Nobuyuki Kato

Summary

Affiliation: Okayama University
Country: Japan

Publications

  1. pmc Genetic characterization of hepatitis C virus in long-term RNA replication using Li23 cell culture systems
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Shikata cho, Okayama, Japan
    PLoS ONE 9:e91156. 2014
  2. ncbi request reprint Establishment of a hepatitis C virus subgenomic replicon derived from human hepatocytes infected in vitro
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
    Biochem Biophys Res Commun 306:756-66. 2003
  3. ncbi request reprint [Replication of hepatitis C virus genome]
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
    Uirusu 58:191-8. 2008
  4. doi request reprint Genetic variability and diversity of intracellular genome-length hepatitis C virus RNA in long-term cell culture
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University of Graduate School of Medicine, Okayama, Japan
    Arch Virol 154:77-85. 2009
  5. ncbi request reprint [Hepatitis virus--the approach to clarification of the mechanisms of virus proliferation and the development of its control means]
    Nobuyuki Kato
    Okayama University Graduate School of Medicine and Dentistry, Department of Molecular Biology, Shikata cho, 2 5 1, Okayama 700 8558
    Uirusu 52:157-62. 2002
  6. ncbi request reprint Genetic variation and dynamics of hepatitis C virus replicons in long-term cell culture
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    J Gen Virol 86:645-56. 2005
  7. ncbi request reprint Molecular virology of hepatitis C virus
    N Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Acta Med Okayama 55:133-59. 2001
  8. doi request reprint Anti-ulcer agent teprenone inhibits hepatitis C virus replication: potential treatment for hepatitis C
    Masanori Ikeda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Liver Int 31:871-80. 2011
  9. ncbi request reprint Establishment of hepatitis C virus replicon cell lines possessing interferon-resistant phenotype
    Katsuyuki Namba
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 323:299-309. 2004
  10. ncbi request reprint A new living cell-based assay system for monitoring genome-length hepatitis C virus RNA replication
    Hiromichi Dansako
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Virus Res 137:72-9. 2008

Collaborators

Detail Information

Publications73

  1. pmc Genetic characterization of hepatitis C virus in long-term RNA replication using Li23 cell culture systems
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Shikata cho, Okayama, Japan
    PLoS ONE 9:e91156. 2014
    ..On the other hand, we newly established HCV RNA-replicating cell lines using human hepatoma Li23 cells, which were distinct from HuH-7 cells...
  2. ncbi request reprint Establishment of a hepatitis C virus subgenomic replicon derived from human hepatocytes infected in vitro
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, 700 8558, Okayama, Japan
    Biochem Biophys Res Commun 306:756-66. 2003
    ..Taken together, present results indicate that the replicon RNA library generated using an in vitro HCV infection system is useful for the establishment of an HCV subgenomic replicon...
  3. ncbi request reprint [Replication of hepatitis C virus genome]
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
    Uirusu 58:191-8. 2008
    ....
  4. doi request reprint Genetic variability and diversity of intracellular genome-length hepatitis C virus RNA in long-term cell culture
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University of Graduate School of Medicine, Okayama, Japan
    Arch Virol 154:77-85. 2009
    ..These results suggest that an HCV-RNA-replicating cell culture system would be useful for analysis of the evolutionary dynamics and variations of HCV...
  5. ncbi request reprint [Hepatitis virus--the approach to clarification of the mechanisms of virus proliferation and the development of its control means]
    Nobuyuki Kato
    Okayama University Graduate School of Medicine and Dentistry, Department of Molecular Biology, Shikata cho, 2 5 1, Okayama 700 8558
    Uirusu 52:157-62. 2002
  6. ncbi request reprint Genetic variation and dynamics of hepatitis C virus replicons in long-term cell culture
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    J Gen Virol 86:645-56. 2005
    ..Using this replicon cell culture system, it was demonstrated further that neither ribavirin nor its derivative mizoribine accelerated the mutation rate or the increase in the genetic diversity of HCV replicon...
  7. ncbi request reprint Molecular virology of hepatitis C virus
    N Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Acta Med Okayama 55:133-59. 2001
    ..The enormous body of information collected thus far in the field of HCV research is summarized below, and an overview of the current status of HCV molecular virology of HCV is provided...
