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho, Okayama, Japan
    J Hepatol 52:211-9. 2010
    ..Strategies that allow repeated extrahepatic infusion of hepatocytes could improve the efficacy and safety of hepatocyte transplantation for the treatment of liver failure...
  93. ncbi request reprint Increased cytotoxicity of carbon tetrachloride in a human hepatoma cell line overexpressing cytochrome P450 2E1
    S Takahashi
    Department of Anaesthesiology and Resuscitology, Okayama University Medical School, Okayama City, Japan
    J Int Med Res 30:400-5. 2002
    ..These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells...
  94. ncbi request reprint In vivo and in vitro test for growth potential of liver cells from rats during early stage of hepatocarcinogenesis by 3'-methyl-4-dimethylaminoazobenzene
    M Miyazaki
    Division of Pathology, Cancer Institute, Okayama University Medical School, Japan
    J Cancer Res Clin Oncol 115:1-8. 1989
    ..As described above, liver cells from rats fed 3'-Me-DAB demonstrated much greater proliferative ability than normal control cells in vivo and in vitro...
  95. ncbi request reprint Left-hand-assisted laparoscopic resection of hepatocellular carcinoma in an accessory liver
    K Iwase
    Department of Surgery, Rinku General Medical Center, Izumisano Municipal Hospital, 2-23, Rinku Orai-Kita, Izumisano, Osaka 598-8577, Japan
    J Hepatobiliary Pancreat Surg 8:379-82. 2001
    ..Left-hand-assisted laparoscopic procedures would be feasible for the easy and safe resection of localized hepatocellular carcinoma developing in an accessory liver...
  96. ncbi request reprint Influence of liver tumor promoters and structurally related chemicals on survival of normal adult rat hepatocytes in primary culture
    M Miyazaki
    Division of Pathology, Okayama University Medical School, Japan
    J Cancer Res Clin Oncol 116:259-63. 1990
    ....
  97. ncbi request reprint Mechanism of cold ischemia-reperfusion-induced graft injury after orthotopic liver transplantation in rats
    H Shimizu
    First Department of Surgery, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan
    Hepatogastroenterology 48:216-9. 2001
    ..This phenomenon may subsequently cause ischemic damage to the hepatocyte, with eventual graft failure...
  98. ncbi request reprint Loss of nuclear localization of the S100C protein in immortalized human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Okayama 700-8558, Japan
    Radiat Res 155:208-214. 2001
    ..Taken together, loss of nuclear localization of the S100C protein, which may be related to DNA synthesis, is thought to be one of the mechanisms of immortalization...
  99. ncbi request reprint Enhanced expression of cyclin E and cyclin A in human hepatocellular carcinomas
    R Ohashi
    Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, 2-5-1 Shikata, Okayama 700-8558, Japan
    Anticancer Res 21:657-62. 2001
    ..CONCLUSIONS: The degree of cyclin E expression can be used as a prognostic parameter of HCC. In addition, cyclin E may become a molecular target in the treatment of HCCs...
  100. ncbi request reprint Immortalized hepatocytes using human artificial chromosome
    Masahiro Ito
    Department of Surgery, Fujita Health University, Toyaoke City, Aichi, Japan
    Cell Transplant 17:165-71. 2008
    ..In further experiments, this cell line will be used for transplantable cells or artificial organ using HAC. These results represent an important step toward the development of immortalized hepatocytes...
  101. doi request reprint Hepatic stem cells and liver development
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Methods Mol Biol 640:181-236. 2010
    ..Although a human stem cell-derived cell type equivalent to primary hepatocytes does not yet exist, the promising results obtained with extrahepatic stem cells would open the way to cell-based therapy for liver diseases...