T Ushijima

Summary

Affiliation: National Cancer Center
Country: Japan

Publications

  1. ncbi request reprint Detection and interpretation of altered methylation patterns in cancer cells
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Nat Rev Cancer 5:223-31. 2005
  2. doi request reprint Stronger prognostic power of the CpG island methylator phenotype than methylation of individual genes in neuroblastomas
    Kiyoshi Asada
    Division of Epigenomics, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Jpn J Clin Oncol 43:641-5. 2013
  3. doi request reprint Prevention of Helicobacter pylori-induced gastric cancers in gerbils by a DNA demethylating agent
    Tohru Niwa
    Division of Epigenomics, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Prev Res (Phila) 6:263-70. 2013
  4. doi request reprint Molecular pathways: involvement of Helicobacter pylori-triggered inflammation in the formation of an epigenetic field defect, and its usefulness as cancer risk and exposure markers
    Toshikazu Ushijima
    Division of Epigenomics, National Cancer Center Research Institute, Tokyo, Japan
    Clin Cancer Res 18:923-9. 2012
  5. pmc A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A
    Francesca Colombo
    Department of Predictive and Preventive Medicine, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
    Mol Cancer 10:81. 2011
  6. ncbi request reprint Aberrant methylations in cancer cells: where do they come from?
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 96:206-11. 2005
  7. ncbi request reprint Chromosomal mapping of genes controlling development, histological grade, depth of invasion, and size of rat stomach carcinomas
    T Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 60:1092-6. 2000
  8. doi request reprint Aberrant DNA methylation in contrast with mutations
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Cancer Sci 101:300-5. 2010
  9. pmc Fidelity of the methylation pattern and its variation in the genome
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Genome Res 13:868-74. 2003
  10. ncbi request reprint Epigenetic field for cancerization
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research, Institute 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    J Biochem Mol Biol 40:142-50. 2007

Collaborators

Detail Information

Publications105 found, 100 shown here

  1. ncbi request reprint Detection and interpretation of altered methylation patterns in cancer cells
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Nat Rev Cancer 5:223-31. 2005
    ..What techniques can be used to identify new tumour suppressors and diagnostic markers?..
  2. doi request reprint Stronger prognostic power of the CpG island methylator phenotype than methylation of individual genes in neuroblastomas
    Kiyoshi Asada
    Division of Epigenomics, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Jpn J Clin Oncol 43:641-5. 2013
    ..The purpose of this study was to compare the prognostic power of the CpG island methylator phenotype with that of methylation of individual genes...
  3. doi request reprint Prevention of Helicobacter pylori-induced gastric cancers in gerbils by a DNA demethylating agent
    Tohru Niwa
    Division of Epigenomics, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Prev Res (Phila) 6:263-70. 2013
    ..pylori-induced gastric cancers and suggested that removal of induced DNA methylation and/or suppression of DNA methylation induction can become a target for prevention of chronic inflammation-associated cancers...
  4. doi request reprint Molecular pathways: involvement of Helicobacter pylori-triggered inflammation in the formation of an epigenetic field defect, and its usefulness as cancer risk and exposure markers
    Toshikazu Ushijima
    Division of Epigenomics, National Cancer Center Research Institute, Tokyo, Japan
    Clin Cancer Res 18:923-9. 2012
    ..It provides translational opportunities for cancer risk diagnosis incorporating life history, assessment of past exposure to carcinogenic factors, and cancer prevention...
  5. pmc A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A
    Francesca Colombo
    Department of Predictive and Preventive Medicine, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
    Mol Cancer 10:81. 2011
    ..Survival discrepancies between Asians and Caucasians point to ethnic differences in allelic frequencies of the functional genetic variations...
  6. ncbi request reprint Aberrant methylations in cancer cells: where do they come from?
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 96:206-11. 2005
    ..Efforts in the field on how aberrant methylation is induced will lead to new cancer prevention, diagnostics, and therapeutics...
  7. ncbi request reprint Chromosomal mapping of genes controlling development, histological grade, depth of invasion, and size of rat stomach carcinomas
    T Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 60:1092-6. 2000
    ....
  8. doi request reprint Aberrant DNA methylation in contrast with mutations
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Cancer Sci 101:300-5. 2010
    ..Methylation is potentially reversible, unlike mutations. These characteristics of methylation are opening up new fields of application and research...