  8. doi request reprint Anti-ulcer agent teprenone inhibits hepatitis C virus replication: potential treatment for hepatitis C
    Masanori Ikeda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Liver Int 31:871-80. 2011
    ..Geranylgeranyl pyrophosphate (GGPP) is a substrate for geranylgeranyltransferase. Therefore, we examined the potential of geranyl compounds with chemical structures similar to those of GGPP to inhibit HCV RNA replication...
  9. ncbi request reprint Establishment of hepatitis C virus replicon cell lines possessing interferon-resistant phenotype
    Katsuyuki Namba
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 323:299-309. 2004
    ..These newly established HCV replicon cell lines possessing IFN-resistant phenotypes are the first useful tools for understanding the mechanisms by which HCV acquires IFN resistance in vivo...
  10. ncbi request reprint A new living cell-based assay system for monitoring genome-length hepatitis C virus RNA replication
    Hiromichi Dansako
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Virus Res 137:72-9. 2008
    ..The OGF7 assay system is expected to be the most time-saving and inexpensive assay system for high-throughput screening of anti-HCV reagents...
  11. doi request reprint HCV genotype 1b chimeric replicon with NS5B of JFH-1 exhibited resistance to cyclosporine A
    Ken ichi Abe
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikata cho, Okayama, Japan
    Arch Virol 154:1671-7. 2009
    ..Here, we demonstrated that NS5B of JFH-1 contributed to this strain's CsA-resistant phenotype. NS5B and CyPA are significant for determining HCV's sensitivity to CsA...
  12. ncbi request reprint Epigenetic silencing of interferon-inducible genes is implicated in interferon resistance of hepatitis C virus replicon-harboring cells
    Kazuhito Naka
    Department of Molecular Biology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    J Hepatol 44:869-78. 2006
    ..Here, we aimed to clarify the mechanism(s) underlying the partial IFN resistance of the alphaR-series cells...
  13. ncbi request reprint Efficient replication of a full-length hepatitis C virus genome, strain O, in cell culture, and development of a luciferase reporter system
    Masanori Ikeda
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Biochem Biophys Res Commun 329:1350-9. 2005
    ..This culture system is useful tool for the study of HCV replication and mass screening for anti-HCV reagents...
  14. ncbi request reprint Tandem repeats of lactoferrin-derived anti-hepatitis C virus peptide enhance antiviral activity in cultured human hepatocytes
    Ken ichi Abe
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Japan
    Microbiol Immunol 51:117-25. 2007
    ..These results suggest that tandem repeats of LF-derived anti-HCV peptide are useful as anti-HCV reagents...
  15. pmc The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication
    Yasuo Ariumi
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    J Virol 82:9639-46. 2008
    ..Taken together, these results suggest that the ATM signaling pathway is critical for HCV RNA replication and may represent a novel target for the clinical treatment of patients with chronic hepatitis C...
  16. doi request reprint Amino acid substitutions of hepatitis C virus core protein are not associated with intracellular antiviral response to interferon-α in vitro
    Fusao Ikeda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Liver Int 30:1324-31. 2010
    ....
  17. doi request reprint New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C
    Kyoko Mori
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 371:104-9. 2008
    ..Reporter assay analysis suggests that the diverse effects of interferon-gamma are due to the difference in HCV strains, but not the cellular environment...
  18. pmc New preclinical antimalarial drugs potently inhibit hepatitis C virus genotype 1b RNA replication
    Youki Ueda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Shikata cho, Okayama, Japan
    PLoS ONE 8:e72519. 2013
    ..Thus a new type of anti-HCV drug is still needed...
  19. ncbi request reprint Different anti-HCV profiles of statins and their potential for combination therapy with interferon
    Masanori Ikeda
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Hepatology 44:117-25. 2006
    ..In combination with IFN, fluvastatin also exhibited a synergistic inhibitory effect. In conclusion, statins, especially fluvastatin, could be potentially useful as new anti-HCV reagents in combination with IFN...
  20. doi request reprint Replicons from genotype 1b HCV-positive sera exhibit diverse sensitivities to anti-HCV reagents
    Go Nishimura
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Antiviral Res 82:42-50. 2009
    ..44 IU/ml vs. 1.37 IU/ml) and cyclosporine A (EC(50): 0.71 microg/ml vs. 0.96 microg/ml). These replicons are thus useful tools for examining the mechanism of anti-HCV activity, especially in IFN-lambda and statins...
