Toshio Iwasaki

Summary

Affiliation: Nippon Medical School
Country: Japan

Publications

  1. pmc Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo 113 8602, Japan
    J Am Chem Soc 134:19731-8. 2012
  2. ncbi request reprint Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Biol Chem 280:9129-34. 2005
  3. ncbi request reprint 15N HYSCORE characterization of the fully deprotonated, reduced form of the archaeal Rieske [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 128:2170-1. 2006
  4. pmc Resonance Raman characterization of archaeal and bacterial Rieske protein variants with modified hydrogen bond network around the [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo, Japan
    Protein Sci 15:2019-24. 2006
  5. pmc Crystallization and preliminary X-ray diffraction studies of a hyperthermophilic Rieske protein variant (SDX-triple) with an engineered rubredoxin-like mononuclear iron site
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:993-5. 2006
  6. pmc Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:1014-6. 2007
  7. pmc Continuous-wave and pulsed EPR characterization of the [2Fe-2S](Cys)3(His)1 cluster in rat MitoNEET
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 131:13659-67. 2009
  8. pmc Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo 113 8602, Japan
    FEBS Lett 583:3467-72. 2009
  9. doi request reprint ISC-like [2Fe-2S] ferredoxin (FdxB) dimer from Pseudomonas putida JCM 20004: structural and electron-nuclear double resonance characterization
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo, 113 8602, Japan
    J Biol Inorg Chem 16:923-35. 2011
  10. ncbi request reprint Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal rieske [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 126:13902-3. 2004