  9. pmc Fidelity of the methylation pattern and its variation in the genome
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Genome Res 13:868-74. 2003
    ..The differential MPERs even among unmethylated CGIs indicated that a promoter-specific protection mechanism(s) from de novo methylation was present...
  10. ncbi request reprint Epigenetic field for cancerization
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research, Institute 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    J Biochem Mol Biol 40:142-50. 2007
    ..These findings emphasize the usefulness of DNA methylation as a marker for past exposure to carcinogens and future risk of cancer development...
  11. ncbi request reprint Single nucleotide instability without microsatellite instability in rat mammary carcinomas
    N Watanabe
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 61:2632-40. 2001
    ....
  12. ncbi request reprint Cloning of the rat agouti gene and identification of the rat nonagouti mutation
    T Kuramoto
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045 Japan
    Mamm Genome 12:469-71. 2001
  13. ncbi request reprint Genetic determinant and environmental carcinogens
    M Nagao
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Mutat Res 402:85-91. 1998
    ..By mapping the gene, we will be able to identify humans who might belong to high risk group in general population, and cancer can be prevented more efficiently by attaining early diagnosis...
  14. pmc Human exposure to carcinogenic heterocyclic amines and their mutational fingerprints in experimental animals
    M Nagao
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Environ Health Perspect 104:497-501. 1996
    ..The mechanisms involved in this development of microsatellite instability in PhlP. The mechanisms involved in this development of microsatellite instability in PhlP-induced cancers remain to be elucidated...
  15. ncbi request reprint LacI transgenic animal study: relationships among DNA-adduct levels, mutant frequencies and cancer incidences
    M Nagao
    Biochemistry Division, National Cancer Center Research Institute, Tsukiji 5, Chuo Ku, 104 0045, Tokyo, Japan
    Mutat Res 477:119-24. 2001
    ..However, there was no direct correlation between MFs and cancer incidences. Possible mechanisms involved in the discordance between DNA damage markers and cancer incidences are discussed...
  16. ncbi request reprint cDNA cloning of the rat APC gene and assignment to chromosome 18
    M Toyota
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Mamm Genome 6:746-8. 1995
  17. ncbi request reprint Silencing of HTR1B and reduced expression of EDN1 in human lung cancers, revealed by methylation-sensitive representational difference analysis
    D Takai
    Carcinogenesis Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
    Oncogene 20:7505-13. 2001
    ..MS-RDA was shown to isolate DNA fragments that are hypermethylated and silenced, such as HTR1B, and those whose expressions are altered and the methylation statuses outside the promoter region are altered, such as EDN1...
  18. doi request reprint FHL1 on chromosome X is a single-hit gastrointestinal tumor-suppressor gene and contributes to the formation of an epigenetic field defect
    K Asada
    Division of Epigenomics, National Cancer Center Research Institute, Tokyo, Japan
    Oncogene 32:2140-9. 2013
    ..These data showed that FHL1 is a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, and that its silencing contributes to the formation of an epigenetic field for cancerization...
  19. ncbi request reprint Cloning of rat Brca2 and linkage mapping to chromosome 12
    S Yamada
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Mamm Genome 8:850-1. 1997
  20. doi request reprint Alu and SatĪ± hypomethylation in Helicobacter pylori-infected gastric mucosae
    Takeichi Yoshida
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Int J Cancer 128:33-9. 2011
    ..pylori infection during gastric carcinogenesis, possibly in different target cells, and that global hypomethylation is not always present in human gastric cancers...
  21. ncbi request reprint Linkage mapping of the rat Tp53 gene on chromosome 10
    F Canzian
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104, Japan
    Mamm Genome 7:630. 1996
  22. ncbi request reprint p53 mutations in MeIQ-induced mouse forestomach tumors
    H Makino
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Nihon Ika Daigaku Zasshi 64:39-44. 1997
    ..These results thus indicate that p53 mutations may occur relatively early but do not confer any predisposition for lymph node metastasis...
  23. ncbi request reprint Organ variation in the mutagenicity of MeIQ in Big Blue lacI transgenic mice
    T Suzuki
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan
    Mutat Res 369:45-9. 1996
    ..8 times higher in the bone marrow, 4.6 times higher in the liver, and 2.6 times higher in the forestomach. No increase in MF was detected in the heart, where no tumors develop...