  21. ncbi request reprint Limited suppression of the interferon-beta production by hepatitis C virus serine protease in cultured human hepatocytes
    Hiromichi Dansako
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Japan
    FEBS J 274:4161-76. 2007
    ..Taken together, our findings suggest that the blocking of the interferon production by NS3-4A is not sufficient in HCV-infected hepatocyte cells...
  22. ncbi request reprint cDNA microarray analysis of lactoferrin expression in non-neoplastic human hepatocyte PH5CH8 cells
    Takahiko Tamura
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochim Biophys Acta 1721:73-80. 2005
    ..This modulation is one of the cellular responses that the GABA-B receptor modifies. This is the first report of microarray analysis applied to search inclusively for the target genes of LF...
  23. doi request reprint Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system
    Kyoko Mori
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Virus Res 157:61-70. 2011
    ..In conclusion, we demonstrated for the first time that inhibition of IMPDH is a major antiviral target by which RBV at clinically achievable concentrations inhibits HCV RNA replication...
  24. ncbi request reprint Promotion of microsatellite instability by hepatitis C virus core protein in human non-neoplastic hepatocyte cells
    Atsushi Naganuma
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cancer Res 64:1307-14. 2004
    ..This is the first report to demonstrate that the core protein may disturb the DNA repair system...
  25. ncbi request reprint Adenosine kinase is a key determinant for the anti-HCV activity of ribavirin
    Kyoko Mori
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Hepatology 58:1236-44. 2013
    ..Finally, we demonstrated that the long 5' UTR functioned as an IRES in primary human hepatocytes...
  26. doi request reprint Identification of host genes showing differential expression profiles with cell-based long-term replication of hepatitis C virus RNA
    Hiroe Sejima
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    Virus Res 167:74-85. 2012
    ..5-year replication of HCV RNA. These genes may play roles in the optimization of the environment in HCV RNA replication, or may play key roles in the progression of HCV-associated hepatic diseases...
  27. doi request reprint Oncostatin M synergistically inhibits HCV RNA replication in combination with interferon-alpha
    Masanori Ikeda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    FEBS Lett 583:1434-8. 2009
    ..OSM is a candidate anti-HCV reagent and may improve the current IFN therapy for patients with chronic hepatitis C...
  28. ncbi request reprint cDNA microarray analysis to compare HCV subgenomic replicon cells with their cured cells
    Ken ichi Abe
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Virus Res 107:73-81. 2005
    ..Our microarray analysis demonstrated that HCV subgenomic replicons can change the gene expression profiles of host cells, and it allowed us to compile the first list of genes that the replicons transcriptionally regulate...
  29. ncbi request reprint Oxidative stress induces anti-hepatitis C virus status via the activation of extracellular signal-regulated kinase
    Masahiko Yano
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Hepatology 50:678-88. 2009
    ..We propose that a cellular process that follows ERK1/2 phosphorylation and is specific to oxidative stimulation might lead to down-regulation of HCV RNA replication...
  30. ncbi request reprint Efficient replication systems for hepatitis C virus using a new human hepatoma cell line
    Nobuyuki Kato
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Virus Res 146:41-50. 2009
    ..Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets...
  31. ncbi request reprint Mizoribine inhibits hepatitis C virus RNA replication: effect of combination with interferon-alpha
    Kazuhito Naka
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 330:871-9. 2005
    ..Since mizoribine is currently used in several clinical applications and has not been associated with severe side effects, mizoribine is considered to be of potential use as a new anti-HCV reagent in combination with IFN-alpha...
  32. pmc DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication
    Yasuo Ariumi
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    J Virol 81:13922-6. 2007
    ..Thus, DDX3 is required for HCV RNA replication...
  33. ncbi request reprint Cell culture-adaptive NS3 mutations required for the robust replication of genome-length hepatitis C virus RNA
    Ken ichi Abe
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Virus Res 125:88-97. 2007
    ..These findings suggest that specific viral factors may affect the replication level of genome-length HCV RNA...
  34. doi request reprint PML tumor suppressor protein is required for HCV production
    Misao Kuroki
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 430:592-7. 2013
    ..Finally, we also demonstrated that INI1 and DDX5, the PML-related proteins, are involved in HCV production. Taken together, these findings suggest that PML is required for HCV production...