Collaborators

Detail Information

Publications22

  1. pmc Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo 113 8602, Japan
    J Am Chem Soc 134:19731-8. 2012
    ....
  2. ncbi request reprint Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Biol Chem 280:9129-34. 2005
    ....
  3. ncbi request reprint 15N HYSCORE characterization of the fully deprotonated, reduced form of the archaeal Rieske [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 128:2170-1. 2006
    ..These quantitative data can be used in theoretical analysis for the selection of an appropriate model of the mixed-valence state of the reduced Rieske center at very alkaline pH...
  4. pmc Resonance Raman characterization of archaeal and bacterial Rieske protein variants with modified hydrogen bond network around the [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo, Japan
    Protein Sci 15:2019-24. 2006
    ..This is not observed in the case of the hydrogen bond between the bridging sulfide ligand (S(b)) and Ser-Ogamma, which is weak and/or unfavorably oriented for extensive coupling with the Fe-S(b/t) stretching vibrations...
  5. pmc Crystallization and preliminary X-ray diffraction studies of a hyperthermophilic Rieske protein variant (SDX-triple) with an engineered rubredoxin-like mononuclear iron site
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:993-5. 2006
    ..63 A resolution and belong to the triclinic space group P1, with unit-cell parameters a = 43.56, b = 76.54, c = 80.28 A, alpha = 88.12, beta = 78.82, gamma = 73.46 degrees. The asymmetric unit contains eight protein molecules...
  6. pmc Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:1014-6. 2007
    ..5. The thin needle-shaped crystals diffract to 1.90 A resolution and belong to the hexagonal space group P6(1)22, with unit-cell parameters a = 87.58, c = 73.14 A. The asymmetric unit contains one protein molecule...
  7. pmc Continuous-wave and pulsed EPR characterization of the [2Fe-2S](Cys)3(His)1 cluster in rat MitoNEET
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 131:13659-67. 2009
    ..It is suggested that these quantitative data may be used in future functional and theoretical studies on the mammalian mitoNEET [2Fe-2S] cluster system...
  8. pmc Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Tokyo 113 8602, Japan
    FEBS Lett 583:3467-72. 2009
    ..They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems...
  9. doi request reprint ISC-like [2Fe-2S] ferredoxin (FdxB) dimer from Pseudomonas putida JCM 20004: structural and electron-nuclear double resonance characterization
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo, 113 8602, Japan
    J Biol Inorg Chem 16:923-35. 2011
    ..g. adrenodoxin) and suggest a significant distortion of the electronic structure of the reduced [2Fe-2S] cluster under the influence of the protein environment around each iron site in general...
  10. ncbi request reprint Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal rieske [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 126:13902-3. 2004
    ..These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron-proton transfer reactions...
  11. ncbi request reprint Characterization of the pH-dependent resonance Raman transitions of archaeal and bacterial Rieske [2Fe-2S] proteins
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 126:4788-9. 2004
    ....
  12. ncbi request reprint Novel [2Fe-2S]-type redox center C in SdhC of archaeal respiratory complex II from Sulfolobus tokodaii strain 7
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Biol Chem 277:39642-8. 2002
    ..tokodaii respiratory complex II, as compared with those in mitochondrial and some mesophilic bacterial enzymes, indicate modular evolution of this ubiquitous electron entry site in the respiratory chains of aerobic organisms...
  13. ncbi request reprint Engineering a three-cysteine, one-histidine ligand environment into a new hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin from Sulfolobus solfataricus
    Asako Kounosu
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Biol Chem 279:12519-28. 2004
    ....
  14. pmc Crystallization and preliminary X-ray diffraction studies of hyperthermophilic archaeal Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus P1
    Asako Kounosu
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:842-5. 2010
    ..5. The crystals diffracted to 1.85 A resolution and belonged to the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = 60.72, c = 83.31 A. The asymmetric unit contains one protein molecule...
  15. pmc Crystallization and preliminary X-ray diffraction studies of the prototypal homologue of mitoNEET (Tth-NEET0026) from the extreme thermophile Thermus thermophilus HB8
    Asako Kounosu
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1146-8. 2008
    ..085 M HEPES-NaOH pH 7.2. The dark reddish crystals diffracted to 1.80 A resolution and belonged to the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = 45.51, c = 84.26 A. The asymmetric unit contains one protein molecule...
  16. ncbi request reprint Sequence and electron paramagnetic resonance analyses of nitrate reductase NarGH from a denitrifying halophilic euryarchaeote Haloarcula marismortui
    Katsuhiko Yoshimatsu
    Department of Biology and Geosciences, Faculty of Science, Shizuoka University, 836 Ohya, 422 8529, Shizuoka, Japan
    FEBS Lett 516:145-50. 2002
    ..marismortui enzyme is a new archaeal member of the known membrane-bound nitrate reductases whose homologs are found in the bacterial domain...
  17. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske [2Fe-2S] cluster environment
    Takuro Uchiyama
    Department of Life Science, Tokyo Institute of Technology, Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Acta Crystallogr D Biol Crystallogr 60:1487-9. 2004
    ..0 A resolution and belong to the cubic space group F4(1)32, with unit-cell parameter a = 163.00 +/- 0.05 A. The asymmetric unit contains one sulredoxin molecule. Three-wavelength MAD data were collected...
  18. pmc Redox-dependent structural changes in archaeal and bacterial Rieske-type [2Fe-2S] clusters
    Nathaniel J Cosper
    Center for Metalloenzyme Studies, University of Georgia, Athens 30602, USA
    Protein Sci 11:2969-73. 2002
    ....
  19. ncbi request reprint X-ray absorption spectroscopic analysis of reductive [2Fe-2S] cluster degradation in hyperthermophilic archaeal succinate:caldariellaquinone oxidoreductase subunits
    Zhongrui Li
    Department of Chemistry, University of Georgia, Athens, Georgia 30602 2556, USA
    Biochemistry 42:15003-8. 2003
    ....
  20. ncbi request reprint A comparative, two-dimensional 14N ESEEM characterization of reduced [2Fe-2S] clusters in hyperthermophilic archaeal high- and low-potential Rieske-type proteins
    Sergei A Dikanov
    Department of Veterinary Clinical Medicine, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Biol Inorg Chem 9:753-67. 2004
    ....
  21. ncbi request reprint The interaction of the Rieske iron-sulfur protein with occupants of the Qo-site of the bc1 complex, probed by electron spin echo envelope modulation
    Rimma I Samoilova
    Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk 630090, Russia
    J Biol Chem 277:4605-8. 2002
    ....
  22. ncbi request reprint Sulfolobus tokodaii sp. nov. (f. Sulfolobus sp. strain 7), a new member of the genus Sulfolobus isolated from Beppu Hot Springs, Japan
    Toshiharu Suzuki
    Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
    Extremophiles 6:39-44. 2002
    ..We propose that this isolate is a novel species of the genus Sulfolobus that we name Sulfolobus tokodaii sp. nov. The type strain is strain 7 (JCM 10545)...