  24. ncbi request reprint Linkage mapping of the rat interleukin 1 receptor antagonist gene on chromosome 3
    T Kuramoto
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Exp Anim 50:355-7. 2001
  25. ncbi request reprint Decreased fidelity in replicating DNA methylation patterns in cancer cells leads to dense methylation of a CpG island
    N Watanabe
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Curr Top Microbiol Immunol 310:199-210. 2006
    ..2-fold). Presence of densely methylated DNA molecules was observed only in KATOIII and AGS. These data demonstrated that some cancer cells have decreased fidelity in replicating CpG methylation patterns that underlie CIMP...
  26. pmc Attractin/mahogany/zitter plays a critical role in myelination of the central nervous system
    T Kuramoto
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Proc Natl Acad Sci U S A 98:559-64. 2001
    ..We conclude from these results that the membrane Atrn has a critical role in normal myelination in the CNS and would provide insights into the physiology of myelination as well as the etiology of myelin diseases...
  27. ncbi request reprint Genetic alterations of androgen receptor gene in Japanese human prostate cancer
    M Watanabe
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Jpn J Clin Oncol 27:389-93. 1997
    ..This study indicates that AR gene mutations play a role, in only a subset of prostate cancer patients, in a treatment-refractory state...
  28. doi request reprint The presence of a methylation fingerprint of Helicobacter pylori infection in human gastric mucosae
    Takeshi Nakajima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Int J Cancer 124:905-10. 2009
    ..These findings open up the possibility that a methylation fingerprint can be used as a novel marker for past exposure to a specific carcinogenic factor...
  29. ncbi request reprint The presence of single nucleotide instability in human breast cancer cell lines
    N Watanabe
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan
    Cancer Res 61:7739-42. 2001
    ..These findings showed that single nucleotide instability was also present in five of the six human breast cancer cell lines and strongly indicates it has important roles in human and rat mammary carcinogenesis...
  30. doi request reprint DNA methylation of microRNA genes in gastric mucosae of gastric cancer patients: its possible involvement in the formation of epigenetic field defect
    Takayuki Ando
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Int J Cancer 124:2367-74. 2009
    ..This strongly indicated that methylation-silencing of miRNA genes, in addition to that of protein-coding genes, contributed to the formation of a field defect for gastric cancers...
  31. ncbi request reprint Larger numbers of silenced genes in cancer cell lines with increased de novo methylation of scattered CpG sites
    Kazuki Moriguchi
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Lett 249:178-87. 2007
    ..The association between the increase of methylation seeds and larger numbers of silenced genes suggested that the increase can induce gene silencing overriding their transcription...
  32. doi request reprint Inflammatory processes triggered by Helicobacter pylori infection cause aberrant DNA methylation in gastric epithelial cells
    Tohru Niwa
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Res 70:1430-40. 2010
    ....
  33. ncbi request reprint Decreased expression of the seven ARP2/3 complex genes in human gastric cancers
    Atsushi Kaneda
    National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Cancer Lett 212:203-10. 2004
    ..The consistent decrease of the Arp2/3 complex genes and its important role in actin organization suggested that the decrease could be involved in cancer phenotypes, such as dysplastic morphology...
  34. ncbi request reprint DNA methylation as a marker for the past and future
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    J Gastroenterol 41:401-7. 2006
    ..DNA methylation is emerging as a promising marker for past exposure to carcinogens and future risk of cancers...
  35. ncbi request reprint High levels of aberrant DNA methylation in Helicobacter pylori-infected gastric mucosae and its possible association with gastric cancer risk
    Takao Maekita
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji, Tokyo, Japan
    Clin Cancer Res 12:989-95. 2006
    ..Here, we analyzed the effect of H. pylori infection on the quantity of methylated DNA molecules in noncancerous gastric mucosae and examined its association with gastric cancer risk...
  36. ncbi request reprint Whole-genome analyses of loss of heterozygosity and methylation analysis of four tumor-suppressor genes in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat stomach carcinomas
    Tohru Niwa
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Cancer Sci 96:409-13. 2005
    ..The search for other genes involved in these carcinomas needs to be continued...
