Ikuo Igarashi


Affiliation: Obihiro University of Agriculture and Veterinary Medicine
Country: Japan


  1. Rizk M, Aboulaila M, El Sayed S, Guswanto A, Yokoyama N, Igarashi I. Inhibitory effects of fluoroquinolone antibiotics on Babesia divergens and Babesia microti, blood parasites of veterinary and zoonotic importance. Infect Drug Resist. 2018;11:1605-1615 pubmed publisher
    ..These results suggest that safe and cheap fluoroquinolone, such as enoxacin, might be used for the treatment of clinical cases caused by Babesia spp. in animals or humans. ..
  2. Tuvshintulga B, Sivakumar T, Yokoyama N, Igarashi I. Development of unstable resistance to diminazene aceturate in Babesia bovis. Int J Parasitol Drugs Drug Resist. 2019;9:87-92 pubmed publisher
    ..bovis, which may not even respond to subsequent treatments with high doses of DA. Thus, if the bovine babesiosis caused by B. bovis is unresponsive to DA, treatment with other antibabesial agents might be recommended. ..
  3. El Sayed S, Rizk M, Yokoyama N, Igarashi I. Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites. Parasit Vectors. 2019;12:37 pubmed publisher
    ..The obtained results indicate that thymoquinone might be a promising medicinal compound for use in the treatment of animal piroplasmosis. ..
  4. Tayebwa D, Vudriko P, Tuvshintulga B, Guswanto A, Nugraha A, Gantuya S, et al. Molecular epidemiology of Babesia species, Theileria parva, and Anaplasma marginale infecting cattle and the tick control malpractices in Central and Eastern Uganda. Ticks Tick Borne Dis. 2018;9:1475-1483 pubmed publisher
    ..parva, A. marginale and their co-infections were more prevalent in the central than eastern region; even though, tick control malpractices were observed in both regions. Therefore, an urgent tick and TBD control strategy is needed. ..
  5. Tayebwa D, Tuvshintulga B, Guswanto A, Nugraha A, Batiha G, Gantuya S, et al. The effects of nitidine chloride and camptothecin on the growth of Babesia and Theileria parasites. Ticks Tick Borne Dis. 2018;9:1192-1201 pubmed publisher
    ..microti in mice and showed no toxic symptoms in mice. The results indicate that NC may, after further evaluations, prove to be an alternative drug against bovine and equine piroplasmoses. ..
  6. Guswanto A, Nugraha A, Tuvshintulga B, Tayebwa D, Rizk M, Batiha G, et al. 17-DMAG inhibits the multiplication of several Babesia species and Theileria equi on in vitro cultures, and Babesia microti in mice. Int J Parasitol Drugs Drug Resist. 2018;8:104-111 pubmed publisher
    ..The data warrant further evaluation of 17-DMAG as an antibabesial drug and as an option in combination with atovaquone for the treatment of human babesiosis. ..
  7. Tuvshintulga B, Aboulaila M, Davaasuren B, Ishiyama A, Sivakumar T, Yokoyama N, et al. Clofazimine Inhibits the Growth of Babesia and Theileria Parasites In Vitro and In Vivo. Antimicrob Agents Chemother. 2016;60:2739-46 pubmed publisher
  8. Munkhjargal T, Aboge G, Ueno A, Aboulaila M, Yokoyama N, Igarashi I. Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis. Exp Parasitol. 2016;166:29-36 pubmed publisher
    ..The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis. ..
  9. Rizk M, El Sayed S, Aboulaila M, Tuvshintulga B, Yokoyama N, Igarashi I. Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay. Vet Parasitol. 2016;227:93-7 pubmed publisher
    ..divergens that infect cattle. ..

