Affiliation: Nippon Medical School
Hattori Y, Ishii H, Morita A, Sakuma Y, Ozawa H. Characterization of the fundamental properties of the N-terminal truncation (Î” exon 1) variant of estrogen receptor Î± in the rat. Gene. 2015;571:117-25 pubmed publisher
..Therefore, the present study provides fundamental and useful information for further research into the regulation and functions of ERÎ± gene variants. ..
Arai Y, Ishii H, Kobayashi M, Ozawa H. Subunit profiling and functional characteristics of acetylcholine receptors in GT1-7 cells. J Physiol Sci. 2017;67:313-323 pubmed publisher
..These findings demonstrate the molecular profiles of AChRs, which directly contribute to GnRH secretion in GT1-7 cells, and provide one possible regulatory action of acetylcholine in GnRH neurons. ..
Ishii H, Tanaka N, Kobayashi M, Kato M, Sakuma Y. Gene structures, biochemical characterization and distribution of rat melatonin receptors. J Physiol Sci. 2009;59:37-47 pubmed publisher
..This study delineates the gene structures, fundamental properties, and distribution of both rat melatonin receptor subtypes, and may offer opportunities to assess the physiological significance of melatonin in rats. ..
Ishii H, Sato S, Yin C, Sakuma Y, Kato M. Cetrorelix, a gonadotropin-releasing hormone antagonist, induces the expression of melatonin receptor 1a in the gonadotropin-releasing hormone neuronal cell line GT1-7. Neuroendocrinology. 2009;90:251-9 pubmed publisher
..These findings may provide one possible mechanism for the sexually dimorphic responses to melatonin in adult rat GnRH neurons. ..
Ishii H, Kobayashi M, Sakuma Y. Alternative promoter usage and alternative splicing of the rat estrogen receptor alpha gene generate numerous mRNA variants with distinct 5'-ends. J Steroid Biochem Mol Biol. 2010;118:59-69 pubmed publisher
Ishii H, Kobayashi M, Munetomo A, Miyamoto T, Sakuma Y. Novel splicing events and post-transcriptional regulation of human estrogen receptor ? E isoforms. J Steroid Biochem Mol Biol. 2013;133:120-8 pubmed publisher
..Furthermore, the 5'-UTRs of the E isoforms post-transcriptionally control human ER? expression mainly through translational repression. ..
Ishii H, Hattori Y, Munetomo A, Watanabe H, Sakuma Y, Ozawa H. Characterization of rodent constitutively active estrogen receptor ? variants and their constitutive transactivation mechanisms. Gen Comp Endocrinol. 2017;248:16-26 pubmed publisher
..This comparative study documented the complexity of ER? genes, mRNAs, and proteins, and further determined the underlying structural basis of ligand-independent activation by C-terminally-truncated ER? variants. ..