  37. ncbi request reprint Genetic and epigenetic alterations in carcinogenesis
    T Sugimura
    Carcinogenesis Division, National Cancer Center, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Mutat Res 462:235-46. 2000
    ..Based on the growing understanding of the roles of DNA methylation, several new methodologies were developed to make a genome-wide search for changes in DNA methylation. Now, a wave of new findings is in sight...
  38. ncbi request reprint Higher methylation levels in gastric mucosae significantly correlate with higher risk of gastric cancers
    Takeshi Nakajima
    Endoscopy Division, National Cancer Center Hospital, Tokyo, Japan
    Cancer Epidemiol Biomarkers Prev 15:2317-21. 2006
    ..pylori-negative healthy individuals. Here, using cases with multiple gastric cancers, we analyzed whether the higher methylation levels correlated with the higher risk of gastric cancers...
  39. pmc Methylation of multiple genes in gastric glands with intestinal metaplasia: A disorder with polyclonal origins
    Mami Mihara
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji 5 chome, Tokyo 104, Japan
    Am J Pathol 169:1643-51. 2006
    ....
  40. ncbi request reprint Decreased fidelity in replicating CpG methylation patterns in cancer cells
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 65:11-7. 2005
    ..These data showed that some cancer cells have decreased fidelity in replicating methylation patterns in some CGIs, and that the decrease could lead to methylation of the entire CGIs...
  41. ncbi request reprint Diagnostic and therapeutic applications of epigenetics
    Kazuaki Miyamoto
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo, 104 0045 Japan
    Jpn J Clin Oncol 35:293-301. 2005
    ..Histone deacetylase inhibitors and methods for gene-specific epigenetic modification are being developed. Application of epigenetics to cancer diagnostics and therapeutics, and possibly to cancer prevention, is coming into clinics...
  42. ncbi request reprint Establishment of a detection system for demethylating agents using an endogenous promoter CpG island
    Eriko Okochi-Takada
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Mutat Res 568:187-94. 2004
    ..We believe that this is the first assay system that detects agents that disturb the methylated status of a CGI that regulates an endogenous promoter...
  43. doi request reprint Persistence of a component of DNA methylation in gastric mucosae after Helicobacter pylori eradication
    Takeshi Nakajima
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    J Gastroenterol 45:37-44. 2010
    ..We aimed to demonstrate the decrease in methylation levels...
  44. ncbi request reprint Studies on mammary carcinogenesis induced by a heterocyclic amine, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, in mice and rats
    Minako Nagao
    Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan
    Environ Mol Mutagen 39:158-64. 2002
    ..These mutational characteristics were also observed in the original tumors. Single-nucleotide instability (SNI) might be implicated in the mammary cancer induced by PhIP...
  45. ncbi request reprint Reduced Brca1 protein expression in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced rat mammary carcinomas
    Eriko Okochi
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Mol Carcinog 34:211-8. 2002
    ..As underlying mechanisms, alterations in posttranscriptional regulation or stability of Brca1 protein were suggested...
  46. ncbi request reprint Single nucleotide instability: a wide involvement in human and rat mammary carcinogenesis?
    Eriko Okochi
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji 5 chome, Chuoku, Tokyo, Japan
    Mutat Res 506:101-11. 2002
    ..These human breast cancer cell lines were proficient in G/T mismatch binding, and were reported to lack MSI. SNI was suggested to play a wide involvement in human and rat mammary carcinogenesis...
  47. ncbi request reprint Fibroblasts associated with cancer cells keep enhanced migration activity after separation from cancer cells: a novel character of tumor educated fibroblasts
    Genichiro Ishii
    Pathology Division, Research Center for Innovative Oncology, Kashiwa, Chiba 277 8577, Japan
    Int J Oncol 37:317-25. 2010
    ....
  48. pmc Development of a novel output value for quantitative assessment in methylated DNA immunoprecipitation-CpG island microarray analysis
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    DNA Res 16:275-86. 2009
    ..The Me value was considered to be useful for analysis of intermediate methylation levels of CGIs...
  49. ncbi request reprint [DNA methylation and cancer--DNA methylation as a target of cancer chemotherapy]
    Kazuaki Miyamoto
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Gan To Kagaku Ryoho 30:2021-9. 2003
    ..Finally, reports are coming out on methods that can induce sequence-specific methylation changes. Rapid progress in this field is expected...