  35. doi request reprint Double-stranded RNA-induced interferon-beta and inflammatory cytokine production modulated by hepatitis C virus serine proteases derived from patients with hepatic diseases
    Hiromichi Dansako
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Arch Virol 154:801-10. 2009
    ..These results suggest that the inhibitory effects of NS3-4A on antiviral signaling pathways are limited to the Cardif-mediated pathway in human hepatocytes...
  36. ncbi request reprint Interferon resistance of hepatitis C virus replicon-harbouring cells is caused by functional disruption of type I interferon receptors
    Kazuhito Naka
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    J Gen Virol 86:2787-92. 2005
    ..Moreover, it was shown that ectopic expression of wild-type IFNAR1 in IFN-resistant phenotype, replicon-harbouring cells possessing the IFNAR1 mutant restored type I IFN signalling...
  37. ncbi request reprint Anti-HCV activity of the Chinese medicinal fungus Cordyceps militaris
    Youki Ueda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 447:341-5. 2014
    ..In consideration of all these results, we suggest that CM would be useful as an oral anti-HCV agent in combination with interferon-α and/or ribavirin. ..
  38. pmc The ESCRT system is required for hepatitis C virus production
    Yasuo Ariumi
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    PLoS ONE 6:e14517. 2011
    ..Retroviruses and some other enveloped viruses require an endosomal sorting complex required for transport (ESCRT) components and their associated proteins for their budding process...
  39. doi request reprint Development of a drug assay system with hepatitis C virus genome derived from a patient with acute hepatitis C
    Kyoko Mori
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama, 700 8558, Japan
    Virus Genes 44:374-81. 2012
    ..Taken together, these results suggest that the AH1R assay system is useful for the objective evaluation of anti-HCV reagents and for the discovery of different classes of anti-HCV reagents...
  40. ncbi request reprint Differential activation of interferon-inducible genes by hepatitis C virus core protein mediated by the interferon stimulated response element
    Hiromichi Dansako
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Virus Res 97:17-30. 2003
    ....
  41. ncbi request reprint Eradication of hepatitis C virus subgenomic replicon by interferon results in aberrant retinol-related protein expression
    Kazuko Koike
    Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Acta Med Okayama 66:461-8. 2012
    ..The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression...
  42. doi request reprint Development of hepatitis C virus production reporter-assay systems using two different hepatoma cell lines
    Midori Takeda
    Department of Tumor Virology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8544, Japan
    J Gen Virol 93:1422-31. 2012
    ..In this study, we developed HCV JFH-1 reporter-assay systems using two distinct hepatoma cell lines, HuH-7 and Li23. The mutations in NS5B resulted in different effects on strains O and JFH-1 HCV RNA replication...
  43. ncbi request reprint Hepatitis C virus proteins exhibit conflicting effects on the interferon system in human hepatocyte cells
    Hiromichi Dansako
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 336:458-68. 2005
    ....
  44. ncbi request reprint Life style-related diseases of the digestive system: cell culture system for the screening of anti-hepatitis C virus (HCV) reagents: suppression of HCV replication by statins and synergistic action with interferon
    Masanori Ikeda
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    J Pharmacol Sci 105:145-50. 2007
    ..Furthermore, statins enhanced the anti-HCV effect of interferon in combination. Statins may be a promising candidate for the adjuvant in interferon therapy and may improve the efficiency of the current interferon and ribavirin therapy...
  45. pmc Arsenic trioxide inhibits hepatitis C virus RNA replication through modulation of the glutathione redox system and oxidative stress
    Misao Kuroki
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    J Virol 83:2338-48. 2009
    ..Taken together, these findings suggest that ATO inhibits the HCV RNA replication through modulation of the glutathione redox system and oxidative stress...
  46. ncbi request reprint Hepatitis C virus NS5B delays cell cycle progression by inducing interferon-beta via Toll-like receptor 3 signaling pathway without replicating viral genomes
    Kazuhito Naka
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Virology 346:348-62. 2006
    ..Our findings revealed that NS5B induced IFN-beta through the TLR3 signaling pathway in immortalized human hepatocytes even without replicating viral genomes...
  47. ncbi request reprint Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication
    Ken ichi Abe
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    Virus Res 125:162-8. 2007
    ..This cell culture system is expected to be a useful tool for vaccine development, as well as for further investigation of cellular factors that are essential for HCV RNA replication...