More Information


  1. Yamagishi J, Wakaguri H, Yokoyama N, Yamashita R, Suzuki Y, Xuan X, et al. The Babesia bovis gene and promoter model: an update from full-length EST analysis. BMC Genomics. 2014;15:678 pubmed publisher
    ..These observations allowed the illustration of a model promoter composition, which supports the differences in transcriptional regulation frameworks between apicomplexan parasites and mammals. ..
  2. Munkhjargal T, Yokoyama N, Igarashi I. Recombinant methionine aminopeptidase protein of Babesia microti: immunobiochemical characterization as a vaccine candidate against human babesiosis. Parasitol Res. 2016;115:3669-76 pubmed publisher
    ..Taken together, these results suggest that rBmMetAP1 confers significant protection against experimental B. microti infection and might be considered a potential vaccine target against human babesiosis. ..
  3. Munkhjargal T, Ishizaki T, Guswanto A, Takemae H, Yokoyama N, Igarashi I. Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target. Vet Parasitol. 2016;221:14-23 pubmed publisher
    ..Therefore, our results suggest that BvMAP is a molecular target of amastatin and bestatin, and those inhibitors may be drug candidates for the treatment of babesiosis, though more studies are required to confirm this. ..
  4. Hikosaka K, Tsuji N, Watanabe Y, Kishine H, Horii T, Igarashi I, et al. Novel type of linear mitochondrial genomes with dual flip-flop inversion system in apicomplexan parasites, Babesia microti and Babesia rodhaini. BMC Genomics. 2012;13:622 pubmed publisher
    ..The present finding provides insight into further understanding of evolution of mt genome structure...
  5. El Sayed S, Rizk M, Terkawi M, Yokoyama N, Igarashi I. Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate. Parasitol Int. 2017;66:721-726 pubmed publisher
    ..The obtained data suggest the possible use of rBdEBP as a vaccine candidate against bovine babesiosis. ..
  6. Rizk M, El Sayed S, Aboulaila M, Eltaysh R, Yokoyama N, Igarashi I. Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice. Sci Rep. 2017;7:12774 pubmed publisher
    ..In summary, considering that all experiments were applied under optimal laboratory conditions, fluorescence assay at 2.5% HCT using 2x SG I for B. microti parasite offers a novel approach for drug screening in mice. ..
  7. Tuvshintulga B, Aboulaila M, Sivakumar T, Tayebwa D, Gantuya S, Naranbaatar K, et al. Chemotherapeutic efficacies of a clofazimine and diminazene aceturate combination against piroplasm parasites and their AT-rich DNA-binding activity on Babesia bovis. Sci Rep. 2017;7:13888 pubmed publisher
    ..CF and DA might have an AT-rich DNA-binding activity. All results suggest that the CF-DA combination chemotherapy will be a better choice to treat piroplasmosis instead of DA monotherapy. ..
  8. Guswanto A, Allamanda P, Mariamah E, Sodirun S, Wibowo P, Indrayani L, et al. Molecular and serological detection of bovine babesiosis in Indonesia. Parasit Vectors. 2017;10:550 pubmed publisher
    ..bovis ITS isolates from Indonesia were found to be more genetically diverse than B. bigemina ITS isolates. The data presented in this study are necessary to develop an effective strategy for controlling the disease in the country. ..
  9. Rizk M, El Sayed S, Aboulaila M, Yokoyama N, Igarashi I. Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites. Exp Parasitol. 2017;179:43-48 pubmed publisher
    ..bovis and B. caballi cultures. These results indicate that N-acetyl-L-cysteine might be used as a drug for the treatment of babesiosis, especially when used in combination with diminazene aceturate. ..
  10. Guswanto A, Allamanda P, Mariamah E, Munkjargal T, Tuvshintulga B, Takemae H, et al. Evaluation of immunochromatographic test (ICT) strips for the serological detection of Babesia bovis and Babesia bigemina infection in cattle from Western Java, Indonesia. Vet Parasitol. 2017;239:76-79 pubmed publisher
    ..Furthermore, the data showed the current seroprevalence of bovine babesiosis in Western Java, Indonesia, and efficient control strategies are needed to reduce economic losses due to the disease. ..
  11. Fereig R, Mohamed S, Mahmoud H, AbouLaila M, Guswanto A, Nguyen T, et al. Seroprevalence of Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginale antibodies in cattle in southern Egypt. Ticks Tick Borne Dis. 2017;8:125-131 pubmed publisher
    ..bigemina infection rate. The recorded seroprevalence of hemoprotozoan parasites and A. marginale in cattle suggests that these diseases have the potential capacity to detrimentally affect meat and milk production in southern Egypt. ..