  50. ncbi request reprint Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae
    Shotaro Enomoto
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 98:1853-61. 2007
    ..These showed that the CIMP statuses in gastric cancers had no association with methylation levels in the background gastric mucosae...
  51. doi request reprint Early-stage formation of an epigenetic field defect in a mouse colitis model, and non-essential roles of T- and B-cells in DNA methylation induction
    M Katsurano
    Division of Epigenomics, National Cancer Center Research Institute, Tokyo, Japan
    Oncogene 31:342-51. 2012
    ..These results showed that an epigenetic field defect is formed at early stages of colitis-associated carcinogenesis and that functional T and B cells are non-essential for the formation...
  52. ncbi request reprint Frequent hypomethylation in multiple promoter CpG islands is associated with global hypomethylation, but not with frequent promoter hypermethylation
    Atsushi Kaneda
    Carcinogenesis Division and Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 95:58-64. 2004
    ....
  53. doi request reprint Induction of epigenetic alterations by chronic inflammation and its significance on carcinogenesis
    Tohru Niwa
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Adv Genet 71:41-56. 2010
    ..Expression of DNA methyltransferases (DNMTs) is not necessarily induced by inflammation, and local imbalance between DNMTs and factors that protect genes from DNA methylation seems to be important...
  54. ncbi request reprint Etiology-specific gene expression profiles in rat mammary carcinomas
    Takashi Kuramoto
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
    Cancer Res 62:3592-7. 2002
    ..Our results indicated that gene expression profiles in cancers reflect their etiology and suggested a possibility that etiology of cancers could be retrospectively estimated from their expression profiles...
  55. doi request reprint Methylation silencing of transforming growth factor-beta receptor type II in rat prostate cancers
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Cancer Res 68:2112-21. 2008
    ....
  56. ncbi request reprint CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas
    Masanobu Abe
    Carcinogenesis Division, National Cancer Center Research Institute, University of Tokyo Graduate School of Medicine, 5 1 1 Tsikiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Res 65:828-34. 2005
    ..The results showed that neuroblastomas with CIMP have a poor prognosis and suggested induction of silencing of important genes as an underlying mechanism...
  57. ncbi request reprint Lysyl oxidase is a tumor suppressor gene inactivated by methylation and loss of heterozygosity in human gastric cancers
    Atsushi Kaneda
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer Res 64:6410-5. 2004
    ..These data showed that LOX is a tumor suppressor gene inactivated by methylation and loss of heterozygosity in gastric cancers, and possibly also in other cancers...
  58. doi request reprint Methylation-sensitive representational difference analysis (MS-RDA)
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Methods Mol Biol 507:117-30. 2009
    ..The difference can be identified by RDA. Most technical difficulties reside in the RDA procedure, and many fine techniques are necessary for a successful application of this powerful technology...
  59. ncbi request reprint Focus on gastric cancer
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan 104 0045
    Cancer Cell 5:121-5. 2004
  60. ncbi request reprint Genetic mapping of the RET protooncogene on rat chromosome 4
    F Canzian
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Mamm Genome 6:433-5. 1995
  61. pmc Specific 5'-GGGA-3'-->5'-GGA-3' mutation of the Apc gene in rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
    H Kakiuchi
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Proc Natl Acad Sci U S A 92:910-4. 1995
    ..PhIP-induced tumors are characterized by their specific and unique mutation, which may be useful for mutational fingerprinting of human cancers...
  62. ncbi request reprint cDNA cloning and characterization of ret activated in a human papillary thyroid carcinoma cell line
    Y Ishizaka
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Biochem Biophys Res Commun 168:402-8. 1990
    ..The aberrant tyrosine kinase activity induced by the rearrangement of ret proto-oncogene could be involved in the development of papillary thyroid carcinoma...
  63. ncbi request reprint [Epigenome and human disease]
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute
    Nihon Rinsho 67:1219-26. 2009
    ..Multiple reports show that epigenomic changes are present in acquired neurological, metabolic, and immunological disorders, and more research in the field is urgently necessary...
  64. ncbi request reprint Persistence of gene expression changes in stomach mucosae induced by short-term N-methyl-N'-nitro-N-nitrosoguanidine treatment and their presence in stomach cancers
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Mutat Res 549:185-93. 2004
    ..Persistent expression changes are considered to be important for prediction of past carcinogen exposure, and could provide a molecular environment favorable for malignant transformation...