  48. pmc Comprehensive analysis of the effects of ordinary nutrients on hepatitis C virus RNA replication in cell culture
    Masahiko Yano
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Antimicrob Agents Chemother 51:2016-27. 2007
    ....
  49. pmc Raloxifene inhibits hepatitis C virus infection and replication
    Midori Takeda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700 8558, Japan
    FEBS Open Bio 2:279-83. 2012
    ..Raloxifene enhanced the anti-HCV activity of IFN-α. These results suggest a link between the molecular biology of osteoporosis and the HCV life cycle...
  50. ncbi request reprint Hepatitis C virus quasispecies in cancerous and noncancerous hepatic lesions: the core protein-encoding region
    Shahjalal S Alam
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Acta Med Okayama 56:141-7. 2002
    ....
  51. doi request reprint Plural assay systems derived from different cell lines and hepatitis C virus strains are required for the objective evaluation of anti-hepatitis C virus reagents
    Youki Ueda
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Biochem Biophys Res Commun 409:663-8. 2011
    ..Therefore, we conclude that plural HCV assay systems developed using different cell lines or HCV strains are required for the objective evaluation of anti-HCV reagents...
  52. ncbi request reprint Identification of a lactoferrin-derived peptide possessing binding activity to hepatitis C virus E2 envelope protein
    Akito Nozaki
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Japan
    J Biol Chem 278:10162-73. 2003
    ..These results led us to consider the development of an effective anti-HCV peptide. This is the first identification of a natural protein-derived peptide that specifically binds to HCV E2 protein and prevents HCV infection...
  53. ncbi request reprint Development of a hepatitis C virus relapse model using genome-length hepatitis C virus ribonucleic acid-harboring cells possessing the interferon-alpha-resistance phenotype
    Yoshinari Kawai
    Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Hepatol Res 39:898-909. 2009
    ..Thus anti-HCV reagents, which have a mechanism different from IFN-alpha, were shown to be useful for preventing a relapse of IFN-alpha-resistant HCV...
  54. ncbi request reprint Modulation of host metabolism as a target of new antivirals
    Masanori Ikeda
    Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
    Adv Drug Deliv Rev 59:1277-89. 2007
    ..These host molecules are also candidate targets for antivirals. Here, we describe the recent progress regarding the anti-HCV reagents targeting host metabolism...
  55. ncbi request reprint [Mode of proliferation of HCV in human lymphocytes]
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry
    Nihon Rinsho 62:101-6. 2004
  56. ncbi request reprint [Gene organization of hepatitis C virus and its sequence diversity]
    Nobuyuki Kato
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry
    Nihon Rinsho 62:26-31. 2004
  57. ncbi request reprint Genome of human hepatitis C virus (HCV): gene organization, sequence diversity, and variation
    N Kato
    Department of Molecular Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan
    Microb Comp Genomics 5:129-51. 2000
    ..The hypervariable region 1 found within the envelope E2 protein was shown to be a major site for the genetic evolution of HCV after the onset of hepatitis, and might be involved in escape from the host immunesurveillance system...
  58. ncbi request reprint Quantitative method of intracellular hepatitis C virus RNA using LightCycler PCR
    Akito Nozaki
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Acta Med Okayama 56:107-10. 2002
    ..We demonstrated that this method was useful for the evaluation of antiviral reagents using HCV-infected human cultured cells...
  59. ncbi request reprint [Recent advances of basic research and clinical application of lactoferrin as an antiviral reagent against chronic hepatitis C]
    Akito Nozaki
    Department of Molecular Biology, Okayama University Graduate School of Medicine and Dentistry
    Nihon Rinsho 60:819-29. 2002
    ..Further clinical trials showed that lactoferrin is a promising candidate for adjuvant therapy with interferon in patients with chronic hepatitis C...
  60. ncbi request reprint Primary structure and expression of peroxisomal acetylspermidine oxidase in the methylotrophic yeast Candida boidinii
    M Nishikawa
    Research Institute for Biological Sciences Okayama, Okayama, Japan
    FEBS Lett 476:150-4. 2000
    ..boidinii. To our knowledge, this is the first report on the gene coding for ASOD that can catalyze the oxidation of N-acetylated polyamine as a substrate, from any type of organism...
