Hirofumi Watanabe

Summary

Affiliation: National Institute of Agrobiological Sciences
Country: Japan

Publications

  1. ncbi request reprint Animal cellulases
    H Watanabe
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
    Cell Mol Life Sci 58:1167-78. 2001
  2. ncbi request reprint New endo-beta-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites
    H Watanabe
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8634, Japan
    Cell Mol Life Sci 59:1983-92. 2002
  3. doi request reprint Cellulolytic systems in insects
    Hirofumi Watanabe
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba, Ibaraki, Japan
    Annu Rev Entomol 55:609-32. 2010
  4. pmc Symbiotic "Archaezoa" of the primitive termite Mastotermes darwiniensis still play a role in cellulase production
    Hirofumi Watanabe
    National Institute of Agrobiological Science, 1 2 Owashi, Tsukuba 305 8634, Japan
    Eukaryot Cell 5:1571-6. 2006
  5. pmc Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches
    Gaku Tokuda
    Center of Molecular Biosciences COMB, University of the Ryukyus, Nishihara, Okinawa 903 0213, Japan
    BMC Res Notes 1:118. 2008
  6. pmc Evidence for the presence of a cellulase gene in the last common ancestor of bilaterian animals
    Nathan Lo
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba 305 8634, Japan
    Proc Biol Sci 270:S69-72. 2003
  7. ncbi request reprint Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability
    Jinfeng Ni
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Protein Eng Des Sel 20:535-42. 2007
  8. ncbi request reprint Heterologous overexpression of a mutant termite cellulase gene in Escherichia coli by DNA shuffling of four orthologous parental cDNAs
    Jinfeng Ni
    National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Biosci Biotechnol Biochem 69:1711-20. 2005
  9. ncbi request reprint Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris
    Masahiro Sugimura
    National Institute of Agrobiological Sciences, Ibaraki, Japan
    Eur J Biochem 270:3455-60. 2003
  10. ncbi request reprint Evidence for cocladogenesis between diverse dictyopteran lineages and their intracellular endosymbionts
    Nathan Lo
    National Institute of Agrobiological Sciences, Tsukuba, Japan
    Mol Biol Evol 20:907-13. 2003

Detail Information

Publications11

  1. ncbi request reprint Animal cellulases
    H Watanabe
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
    Cell Mol Life Sci 58:1167-78. 2001
    ..To date, cellulase genes have been reported from arthropods (insects and a crayfish) and nematodes. This paper describes and discusses the presence and nature of endogenous cellulases in higher animals...
  2. ncbi request reprint New endo-beta-1,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites
    H Watanabe
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8634, Japan
    Cell Mol Life Sci 59:1983-92. 2002
    ..cDNAs encoding putative EGs homologous to GHF7 members were then identified. The functionality of one of the putative proteins was confirmed by its expression in Escherichia coli...
  3. doi request reprint Cellulolytic systems in insects
    Hirofumi Watanabe
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba, Ibaraki, Japan
    Annu Rev Entomol 55:609-32. 2010
    ....
  4. pmc Symbiotic "Archaezoa" of the primitive termite Mastotermes darwiniensis still play a role in cellulase production
    Hirofumi Watanabe
    National Institute of Agrobiological Science, 1 2 Owashi, Tsukuba 305 8634, Japan
    Eukaryot Cell 5:1571-6. 2006
    ..Eukaryot. Cell 2:1091-1098, 2003), the hindgut protozoan fauna of M. darwiniensis actively produce cellulases, which play an important role in cellulose digestion of the host termite...
  5. pmc Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches
    Gaku Tokuda
    Center of Molecular Biosciences COMB, University of the Ryukyus, Nishihara, Okinawa 903 0213, Japan
    BMC Res Notes 1:118. 2008
    ..In the present study, we describe a simple method of endosymbiont purification from complex environment, using Blattabacterium cuenoti inhabiting in cockroaches as a model system...
  6. pmc Evidence for the presence of a cellulase gene in the last common ancestor of bilaterian animals
    Nathan Lo
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba 305 8634, Japan
    Proc Biol Sci 270:S69-72. 2003
    ..Overall, our results suggest that at least one family of endogenous cellulases may be more widespread in animals than previously thought...
  7. ncbi request reprint Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability
    Jinfeng Ni
    National Institute of Agrobiological Sciences, 1 2 Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Protein Eng Des Sel 20:535-42. 2007
    ..In summary, we improved thermostability of the termite cellulase and increased the V(max) value and specific activity by combining only cDNAs encoding enzymes adapted for normal temperatures...
  8. ncbi request reprint Heterologous overexpression of a mutant termite cellulase gene in Escherichia coli by DNA shuffling of four orthologous parental cDNAs
    Jinfeng Ni
    National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Biosci Biotechnol Biochem 69:1711-20. 2005
    ..coli harboring the mutant genes was 20-30 fold higher than that of wild-type genes. The mutant enzyme showed high activity against CMC and properties similar to those of the native enzymes...
  9. ncbi request reprint Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris
    Masahiro Sugimura
    National Institute of Agrobiological Sciences, Ibaraki, Japan
    Eur J Biochem 270:3455-60. 2003
    ..hilaris cellulase showed high homology to members of glycosyl hydrolase family 5 subfamily 2, and, in addition, a signature sequence for family 5 was found. Thus, this is the first report of a family 5 cellulase from arthropods...
  10. ncbi request reprint Evidence for cocladogenesis between diverse dictyopteran lineages and their intracellular endosymbionts
    Nathan Lo
    National Institute of Agrobiological Sciences, Tsukuba, Japan
    Mol Biol Evol 20:907-13. 2003
    ..The results support the idea that the stem group of modern cockroaches radiated sometime between the late Jurassic and early Cretaceous-not the Carboniferous, as has been suggested on the basis of roach-like fossils from this epoch...
  11. doi request reprint Inconspicuous matured males of worker form are produced in orphaned colonies of Reticulitermes speratus (Isoptera: Rhinotermitidae) and participate in reproduction
    Ai Fujita
    National Institute of Agrobiological Sciences, Insect Microbe Research Unit, Tsukuba, Ibaraki, Japan
    J Insect Physiol 56:1510-5. 2010
    ..The presence of the female ergatoid (one type of female neotenic) did not induce the production of inconspicuous reproductive males during the 12-week experiment time...