  65. ncbi request reprint Global expression analysis of N-methyl-N'-nitro-N-nitrosoguanidine-induced rat stomach carcinomas using oligonucleotide microarrays
    Masanobu Abe
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji 5 1 1, Chuo Ku, Tokyo 104 0045, Japan
    Carcinogenesis 24:861-7. 2003
    ....
  66. ncbi request reprint Methylation-sensitive representational difference analysis and its application to cancer research
    Atsushi Kaneda
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
    Ann N Y Acad Sci 983:131-41. 2003
    ..MS-RDA is effective in identifying silenced genes in various cancers...
  67. doi request reprint Association between frequent CpG island methylation and HER2 amplification in human breast cancers
    Kotoe Terada
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Carcinogenesis 30:466-71. 2009
    ..001). These showed that frequent methylation had a strong association with HER2 amplification in breast cancers and suggested that frequent methylation can be a determinant of various characteristics in a fraction of human breast cancers...
  68. doi request reprint The presence of aberrant DNA methylation in noncancerous esophageal mucosae in association with smoking history: a target for risk diagnosis and prevention of esophageal cancers
    Daiji Oka
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Cancer 115:3412-26. 2009
    ..In this study, the objective was to elucidate the effects of smoking and drinking on the accumulation of epigenetic alterations in background mucosae...
  69. doi request reprint Adenomatous polyposis coli 1A is likely to be methylated as a passenger in human gastric carcinogenesis
    Kosuke Hosoya
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Lett 285:182-9. 2009
    ..These findings strongly indicated that methylation of APC promoter 1A is a passenger, and suggested that marked down-regulation of 1B expression could be related to formation of a field predisposed to gastric cancers...
  70. pmc The presence of RNA polymerase II, active or stalled, predicts epigenetic fate of promoter CpG islands
    Hideyuki Takeshima
    Carcinogenesis Division, National Cancer Center Research Institute, 104 0045 Tokyo, Japan
    Genome Res 19:1974-82. 2009
    ..These results showed that the presence of Pol II, active or stalled, and H3K27me3 can predict the epigenetic fate of promoter CGIs independently of transcription levels...
  71. ncbi request reprint Identification of silencing of nine genes in human gastric cancers
    Atsushi Kaneda
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
    Cancer Res 62:6645-50. 2002
    ..It is expected that these nine genes may include important genes in gastric cancer development and would be useful to identify a distinct subset of gastric cancers...
  72. ncbi request reprint Reduced expression of the insulin-induced protein 1 and p41 Arp2/3 complex genes in human gastric cancers
    Atsushi Kaneda
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Int J Cancer 100:57-62. 2002
    ..INSIG1 and p41-Arc are known to be involved in cellular differentiation and morphology, respectively, and it was suggested that their reduced expressions might be involved in gastric cancer development or progression...
  73. ncbi request reprint [Acquired disorders and epigenetics]
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Gan To Kagaku Ryoho 37:1645-9. 2010
    ..Further research in the field is necessary to clarify the causal roles of these epigenetic alterations in disease development, and to apply the findings to new strategies of disease prevention, diagnosis, and treatment...
  74. ncbi request reprint Silencing of tissue factor pathway inhibitor-2 gene in malignant melanomas
    Yoshimasa Nobeyama
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo, Japan
    Int J Cancer 121:301-7. 2007
    ..The results showed that TFPI-2 is silenced in human malignant melanomas by methylation of its promoter CGI and suggested that its silencing is involved in melanoma metastasis...
  75. ncbi request reprint Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers
    Li yi Cai
    Carcinogenesis Division, National Cancer Center Research Institute, Japan
    Life Sci 80:1458-65. 2007
    ..Silencing of PRTFDC1 was revealed here for the first time, and aberrant methylation of GPR150, ITGA8 and HOXD11 could be candidate tumor markers...
  76. ncbi request reprint Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas
    Junichi Furuta
    Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji, Tokyo, Japan
    Cancer Res 66:6080-6. 2006
    ..On the other hand, 12 CGIs were methylated in >or=9 of the 13 melanoma cell lines and are considered as candidate melanoma biomarkers...