  61. ncbi request reprint Possible molecular mechanism of the relationship between NS5B polymorphisms and early clearance of hepatitis C virus during interferon plus ribavirin treatment
    Mitsuyasu Nakamura
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, Keio University, Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Med Virol 80:632-9. 2008
    ..Our results suggested that this mutation at least partly contributes to resistance to early viral clearance during interferon and ribavirin combination therapy...
  62. ncbi request reprint Subgenomic replicon derived from a cell line infected with the hepatitis C virus
    Hiroe Kishine
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, 53 Kawara cho Shogo in, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 293:993-9. 2002
    ..These results suggest that the HCV genome obtained from an in vitro HCV infection system with cultured cells can be used to develop a subgenomic replicon system with diverse HCV sequences...
  63. pmc Identification of novel epoxide inhibitors of hepatitis C virus replication using a high-throughput screen
    Lee F Peng
    GI Unit, Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA
    Antimicrob Agents Chemother 51:3756-9. 2007
    ..5 microM). We then synthesized an analog of these compounds with optimized activity...
  64. ncbi request reprint Differential effects of calcineurin inhibitors, tacrolimus and cyclosporin a, on interferon-induced antiviral protein in human hepatocyte cells
    Kumi Hirano
    Department of Clinical Pharmaceutics, Nagasaki University, Nagasaki, Japan
    Liver Transpl 14:292-8. 2008
    ..Moreover, the replication of HCV was activated. This phenomenon is detrimental to IFN therapy after liver transplantation, and the selection of calcineurin inhibitors may warrant further discussion depending on the transplant situation...
  65. ncbi request reprint A cell-based, high-throughput screen for small molecule regulators of hepatitis C virus replication
    Sun Suk Kim
    Gastrointestinal Unit, Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA
    Gastroenterology 132:311-20. 2007
    ..Thus, the identification of more effective and better tolerated agents is a high priority. We applied chemical biology to screen small molecules that regulate HCV...
  66. ncbi request reprint Zinc is a negative regulator of hepatitis C virus RNA replication
    Kazuhisa Yuasa
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
    Liver Int 26:1111-8. 2006
    ..However, the role of zinc in both viral replication and the expression of viral proteins remains unclear. We aimed to clarify the effect of zinc on the replication of HCV in vitro...
  67. ncbi request reprint Adenovirus-mediated gene transfer of interferon alpha inhibits hepatitis C virus replication in hepatocytes
    Koichi Suzuki
    Genetics Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Biochem Biophys Res Commun 307:814-9. 2003
    ..This study supports the development of IFN-alpha gene therapy for HCV-associated liver diseases...
  68. pmc Diverse effects of cyclosporine on hepatitis C virus strain replication
    Naoto Ishii
    Laboratory of Human Tumor Viruses, Department of Viral Oncology, Institute for Virus Research, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    J Virol 80:4510-20. 2006
    ..These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents...
  69. ncbi request reprint [Molecular mechanisms on interferon resistance of hepatitis C virus]
    Kazuhito Naka
    Tanpakushitsu Kakusan Koso 49:526-33. 2004
  70. ncbi request reprint Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes
    Kui Li
    Department of Microbiology and Immunology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Biol Chem 280:16739-47. 2005
    ..We conclude that hepatocytes contain two distinct antiviral signaling pathways leading to expression of type I IFNs, one dependent upon TLR3 and the other dependent on RIG-I, with little cross-talk between these pathways...
  71. ncbi request reprint NS3 protein of Hepatitis C virus associates with the tumour suppressor p53 and inhibits its function in an NS3 sequence-dependent manner
    Lin Deng
    Division of Microbiology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    J Gen Virol 87:1703-13. 2006
    ..Our results collectively suggest the possibility that NS3 plays an important role in the hepatocarcinogenesis of HCV by interacting differentially with p53 in an NS3 sequence-dependent manner...
  72. ncbi request reprint Complications of percutaneous radiofrequency ablation for hepato-cellular carcinoma: imaging spectrum and management
    Masaaki Akahane
    Departments of Radiology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Radiographics 25:S57-68. 2005
    ..Because an early and accurate diagnosis is necessary for proper management, radiologists should be familiar with the imaging features of each type of complication...
  73. ncbi request reprint Efficient formation of vesicular stomatitis virus pseudotypes bearing the native forms of hepatitis C virus envelope proteins detected after sonication
    Kazushi Tamura
    Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Microbes Infect 7:29-40. 2005
    ..The VSV pseudotypes developed here would be useful for analyzing the early stages of HCV infection...