  77. ncbi request reprint Silencing of the thrombomodulin gene in human malignant melanoma
    Junichi Furuta
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Melanoma Res 15:15-20. 2005
    ..These findings suggest that most human melanomas lack TM expression, and that methylation of the promoter CpG island is one of the mechanisms responsible...
  78. ncbi request reprint Chemical genomic screening for methylation-silenced genes in gastric cancer cell lines using 5-aza-2'-deoxycytidine treatment and oligonucleotide microarray
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 97:64-71. 2006
    ..Cancer Sci 2006; 97: 64 -71)...
  79. ncbi request reprint Methylation and expression analysis of 15 genes and three normally-methylated genes in 13 Ovarian cancer cell lines
    Masayoshi Imura
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Lett 241:213-20. 2006
    ..It was shown that aberrant methylation and demethylation were frequently observed in ovarian cancer cell lines, and these data will provide a basis for further epigenetic analysis in ovarian cancers...
  80. ncbi request reprint Identification of 20 genes aberrantly methylated in human breast cancers
    Kazuaki Miyamoto
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Int J Cancer 116:407-14. 2005
    ..This may serve as markers for early-stage breast cancers and suggests that aberrant methylation targets transcriptionally inactive genes in vivo...
  81. ncbi request reprint Cloning of the 5' upstream region of the rat p16 gene and its role in silencing
    Masanobu Abe
    Carcinogenesis Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Jpn J Cancer Res 93:1100-6. 2002
    ....
  82. ncbi request reprint Methylation destiny: Moira takes account of histones and RNA polymerase II
    Hideyuki Takeshima
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
    Epigenetics 5:89-95. 2010
    ..Now, epigenetic destiny can be predicted by these factors and interference with these factors might be able to change the destiny...
  83. ncbi request reprint Methylation-associated silencing of heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2 (3-OST-2) in human breast, colon, lung and pancreatic cancers
    Kazuaki Miyamoto
    National Cancer Center Research Institute, Tokyo, Japan
    Oncogene 22:274-80. 2003
    ..From the high incidence, silencing of the 3-OST-2 gene is expected to have high diagnostic, and potentially therapeutic, values...
  84. ncbi request reprint [Genes involved in breast cancers]
    Toshikazu Ushijima
    Carcinogenesis Division, National Cancer Center Research Institute
    Nihon Rinsho 64:432-6. 2006
    ..p53 mutations are present in 20-25% of sporadic breast cancers, and inactivation of its pathway is present in most of them. Further molecular analysis will reveal new targets for diagnosis and therapeutics...
  85. ncbi request reprint Identification of 27 5' CpG islands aberrantly methylated and 13 genes silenced in human pancreatic cancers
    Atsushi Hagihara
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Oncogene 23:8705-10. 2004
    ..MSP of 24 primary pancreatic cancers showed that all these genes, except for THBD, were methylated in at least one cancer. Some of those were suggested to be potentially involved in pancreatic cancer development and progression...
  86. ncbi request reprint Marked and independent prognostic significance of the CpG island methylator phenotype in neuroblastomas
    Masanobu Abe
    Carcinogenesis Division, National Cancer Center Research Institute, University of Tokyo Graduate School of Medicine, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Lett 247:253-8. 2007
    ..In multivariate analysis, CIMP and N-myc amplification had an influence on OS and DFS independent of age and disease stage. CIMP had a stronger influence on DFS than N-myc amplification while N-myc had a stronger influence on OS...
  87. ncbi request reprint Reduced expression of GNA11 and silencing of MCT1 in human breast cancers
    Kiyoshi Asada
    Division of Carcinogenesis, National Cancer Center Research Institute, Tokyo, Japan
    Oncology 64:380-8. 2003
    ..MCT1 is involved in cellular transportation of butyrate, which induces cellular differentiation. Downregulation of these two genes was suggested to be involved in human breast cancers...
  88. ncbi request reprint Promoter methylation profiling of 30 genes in human malignant melanoma
    Junichi Furuta
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
    Cancer Sci 95:962-8. 2004
    ..No correlation between frequent methylation and frequent demethylation was observed. These profiles showed that both aberrant methylation and demethylation occur widely in human melanomas...
  89. doi request reprint Identification of genes targeted by CpG island methylator phenotype in neuroblastomas, and their possible integrative involvement in poor prognosis
    Masanobu Abe
    Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan
    Oncology 74:50-60. 2008
    ..Methylation of nonpromoter CGIs is useful to detect the presence of the CIMP, while the poor prognosis is considered to be caused by gene silencing due to promoter methylation. Here, promoter CGIs targeted by the CIMP were searched for...
  90. ncbi request reprint Linkage and microarray analyses of susceptibility genes in ACI/Seg rats: a model for prostate cancers in the aged
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo, Japan
    Cancer Res 65:2610-6. 2005
    ..These results showed that prostate cancer susceptibility of ACI rats involves at least four loci, and suggested Mme and Cdkn1a as candidates for Pcr1 and Pcs2...
  91. pmc A specific CpG site demethylation in the human interleukin 2 gene promoter is an epigenetic memory
    Akiko Murayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan
    EMBO J 25:1081-92. 2006
    ..Our observations indicate that Oct-1-binding followed by CpG demethylation are key events in the epigenetic regulation of IL2 expression and may act as a memory of the regulatory event...
  92. ncbi request reprint beta-Catenin mutations and nuclear accumulation during progression of rat stomach adenocarcinomas
    Tetsuya Tsukamoto
    Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681
    Cancer Sci 94:1046-51. 2003
    ....
  93. ncbi request reprint No-observed effect levels for carcinogenicity and for in vivo mutagenicity of a genotoxic carcinogen
    Manabu Hoshi
    Department of Pathology, Department of Orthopaedic Surgery, Osaka City University Medical School, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    Toxicol Sci 81:273-9. 2004
    ..Thus, no observed effect level (NOEL) was demonstrated for both carcinogenicity in terms of preneoplastic lesion induction and in vivo mutagenicity of MeIQx, and the NOEL for in vivo mutagenicity was lower than that for carcinogenicity...
  94. pmc Expression quantitative trait loci analysis of 13 genes in the rat prostate
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Genetics 171:1231-8. 2005
    ..eQTL analysis with precise measurement of expression levels and appropriate normalization was shown to be effective for mapping loci that control gene expression in vivo...
  95. ncbi request reprint Cyclin D1 overexpression in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric adenocarcinomas
    Shinji Takasu
    Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    Exp Toxicol Pathol 59:171-5. 2007
    ..Cyclin D1 was also found to be accumulated in gastric glands within normal-looking mucosa, these perhaps representing preneoplastic lesions for cancers with membranous beta-catenin accumulation...
  96. ncbi request reprint Profiling and selection of genes differentially expressed in the pylorus of rat strains with different proliferative responses and stomach cancer susceptibility
    Satoshi Yamashita
    Carcinogenesis Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    Carcinogenesis 23:923-8. 2002
    ....
  97. ncbi request reprint Down-regulation of human X-box binding protein 1 (hXBP-1) expression correlates with tumor progression in human prostate cancers
    Satoru Takahashi
    First Department of Pathology, Nagoya City University Medical School, Mizuho cho, Mizuho ku, Nagoya, Japan
    Prostate 50:154-61. 2002
    ..In the present investigation, we characterized alterations of hXBP-1 in prostate cancer specimens...
  98. ncbi request reprint Causal relationship between the loss of RUNX3 expression and gastric cancer
    Qing Lin Li
    Department of Biochemistry, College of Medicine, Institute of Medical Research, Chungbuk National University, 361 763, Cheongju, South Korea
    Cell 109:113-24. 2002
    ....
  99. pmc RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD
    Xin Zi Chi
    Department of Biochemistry, School of Medicine, and Institute for Tumor Research, Chungbuk National University, Cheongju 361 763, South Korea
    Mol Cell Biol 25:8097-107. 2005
    ..Our results suggest that at least part of the tumor suppressor activity of RUNX3 is associated with its ability to induce p21 expression...
  100. ncbi request reprint Methylation-associated silencing of the Wnt antagonist SFRP1 gene in human ovarian cancers
    Toshio Takada
    Carcinogenesis Division, National Cancer Center Hospital, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Sci 95:741-4. 2004
    ..These results show that SFRP1 is inactivated by promoter methylation in human ovarian cancers, as well as colon cancers...
  101. ncbi request reprint [Genetic alterations and expression changes in breast cancers]
    Kotoe Terada
    Carcinogenesis Division, National Cancer Center Research Institute
    Nihon Rinsho 65:113-9. 2007